IX IUPAC Symposium on Mycotoxins and Phycotoxins
FINAL PROGRAMME

Monday, 27 May

8.30 - 10.00 Registration

10.00 - 11.00 - Opening ceremony and Welcome
Live in the Aula Pocchiari
Closed-circuit television in the Aula Bovet

Chairperson: Marina Miraglia

• Minister for Health
• Prof. Aurelia Sargentini (Director of the Istituto Superiore di Sanita)
• Prof. Pieter Steyn (IUPAC Representative)
• Prof. Angelo Stacchini (Director of the Food Laboratory - Istituto Superiore di Sanita)
• Prof. Romano Marabelli (Director of the Department of Food and Nutrition and Veterinary Medicine Services - Ministry of Health)

11.00 - 11.45 - KEYNOTE LECTURE
Mycotoxins and Phycotoxins

Chairperson: M. Miraglia

11.45 - 12.10 Coffee Break

MYCOTOXINS

Aula Pocchiari : SESSION A
Chairperson: C. P. Wild

Plenary Lecture

12.10 - 12.40
PLM1 - mycotoxins: OCCURRENCE IN FOOD AND BIOLOGICAL FLUIDS
J. D. Groopman - Johns Hopkins University (USA)

Contributed Papers

12.40 - 12.55
OM1 - Occurrence of fumonisins in foods in Germany
E. Usleber, C. Schlichtherle, M. Lakner and E. Martlbauer

12.55 - 13.10
OM2 - Evaluation of incidence of mycotoxins in Italy: a comparison of three different methodological approaches
M. Miraglia, C. Brera, R. Onori, S. Corneli, M. Colatosti, E. Cava, D. Ippoliti, M. Quaglia

13.10 - 13.20 - Discussion

13.20 - 14.30 - Lunch

14.30 - 15.30 Aula marotta: Poster Session

Aula Pocchiari
Chairpersons: D. Park and C. Brera

Plenary Lecture

Contributed Papers

16.00 - 16.15
OM3 - Occurrence of Fusarium mycotoxins in cereals in Russia
V. A. Tutelyan

16.15 - 16.30
OM4 - Ochratoxin A (OTA) in table wine and grape juice
B. Zimmerli, R. Dick

16.30 - 16.45
OM5 - Occurrence of aflatoxins B1, B2, G1, G2, in imported goods in Greece during 1995
E. Apergi, J.P. Gardikis, V.J. Panagiotopoulou

16.45 - 17.00 - Discussion

17.00 - 17.20 - Coffee Break

17.20 - 17.35
OM6 - Occurrence of fumonisins in corn feedstuffs intended for pig consumption in Bretain (France)
I. Dragoni, L. Piantanida, Y. Tyrilli, A. Visconti, M. Pascale

17.35 - 17.50
OM7 - Aflatoxin contamination in different types of peanut kernels as defined by the Brazilian Official Classification
A. H.O. Stefanovitz, H. Fonseca, M.A.C. Domingues, E.M. Gloria

17.50 - 18.05
OM8 - Mycotoxin contamination of maize grown in Australia, the Philippines and Vietnam
W.L. Bryden, G. Ravindran, M.T. Amba, R.J. Gill, L.W. Burgess

18.05 - 18.20 - Discussion

Aula Bovet: Session B
Chairperson: P.G. Thiel

17.30 - 17.45
OM9 - The detection and measurement of aflatoxin B1 conjugates in humans in Natal, South Africa
M.F. Dutton, S. Myeni, D. Early, A. Chuturgoon, S. Bux

17.45 - 18.00
OM10 - Toxicity and occurrence of Fusarium species and mycotoxins in late harvested and overwintered grain from Norway, 1993.
W. Langseth, Per-Erik Clasen, B. Kosiak, M. Torp, M. Gareis

