RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Blue Crab ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Callinectes sapidus
FDA Seafood List Common Name: Blue Crab
FDA Seafood List Market Name: Crab, Blue
Unique IEF Gel Code: 19970213gel04
Unique Web Code: cbh01Srr
Tissue Aliquot Code: callsapi K96-032 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	36	4.6	0.0581473	4	0.36589	5.9	4.3	4.55	35.9	3.7	0.055709
2	5.2	126	15.9	0.0638185	5	0.528558	8.5	6.2	5.2	126.3	5.1	0.0589145
3	5.85	239	30.3	0.0705012	4	0.50357	8.1	6	5.85	238.5	4	0.0704491
4	6.55	380	48.1	0.116705	6	1.1583	18.6	13.7	6.55	379.6	6.2	0.105827
5	6.85	415	52.5	0.0193153	5	0.161637	2.6	1.9	6.85	415.1	5.2	0.0175503
6	7.35	480	60.8	0.0511975	4	0.365248	5.9	4.3	7.35	480	4.1	0.0503028
7	8.15	604	76.5	0.0724295	8	0.953607	15.3	11.3	8.15	603.3	7.6	0.0705366
8	8.45	669	84.7	0.100723	7	1.21853	19.6	14.4	8.45	668.4	6.8	0.100488
9	8.65	719	91	0.0863973	4	0.64795	10.4	7.7	8.65	718.8	4.3	0.0847576
10	9.3	764	96.7	0.0252063	8	0.322855	5.2	3.8	9.3	763.7	8.1	0.0225759

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.533	28	3.6	0.0251588	4	0.16678	1.3	0.6	4.533	28.2	4	0.0236598
2	4.64	44	5.6	0.0435973	3	0.260951	2	0.9	4.64	43.7	3.2	0.046266
3	4.74	59	7.5	0.0616785	5	0.468016	3.6	1.7	4.74	58.1	4.5	0.0583166
4	4.82	71	9	0.0395688	5	0.525425	4	1.9	4.82	74.3	5.2	0.0571092
5	4.88	80	10.2	0.0528146	4	0.199821	1.5	0.7	4.88	80.6	3.6	0.0309086
6	5.04	104	13.2	0.0639532	4	0.439432	3.4	1.6	5.04	104.6	4.5	0.0553655
7	5.242	135	17.1	0.0251524	11	0.341977	2.6	1.2	5.242	137.8	10.8	0.017904
8	5.297	144	18.3	0.177235	5	1.30195	9.9	4.6	5.297	144.9	4.9	0.151269
9	5.363	155	19.7	0.47031	6	4.8059	36.6	17.1	5.363	155.2	6	0.449077
10	5.496	177	22.5	0.0096342	2	0.037379	0.3	0.1	5.496	177.1	2.4	0.0087434
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.684	208	26.4	0.0115525	3	0.0602368	0.5	0.2	5.684	207.9	3.2	0.0104793
12	5.965	259	32.9	0.0901929	10	1.40313	10.7	5	5.965	260.8	10.1	0.0784107
13	6.077	282	35.8	0.0355683	5	0.261478	2	0.9	6.077	280.9	5.1	0.0292068
14	6.184	304	38.6	0.0889723	4	0.55825	4.3	2	6.184	304.1	4	0.0784136
15	6.223	312	39.6	0.0680124	2	0.283017	2.2	1	6.223	311.6	2.4	0.0667311
16	6.248	317	40.2	0.100742	3	0.480896	3.7	1.7	6.248	317.2	2.7	0.101964
17	6.379	344	43.7	0.105316	6	1.06859	8.1	3.8	6.379	343.2	6.2	0.0979571
18	6.814	408	51.8	0.0628827	5	0.453854	3.5	1.6	6.814	408.3	4.5	0.0564665

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	25	3.2	0.0294717	7	0.355152	5.3	4	4.55	25	6.6	0.0302889
2	5.2	130	16.5	0.0462503	9	0.663626	9.8	7.5	5.2	127.8	8.9	0.0419123
3	5.85	232	29.4	0.0654865	5	0.595524	8.8	6.7	5.85	231.6	5.3	0.0639005
4	6.55	378	48	0.136741	7	1.48646	22	16.8	6.55	377.4	6.6	0.126936
5	6.85	409	51.9	0.0274541	6	0.242367	3.6	2.7	6.85	409.5	5.7	0.024
6	7.35	472	59.9	0.0669007	3	0.394507	5.8	4.5	7.35	472.3	3.4	0.0655127
7	8.15	601	76.3	0.0677439	8	0.965408	14.3	10.9	8.15	600.4	8.2	0.0665923
8	8.45	665	84.4	0.105956	6	1.17868	17.4	13.4	8.45	664.5	6.4	0.103672
9	8.65	716	90.9	0.0980144	4	0.697234	10.3	7.9	8.65	715.4	4.2	0.0929556
10	9.3	765	97.1	0.03035	3	0.176262	2.6	2	9.3	765	3.3	0.0303818

