RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19970423gel05
Unique Web Code: rsg02Srr
Tissue Aliquot Code: lutjcamp D97-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	100	4.5	0.304845	26	13.5628	21.5	15.6	3.5	104.1	26.2	0.292334
2	4.55	425	19.2	0.270581	10	4.69616	7.4	5.4	4.55	426.2	10.5	0.25261
3	5.2	609	27.5	0.344332	9	5.3424	8.5	6.1	5.2	609.4	8.8	0.342007
4	5.85	918	41.4	0.491629	7	6.4312	10.2	7.4	5.85	918.6	7.2	0.501912
5	6.55	1252	56.4	0.332078	9	4.86735	7.7	5.6	6.55	1251.5	8.8	0.313289
6	6.85	1335	60.2	0.236977	10	3.74655	5.9	4.3	6.85	1334.5	9.6	0.221335
7	7.35	1511	68.1	0.304989	11	5.63097	8.9	6.5	7.35	1508.3	10.6	0.299937
8	8.15	1796	81	0.131308	17	3.92052	6.2	4.5	8.15	1794.5	17.5	0.126405
9	8.45	1910	86.1	0.203616	11	3.93094	6.2	4.5	8.45	1909.3	11.2	0.198184
10	8.65	1976	89.1	0.185657	10	3.24061	5.1	3.7	8.65	1975.2	10.2	0.179269
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2193	98.9	0.359705	14	7.73622	12.3	8.9	9.3	2189.3	13.6	0.321036

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.791	191	8.6	0.236729	13	5.68075	9.6	6.3	3.791	191.2	13.4	0.239586
2	4.307	348	15.7	0.508159	10	9.89593	16.7	10.9	4.307	347.2	10.5	0.534177
3	5.03	556	25.1	0.14121	7	1.66192	2.8	1.8	5.03	556.3	6.7	0.140158
4	5.302	653	29.5	0.0975761	17	2.70099	4.6	3	5.302	648.4	16.5	0.0921024
5	5.362	682	30.8	0.567878	6	5.30609	8.9	5.9	5.362	682.8	5.6	0.535045
6	5.678	835	37.7	0.457658	9	7.3333	12.4	8.1	5.678	835.6	8.8	0.472559
7	5.867	926	41.8	0.119045	7	1.25861	2.1	1.4	5.867	926.1	6.6	0.10721
8	5.99	984	44.4	0.588874	7	6.93377	11.7	7.7	5.99	985.6	6.7	0.581641
9	6.079	1026	46.3	0.383025	6	4.03162	6.8	4.5	6.079	1025.6	6.2	0.365573
10	6.162	1065	48	0.154643	12	3.16042	5.3	3.5	6.162	1061	12.2	0.146748
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.508	1228	55.4	0.206295	5	1.72362	2.9	1.9	6.508	1227.7	4.7	0.206838
12	6.561	1251	56.4	0.0996154	5	0.824298	1.4	0.9	6.561	1249.3	4.6	0.100591
13	6.629	1270	57.3	0.362798	10	5.75418	9.7	6.4	6.629	1272.3	10.1	0.320827
14	7.237	1469	66.3	0.106491	5	0.915745	1.5	1	7.237	1469	4.8	0.107142
15	7.782	1664	75.1	0.180465	7	2.14184	3.6	2.4	7.782	1664.2	6.8	0.178239

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	94	4.3	0.260077	34	14.4171	22.9	17.5	3.5	100.7	33.7	0.24117
2	4.55	408	18.5	0.258364	10	4.2235	6.7	5.1	4.55	408.5	9.8	0.242108
3	5.2	587	26.6	0.343848	9	5.34196	8.5	6.5	5.2	587.6	8.9	0.340494
4	5.85	907	41.1	0.541862	7	6.74737	10.7	8.2	5.85	908.5	6.9	0.555036
5	6.55	1233	55.9	0.351225	9	4.97869	7.9	6	6.55	1232.6	8.7	0.322244
6	6.85	1317	59.7	0.253418	10	4.06879	6.4	4.9	6.85	1316.8	9.6	0.239085
7	7.35	1496	67.8	0.321867	10	5.72163	9.1	6.9	7.35	1493.9	10.5	0.307629
8	8.15	1785	80.9	0.114156	19	3.66805	5.8	4.5	8.15	1784.7	19.3	0.107413
9	8.45	1899	86.1	0.19161	11	3.68562	5.8	4.5	8.45	1898.8	11.1	0.186947
10	8.65	1965	89.1	0.165112	10	2.77701	4.4	3.4	8.65	1963.8	9.6	0.162817
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2182	98.9	0.348312	14	7.46389	11.8	9.1	9.3	2178.2	13.6	0.309118

