RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pollachius virens
FDA Seafood List Common Name: Pollock
FDA Seafood List Market Name: Pollock
Unique IEF Gel Code: 19940912gel07
Unique Web Code: apg01Srr
Tissue Aliquot Code: pollvire H94-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c Co-Migrated	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	51	4.4	0.196784	17	4.99046	16.4	13.5	3.5	48.3	16.8	0.167763
2	4.55	197	17	0.240321	7	2.88383	9.5	7.8	4.55	198.6	7.5	0.21744
3	5.2	277	23.9	0.296776	8	3.68772	12.1	10	5.2	278.7	7.5	0.27595
4	5.85	437	37.7	0.380306	5	3.60736	11.9	9.8	5.85	437.5	5.3	0.386602
5	6.55	612	52.8	0.373289	5	3.08898	10.2	8.4	6.55	611.4	4.8	0.362071
6	6.85	652	56.3	0.154743	7	1.72786	5.7	4.7	6.85	652.4	6.9	0.142086
7	7.35	744	64.2	0.340392	5	3.06639	10.1	8.3	7.35	743.6	5.1	0.338073
8	8.15	905	78.1	0.0656333	10	1.07515	3.5	2.9	8.15	903.7	9.5	0.0637563
9	8.45	965	83.3	0.0968908	7	1.08106	3.6	2.9	8.45	965.3	6.5	0.0934688
10	8.65	1004	86.6	0.0937538	5	0.87361	2.9	2.4	8.65	1003.7	5.5	0.0896911
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1134	97.8	0.527996	5	4.34044	14.3	11.8	9.3	1132.9	5.1	0.477774

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.133	141	12.2	0.681421	5	6.5638	17.2	14	4.133	142.3	5.2	0.712248
2	4.598	208	18	0.0667782	10	1.16841	3.1	2.5	4.598	208.6	9.9	0.0665459
3	4.711	222	19.2	0.216051	5	1.84524	4.8	3.9	4.711	222.7	5	0.207701
4	4.792	232	20.1	0.0889682	3	0.452511	1.2	1	4.792	232.2	3.2	0.080648
5	4.93	249	21.5	0.437988	4	3.37528	8.9	7.2	4.93	248.9	4.3	0.444515
6	5.059	265	22.9	0.0342211	3	0.163084	0.4	0.3	5.059	265	2.7	0.034095
7	5.148	276	23.9	0.0306945	2	0.152946	0.4	0.3	5.148	276	2	0.0431454
8	5.202	283	24.5	0.0504142	3	0.286244	0.8	0.6	5.202	282	3.2	0.0500495
9	5.244	293	25.3	0.51496	4	3.58328	9.4	7.7	5.244	293.6	4.4	0.459185
10	5.311	309	26.7	0.117628	10	2.66325	7	5.7	5.311	302.8	10.3	0.145562
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.395	329	28.5	0.119425	3	0.612594	1.6	1.3	5.395	329.1	3	0.114806
12	5.45	342	29.6	0.0212941	6	0.200201	0.5	0.4	5.45	340.6	5.7	0.0198502
13	5.554	367	31.7	0.0243547	3	0.142972	0.4	0.3	5.554	366.8	3.4	0.023789
14	5.63	385	33.3	0.0540313	5	0.387552	1	0.8	5.63	384.9	4.5	0.0483531
15	5.684	398	34.4	0.0723607	7	0.90723	2.4	1.9	5.684	398.2	7.3	0.0699521
16	5.747	413	35.7	0.0743899	6	0.788948	2.1	1.7	5.747	413.5	6.4	0.0699817
17	5.84	435	37.6	0.0230024	3	0.113671	0.3	0.2	5.84	435.1	2.9	0.022401
18	5.918	454	39.3	0.62713	4	4.43678	11.7	9.5	5.918	455.5	4.3	0.587487
19	6.029	481	41.6	0.419356	3	2.27394	6	4.9	6.029	481.5	3.2	0.40423
20	6.099	498	43.1	0.128629	3	0.806123	2.1	1.7	6.099	498.3	3.4	0.132219
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.128	505	43.7	0.329997	3	1.83672	4.8	3.9	6.128	504.7	3.4	0.302546
22	6.161	513	44.4	0.0859492	3	0.716816	1.9	1.5	6.161	511	2.8	0.142434
23	6.243	533	46.1	0.00994803	3	0.0528988	0.1	0.1	6.243	532.5	2.9	0.0102074
24	6.297	546	47.2	0.181464	2	0.643172	1.7	1.4	6.297	545.9	2	0.182126
25	6.379	566	49	0.0218802	12	0.57515	1.5	1.2	6.379	579.8	12	0.0270416
26	6.457	585	50.6	0.292295	2	0.942491	2.5	2	6.457	585.3	1.9	0.273147
27	6.507	597	51.6	0.0341896	2	0.0729822	0.2	0.2	6.507	596.8	1.6	0.0257871
28	6.567	610	52.8	0.0229193	3	0.12091	0.3	0.3	6.567	609.2	3.2	0.0213362
29	8.814	1034	89.4	0.105536	12	2.17123	5.7	4.6	8.814	1028.6	12	0.102084