18.00 - 18.10 - Discussion

20.00 - Welcome Drink

Phycotoxins

Aula Bovet
Chairperson: M. L. Fernandez

Plenary Lecture

12.10 - 12.40
PLP1 - OCCURRENCE AND MONITORING OF HARMFUL ALGAE IN THE MARINE ENVIRONMENT
O. Lindahl - Kristineberg Marine research Station (Sweden)

12.40 - 12.55
OP1 - Biotoxins on the Pacific Coast of Mexico
J.L. Ochoa, A. Cruz-Villacorta, E. Nunez-Vazquez, A. Sanchez-Paz, A.P. Sierra-Beltran

12.55 - 13.10
OP2 - Immunochemical Survey of microcystins in environmental water in various countries
Y. Ueno, S. Nagata, M. Sutajjit, D. Mebs, V. Vasconcelos

13.10 - 13.20 - Discussion

13.20 - 14.30 - Lunch

14.30 - 15.30 Aula marotta: Poster Session

Aula Bovet
Chairpersons: J.M. Fremy and Y. Ueno

Plenary Lecture

15.30 - 16.00
PLP2 - OCCURRENCE OF AQUATIC BIOTOXINS IN SEAFOODS
T. Yasumoto - Tohoku University (Japan)

16.00 - 16.20 Coffee Break

Contributed Papers

16.20 - 16.35
OP3 - Mussel food selection and DSP contamination
L. Sidari, P. Nichetto, A. Tubaro, G. Honsell, R. Della Loggia

16.35 - 16.50
OP4 - Diarrhetic shellfish poisoning (DSP) toxins in Dinophysis Fortii samples from the Adriatic sea
R. Draisci, L. Giannetti, L. Lucentini, P. Boria, R. Poletti, L. Boni, E. Funari

16.50 - 17.00 - Discussion

17.00 - 17.20 - Coffee Break

20.00 - Welcome drink

21.00 - A MUSICAL EVENING

Tuesday, 28 May
Live in the Aula Pocchiari
Closed circuit television in the Aula Bovet

9.00 - 9.45 - KEYNOTE LECTURE
Mycotoxins and Phycotoxins

Chairperson: H.P. van Egmond

K2 - Food safety : mycotoxins and phycotoxins in Perspective T. Kuiper Goodman - Health CANADA (Canada)

MYCOTOXINS

Aula Pocchiari
Chairperson: H.P. van Egmond

Plenary Lectures

9.45 - 10.15
PLM3 - TOXICITY AND METABOLISM
J. L. Richard - USDA/Agriculture RESEARCH SERVICE (USA)

10.15 - 10.45
PLM4 - mycotoxins: MECHANISMS OF ACTION AT THE MOLECULAR LEVEL
J. Fink-Gremmels - Utrecht University (The Netherlands)

10.45 - 11.00 - Coffee Break

Chairpersons: J. D. Groopman and T. Kuiper-Goodman
Contributed Papers

11.00 - 11.15
OM11 - Hepatocellular uptake of ochratoxin A is carrier-mediated and occurs via the cloned organic anion transporting polypeptide OATP
M. Kontaxi, U. Eckhardt, B. Hagenbuch, B. Stieger, P.J. Meier, E. Petzinger

11.15 - 11.30
om12 - Ochratoxin A occurrence in serum, placenta and funiculum samples of Italian people
M. Miraglia, C. Brera, S. Corneli, M. Colatosti, E. Cava, G. Montanino, E. Miraglia, F. Ventola, F. Ciaraldi, V. Miele

11.30 - 11.45
OM13 - Aflatoxin B1 in urine of humans with chronic B and C hepatitis and cirrhosis in Mexico
M.T. Alvarez , M. Carvajal , M. Lisker-Melman

11.45 - 12.00
OM14 - Toxicokinetics of fumonisins B1, and B2: comparative studies in rats and non-human primates
G.S. Shephard, P.G. Thiel, E.W. Sydenham, M.E. Savard, P.W. Bakkes and R. Vleggaar

