Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Silk Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus vivanus
FDA Seafood List Common Name: Silk Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940822gel02
Unique Web Code: ssh03Srr
Tissue Aliquot Code: lutjviva H94-004 #05a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559311.20.078664770.84203411.394.5593.76.60.0716846
25.219523.50.069276360.6299988.46.85.2195.25.60.0640069
35.8531037.30.12327251.0556914.111.35.85310.14.90.120961
46.5543452.20.094226850.825092118.96.55433.950.0930392
57.3553464.30.030410760.2875193.83.17.355355.50.0293503
68.1565578.80.087769681.1397515.212.28.15656.17.70.0837779
78.4572186.80.093047671.228816.413.28.45721.67.50.0927721
88.6576692.20.077832450.6697358.97.28.65766.24.90.076808
99.380997.40.088430350.80593210.88.79.3808.65.30.0853657
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.132222.60.026242270.2892870.90.74.13222.56.60.0248146
24.352576.90.24531262.5858186.24.35257.560.242381
34.70411313.60.019187950.1516820.50.44.704113.44.50.0188923
44.829133160.014103670.1704710.50.44.829132.76.80.014129
54.99315919.10.33239163.2245107.74.993159.160.304445
65.33521525.90.17124331.022223.22.45.335215.23.50.16596
75.68827533.10.10982871.216673.82.95.688274.46.50.104992
85.85330336.50.087870950.7145072.21.75.853302.95.10.0786304
96.02133440.20.65118633.52579118.46.021334.33.10.631622
106.11335142.20.34710842.224676.95.36.113350.93.70.335899
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.236744.20.25793952.205716.95.26.2366.14.80.260422
126.27138045.70.017809720.06750550.20.26.271380.72.40.0156494
136.34739447.40.052614730.31159510.76.347393.23.20.0547042
146.48241950.40.173641.245783.936.482418.84.40.161342
156.62744153.10.6065743.9724912.49.56.627441.23.80.595527
166.73245454.60.39105831.863265.84.46.732453.62.60.396774
176.81346455.80.18834941.455034.53.56.813464.24.50.184287
187.24951862.30.15814741.115713.52.77.249518.14.20.15022
197.56856467.90.0265758120.5687631.81.47.568562.911.60.0277396
207.65957869.60.057450150.4310711.317.659577.94.90.0498816
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.83560572.80.34118952.592678.16.27.835605.24.50.322982
229.3380997.40.0614505111.201763.72.99.33804.611.20.060764
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558410.10.051128670.5468178.26.54.5585.36.50.0473619
25.218922.80.075515840.4811377.25.75.2188.73.80.0717022
35.8529535.50.10502150.908813.710.75.85294.95.10.100813
46.5542951.70.086770760.81332912.39.66.554285.50.0827687
57.3552763.50.023833760.2452513.72.97.35527.36.10.0228135
68.1565278.60.083962181.062111612.58.156527.60.0786134
78.45714860.085832681.178317.813.98.45714.18.10.0824729
88.6576091.60.066225160.6596629.97.88.65760.65.80.0641991
99.3805970.091399450.74205611.28.89.3805.34.70.0890406
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.104222.70.011730260.1154180.40.44.10420.96.10.0106123
24.321566.80.16218551.5769665.24.32156.85.40.165215
34.559211.10.055394360.5580472.11.84.559360.0520744
44.70911714.10.0127083100.1930290.70.64.709116.810.10.0108265
54.84313816.70.010079550.08763890.30.34.8431385.40.0090982
65.015165200.10356771.29434.94.35.015165.87.40.0983453
75.219423.50.046167130.8576923.32.85.218913.50.0358659
85.357223270.083475840.52675921.75.357223.23.80.0785208
95.65527833.60.0378958171.183564.53.95.655273.7170.0392984
105.85314380.052243770.6167952.325.85313.97.10.0490781
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.06534942.20.32903263.2149412.210.76.065348.35.80.31475
126.15136343.90.19411251.550335.95.16.1513634.60.190595
136.23737745.60.16377951.468335.64.96.237376.24.90.167493
146.31739047.20.011729920.03586670.10.16.3173912.10.0095984
156.40340448.90.020714740.13970.50.56.403403.43.70.0214046
166.5542851.80.11025161.03143.93.46.55427.75.60.10485
176.69344754.10.48589643.5715913.611.86.693447.74.10.489659
186.78445955.50.28544141.80186.966.784458.93.80.269688
196.85946956.70.14252161.372225.24.56.8594695.60.138595
207.28252563.50.069176660.6553152.52.27.282523.65.70.0649448
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.3553464.60.021826340.1496010.60.57.35535.43.90.0216865
227.57456768.60.013124250.1118950.40.47.574567.94.70.0133172
237.67558270.40.023834940.1737640.70.67.675582.840.0244445
247.84560773.40.21666862.010777.66.77.845607.25.70.199761
258.1565278.80.023896440.1274340.50.48.15651.53.80.0189521
268.4572587.70.040316670.51440821.78.45725.87.30.0399112
278.6576692.60.041281850.3323251.31.18.65765.94.60.0409074
289.380797.60.080043681.013313.93.49.3805.38.20.0696357
PeakCounter: 28
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.149303.60.022911870.27852510.84.14929.37.10.0220242
24.358637.60.26585562.714489.47.54.35862.75.80.264829
34.7312214.70.015330450.1229920.40.34.73120.44.60.0149871
44.85141170.017916870.1956120.70.54.85142.76.50.0168927
55.03917120.60.19936362.058117.15.75.039171.56.20.186062
65.36622627.20.16433330.9627773.32.75.366225.93.40.159699
75.68728334.10.13212471.658535.84.65.687282.67.30.127948
85.88531838.30.049560460.4941121.71.45.885319.55.90.046927
96.06134841.90.49261932.9709110.38.26.061347.73.40.487281
106.13736143.40.29251541.959736.85.46.137360.540.27617
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.213374450.24361541.931896.75.36.213373.74.50.244124
126.27838546.30.00807920.03194030.10.16.278387.61.90.0093384
136.36399480.052595230.3181571.10.96.36399.23.40.0533119
146.52442751.40.15313151.12963.93.16.524425.84.50.141202
156.67244753.80.55209343.5453412.39.86.672447.53.60.551657
166.76745955.20.36587931.654635.74.66.7674592.70.352188
176.85347056.60.16250841.210424.23.36.853469.44.30.159834
187.28752563.20.15671641.1597743.27.287524.54.50.146209
197.59957068.60.016502760.1760510.60.57.599569.56.20.0159487
207.66658069.80.050840340.3594621.217.666580.440.0508111
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.81460272.40.30215552.512948.76.97.814602.24.80.294171
229.277806970.0557196141.378874.83.89.27780114.50.0537799
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559311.20.079654480.95783711.38.94.55947.60.0714088
25.219723.70.093161140.632467.45.95.2196.93.90.0915007
35.8530837.10.12939951.0254812.19.65.85308.64.60.126233
46.5543352.10.10070761.0315112.19.66.55432.560.0973281
57.3553063.80.041139360.4025464.73.87.35529.860.0381637
68.1565078.20.09105171.1013912.910.38.15649.470.0890698
78.4571385.80.098207891.4638817.213.68.45712.88.50.0966884
88.6576291.70.11164540.8590510.188.65761.64.50.107775
99.3806970.1024161.0344712.29.69.3805.26.20.0939768
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic

 
Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 99
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 28 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse   |   RFE Tutorial   |   Seafoods   |   CFSAN


Last updated on 2003-FEB-27 by bjt/frf/nln