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	50	4.3	0.121709	8	1.63228	2.7	2.3	3.5	51	7.9	0.1167
2	4.146	149	12.9	0.675741	5	6.21701	10.3	8.8	4.146	149.7	5	0.69923
3	4.439	194	16.8	0.0176193	2	0.0770736	0.1	0.1	4.439	193.6	2.5	0.01743
4	4.55	211	18.2	0.274935	6	2.43954	4	3.5	4.55	211	5.8	0.23842
5	4.743	235	20.3	0.199347	6	1.89724	3.1	2.7	4.743	235.4	5.7	0.189205
6	4.823	245	21.2	0.0876703	2	0.32721	0.5	0.5	4.823	245.2	2.4	0.0773114
7	4.943	260	22.5	0.397823	4	2.6513	4.4	3.8	4.943	260.4	3.7	0.407087
8	5.023	270	23.3	0.0253664	3	0.0877494	0.1	0.1	5.023	272	3	0.0164966
9	5.104	280	24.2	0.0807102	4	0.585943	1	0.8	5.104	279.2	4.1	0.0800924
10	5.2	292	25.2	0.238111	4	1.71424	2.8	2.4	5.2	291.8	4	0.240955
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.265	307	26.5	0.523511	5	4.29642	7.1	6.1	5.265	307.2	4.6	0.531854
12	5.404	339	29.3	0.143183	3	0.788441	1.3	1.1	5.404	338.9	3.1	0.144153
13	5.451	350	30.2	0.0249893	4	0.198335	0.3	0.3	5.451	348	4.5	0.0251005
14	5.49	359	31	0.0157279	4	0.0903612	0.1	0.1	5.49	360.7	3.8	0.0133639
15	5.573	378	32.6	0.0180314	2	0.0640347	0.1	0.1	5.573	377.8	2	0.0181838
16	5.681	403	34.8	0.0676166	8	0.858756	1.4	1.2	5.681	404	8.2	0.0589662
17	5.72	412	35.6	0.0566198	5	0.372099	0.6	0.5	5.72	415.1	5.1	0.0415232
18	5.781	426	36.8	0.0432047	4	0.324481	0.5	0.5	5.781	425.1	4.3	0.0426273
19	5.85	442	38.2	0.440503	3	2.64177	4.4	3.8	5.85	441.7	3.3	0.447322
20	5.939	463	40	0.554577	6	5.36884	8.9	7.6	5.939	464.9	5.7	0.529282
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.049	489	42.2	0.4418	5	3.42324	5.7	4.9	6.049	488.6	4.6	0.415408
22	6.155	514	44.4	0.191928	7	1.36041	2.3	1.9	6.155	510.3	6.5	0.117853
23	6.172	518	44.7	0.210222	5	1.51064	2.5	2.1	6.172	517.6	5.1	0.168696
24	6.317	552	47.7	0.153936	2	0.595233	1	0.8	6.317	552.3	2.1	0.156286
25	6.495	594	51.3	0.265082	3	1.23722	2	1.8	6.495	593.5	3	0.235274
26	6.55	607	52.4	0.196044	8	2.66391	4.4	3.8	6.55	607.3	7.9	0.190627
27	6.85	655	56.6	0.145009	6	1.37587	2.3	2	6.85	654.5	5.9	0.130885
28	7.35	745	64.3	0.422313	5	3.55537	5.9	5	7.35	744.2	4.8	0.419954
29	8.15	901	77.8	0.0431285	18	2.43765	4	3.5	8.15	885.4	18	0.0764069
30	8.45	962	83.1	0.0543585	8	0.722834	1.2	1	8.45	962.3	8.1	0.0500829
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	1003	86.6	0.107972	6	0.82828	1.4	1.2	8.65	1003.7	5.7	0.0818612
32	8.812	1035	89.4	0.0716644	18	2.21805	3.7	3.1	8.812	1029.9	18	0.0695236
33	9.3	1131	97.7	0.450274	8	5.90063	9.8	8.4	9.3	1129.7	7.7	0.432215

