RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Cusk ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Brosme brosme
FDA Seafood List Common Name: Cusk
FDA Seafood List Market Name: Cusk
Unique IEF Gel Code: 19930823gel05
Unique Web Code: ckh01Srr
Tissue Aliquot Code: brosbros 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	102	11.9	0.0736698	4	0.542306	5.3	4.4	4.55	102.2	4.3	0.0710467
2	5.2	202	23.5	0.18156	4	1.17862	11.6	9.5	5.2	201.7	3.7	0.179172
3	5.85	316	36.7	0.21623	4	1.47639	14.5	11.9	5.85	316.2	3.9	0.213431
4	6.55	445	51.7	0.145283	6	1.50163	14.7	12.1	6.55	445.3	5.9	0.142911
5	6.85	477	55.5	0.0267204	4	0.181206	1.8	1.5	6.85	477.6	4.1	0.0249609
6	7.35	543	63.1	0.0575331	5	0.481539	4.7	3.9	7.35	543.6	4.7	0.0577431
7	8.15	662	77	0.0867551	7	1.10557	10.8	8.9	8.15	662.5	7.4	0.0838379
8	8.45	723	84.1	0.100909	6	1.16598	11.4	9.4	8.45	723.3	6.5	0.101393
9	8.65	777	90.3	0.0666347	4	0.484282	4.7	3.9	8.65	776.2	4.1	0.066032
10	9.3	827	96.2	0.199397	6	2.07918	20.4	16.7	9.3	827.2	6.1	0.193359

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.014	28	3.3	0.0509118	6	0.519068	2.2	1.7	4.014	27.9	6	0.048863
2	4.195	55	6.4	0.049586	2	0.227396	0.9	0.8	4.195	50.9	2.4	0.0545678
3	4.302	71	8.3	0.239011	9	3.52468	14.7	11.6	4.302	73.2	8.9	0.22235
4	4.55	108	12.6	0.0360541	18	0.694156	2.9	2.3	4.55	103.3	18	0.0217579
5	4.691	129	15	0.145814	4	0.943279	3.9	3.1	4.691	128.8	3.7	0.142497
6	5.2	205	23.8	0.0597919	7	0.735052	3.1	2.4	5.2	204.1	6.8	0.0610019
7	5.313	224	26	0.529487	3	2.45934	10.2	8.1	5.313	224	3	0.468076
8	5.444	246	28.6	0.0206974	10	1.40861	5.9	4.6	5.444	229.5	9.8	0.0814916
9	5.528	260	30.2	0.0358888	3	0.171385	0.7	0.6	5.528	260.3	2.7	0.0355498
10	5.576	268	31.2	0.0221585	3	0.130469	0.5	0.4	5.576	267.6	3.3	0.0223521
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.629	277	32.2	0.0294867	2	0.102979	0.4	0.3	5.629	276.6	1.9	0.030455
12	5.725	293	34.1	0.460497	4	2.79242	11.6	9.2	5.725	292.5	3.6	0.441352
13	5.796	305	35.5	0.102564	5	0.823442	3.4	2.7	5.796	304.5	4.8	0.0969174
14	5.85	314	36.5	0.103678	6	1.01232	4.2	3.3	5.85	314.7	5.6	0.101471
15	6.125	365	42.4	0.0652888	4	0.395898	1.6	1.3	6.125	366.1	4	0.0563411
16	6.195	378	44	0.0370247	5	0.257355	1.1	0.8	6.195	377.2	4.5	0.0319126
17	6.259	390	45.3	0.29682	2	1.21154	5	4	6.259	390.1	2.3	0.300123
18	6.388	414	48.1	0.0785682	5	0.714496	3	2.4	6.388	413.9	5.3	0.075911
19	6.437	423	49.2	0.121413	4	0.752981	3.1	2.5	6.437	423.4	3.9	0.108093
20	6.534	441	51.3	0.0255061	12	0.547683	2.3	1.8	6.534	435.5	12.5	0.0247595
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.85	477	55.5	0.0158587	5	0.136593	0.6	0.5	6.85	477.9	5.1	0.0152135
22	7.35	541	62.9	0.0998296	3	0.35804	1.5	1.2	7.35	540.8	3.5	0.0579216
23	7.436	554	64.4	0.0919166	17	2.64791	11	8.7	7.436	555.5	17.1	0.0873981
24	8.45	731	85	0.0268271	6	0.309151	1.3	1	8.45	730.6	6.4	0.0270684
25	8.65	778	90.5	0.0207337	4	0.145169	0.6	0.5	8.65	777.6	4	0.0204121
26	9.3	832	96.7	0.109012	6	1.00428	4.2	3.3	9.3	831.3	5.6	0.10202

