RFE Fish Species Identification Isoelectric Focusing Tools - Pataganian Toothfish Phast IEF Gel 02

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pataganian Toothfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Dissostichus eleginoides
FDA Seafood List Common Name: Pataganian Toothfish
FDA Seafood List Market Name: Patagonian Toothfish
Unique IEF Gel Code: 19980130gel04
Unique Web Code: pnh02Srr
Tissue Aliquot Code: disseleg L97-001 #05m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4.4	0.102135	6	0.961614	10.5	8.1	4.55	33.5	6	0.0907809
2	5.2	132	18	0.0988817	5	0.833527	9.1	7	5.2	131.6	4.8	0.0971976
3	5.85	234	31.9	0.0627223	6	0.636324	6.9	5.3	5.85	233.7	6	0.0598384
4	6.55	382	52.1	0.14017	7	1.6522	18	13.8	6.55	380.8	7	0.133321
5	6.85	414	56.5	0.0410762	7	0.454686	5	3.8	6.85	414.8	7.2	0.0357483
6	7.35	482	65.8	0.0862631	5	0.695055	7.6	5.8	7.35	481.9	4.8	0.0822014
7	8.15	604	82.4	0.0681771	13	1.50664	16.4	12.6	8.15	602.3	12.8	0.0663789
8	8.45	663	90.5	0.13114	7	1.63616	17.8	13.7	8.45	662	7.3	0.12575
9	8.65	716	97.7	0.097405	5	0.791037	8.6	6.6	8.65	715.6	4.8	0.0929725

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.771	57	7.8	0.0295841	4	0.147282	0.8	0.7	4.771	56	4	0.0207444
2	4.836	66	9	0.0309342	9	0.466196	2.5	2.1	4.836	66.3	9	0.0293357
3	5.071	99	13.5	0.243132	10	4.08185	22.1	18.7	5.071	99.5	9.8	0.234303
4	5.265	128	17.5	0.292301	3	1.66224	9	7.6	5.265	128.4	3.2	0.289083
5	5.4	151	20.6	0.195946	4	1.26719	6.9	5.8	5.4	151	3.6	0.198648
6	5.494	167	22.8	0.0262074	5	0.22895	1.2	1.1	5.494	167.4	5.3	0.0243848
7	5.624	189	25.8	0.210183	4	1.32138	7.2	6.1	5.624	189.6	3.8	0.194719
8	5.759	212	28.9	0.416563	4	3.32127	18	15.2	5.759	211.8	4.4	0.426214
9	5.866	231	31.5	0.110025	10	1.73862	9.4	8	5.866	230.3	10	0.0979206
10	5.944	248	33.8	0.0300786	4	0.168899	0.9	0.8	5.944	248.2	3.8	0.025326
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.193	302	41.2	0.109433	2	0.43207	2.3	2	6.193	301.8	2.2	0.110442
12	6.308	327	44.6	0.0495645	9	1.1062	6	5.1	6.308	333.2	8.9	0.0701254
13	6.359	338	46.1	0.241105	2	0.769348	4.2	3.5	6.359	337.9	2.3	0.186885
14	6.465	361	49.2	0.122428	7	1.52589	8.3	7	6.465	360.5	7.2	0.118865
15	6.77	403	55	0.0211432	3	0.097525	0.5	0.4	6.77	403.3	2.6	0.0211491
16	7.399	487	66.4	0.0150209	4	0.0942217	0.5	0.4	7.399	487.1	3.6	0.0148514

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	21	2.9	0.0414248	7	0.486521	6	4.4	4.55	22.3	6.8	0.0405083
2	5.2	103	14	0.0433615	8	0.545326	6.8	4.9	5.2	103.8	7.6	0.0407033
3	5.85	222	30.2	0.0799104	5	0.700291	8.7	6.3	5.85	222.1	5	0.0785922
4	6.55	378	51.4	0.133857	8	1.68078	20.9	15.2	6.55	376.5	7.6	0.125353
5	6.85	410	55.7	0.04666	9	0.649468	8.1	5.9	6.85	411.5	8.9	0.0410568
6	7.35	478	64.9	0.071518	6	0.656064	8.1	5.9	7.35	477.9	5.6	0.0658902
7	8.15	600	81.5	0.0546097	11	1.08981	13.5	9.8	8.15	598.3	11	0.0558025
8	8.45	658	89.4	0.119577	7	1.47009	18.2	13.3	8.45	656.7	7.1	0.117562
9	8.65	711	96.6	0.090169	5	0.781515	9.7	7.1	8.65	710.4	5.2	0.0850503