PeakCounter: 33

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	51	4.4	0.194126	18	5.13945	16.4	13.3	3.5	47.7	18	0.160651
2	4.55	199	17.2	0.356189	5	2.5556	8.1	6.6	4.55	199	4.6	0.311037
3	5.2	281	24.3	0.487098	4	3.62833	11.6	9.4	5.2	280.6	4.3	0.478763
4	5.85	432	37.3	0.581175	3	3.44754	11	8.9	5.85	431.9	3.3	0.583281
5	6.55	611	52.8	0.444052	4	3.05069	9.7	7.9	6.55	611	4.1	0.424609
6	6.85	649	56	0.160669	6	1.6039	5.1	4.2	6.85	649.6	6.5	0.14004
7	7.35	741	64	0.373355	5	3.03023	9.7	7.8	7.35	740.7	4.7	0.367508
8	8.15	899	77.6	0.065719	10	1.14015	3.6	3	8.15	899.3	10.2	0.062778
9	8.45	963	83.2	0.0944795	7	1.07482	3.4	2.8	8.45	963.5	6.5	0.0929166
10	8.65	1003	86.6	0.0989768	5	0.910581	2.9	2.4	8.65	1002.3	5.4	0.09542
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1112	96	0.316062	11	5.80122	18.5	15	9.3	1111.3	11.4	0.28626

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.159	147	12.7	0.682066	6	6.75747	14.1	12.2	4.159	148.6	5.6	0.686236
2	4.342	173	15	0.0245835	13	1.17937	2.5	2.1	4.342	160.8	13.2	0.0503769
3	4.476	192	16.6	0.010434	2	0.0288232	0.1	0.1	4.476	192.5	2.1	0.00780416
4	4.617	211	18.2	0.0405761	3	0.118231	0.2	0.2	4.617	208.4	3.5	0.019086
5	4.673	218	18.8	0.0730652	8	0.914264	1.9	1.7	4.673	218.3	7.6	0.067723
6	4.8	234	20.2	0.239589	4	1.79979	3.8	3.3	4.8	234.2	4.1	0.244763
7	5.006	260	22.5	0.50475	4	3.39206	7.1	6.1	5.006	260	3.7	0.514141
8	5.156	279	24.1	0.108797	3	0.548854	1.1	1	5.156	278.7	3.1	0.0998782
9	5.224	290	25.1	0.0955805	5	0.800971	1.7	1.4	5.224	291.5	4.6	0.0987202
10	5.292	306	26.4	0.525941	5	5.11897	10.7	9.3	5.292	305.8	5.3	0.541449
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.348	319	27.6	0.190826	6	2.0868	4.4	3.8	5.348	318.9	6.3	0.186201
12	5.425	337	29.1	0.144895	3	0.762639	1.6	1.4	5.425	337.3	2.9	0.146137
13	5.472	348	30.1	0.0248222	5	0.215185	0.5	0.4	5.472	347.6	5	0.0241586
14	5.519	359	31	0.0130302	2	0.0445417	0.1	0.1	5.519	359.8	2.1	0.0120323
15	5.587	375	32.4	0.0290188	4	0.22168	0.5	0.4	5.587	374.4	4.3	0.0291891
16	5.655	391	33.8	0.0653983	5	0.585709	1.2	1.1	5.655	390.9	4.9	0.0674436
17	5.707	403	34.8	0.0747268	5	0.569741	1.2	1	5.707	402.8	5.5	0.0588461
18	5.771	418	36.1	0.0707305	10	1.25141	2.6	2.3	5.771	416.7	10	0.0705525
19	5.86	439	37.9	0.0255865	3	0.105138	0.2	0.2	5.86	439.2	3.3	0.0177768
20	5.936	458	39.6	0.576993	6	5.62334	11.8	10.2	5.936	459.7	6	0.531074
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.036	483	41.7	0.468482	6	4.38839	9.2	7.9	6.036	482.8	6	0.412626
22	6.12	504	43.6	0.347292	8	4.43221	9.3	8	6.12	503.9	8.2	0.305648
23	6.148	511	44.2	0.205611	3	0.339908	0.7	0.6	6.148	512.4	3.4	0.0569043
24	6.24	534	46.2	0.0147025	5	0.106356	0.2	0.2	6.24	533.7	4.5	0.0133103
25	6.28	544	47	0.191356	2	0.807499	1.7	1.5	6.28	544.2	2.4	0.189676
26	6.364	565	48.8	0.0219311	14	0.739767	1.5	1.3	6.364	577	14.5	0.0288775
27	6.44	584	50.5	0.300962	2	1.13455	2.4	2.1	6.44	583.9	2.3	0.280532
28	6.484	595	51.4	0.0245441	2	0.0430307	0.1	0.1	6.484	596.1	1.8	0.0134819
29	6.54	609	52.6	0.0315681	3	0.151565	0.3	0.3	6.54	609.2	3	0.028263
30	8.823	1034	89.4	0.113272	18	3.52495	7.4	6.4	8.823	1025.5	18	0.110488

