RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus macrocephalus
FDA Seafood List Common Name: Pacific Cod
FDA Seafood List Market Name: Cod (Alaska Cod)
Unique IEF Gel Code: 19970130gel03
Unique Web Code: pch03Srr
Tissue Aliquot Code: gadumacr E96-001 #08a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	59	7.4	0.178754	8	2.14505	14	10	4.55	60.9	7.9	0.152641
2	5.2	157	19.6	0.189846	4	1.40253	9.1	6.5	5.2	157	4.3	0.182589
3	5.85	248	30.9	0.226471	4	1.46861	9.6	6.9	5.85	248.3	3.7	0.226522
4	6.55	375	46.8	0.208799	6	2.1169	13.8	9.9	6.55	374.2	6.1	0.197019
5	6.85	408	50.9	0.116995	5	1.0361	6.7	4.8	6.85	408.7	5.3	0.109608
6	7.35	478	59.6	0.155757	5	1.31689	8.6	6.1	7.35	477.4	4.9	0.15298
7	8.15	604	75.3	0.148179	8	2.08896	13.6	9.8	8.15	603.8	8.2	0.143387
8	8.45	666	83	0.108308	9	1.76006	11.5	8.2	8.45	665.6	9.2	0.108102
9	8.65	722	90	0.0981508	5	0.824653	5.4	3.9	8.65	722.6	4.8	0.0964516
10	9.3	785	97.9	0.110921	7	1.19805	7.8	5.6	9.3	783	6.8	0.0992399

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.392	41	5.1	0.0177864	7	0.228297	1.3	1.2	4.392	42.4	7.2	0.0180083
2	4.461	51	6.3	0.0369899	4	0.213558	1.3	1.1	4.461	51.4	3.6	0.0331644
3	4.584	69	8.6	0.119142	7	1.0247	6	5.3	4.584	67.4	6.8	0.0847811
4	4.66	80	9.9	0.116069	10	1.86846	11	9.7	4.66	79.9	9.9	0.10647
5	4.756	94	11.7	0.0670987	9	0.848527	5	4.4	4.756	94.6	8.9	0.0535727
6	5.169	154	19.1	0.0365263	4	0.294008	1.7	1.5	5.169	155	4	0.0409783
7	5.252	166	20.6	0.375433	4	2.45625	14.4	12.8	5.252	166.4	3.7	0.373606
8	5.371	183	22.7	0.170294	5	1.47745	8.7	7.7	5.371	182.4	5	0.168174
9	5.665	225	28	0.21888	6	2.07217	12.2	10.8	5.665	224	5.9	0.197369
10	5.749	237	29.4	0.168182	10	2.50624	14.7	13	5.749	237.8	9.6	0.147815
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.88	257	31.9	0.0517661	13	1.42082	8.3	7.4	5.88	252.6	13	0.0614844
12	5.992	278	34.5	0.0422086	7	0.480524	2.8	2.5	5.992	278.7	7	0.0386819
13	6.078	294	36.5	0.0819633	4	0.614179	3.6	3.2	6.078	293.2	4.3	0.0803571
14	6.169	311	38.6	0.0791707	4	0.625141	3.7	3.3	6.169	311.8	4.4	0.0807956
15	6.255	327	40.6	0.0557527	5	0.520774	3.1	2.7	6.255	328	5.2	0.0567521
16	6.427	359	44.6	0.0405226	6	0.381129	2.2	2	6.427	358.8	6.1	0.0354477

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	65	8.1	0.125365	7	1.4791	12.5	8.5	4.55	66	7.3	0.11509
2	5.2	156	19.4	0.10641	7	1.41633	12	8.1	5.2	153.4	7.1	0.112641
3	5.85	251	31.2	0.162256	5	1.34567	11.4	7.7	5.85	250.4	4.7	0.160944
4	6.55	385	47.9	0.190441	6	1.75294	14.8	10.1	6.55	384.1	5.6	0.177638
5	6.85	417	51.9	0.10174	5	0.791871	6.7	4.5	6.85	416.9	4.6	0.0965514
6	7.35	483	60.1	0.119004	4	0.888184	7.5	5.1	7.35	482.4	4.3	0.115243
7	8.15	609	75.7	0.132103	7	1.49126	12.6	8.6	8.15	608.2	6.8	0.124635
8	8.45	668	83.1	0.10345	7	1.25524	10.6	7.2	8.45	667.2	7.1	0.0997375
9	8.65	724	90	0.0948699	4	0.663306	5.6	3.8	8.65	723.5	4	0.0925686
10	9.3	788	98	0.120998	3	0.754362	6.4	4.3	9.3	787.5	3.5	0.121964