12.00 - 12.20 - Discussion

12.20 - 12.35
OM15 - Co-carcinogenicity study of fumonisin B1 and N-methyl-N-benzylnitrosamine in relation to cancer of the oesophagus in rats
C.P. Wild, L. Garren, M. Castegnaro, H. Ohgaki , D.Galendo, J.D. Miller

12.35 - 12.50
OM16 - Improved method for sphinganine/sphingosine determination in human urine as a biomarker of fumonisin exposure
M. Solfrizzo, G. Avantaggiato, A. Visconti

12.50 - 13.05
OM17 - Metabolic aspects of aflatoxin toxicity with particular reference to the expression of potentially protective pathways
G.E. Neal, M.M. Manson

13.05 - 13.20 Discussion

13.20 - 14.30 - Lunch

14.30 - 15.30 Aula marotta: Poster session

15.30-18.00 Aula Pocchiari
Sampling Workshop
Chairpersons: D. Park and R. Coker

S1 - UK Sampling Plan for aflatoxins in food
J. Gilbert

S2 - The Dutch code of practice on peanuts in perspective
W.J. de Koe

S3 - Control of mycotoxins in the US: Sampling Plans
A.E. Pohland

S4 - The Development of Sampling Plans for the determination of aflatoxins in animal feedingstuffs
R. COKER, M. NAGLER, P. DEFIZE, G. DERKSEN, J. HOOGLAND, H. BUCHHOLZ

S5 - Mycotoxins: Sampling Plans
H. ROSNER

S6 - Methods of sampling and analysis for mycotoxin detection in food and feed
C. CANET, J. PAAKKANEN

PHYCOTOXINS

Aula Bovet
Chairpersons: T. Yasumoto and L. Toti

Plenary Lectures

9.45-10.15
PLP3 - toxicity and metabolism of phycotoxins
L. Edebo - Goteborg University (Sweden)

10.15 - 10.45
PLP4 - toxins produced by cyanobacteria
K. Sivonen - Helsinki University (Finland)

10.45 - 11.00 - Coffee Break

Contributed Papers

11.00 - 11.15
OP5 - Implication of okadaic acid on DNA adduct formation and erod activity
A. Pfohl-Leskowicz, V. Fessard, M. Lafaurie, Y. Gross , Puiseux-Dao

11.15 - 11.30
OP6 - Okadaic acid reorganizes the actin cytoskeleton in intestinal cultured cells
C. Fiorentini, P. Matarrese, A. Fattorossi, G. Donelli

11.30 - 11.45
OP7 - Unexplained toxicity in molluscs found in the course of phytoplankton monitoring
C. Marcaillou-Le Baut, Z. Amzil

11.45 - 12.00 Discussion

12.00 - 12.15
OP8 - Cytotoxic activity of new diarrhoetic shellfish toxins isolated from Italian mussels
R. Draisci, L. Lucentini, L. Giannetti, P. Boria, A. Stammati, F. Nardelli, F. Zampaglioni, F. Zucco

12.15 - 12.30
OP9 - Biosensor application for the detection of okadaic acid in mussels Al. Stacchini, L. Cozzi, L. Toti, F. Botre, F. Mazzei 12.30 - 12.45 OP10 - Immunoaffinity chromatography as a tool for the analysis of paralytic shellfish poisoning toxins
R. Dietrich, C. Burk, E. Usleber, E. Martlbauer, M.V. Laycock

12.45 - 13.20 - Discussion

13.20 - 14.30 - Lunch

14.30 - 15.30 Aula marotta: Poster Session

Aula Bovet
Chairpersons: O. Lindahl and R. Draisci

Plenary Lecture

15.30 - 16.00
PLP5 - management STRATEGIES for phycotoxin control
H. Ravn - IOC/UNESCO (France)