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	38	5.2	0.0621594	4	0.452347	2	1.7	4.55	38.2	4.4	0.0583313
2	4.766	62	8.4	0.0205337	1	0.00485111	0	0	4.766	62	0.8	0.00337266
3	4.838	70	9.5	0.0324735	12	0.606928	2.7	2.3	4.838	71.2	12.2	0.0281299
4	5.161	106	14.4	0.246121	10	4.26438	19.2	16.2	5.161	107.1	10.3	0.234513
5	5.306	132	17.9	0.316449	4	1.94147	8.7	7.4	5.306	131.3	3.8	0.290372
6	5.414	154	20.9	0.188605	3	1.1813	5.3	4.5	5.414	153.5	3.4	0.194502
7	5.497	171	23.2	0.0311804	4	0.194482	0.9	0.7	5.497	170.7	3.8	0.0286512
8	5.6	192	26.1	0.189496	4	1.09629	4.9	4.2	5.6	192.6	3.6	0.173976
9	5.713	215	29.2	0.390081	4	2.95119	13.3	11.2	5.713	214.5	4.3	0.387361
10	5.791	231	31.3	0.110106	8	1.33451	6	5.1	5.791	231.1	7.6	0.0988016
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	243	33	0.0198945	12	0.190825	0.9	0.7	5.85	232.7	11.8	0.0091555
12	5.881	249	33.8	0.00570075	1	0	0	0	5.881	246	1	0
13	6.156	302	41	0.0791749	2	0.33359	1.5	1.3	6.156	302.1	2.4	0.0787449
14	6.301	330	44.8	0.0777189	9	1.33425	6	5.1	6.301	332.8	8.9	0.0850404
15	6.358	341	46.3	0.236841	3	1.11447	5	4.2	6.358	340.8	3.4	0.184508
16	6.477	364	49.4	0.147206	10	2.40157	10.8	9.1	6.477	364.2	9.5	0.14209
17	6.55	378	51.3	0.0724629	7	0.697764	3.1	2.6	6.55	378.4	7.1	0.0553676
18	6.795	405	55	0.0159063	2	1.18E-6	0	0	6.795	404.9	2.4	2.81E-7
19	6.85	411	55.8	0.0222487	8	0.346981	1.6	1.3	6.85	410.6	7.9	0.0248881
20	7.35	478	64.9	0.0266823	11	0.439972	2	1.7	7.35	476.9	10.9	0.0228572
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.406	487	66.1	0.0206525	2	0.0354281	0.2	0.1	7.406	487.7	1.8	0.0112039
22	8.15	606	82.2	0.0256358	8	0.356669	1.6	1.4	8.15	604.2	7.9	0.0254785
23	8.45	665	90.2	0.0534052	6	0.593859	2.7	2.3	8.45	664.1	6.4	0.052336
24	8.65	714	96.9	0.0467628	5	0.380005	1.7	1.4	8.65	713.8	4.8	0.0450376

