RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Golden Redfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes norvegicus
FDA Seafood List Common Name: Golden Redfish
FDA Seafood List Market Name: Perch, Ocean
Unique IEF Gel Code: 19940908gel02
Unique Web Code: oph01Srr
Tissue Aliquot Code: sebanorv H94-003 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4.1	0.0692285	4	0.466017	7.1	5.6	4.55	32.2	4	0.0656613
2	5.2	130	16.8	0.11578	4	0.733847	11.3	8.9	5.2	129.8	3.7	0.112125
3	5.85	238	30.8	0.150493	4	1.09595	16.8	13.3	5.85	237.7	4.3	0.144509
4	6.55	374	48.4	0.0599615	7	0.724704	11.1	8.8	6.55	372	6.8	0.0597555
5	7.35	467	60.4	0.0349864	5	0.332664	5.1	4	7.35	465.9	5.5	0.0343184
6	8.15	584	75.5	0.0466148	9	0.684325	10.5	8.3	8.15	585.3	8.6	0.0446365
7	8.45	649	84	0.0797045	6	0.861665	13.2	10.4	8.45	648.1	6.2	0.0781498
8	8.65	698	90.3	0.0500839	4	0.37541	5.8	4.5	8.65	697.8	4.5	0.0475423
9	9.3	746	96.5	0.126024	6	1.24656	19.1	15.1	9.3	745.7	6	0.117058

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.659	50	6.4	0.269871	14	6.32952	32.4	19.3	4.659	53.5	13.5	0.264082
2	4.947	92	11.8	0.152521	17	4.26241	21.8	13	4.947	90.4	16.7	0.143921
3	5.82	232	29.8	0.0627695	5	0.51411	2.6	1.6	5.82	232.4	4.8	0.0602835
4	5.934	253	32.5	0.454936	3	2.68378	13.7	8.2	5.934	252.8	3.3	0.460002
5	5.965	259	33.3	0.156007	3	0.815925	4.2	2.5	5.965	259.8	2.9	0.159285
6	6.017	269	34.6	0.165183	5	1.43527	7.3	4.4	6.017	269.1	5.4	0.149816
7	6.158	296	38	0.110842	3	0.606387	3.1	1.8	6.158	295.9	3.1	0.11101
8	6.19	302	38.8	0.262426	2	1.00166	5.1	3.1	6.19	302.4	2.2	0.261106
9	6.325	328	42.2	0.0540495	5	0.50171	2.6	1.5	6.325	327.2	5.1	0.0553021
10	6.383	339	43.6	0.0126632	1	0.0229497	0.1	0.1	6.383	339.1	1.4	0.0094296
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.477	357	45.9	0.0526937	5	0.466796	2.4	1.4	6.477	357.3	5.2	0.0506305
12	6.738	394	50.6	0.0862065	3	0.526734	2.7	1.6	6.738	393.9	3.4	0.086866
13	7.364	471	60.5	0.03323	7	0.370497	1.9	1.1	7.364	473.1	7.1	0.0292734

PeakCounter: 13

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	28	3.6	0.062232	6	0.595366	9.3	7	4.55	28.5	6.2	0.0542158
2	5.2	120	15.5	0.105543	4	0.68932	10.7	8.1	5.2	119.4	3.7	0.104383
3	5.85	228	29.5	0.134113	4	0.970932	15.1	11.5	5.85	228	4.3	0.127418
4	6.55	360	46.6	0.074177	7	0.889673	13.9	10.5	6.55	360.5	7.3	0.069234
5	7.35	463	59.9	0.0308044	6	0.305258	4.8	3.6	7.35	463.4	5.7	0.0303085
6	8.15	580	75	0.0443452	9	0.635611	9.9	7.5	8.15	580.8	8.6	0.0415404
7	8.45	645	83.4	0.0751662	6	0.815311	12.7	9.6	8.45	645.1	6.3	0.0726902
8	8.65	692	89.5	0.0476694	4	0.343159	5.3	4.1	8.65	692.3	4.2	0.0463174
9	9.3	744	96.2	0.105947	7	1.16962	18.2	13.8	9.3	743.5	6.7	0.0988449

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	34	4.4	0.0662287	8	0.42188	2.2	1.4	4.55	42.4	8.1	0.0293645
2	4.673	51	6.6	0.225727	17	6.32009	32.9	20.6	4.673	53.1	16.6	0.215063
3	4.947	89	11.5	0.166705	12	3.58449	18.7	11.7	4.947	88.8	11.8	0.170675
4	5.2	124	16	0.0434721	4	0.267206	1.4	0.9	5.2	124.2	3.5	0.0426245
5	5.789	230	29.7	0.0208571	3	0.107869	0.6	0.4	5.789	230.1	2.8	0.0216453
6	5.85	241	31.1	0.0103958	2	0.0388737	0.2	0.1	5.85	238.8	1.8	0.0119757
7	5.902	250	32.3	0.334527	3	2.08316	10.8	6.8	5.902	250.5	3.5	0.336872
8	5.965	261	33.7	0.10463	4	0.557035	2.9	1.8	5.965	259.5	4.4	0.0709832
9	5.994	266	34.3	0.106662	5	0.893058	4.6	2.9	5.994	266.5	5.3	0.0949725
10	6.166	296	38.2	0.0670917	6	0.712804	3.7	2.3	6.166	296.4	6	0.0670115
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.212	304	39.2	0.140898	2	0.536417	2.8	1.7	6.212	303.6	2.4	0.127102
12	6.368	331	42.7	0.0328583	7	0.398079	2.1	1.3	6.368	330.7	7.5	0.0299512
13	6.42	340	43.9	0.0220207	2	0.0469376	0.2	0.2	6.42	339.7	2.1	0.0124631
14	6.517	357	46.1	0.0603703	7	0.78122	4.1	2.5	6.517	358.3	7.5	0.0588018
15	6.79	393	50.7	0.0632106	4	0.42212	2.2	1.4	6.79	392.4	4.2	0.0570097
16	7.386	469	60.5	0.0467704	8	0.651904	3.4	2.1	7.386	466.9	8.2	0.0450519
17	8.45	657	84.8	0.0447138	7	0.513273	2.7	1.7	8.45	656.2	6.6	0.0439873
18	8.65	701	90.5	0.024214	4	0.172005	0.9	0.6	8.65	700.1	3.9	0.0245882
19	9.3	748	96.5	0.057043	8	0.701567	3.7	2.3	9.3	746.9	7.5	0.0526467