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.641	41	5.2	0.0446799	6	0.438091	3.8	2.1	4.641	42.2	6	0.0413717
2	4.763	61	7.7	0.0258295	4	0.174096	1.5	0.8	4.763	59.7	3.7	0.0265403
3	4.842	74	9.4	0.0186696	4	0.11223	1	0.5	4.842	72.9	3.5	0.0180405
4	4.884	81	10.3	0.0321283	5	0.247006	2.1	1.2	4.884	81.8	4.8	0.0293013
5	5.03	105	13.3	0.0368429	4	0.265534	2.3	1.3	5.03	104.7	4.4	0.0338095
6	5.2	133	16.9	0.0431721	4	0.325696	2.8	1.5	5.2	132.4	4.3	0.0430738
7	5.25	141	17.9	0.11023	4	1.0376	9	4.9	5.25	143.2	4.2	0.138202
8	5.33	154	19.5	0.442439	6	4.32021	37.4	20.4	5.33	154.2	5.6	0.435078
9	5.46	175	22.2	0.0079166	3	0.0439568	0.4	0.2	5.46	174.8	3.2	0.0076583
10	5.547	189	24	0.0065567	3	0.0338341	0.3	0.2	5.547	189	3	0.00631
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.621	201	25.5	0.0088841	2	0.0343185	0.3	0.2	5.621	200.7	2.3	0.0083624
12	5.85	238	30.2	0.0294994	3	0.16013	1.4	0.8	5.85	237.8	3	0.0298489
13	5.915	251	31.9	0.0324197	7	0.403257	3.5	1.9	5.915	253.7	6.9	0.0328194
14	6.024	273	34.6	0.0179314	2	0.0750891	0.6	0.4	6.024	273.4	2.4	0.0173348
15	6.153	299	37.9	0.0570581	3	0.26592	2.3	1.3	6.153	298.7	2.8	0.0544862
16	6.193	307	39	0.0411274	3	0.203743	1.8	1	6.193	306.8	2.9	0.039202
17	6.227	314	39.8	0.0686558	2	0.288446	2.5	1.4	6.227	313.7	2.5	0.0662596
18	6.376	344	43.7	0.0724409	6	0.700823	6.1	3.3	6.376	344.4	5.7	0.0691203
19	6.55	379	48.1	0.0414613	7	0.479405	4.1	2.3	6.55	377.1	7.5	0.0362638
20	6.85	408	51.8	0.0456785	7	0.446521	3.9	2.1	6.85	410.1	6.8	0.0370413
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	482	61.2	0.0286988	4	0.190399	1.6	0.9	7.35	481.2	3.7	0.0290376
22	8.15	598	75.9	0.0282708	6	0.319756	2.8	1.5	8.15	597.8	6.4	0.0283809
23	8.45	662	84	0.0338952	7	0.387507	3.4	1.8	8.45	661.1	6.6	0.0329217
24	8.65	714	90.6	0.0240786	4	0.16676	1.4	0.8	8.65	713.5	4.1	0.0232142
25	9.3	765	97.1	0.0205942	12	0.436336	3.8	2.1	9.3	765.7	12.1	0.0203788