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	101	4.6	0.262701	35	13.7091	13.3	10.4	3.5	105.1	34.8	0.222507
2	3.768	184	8.3	0.228305	13	5.2809	5.1	4	3.768	183.7	13	0.228954
3	4.253	334	15.2	0.474805	10	8.81328	8.5	6.7	4.253	334.5	10	0.498791
4	4.55	426	19.3	0.202308	12	3.84661	3.7	2.9	4.55	428.1	11.6	0.187417
5	4.95	538	24.4	0.170634	7	2.02271	2	1.5	4.95	537.4	6.9	0.165038
6	5.2	608	27.6	0.200212	8	2.82231	2.7	2.2	5.2	607.9	8.3	0.190859
7	5.251	632	28.7	0.122125	6	1.32513	1.3	1	5.251	631.6	6.5	0.115757
8	5.32	664	30.1	0.548262	6	5.27874	5.1	4	5.32	665.3	5.6	0.533869
9	5.649	818	37.1	0.432198	9	7.02014	6.8	5.3	5.649	818.3	8.8	0.448727
10	5.85	912	41.4	0.328245	7	3.8653	3.7	2.9	5.85	913.2	6.6	0.332378
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.98	971	44.1	0.577748	8	7.83014	7.6	6	5.98	972.6	7.8	0.568352
12	6.078	1016	46.1	0.379886	8	5.04723	4.9	3.8	6.078	1015.4	8.1	0.352013
13	6.16	1053	47.8	0.151771	14	3.65995	3.5	2.8	6.16	1048	14	0.14791
14	6.529	1221	55.4	0.176889	8	2.18294	2.1	1.7	6.529	1219	7.7	0.160431
15	6.685	1266	57.4	0.309907	12	4.87891	4.7	3.7	6.685	1268.5	11.6	0.236485
16	6.85	1309	59.4	0.0777439	5	0.704979	0.7	0.5	6.85	1308.8	5.1	0.0776946
17	7.35	1474	66.9	0.152336	9	2.49019	2.4	1.9	7.35	1474	9.2	0.152635
18	7.378	1484	67.3	0.241244	6	2.22467	2.2	1.7	7.378	1485.3	6	0.210078
19	7.886	1665	75.5	0.112207	9	1.68493	1.6	1.3	7.886	1665	8.5	0.111715
20	8.15	1759	79.8	0.111513	27	5.22205	5.1	4	8.15	1758.4	27	0.109017
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	1888	85.7	0.155868	12	3.20077	3.1	2.4	8.45	1887.7	11.9	0.15226
22	8.65	1958	88.8	0.152626	10	2.54214	2.5	1.9	8.65	1957.5	9.7	0.148253
23	9.3	2175	98.7	0.360857	13	7.50829	7.3	5.7	9.3	2171.2	12.6	0.336733