16.00 - 16.15 - Coffee Break

Contributed Papers

16.15 - 16.30
OP11 - Identification of marine polyether compounds by tandem mass spectrometry
H. Naoki. T. Fujita, M. Satake, T. Yasumoto

16.30 - 16.45
OP12 - Determination of microcystins in aqueous and cyanobacterial samples using liquid micro-chromatography and capillary electrophoresis
C. Rivasseau, N. Boua‹cha, M.C. Hennion, P. Sandra

16.45 - 17.00 - Discussion

21.00 - A MUSICAL EVENING

Wednesday, 29 May

MYCOTOXINS

Aula Pocchiari
Chairpersons: C. Canet and J.M. Fremy

Plenary Lecture

9.00 - 9.30
PlM5 - mycotoxins: LIMITS AND EFFECTS ON TRADE
H.Rosner - Federal Institute for Health Protection of Consumers and veterinary medicine (Germany)

Contributed Papers

9.30 - 9.45
OM18 - Fusarium mycotoxin beauvericin: optimisation of analytical methodology and natural occurrence in maize ears in Austria
R. Krska, R. Schuhmacher, M. Grasserbauer, M. Lemmens, P. Ruckenbauer, A. Alder, H. Lew

9.45 - 10.00
OM19 - Use of Polymerase Chain Reaction for the detection of Fusaria
M.L Niessen, R.F. Vogel

10.00 - 10.15
OM20 - Use of Capillary Electrophoresis for the separation of ochratoxin A and its albumin conjugate in serum samples with fluorescence detector
C. Desiderio, S. Fanali

10.15 - 10.30 Discussion

10.30 - 10.50 - Coffee Break

10.50 - 11.05 OM21 - HACCP and mycotoxins: what is the point of interaction?
C. Brera, M. Miraglia, R. Onori

11.05 - 11.20
OM22 - Proficiency testings for aflatoxin M1 monitoring programme: a five year experience in France
S. Dragacci, J.M. Fremy

11.20 - 11.35
OM23 - Use of mass spectrometry to solve problems of analysis concerning mycotoxins
F.Tateo, M. Miraglia, M. Bononi

11.35 - 11.50
OM24 - Rapid detection of ochratoxin A in wheat by flow-through membrane-based enzyme immunoassay
S. De Saeger, C. Van Peteghem

11.50 - 12.05
OM25 - Development of metabolically competent in vitro models for food safety evaluation
C.W. Lewis, J.E. Smith, J.G. Anderson

12.05 - 12.30 Discussion

13.00 - 14.30 - Lunch

Afternoon Session
Aula Pocchiari: Italian Open Forum on Mycotoxins

PHYCOTOXINS

Aula Bovet
Chairpersons: H. Ravn and L. Croci

Plenary lectures

9.00 - 9.30
PlP6 - Limits and effects on trade
M. L. Fernandez - health ministry - estacion maritima (Spain)

9.30 - 10.00
PLP7 - phycotoxins - a review of chemical and biological methods
D. J. Hannah - Institute of Environmental Science AND Research (New Zealand)

10.00 - 10.20 - Coffee Break

Contributed Papers

10.20 - 10.35
OP13 - Photocatalytic decomposition of microcystins in water
S. Stockenstrom, D. de Villiers, W. J. Engelbrecht, G.S. Shephard, E.W. Sydenham, P.G. Thiel, G.F.S. Wessels

10.35 - 10.50
OP14 - Removal of microcystins in lake water by water treatment process
G. Chen, Chuan-wei Chen, Shun-Zhang Yu, Y. Ueno

10.50 - 11.05
OP15 - A new constituent of diarrhetic shellfish toxins in Irish mussels
K.J. James, E.P.Carmody, S.S. Kelly, R. Draisci, L. Lucentini, L. Giannetti, A. Stacchini