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.009	25	2.9	0.0695447	6	0.701931	3.7	2.9	4.009	25.2	5.8	0.0685112
2	4.199	53	6.2	0.0627096	5	0.550767	2.9	2.2	4.199	51.4	5.1	0.0613168
3	4.302	68	7.9	0.258588	6	2.02066	10.7	8.3	4.302	69.1	5.6	0.205329
4	4.567	107	12.4	0.0483336	18	1.81208	9.6	7.4	4.567	83.5	18	0.0567985
5	4.69	125	14.5	0.174594	4	1.2306	6.5	5	4.69	125.4	4	0.174948
6	5.206	201	23.3	0.0284572	4	0.198698	1	0.8	5.206	200.8	4	0.0278842
7	5.306	218	25.3	0.48158	3	2.43369	12.8	9.9	5.306	218.2	3.1	0.438912
8	5.418	237	27.5	0.0305055	10	1.15927	6.1	4.7	5.418	226.8	9.7	0.0672879
9	5.518	254	29.5	0.0296722	3	0.126428	0.7	0.5	5.518	253.8	2.5	0.0282934
10	5.559	261	30.3	0.0293614	4	0.192291	1	0.8	5.559	261.2	3.7	0.0296898
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.612	270	31.4	0.0359775	3	0.16874	0.9	0.7	5.612	269.6	2.7	0.0353582
12	5.706	286	33.2	0.423578	3	2.40034	12.7	9.8	5.706	285.3	3.2	0.421402
13	5.776	298	34.6	0.0859155	6	0.912259	4.8	3.7	5.776	297.8	6.1	0.0837384
14	6.104	359	41.7	0.033907	2	0.119	0.6	0.5	6.104	358.7	1.9	0.0350495
15	6.146	367	42.6	0.0112898	2	0.0349861	0.2	0.1	6.146	365.3	1.6	0.0119797
16	6.178	373	43.3	0.027311	5	0.211761	1.1	0.9	6.178	372.5	4.8	0.0251487
17	6.235	384	44.6	0.262925	2	1.13455	6	4.6	6.235	384	2.4	0.262929
18	6.361	408	47.4	0.0759772	6	0.788572	4.2	3.2	6.361	407.6	6.2	0.0718917
19	6.408	417	48.4	0.1049	5	0.773987	4.1	3.2	6.408	417.6	4.5	0.0964758
20	7.412	549	63.8	0.0799208	15	1.9923	10.5	8.1	7.412	551.5	14.8	0.075808

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	100	11.6	0.0922172	4	0.640621	5.5	4.5	4.55	100.3	4	0.0898011
2	5.2	194	22.6	0.168264	5	1.33722	11.6	9.3	5.2	194.3	4.5	0.167465
3	5.85	306	35.6	0.221623	5	1.80212	15.6	12.5	5.85	305.9	4.7	0.215878
4	6.55	443	51.6	0.147683	7	1.74603	15.1	12.2	6.55	441.7	7	0.140913
5	6.85	472	54.9	0.0337381	5	0.277103	2.4	1.9	6.85	472.9	4.9	0.0318822
6	7.35	537	62.5	0.0770452	4	0.591453	5.1	4.1	7.35	537.3	4.3	0.0774185
7	8.15	663	77.2	0.1074	6	1.16904	10.1	8.1	8.15	662.4	6.2	0.1066
8	8.45	725	84.4	0.115571	6	1.19176	10.3	8.3	8.45	725	5.8	0.116741
9	8.65	776	90.3	0.0862016	4	0.599552	5.2	4.2	8.65	775.4	4.1	0.0832887
10	9.3	831	96.7	0.248119	5	2.20068	19	15.3	9.3	830.2	5.1	0.244919