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.114	11	1.4	0.0378123	5	0.364381	2	1.9	4.114	12	5.3	0.0386672
2	4.284	34	4.2	0.0255423	4	0.181647	1	0.9	4.284	34.3	4.1	0.0247691
3	4.432	54	6.7	0.0236074	4	0.154743	0.9	0.8	4.432	53.7	3.7	0.0238731
4	4.55	70	8.7	0.0633163	4	0.424751	2.4	2.2	4.55	69.1	4.4	0.0539995
5	4.661	85	10.6	0.035963	12	0.731621	4.1	3.8	4.661	83.9	11.9	0.0346099
6	4.749	97	12	0.0291974	5	0.192748	1.1	1	4.749	98.8	5.2	0.0208188
7	4.882	115	14.3	0.0090967	3	0.0463751	0.3	0.2	4.882	115.7	3.3	0.0079742
8	5.2	158	19.6	0.124266	7	1.47343	8.2	7.6	5.2	158.3	6.9	0.120513
9	5.266	168	20.9	0.3002	4	1.67482	9.3	8.6	5.266	168.3	3.5	0.268975
10	5.384	186	23.1	0.147925	5	1.16847	6.5	6	5.384	184.2	5.1	0.128922
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.679	231	28.7	0.155822	7	1.70142	9.4	8.7	5.679	230.3	7.1	0.135823
12	5.778	246	30.6	0.126824	11	2.33254	12.9	12	5.778	247.1	10.7	0.12346
13	5.85	257	31.9	0.10729	6	0.61773	3.4	3.2	5.85	258.7	6.2	0.0561016
14	6.022	288	35.8	0.0286987	7	0.320526	1.8	1.6	6.022	289.5	7.2	0.0249547
15	6.111	304	37.8	0.0585677	5	0.437097	2.4	2.2	6.111	303.5	4.5	0.0544189
16	6.211	322	40	0.0703453	4	0.455258	2.5	2.3	6.211	321.8	3.6	0.0721915
17	6.3	338	42	0.0604576	5	0.509556	2.8	2.6	6.3	337.9	4.8	0.05954
18	6.444	364	45.2	0.0385914	9	0.599834	3.3	3.1	6.444	366.7	9.2	0.0368368
19	6.55	383	47.6	0.091347	5	0.79798	4.4	4.1	6.55	382.3	5.2	0.0872799
20	6.85	416	51.7	0.0501559	5	0.382859	2.1	2	6.85	416.5	4.6	0.0472086
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	483	60	0.068805	5	0.546888	3	2.8	7.35	482.6	4.5	0.0683064
22	8.15	612	76	0.0794501	7	0.970397	5.4	5	8.15	612.5	7.1	0.0768027
23	8.45	678	84.2	0.0826735	6	0.92959	5.2	4.8	8.45	677.2	6.5	0.0807623
24	8.65	731	90.8	0.062255	5	0.538054	3	2.8	8.65	730.7	5	0.0607026
25	9.3	793	98.5	0.0788473	4	0.492572	2.7	2.5	9.3	792.7	3.6	0.0780657