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.563	27	3.4	0.0101085	2	0.0397183	0.3	0.1	4.563	27.2	2.2	0.0100396
2	4.627	37	4.7	0.0660969	4	0.394704	3.1	1.4	4.627	37.7	3.6	0.0626036
3	4.749	56	7.1	0.0501074	4	0.378823	3	1.3	4.749	55.8	4.3	0.0496423
4	4.851	72	9.2	0.0477805	3	0.139995	1.1	0.5	4.851	70.5	3.1	0.0253556
5	4.902	80	10.2	0.0505828	10	0.748929	5.8	2.6	4.902	79.2	9.8	0.0430198
6	5.056	104	13.2	0.0690706	9	0.928413	7.2	3.2	5.056	104.1	9	0.0578831
7	5.288	141	17.9	0.171293	5	1.3998	10.9	4.9	5.288	142.1	4.9	0.16209
8	5.354	152	19.3	0.454864	6	4.58229	35.7	16	5.354	152.1	5.8	0.443169
9	5.475	172	21.9	0.0112895	3	0.04403	0.3	0.2	5.475	173.2	2.5	0.0098816
10	5.572	188	23.9	0.0109943	3	0.0603439	0.5	0.2	5.572	187.9	3.1	0.0109235
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.644	200	25.4	0.0169873	3	0.09862	0.8	0.3	5.644	200.6	3.1	0.0178159
12	5.923	249	31.7	0.0819711	9	1.06766	8.3	3.7	5.923	249.7	9	0.0670347
13	6.041	273	34.7	0.0346235	9	0.376333	2.9	1.3	6.041	270.3	8.9	0.0239437
14	6.163	298	37.9	0.0773079	4	0.45328	3.5	1.6	6.163	297.5	3.7	0.0689555
15	6.202	306	38.9	0.0563756	3	0.277554	2.2	1	6.202	306.1	2.8	0.0553064
16	6.237	313	39.8	0.0803025	3	0.389439	3	1.4	6.237	312.4	2.8	0.0798202
17	6.374	341	43.4	0.115498	5	1.0515	8.2	3.7	6.374	340.7	5.4	0.110458
18	6.807	404	51.4	0.0675361	3	0.394111	3.1	1.4	6.807	404.4	3.5	0.0642985

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	25	3.2	0.0492393	4	0.345721	5.4	4.2	4.55	26	4.1	0.0471261
2	5.2	121	15.4	0.060558	6	0.580729	9.1	7	5.2	121.2	5.6	0.0585411
3	5.85	231	29.4	0.0670134	5	0.524802	8.2	6.3	5.85	230.5	4.5	0.0657424
4	6.55	376	47.9	0.103911	7	1.12361	17.6	13.5	6.55	375.4	6.5	0.096815
5	6.85	410	52.2	0.0219095	6	0.204529	3.2	2.5	6.85	410.2	5.8	0.0199757
6	7.35	479	61	0.0513234	4	0.350042	5.5	4.2	7.35	478.5	4	0.0494818
7	8.15	597	76.1	0.0764964	9	1.06733	16.7	12.8	8.15	596.8	8.7	0.0689402
8	8.45	661	84.2	0.10185	7	1.258	19.7	15.1	8.45	660.5	7.1	0.0998448
9	8.65	713	90.8	0.0802928	5	0.646497	10.1	7.8	8.65	712.1	4.7	0.0777183
10	9.3	759	96.7	0.0216744	9	0.295061	4.6	3.5	9.3	756	9.4	0.0177942

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 91

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.900 5.200 8.500 5.850 8.100 6.550 18.600 6.850 2.600 7.350 5.900 8.150 15.300 8.450 19.600 8.650 10.400 9.300 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.533 1.300 4.640 2.000 4.740 3.600 4.820 4.000 4.880 1.500 5.040 3.400 5.242 2.600 5.297 9.900 5.363 36.600 5.496 0.300 5.684 0.500 5.965 10.700 6.077 2.000 6.184 4.300 6.223 2.200 6.248 3.700 6.379 8.100 6.814 3.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 5.300 5.200 9.800 5.850 8.800 6.550 22.000 6.850 3.600 7.350 5.800 8.150 14.300 8.450 17.400 8.650 10.300 9.300 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.641 3.800 4.763 1.500 4.842 1.000 4.884 2.100 5.030 2.300 5.200 2.800 5.250 9.000 5.330 37.400 5.460 0.400 5.547 0.300 5.621 0.300 5.850 1.400 5.915 3.500 6.024 0.600 6.153 2.300 6.193 1.800 6.227 2.500 6.376 6.100 6.550 4.100 6.850 3.900 7.350 1.600 8.150 2.800 8.450 3.400 8.650 1.400 9.300 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 25 Return to Lane Navigator
4.563 0.300 4.627 3.100 4.749 3.000 4.851 1.100 4.902 5.800 5.056 7.200 5.288 10.900 5.354 35.700 5.475 0.300 5.572 0.500 5.644 0.800 5.923 8.300 6.041 2.900 6.163 3.500 6.202 2.200 6.237 3.000 6.374 8.200 6.807 3.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 5.400 5.200 9.100 5.850 8.200 6.550 17.600 6.850 3.200 7.350 5.500 8.150 16.700 8.450 19.700 8.650 10.100 9.300 4.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Blue Crab ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Blue Crab ---- Go to Display Gel Lane Navigator.