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	57	4.9	0.180764	10	3.05075	10.5	8.2	3.5	58	9.7	0.178011
2	4.55	207	17.9	0.241756	9	3.55603	12.2	9.6	4.55	209.1	8.6	0.232383
3	5.2	289	25	0.333197	8	4.33615	14.9	11.7	5.2	290	7.5	0.325439
4	5.85	442	38.2	0.555812	4	3.59816	12.4	9.7	5.85	441.6	3.5	0.57425
5	6.55	613	53	0.419143	4	3.12626	10.8	8.4	6.55	612.6	4.5	0.394359
6	6.85	653	56.4	0.192853	6	1.85974	6.4	5	6.85	653.4	6.2	0.169064
7	7.35	744	64.3	0.387418	5	3.26398	11.2	8.8	7.35	743.1	4.8	0.380892
8	8.15	904	78.1	0.0541742	10	0.976079	3.4	2.6	8.15	902.4	10.4	0.0531949
9	8.45	965	83.4	0.0746374	7	0.868061	3	2.3	8.45	966	6.7	0.0727453
10	8.65	1006	86.9	0.0736216	5	0.684332	2.4	1.8	8.65	1005.1	5.4	0.0716699
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1123	97.1	0.263494	8	3.75725	12.9	10.1	9.3	1121.1	8.3	0.256512

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP CO MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 125

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 16.400 4.550 9.500 5.200 12.100 5.850 11.900 6.550 10.200 6.850 5.700 7.350 10.100 8.150 3.500 8.450 3.600 8.650 2.900 9.300 14.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
4.133 17.200 4.598 3.100 4.711 4.800 4.792 1.200 4.930 8.900 5.059 0.400 5.148 0.400 5.202 0.800 5.244 9.400 5.311 7.000 5.395 1.600 5.450 0.500 5.554 0.400 5.630 1.000 5.684 2.400 5.747 2.100 5.840 0.300 5.918 11.700 6.029 6.000 6.099 2.100 6.128 4.800 6.161 1.900 6.243 0.100 6.297 1.700 6.379 1.500 6.457 2.500 6.507 0.200 6.567 0.300 8.814 5.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 29 Return to Lane Navigator
3.500 2.700 4.146 10.300 4.439 0.100 4.550 4.000 4.743 3.100 4.823 0.500 4.943 4.400 5.023 0.100 5.104 1.000 5.200 2.800 5.265 7.100 5.404 1.300 5.451 0.300 5.490 0.100 5.573 0.100 5.681 1.400 5.720 0.600 5.781 0.500 5.850 4.400 5.939 8.900 6.049 5.700 6.155 2.300 6.172 2.500 6.317 1.000 6.495 2.000 6.550 4.400 6.850 2.300 7.350 5.900 8.150 4.000 8.450 1.200 8.650 1.400 8.812 3.700 9.300 9.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 33 Return to Lane Navigator
3.500 16.400 4.550 8.100 5.200 11.600 5.850 11.000 6.550 9.700 6.850 5.100 7.350 9.700 8.150 3.600 8.450 3.400 8.650 2.900 9.300 18.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
4.159 14.100 4.342 2.500 4.476 0.100 4.617 0.200 4.673 1.900 4.800 3.800 5.006 7.100 5.156 1.100 5.224 1.700 5.292 10.700 5.348 4.400 5.425 1.600 5.472 0.500 5.519 0.100 5.587 0.500 5.655 1.200 5.707 1.200 5.771 2.600 5.860 0.200 5.936 11.800 6.036 9.200 6.120 9.300 6.148 0.700 6.240 0.200 6.280 1.700 6.364 1.500 6.440 2.400 6.484 0.100 6.540 0.300 8.823 7.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 30 Return to Lane Navigator
3.500 10.500 4.550 12.200 5.200 14.900 5.850 12.400 6.550 10.800 6.850 6.400 7.350 11.200 8.150 3.400 8.450 3.000 8.650 2.400 9.300 12.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.