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.67	46	6	0.222272	15	6.19869	33.8	17.1	4.67	45.6	15.3	0.229004
2	4.946	86	11.1	0.149787	14	3.68864	20.1	10.2	4.946	82.8	13.8	0.151267
3	5.787	226	29.2	0.0531621	4	0.347554	1.9	1	5.787	225.8	3.7	0.0534783
4	5.884	243	31.4	0.495216	3	2.69001	14.7	7.4	5.884	242.8	3.1	0.494089
5	5.934	252	32.6	0.154455	4	0.756185	4.1	2.1	5.934	249.8	4.1	0.104776
6	5.963	257	33.2	0.164744	5	1.36659	7.5	3.8	5.963	256.9	5.3	0.146618
7	6.126	286	37	0.0837597	4	0.538897	2.9	1.5	6.126	285.5	3.7	0.082011
8	6.165	293	37.9	0.210788	2	0.813487	4.4	2.2	6.165	293.3	2.1	0.216086
9	6.334	323	41.8	0.0572354	10	0.86547	4.7	2.4	6.334	321.9	9.9	0.0494427
10	6.385	332	42.9	0.0251783	1	0	0	0	6.385	329	1	0
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.486	350	45.3	0.0521829	5	0.46567	2.5	1.3	6.486	350.9	5.2	0.0505872
12	6.749	386	49.9	0.0801569	3	0.427333	2.3	1.2	6.749	385.8	3.1	0.076949
13	7.387	466	60.3	0.0236231	4	0.157973	0.9	0.4	7.387	465.8	4	0.0220779

PeakCounter: 13

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.1	0.0824156	4	0.607589	7.8	6.3	4.55	24.4	4.4	0.0787028
2	5.2	123	16	0.110794	4	0.753147	9.7	7.8	5.2	122.1	3.8	0.110574
3	5.85	234	30.4	0.154703	4	1.01823	13.1	10.6	5.85	234.1	3.8	0.15252
4	6.55	361	46.8	0.0648345	7	0.813135	10.5	8.4	6.55	361.7	6.9	0.0662045
5	7.35	458	59.4	0.0463767	6	0.415978	5.4	4.3	7.35	458.2	5.6	0.0422636
6	8.15	584	75.7	0.091134	7	1.10403	14.3	11.5	8.15	583.7	7.1	0.0877853
7	8.45	652	84.6	0.0932178	7	1.16915	15.1	12.1	8.45	650.8	7.1	0.0924682
8	8.65	699	90.7	0.0664369	4	0.488923	6.3	5.1	8.65	698.3	4.3	0.063517
9	9.3	746	96.8	0.135454	6	1.37352	17.7	14.3	9.3	745.2	5.9	0.130434

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 72

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.100 5.200 11.300 5.850 16.800 6.550 11.100 7.350 5.100 8.150 10.500 8.450 13.200 8.650 5.800 9.300 19.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.659 32.400 4.947 21.800 5.820 2.600 5.934 13.700 5.965 4.200 6.017 7.300 6.158 3.100 6.190 5.100 6.325 2.600 6.383 0.100 6.477 2.400 6.738 2.700 7.364 1.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 13 Return to Lane Navigator
4.550 9.300 5.200 10.700 5.850 15.100 6.550 13.900 7.350 4.800 8.150 9.900 8.450 12.700 8.650 5.300 9.300 18.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 2.200 4.673 32.900 4.947 18.700 5.200 1.400 5.789 0.600 5.850 0.200 5.902 10.800 5.965 2.900 5.994 4.600 6.166 3.700 6.212 2.800 6.368 2.100 6.420 0.200 6.517 4.100 6.790 2.200 7.386 3.400 8.450 2.700 8.650 0.900 9.300 3.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
4.670 33.800 4.946 20.100 5.787 1.900 5.884 14.700 5.934 4.100 5.963 7.500 6.126 2.900 6.165 4.400 6.334 4.700 6.385 0 6.486 2.500 6.749 2.300 7.387 0.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 13 Return to Lane Navigator
4.550 7.800 5.200 9.700 5.850 13.100 6.550 10.500 7.350 5.400 8.150 14.300 8.450 15.100 8.650 6.300 9.300 17.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Golden Redfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Golden Redfish ---- Go to Display Gel Lane Navigator.