11.05- 11.20
OP16 - Sensitive HPLC-Fluorometric and HPLC-MS determination of Diarrhetic Shellfish Poisoning (dsp) - toxins as 4-Bromomethyl-7-methoxycoumarin esters
J.L. Shen, C. Hummert, B. Luckas

11.20 - 11.40 Discussion

11.40 - 12.40 Aula Marotta : Poster Session

13.00 - 14.30 - Lunch

Thursday, 30 May
Live in the Aula Pocchiari
Closed circuit television in the Aula Bovet

9.00 - 9.45 - KEYNOTE LECTURE
Mycotoxins and phycotoxins

Chairperson : J Gilbert

K3 - OVERVIEW of international mycotoxin and phycotoxin programs A. E. Pohland - US Food and Drug Administration (USA)

MYCOTOXINS

Aula Pocchiari
Chairpersons : J Gilbert and M. Jemmali

Plenary Lecture

9.45 - 10.15
PLM6 - microbial Ecology and mycotoxin formation
J. Smith, J. Lacey, R. Cuero, N. Ramakrishna, N. Gqaleni - University of Strathclyde (UK)

10.15 - 10.30 - Coffee Break

Contributed Papers

10.30 - 10.45
OM26 - Scientific Co-operation task on aflatoxins: the co-ordinators view
A. E. Gleadle, E.M. Mortby, A. Hatch, R. Burt

10.45 - 11.00
OM27 - The effect of a commercial herbicide on aflatoxin and lipid synthesis, in Aspergillus parasiticus (N1X)
A. A. Chuturgoon, R. Punchoo, S. Bux, M. F. Dutton

11.00 - 11.15
OM28 - Interactive effects of zinc and associated mycoflora in toxigenic Fusarium DNA and Aspergillus in relation to growth and toxins production
R.G. Cuero, T. Ouellet

11.15 - 11.30
OM29 - Freeze-dried mixed cultures of microfungi for proficiency tests
M. Olsen, K. Andersson, A. Rosengren

11.30 - 11.50 Discussion

11.50 - 12.05
OM30 - Detection of aflatoxigenic moulds in grains by the polymerase chain reaction
R. Shapira, N. Paster, O. Eyal, M. Menasherov, A. Matt, R. Salomon

12.05 - 12.20
OM31 - Regulation of sterigmatocystin biosynthesis during sporulation of Aspergillus nidulans
D. Guzman de Pena, J. Aguirre, J. Ruiz Herrera

12.20 - 12.35
OM32 - Aflatoxin production related with CO2/O2 rate in Aspergillus Parasiticus
E. Finotti , C. Paolino, A. Ricelli, R. Mercantini

12.35- 12.50
OM33 - Taxonomy and chemotaxonomy of mycotoxin producers: how consistent is mycotoxin production in a species?
J.C. Frisvad, T. O. Larsen, J. Smedsgaard, M.S. Nielsen, P.V. Nielsen, F. Lund, A. Svendsen, U. Thrane , O. Filtenborg

12.50 - 13.10 Discussion

13.10 - 14.30 - Lunch

14.30 - 15.30 Aula Marotta: Poster Session
Aula Pocchiari
Chairpersons: G. Piva and A. Visconti

Plenary Lecture

15.30 - 16.00
PLM7 - MOLECULAR EPIDEMIOLOGY OF MYCOTOXIN - RELATED HUMAN DISEASE
C.P. Wild - UNIVERSITY OF LEEDS (UK)

16.00 -16.20 - Coffee Break

16.20 - 16.35
OM34 - Microbiological degradation of mycotoxins
E.M. Horvath, J. Binder, N. Ellend, J. Heidegger, E. Schaffer

16.35- 16.50
OM35 - Development of a technique for the prediction/alleviation of conditions leading to mould growth in houses
J.A. Clarke, J.E. Smith, J.G. Anderson, R.C. McLean, N.J. Rowan, N.J. Kelly, C. Johnstone