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.042	22	2.6	0.0621501	5	0.582965	2.6	2.1	4.042	22.8	5.4	0.0608172
2	4.239	51	5.9	0.0879007	5	0.688916	3.1	2.5	4.239	50.2	4.5	0.0859491
3	4.354	68	7.9	0.24596	7	2.79996	12.5	10.3	4.354	69.8	7.2	0.218196
4	4.55	97	11.3	0.0832217	18	2.68696	12	9.8	4.55	92.2	17.7	0.0858577
5	4.726	123	14.3	0.15728	4	1.17929	5.2	4.3	4.726	123.3	4.4	0.1522
6	5.2	193	22.4	0.0489455	6	0.516042	2.3	1.9	5.2	192.1	6.1	0.0480504
7	5.325	214	24.8	0.322598	4	2.22803	9.9	8.2	5.325	213.7	4.1	0.307782
8	5.444	234	27.1	0.0257938	12	0.887773	3.9	3.3	5.444	224.9	11.9	0.0421145
9	5.534	249	28.9	0.0216181	2	0.0541732	0.2	0.2	5.534	249.2	2.1	0.0148885
10	5.588	258	29.9	0.0324773	5	0.292451	1.3	1.1	5.588	258.2	5.3	0.0314181
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.623	264	30.6	0.0306971	3	0.113508	0.5	0.4	5.623	265	2.6	0.0249688
12	5.725	281	32.6	0.368465	3	2.08053	9.3	7.6	5.725	280.5	3.3	0.358599
13	5.79	292	33.9	0.0546417	4	0.455039	2	1.7	5.79	291.3	4.3	0.0590202
14	5.85	302	35	0.0821035	4	0.62211	2.8	2.3	5.85	302	4.3	0.080958
15	6.125	357	41.4	0.031593	3	0.146832	0.7	0.5	6.125	357.3	2.6	0.0323607
16	6.175	367	42.6	0.0191024	2	0.0395542	0.2	0.1	6.175	365.5	1.5	0.0144748
17	6.2	372	43.2	0.0275848	7	0.304152	1.4	1.1	6.2	372.8	7.3	0.0235372
18	6.265	385	44.7	0.226009	3	1.03594	4.6	3.8	6.265	384.6	2.6	0.221347
19	6.375	407	47.2	0.0741472	6	0.738666	3.3	2.7	6.375	406.6	5.9	0.0706129
20	6.42	416	48.3	0.120448	4	0.847108	3.8	3.1	6.42	416	4.2	0.113347
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.49	430	49.9	0.0273445	5	0.262515	1.2	1	6.49	432.6	5.4	0.0272273
22	6.85	474	55	0.0095997	7	0.0976293	0.4	0.4	6.85	475.5	6.7	0.0082285
23	7.35	539	62.5	0.109027	10	1.72883	7.7	6.3	7.35	541.3	9.7	0.100766
24	8.155	664	77	0.0226364	18	0.643419	2.9	2.4	8.155	656	18	0.0201676
25	8.45	736	85.4	0.0343313	6	0.364602	1.6	1.3	8.45	735.5	5.9	0.0348628
26	8.65	779	90.4	0.0265577	4	0.176393	0.8	0.6	8.65	778.7	3.7	0.02665
27	9.3	829	96.2	0.0858033	7	0.909399	4	3.3	9.3	827.8	6.7	0.0770437

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.069	21	2.4	0.042786	5	0.35518	2.1	1.5	4.069	20.2	4.9	0.0411884
2	4.242	47	5.5	0.0061808	3	0.0325432	0.2	0.1	4.242	47.5	2.5	0.0072052
3	4.341	62	7.2	0.187595	5	1.79649	10.7	7.6	4.341	62.1	5.5	0.185975
4	4.46	80	9.3	0.0598089	11	1.13375	6.7	4.8	4.46	79.4	11.1	0.0574476
5	4.719	119	13.9	0.135003	5	1.12475	6.7	4.8	4.719	119.4	4.6	0.137875
6	5.215	194	22.6	0.0233697	3	0.148695	0.9	0.6	5.215	193.8	3.5	0.0241831
7	5.31	210	24.4	0.395136	4	2.6878	15.9	11.4	5.31	210.2	4	0.383187
8	5.507	243	28.3	0.025284	3	0.139634	0.8	0.6	5.507	242.6	3.2	0.0244153
9	5.561	252	29.3	0.0156853	4	0.140526	0.8	0.6	5.561	250.1	4.3	0.0182846
10	5.597	258	30	0.0222824	3	0.120716	0.7	0.5	5.597	258.4	3.2	0.0213474
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.692	274	31.9	0.360938	3	2.1324	12.6	9.1	5.692	274.2	3.3	0.359749
12	5.764	286	33.3	0.0421362	4	0.345028	2	1.5	5.764	286.2	4.4	0.0443095
13	6.107	352	41	0.0147591	2	0.0360755	0.2	0.2	6.107	351.2	1.5	0.0132342
14	6.156	362	42.1	0.0272784	15	0.971184	5.8	4.1	6.156	371.2	15.4	0.0356764
15	6.256	382	44.5	0.196051	3	0.881727	5.2	3.7	6.256	382.2	3	0.166479
16	6.331	397	46.2	0.0766691	11	1.49083	8.8	6.3	6.331	398.6	10.8	0.0776675
17	6.391	409	47.6	0.152253	6	1.16995	6.9	5	6.391	410	5.9	0.111523
18	7.312	533	62	0.0891587	15	2.15724	12.8	9.2	7.312	535.5	15.2	0.080014