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.129	15	1.9	0.0490784	6	0.483179	2.9	2.5	4.129	16	5.8	0.0468566
2	4.314	40	4.9	0.0640937	4	0.459053	2.7	2.4	4.314	40.7	3.9	0.0661383
3	4.424	55	6.8	0.0617181	6	0.586596	3.5	3.1	4.424	55.7	5.6	0.0590874
4	4.624	82	10.1	0.0528119	13	1.23518	7.3	6.5	4.624	78.5	13.5	0.0516901
5	4.735	97	12	0.0160193	3	0.0342388	0.2	0.2	4.735	96.8	2.8	0.0068453
6	5.163	155	19.2	0.0464159	4	0.28275	1.7	1.5	5.163	154	3.6	0.0447606
7	5.245	167	20.6	0.435056	3	2.69072	15.9	14.2	5.245	167.4	3.5	0.434253
8	5.373	187	23.1	0.270771	3	1.609	9.5	8.5	5.373	186.7	3.5	0.260174
9	5.693	237	29.3	0.297414	4	1.90909	11.3	10.1	5.693	236.3	4.1	0.262883
10	5.764	248	30.7	0.230845	10	3.89017	23	20.5	5.764	249.9	9.9	0.221767
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.887	268	33.1	0.0687824	8	0.81727	4.8	4.3	5.887	266.1	7.6	0.0603159
12	5.954	280	34.6	0.0551728	4	0.359754	2.1	1.9	5.954	281	3.7	0.0549548
13	6.044	296	36.6	0.108595	3	0.520337	3.1	2.7	6.044	295.5	2.6	0.112185
14	6.151	315	38.9	0.113276	4	0.762276	4.5	4	6.151	313.7	3.8	0.114381
15	6.241	331	40.9	0.096315	6	0.9123	5.4	4.8	6.241	331.3	5.6	0.0918528
16	6.398	359	44.4	0.0282177	8	0.359193	2.1	1.9	6.398	362.8	8.1	0.0251069

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	72	8.9	0.197573	8	2.32196	14.7	11.1	4.55	73.1	7.9	0.166707
2	5.2	160	19.8	0.164915	6	1.76277	11.2	8.4	5.2	159.6	6.1	0.162145
3	5.85	264	32.6	0.167175	6	1.6298	10.3	7.8	5.85	264.2	5.7	0.160413
4	6.55	387	47.8	0.198715	6	2.09512	13.3	10	6.55	386.1	6.3	0.188351
5	6.85	423	52.3	0.0617205	7	0.725523	4.6	3.5	6.85	423.9	6.8	0.0605015
6	7.35	489	60.4	0.116874	7	1.3578	8.6	6.5	7.35	489.2	6.9	0.111505
7	8.15	614	75.9	0.132467	10	2.2796	14.5	10.9	8.15	613.8	10.3	0.124608
8	8.45	677	83.7	0.111107	11	2.16305	13.7	10.4	8.45	679	10.8	0.113271
9	8.65	726	89.7	0.0862731	7	1.02629	6.5	4.9	8.65	727.9	7	0.0821419
10	9.3	796	98.4	0.0596194	4	0.403799	2.6	1.9	9.3	795.4	3.9	0.0590648

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 87

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 14.000 5.200 9.100 5.850 9.600 6.550 13.800 6.850 6.700 7.350 8.600 8.150 13.600 8.450 11.500 8.650 5.400 9.300 7.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.392 1.300 4.461 1.300 4.584 6.000 4.660 11.000 4.756 5.000 5.169 1.700 5.252 14.400 5.371 8.700 5.665 12.200 5.749 14.700 5.880 8.300 5.992 2.800 6.078 3.600 6.169 3.700 6.255 3.100 6.427 2.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 16 Return to Lane Navigator
4.550 12.500 5.200 12.000 5.850 11.400 6.550 14.800 6.850 6.700 7.350 7.500 8.150 12.600 8.450 10.600 8.650 5.600 9.300 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.114 2.000 4.284 1.000 4.432 0.900 4.550 2.400 4.661 4.100 4.749 1.100 4.882 0.300 5.200 8.200 5.266 9.300 5.384 6.500 5.679 9.400 5.778 12.900 5.850 3.400 6.022 1.800 6.111 2.400 6.211 2.500 6.300 2.800 6.444 3.300 6.550 4.400 6.850 2.100 7.350 3.000 8.150 5.400 8.450 5.200 8.650 3.000 9.300 2.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 25 Return to Lane Navigator
4.129 2.900 4.314 2.700 4.424 3.500 4.624 7.300 4.735 0.200 5.163 1.700 5.245 15.900 5.373 9.500 5.693 11.300 5.764 23.000 5.887 4.800 5.954 2.100 6.044 3.100 6.151 4.500 6.241 5.400 6.398 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 14.700 5.200 11.200 5.850 10.300 6.550 13.300 6.850 4.600 7.350 8.600 8.150 14.500 8.450 13.700 8.650 6.500 9.300 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.