16.50 - 17.05
OM36 - Moniliformin accumulation in wheat kernels inoculated with Fusarium avenaceum
P.Golinski, I. Lasocka, M. Kostecki, H. Wisniewska, J. Chelkowski, Z. Kaczmarek
17.05 - 17.20
OM37 - Ochratoxin A in ten rice cultivars after inoculation of Aspergillus ochraceus
K. Axberg, G. Jansson, L.L. Ilag, T.W. Mew, K. Hult

17.20 - 17.40 - discussion

CLOSING SESSION
17.40 - 18.00
H.P.van Egmond
P.G. Thiel
M. Miraglia

Friday, 31 May

Aula Pocchiari
FAO/IUPAC Workshop
Chairperson: C. Canet

Morning Session: 9.30 - 12.30

Introductory remarks:
Introduction to the workshop on Mycotoxins: FAO activities
C. Canet - FAO

Mycotoxin prevention and control: increasing awareness
L.A. Esteves - NAPHIRE, The Philippines

Mycotoxin control in Latin America: Networking
O. Sanchez Regueiro - Instituto de nutricion e higiene, Cuba

Mycotoxin prevention and control: experience in Africa
E. Wilbald - Government Chemist Laboratory, Tanzania

Afternoon Session: 14.00 - 17.30

Check sample programmes: experience in Latin America
S. Resnik - University of Buenos Aires, Argentina

Mycotoxin reference standards and kits
O. Sanchez Regueiro - Instituto de nutricion e higiene, Cuba

Training in Mycotoxin analysis
B. Doko - University of Montpellier

Open Discussion

Conclusions and Recommendations

Aula Bovet
SM&T Session
Chairperson: A. Boenke and J. Gilbert

9.00- 9.20
SMT1 - The Standards, Measurements and Testing Programme (SM&T) - The European Support to Standardisation, Measurements and Testing Projects
A. Boenke

9.20 - 10.00 - Two examples of typical projects supported by the SM&T:

9.20 - 9.40
SMT2 - Improvements in the Determination of Fumonisins (FB1 and FB2) in Maize and Maize Based Feeds
A. Visconti

9.40 - 10.00
SMT3 - The Development of Reference Materials for Paralytic Shellfish Poisons (PSP) - Coordination, Management and other Relevant Project Aspects
H.P. van Egmond

10.00 - 10.30
SMT4 - How to submit a Proposal to a call for proposal within the EC, Standards, Measurements and Testing Programme (SM&T)
A. Boenke

10.30 - 11.00 Discussion

11.00 - 11.20 - Coffee Break

11.20 - 12.20
Final proposers forum at the stand of the european commission, SM&T Programme

Last Minute Announcement

the Organising Committee is pleased to inform participants that apart from Monday (8.30 - 10.00), it will also be possible to register on Sunday afternoon from 4 pm until 7pm at the ISTITUTO SUPERIORE DI SANITA - VIALE REGINA ELENA, 299. We would advise registering on Sunday in order to avoid delays in registration on Monday.

FOR FURTHER INFORMATION CONTACT:

SYMPOSIUM SECRETARIAT
Secretariat for Cultural Affairs
Istituto Superiore di Sanita
Viale Regina Elena, 299
00161 - Rome, Italy
Tel: +39-6-49902611 or 49902292
Fax: +39-6-4440235

Local Scientific Coordinators:

Dr. Marina Miraglia, Dr Carlo Brera
Istituto Superiore di Sanita
Laboratorio Alimenti
Viale Regina Elena, 299 - 00161 Rome, Italy
Tel: +39-6-49902367 or 49902377
Fax: +39-6-49902377 or 4451767
E-Mail: miraglia@istan.interbusiness.it

TRAVEL AGENCY

Servizi Internazionali S.r.l
Viale Pasteur, 5
00144 Rome, Italy

Contact persons:

Mr Paolo Missiaja, Ms Stefania Mura
Tel: +39-6-5912284
Fax: +39-6-5916750