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.76	179	8.1	0.241398	12	5.25485	7.8	5.8	3.76	178.4	12.1	0.245895
2	4.254	328	14.9	0.536097	10	10.1897	15.2	11.3	4.254	327.6	10.2	0.563148
3	4.953	529	24	0.15477	6	1.70832	2.6	1.9	4.953	529.7	6.3	0.153265
4	5.249	621	28.2	0.130845	10	2.1263	3.2	2.4	5.249	617.2	10.4	0.115594
5	5.316	653	29.7	0.59856	6	5.80438	8.7	6.4	5.316	653.9	5.6	0.583799
6	5.646	810	36.8	0.499443	8	7.79239	11.6	8.6	5.646	810.9	8.5	0.517654
7	5.841	903	41	0.110386	7	1.26369	1.9	1.4	5.841	904.2	7.1	0.100409
8	5.971	963	43.7	0.630858	7	7.84657	11.7	8.7	5.971	964.5	7.2	0.61543
9	6.065	1006	45.7	0.426375	8	5.35525	8	5.9	6.065	1005.5	7.7	0.391835
10	6.143	1042	47.3	0.196328	13	4.35526	6.5	4.8	6.143	1038.5	13.2	0.185993
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.501	1206	54.8	0.197897	5	1.80875	2.7	2	6.501	1205.8	5.3	0.19124
12	6.547	1227	55.7	0.0657814	5	0.519503	0.8	0.6	6.547	1227.1	4.8	0.0608274
13	6.664	1259	57.2	0.171963	30	9.67345	14.4	10.7	6.664	1250.2	30	0.181926
14	7.27	1453	66	0.106205	6	1.07384	1.6	1.2	7.27	1452.9	5.7	0.106692
15	7.811	1646	74.8	0.163701	8	2.21162	3.3	2.4	7.811	1646.9	7.8	0.159407

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	100	4.5	0.292282	33	15.8828	22.2	18.8	3.5	101.6	32.9	0.272427
2	4.55	409	18.6	0.292348	9	4.42965	6.2	5.2	4.55	409.4	9.1	0.273381
3	5.2	587	26.7	0.422239	8	5.79117	8.1	6.8	5.2	587.1	7.8	0.418172
4	5.85	903	41	0.562237	7	7.02686	9.8	8.3	5.85	903.5	6.9	0.575523
5	6.55	1226	55.7	0.355163	8	5.02705	7	5.9	6.55	1225.8	8.5	0.33442
6	6.85	1309	59.5	0.261368	10	4.33265	6	5.1	6.85	1308.2	10.1	0.243053
7	7.35	1487	67.6	0.28769	11	5.1701	7.2	6.1	7.35	1484.8	10.5	0.277687
8	8.15	1776	80.7	0.106425	14	2.61818	3.7	3.1	8.15	1776.5	14.1	0.104444
9	8.45	1890	85.9	0.199924	11	3.7727	5.3	4.5	8.45	1889.1	11	0.193677
10	8.65	1955	88.8	0.171624	10	2.97215	4.1	3.5	8.65	1953.9	10.1	0.166638
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2175	98.8	0.365	34	14.6095	20.4	17.3	9.3	2160.8	33.6	0.245459

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 86

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 21.500 4.550 7.400 5.200 8.500 5.850 10.200 6.550 7.700 6.850 5.900 7.350 8.900 8.150 6.200 8.450 6.200 8.650 5.100 9.300 12.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.791 9.600 4.307 16.700 5.030 2.800 5.302 4.600 5.362 8.900 5.678 12.400 5.867 2.100 5.990 11.700 6.079 6.800 6.162 5.300 6.508 2.900 6.561 1.400 6.629 9.700 7.237 1.500 7.782 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 15 Return to Lane Navigator
3.500 22.900 4.550 6.700 5.200 8.500 5.850 10.700 6.550 7.900 6.850 6.400 7.350 9.100 8.150 5.800 8.450 5.800 8.650 4.400 9.300 11.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 13.300 3.768 5.100 4.253 8.500 4.550 3.700 4.950 2.000 5.200 2.700 5.251 1.300 5.320 5.100 5.649 6.800 5.850 3.700 5.980 7.600 6.078 4.900 6.160 3.500 6.529 2.100 6.685 4.700 6.850 0.700 7.350 2.400 7.378 2.200 7.886 1.600 8.150 5.100 8.450 3.100 8.650 2.500 9.300 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 23 Return to Lane Navigator
3.760 7.800 4.254 15.200 4.953 2.600 5.249 3.200 5.316 8.700 5.646 11.600 5.841 1.900 5.971 11.700 6.065 8.000 6.143 6.500 6.501 2.700 6.547 0.800 6.664 14.400 7.270 1.600 7.811 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 15 Return to Lane Navigator
3.500 22.200 4.550 6.200 5.200 8.100 5.850 9.800 6.550 7.000 6.850 6.000 7.350 7.200 8.150 3.700 8.450 5.300 8.650 4.100 9.300 20.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.