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.638	46	6.2	0.0263027	9	0.410511	2.2	1.9	4.638	49.6	8.8	0.0263298
2	4.701	54	7.3	0.0320576	4	0.109717	0.6	0.5	4.701	56.7	3.8	0.0162104
3	4.956	86	11.7	0.257538	9	3.663	20.1	17.1	4.956	86.9	8.6	0.240945
4	5.195	116	15.8	0.257672	3	1.51539	8.3	7.1	5.195	115.6	3.4	0.253997
5	5.32	139	18.9	0.159572	4	1.11235	6.1	5.2	5.32	138.9	3.9	0.160827
6	5.417	157	21.3	0.0197804	6	0.208949	1.1	1	5.417	155.3	6.3	0.0185716
7	5.53	178	24.2	0.154402	4	1.14904	6.3	5.4	5.53	178.9	4.3	0.150951
8	5.638	198	26.9	0.359108	4	2.64146	14.5	12.3	5.638	198.8	4.3	0.346067
9	5.734	216	29.3	0.10308	10	1.78193	9.8	8.3	5.734	215.6	10.4	0.096497
10	5.831	234	31.8	0.0189501	4	0.12148	0.7	0.6	5.831	233.5	4.4	0.0157418
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.105	288	39.1	0.0880864	3	0.452443	2.5	2.1	6.105	287.7	2.9	0.0882112
12	6.262	319	43.3	0.0755397	17	2.36049	12.9	11	6.262	322.2	17.2	0.0775915
13	6.318	330	44.8	0.205183	3	0.687542	3.8	3.2	6.318	330.4	2.8	0.140146
14	6.459	358	48.6	0.107006	9	1.75623	9.6	8.2	6.459	357.7	9.3	0.106798
15	6.79	402	54.6	0.0206055	7	0.210331	1.2	1	6.79	405	7.1	0.0167
16	7.395	484	65.8	0.0177673	3	0.0802739	0.4	0.4	7.395	483.6	2.5	0.017834

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4.3	0.0817527	6	0.785037	11.7	8.9	4.55	33.1	6.2	0.071597
2	5.2	123	16.7	0.0784719	5	0.635474	9.5	7.2	5.2	122.8	4.7	0.0770637
3	5.85	232	31.5	0.0392333	7	0.440425	6.6	5	5.85	231.4	6.9	0.0362459
4	6.55	374	50.8	0.0926334	8	1.23219	18.4	13.9	6.55	373.3	7.8	0.0894945
5	6.85	406	55.2	0.00815904	8	0.14708	2.2	1.7	6.85	405.4	8.3	0.00994777
6	7.35	476	64.7	0.0359913	5	0.300699	4.5	3.4	7.35	476.1	5	0.0336794
7	8.15	597	81.1	0.0706534	10	1.06502	15.9	12	8.15	597.2	9.5	0.0631697
8	8.45	660	89.7	0.114245	8	1.43164	21.4	16.1	8.45	659.2	7.5	0.107601
9	8.65	713	96.9	0.0833985	5	0.645137	9.7	7.3	8.65	712.5	4.6	0.0793897

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 83

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.500 5.200 9.100 5.850 6.900 6.550 18.000 6.850 5.000 7.350 7.600 8.150 16.400 8.450 17.800 8.650 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.771 0.800 4.836 2.500 5.071 22.100 5.265 9.000 5.400 6.900 5.494 1.200 5.624 7.200 5.759 18.000 5.866 9.400 5.944 0.900 6.193 2.300 6.308 6.000 6.359 4.200 6.465 8.300 6.770 0.500 7.399 0.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 16 Return to Lane Navigator
4.550 6.000 5.200 6.800 5.850 8.700 6.550 20.900 6.850 8.100 7.350 8.100 8.150 13.500 8.450 18.200 8.650 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 2.000 4.766 0 4.838 2.700 5.161 19.200 5.306 8.700 5.414 5.300 5.497 0.900 5.600 4.900 5.713 13.300 5.791 6.000 5.850 0.900 5.881 0 6.156 1.500 6.301 6.000 6.358 5.000 6.477 10.800 6.550 3.100 6.795 0 6.850 1.600 7.350 2.000 7.406 0.200 8.150 1.600 8.450 2.700 8.650 1.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 24 Return to Lane Navigator
4.638 2.200 4.701 0.600 4.956 20.100 5.195 8.300 5.320 6.100 5.417 1.100 5.530 6.300 5.638 14.500 5.734 9.800 5.831 0.700 6.105 2.500 6.262 12.900 6.318 3.800 6.459 9.600 6.790 1.200 7.395 0.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 11.700 5.200 9.500 5.850 6.600 6.550 18.400 6.850 2.200 7.350 4.500 8.150 15.900 8.450 21.400 8.650 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pataganian Toothfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pataganian Toothfish ---- Go to Display Gel Lane Navigator.