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	93	10.8	0.0490953	4	0.380272	4.6	3.8	4.55	93.8	4.5	0.0478494
2	5.2	193	22.3	0.0855332	6	0.89105	10.8	9	5.2	192.3	5.8	0.0868276
3	5.85	302	35	0.137922	6	1.39895	16.9	14.1	5.85	302.3	5.6	0.139909
4	6.55	443	51.3	0.12635	5	1.17709	14.3	11.9	6.55	442.8	5.3	0.125858
5	6.85	475	55	0.0106799	5	0.0927352	1.1	0.9	6.85	473.7	4.7	0.0112272
6	7.35	540	62.5	0.0501359	5	0.433797	5.3	4.4	7.35	539.9	4.8	0.0508665
7	8.15	665	77	0.0836079	7	1.04055	12.6	10.5	8.15	664.1	7.1	0.0824949
8	8.45	736	85.2	0.0873936	6	0.890759	10.8	9	8.45	735.5	5.7	0.0877046
9	8.65	783	90.6	0.0699569	4	0.487487	5.9	4.9	8.65	782.4	4	0.0690606
10	9.3	830	96.1	0.145672	6	1.46257	17.7	14.8	9.3	829.8	5.8	0.14146

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 121

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.300 5.200 11.600 5.850 14.500 6.550 14.700 6.850 1.800 7.350 4.700 8.150 10.800 8.450 11.400 8.650 4.700 9.300 20.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.014 2.200 4.195 0.900 4.302 14.700 4.550 2.900 4.691 3.900 5.200 3.100 5.313 10.200 5.444 5.900 5.528 0.700 5.576 0.500 5.629 0.400 5.725 11.600 5.796 3.400 5.850 4.200 6.125 1.600 6.195 1.100 6.259 5.000 6.388 3.000 6.437 3.100 6.534 2.300 6.850 0.600 7.350 1.500 7.436 11.000 8.450 1.300 8.650 0.600 9.300 4.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 26 Return to Lane Navigator
4.009 3.700 4.199 2.900 4.302 10.700 4.567 9.600 4.690 6.500 5.206 1.000 5.306 12.800 5.418 6.100 5.518 0.700 5.559 1.000 5.612 0.900 5.706 12.700 5.776 4.800 6.104 0.600 6.146 0.200 6.178 1.100 6.235 6.000 6.361 4.200 6.408 4.100 7.412 10.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 5.500 5.200 11.600 5.850 15.600 6.550 15.100 6.850 2.400 7.350 5.100 8.150 10.100 8.450 10.300 8.650 5.200 9.300 19.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.042 2.600 4.239 3.100 4.354 12.500 4.550 12.000 4.726 5.200 5.200 2.300 5.325 9.900 5.444 3.900 5.534 0.200 5.588 1.300 5.623 0.500 5.725 9.300 5.790 2.000 5.850 2.800 6.125 0.700 6.175 0.200 6.200 1.400 6.265 4.600 6.375 3.300 6.420 3.800 6.490 1.200 6.850 0.400 7.350 7.700 8.155 2.900 8.450 1.600 8.650 0.800 9.300 4.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
4.069 2.100 4.242 0.200 4.341 10.700 4.460 6.700 4.719 6.700 5.215 0.900 5.310 15.900 5.507 0.800 5.561 0.800 5.597 0.700 5.692 12.600 5.764 2.000 6.107 0.200 6.156 5.800 6.256 5.200 6.331 8.800 6.391 6.900 7.312 12.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 4.600 5.200 10.800 5.850 16.900 6.550 14.300 6.850 1.100 7.350 5.300 8.150 12.600 8.450 10.800 8.650 5.900 9.300 17.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Cusk ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Cusk ---- Go to Display Gel Lane Navigator.