Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus synagris
FDA Seafood List Common Name: Lane Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940823gel03
Unique Web Code: lsh03Srr
Tissue Aliquot Code: lutjsyna H94-002 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5510712.50.056771770.60036810.98.84.55107.26.50.0518618
25.220924.30.05002260.5382999.87.95.2208.76.50.0470621
35.8532237.50.057432670.660375129.75.85322.970.0532984
46.5545252.60.046205680.64654211.89.56.55450.48.10.0448981
57.3554963.90.026777470.3244955.94.87.35550.77.40.0248032
68.1567678.70.039000880.58452810.68.68.15674.48.50.0388537
78.4573685.70.081259970.97964217.814.48.45735.76.90.0801788
88.65782910.052859260.5279439.67.88.65781.85.80.0516712
99.382796.30.054862470.62653411.49.29.3826.270.0505276
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.05323.70.17309471.994628.574.05336.70.167943
24.75614016.30.21072462.220749.57.74.756140.46.40.195585
34.91916519.20.118699112.1184497.44.919161.610.90.109835
45.29422426.10.024517240.1613270.70.65.294223.53.60.0256196
55.3723727.60.46965832.3987610.28.45.37236.93.10.441827
65.46425329.50.0690428131.418976.14.95.464248.313.10.0613312
75.58127331.80.066477860.6131022.62.15.5812735.70.0610002
85.90332938.30.021545970.23720310.85.903331.26.60.0202537
96.00834940.70.50398932.7052311.59.46.008349.33.10.495205
106.08236342.30.21179841.352065.84.76.082363.33.80.202292
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.16737944.20.12540851.218875.24.26.167377.65.20.131834
126.2339145.60.009503730.02871380.10.16.23393.53.20.0050005
136.28840246.90.012344320.03308850.10.16.288401.81.70.0112766
146.33141047.80.029266130.1328310.60.56.331410.32.60.0290662
156.44743250.30.053009660.5254132.21.86.447431.76.40.0461895
166.544251.50.018615230.08049740.30.36.5441.72.70.0167433
176.68346854.50.41892242.9637112.610.36.683467.640.420543
186.84548856.90.06411880.8456633.62.96.845486.58.20.0578457
196.95850258.50.19120941.199465.14.26.958502.63.70.181854
207.08751860.40.01542630.07040910.30.27.087518.22.60.0154369
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.24153762.60.0272930.141120.60.57.241536.530.026615
227.37955564.70.06783780.9690334.13.47.379555.78.20.0665521
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55111130.054863970.58139810.88.14.55111.46.60.049759
25.220824.30.070568640.5271629.87.35.2207.74.50.0666459
35.85317370.080353650.67872912.69.45.85317.15.40.0708766
46.5545252.70.067176460.69695812.99.76.55451.66.10.0645366
57.3555364.50.024719760.2630494.93.77.35553.16.40.0232141
68.1567478.60.03743580.5196979.67.28.15673.580.0366521
78.4573585.80.092590560.95883617.813.38.45734.65.90.0911179
88.6577790.70.077801850.61433311.48.58.65776.84.60.0753171
99.382596.30.056094860.55082210.27.79.3825.15.90.0527463
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.985414.80.16013861.599147.66.33.98541.45.70.157228
24.5512114.10.024764560.2305181.10.94.55120.75.70.0228171
34.76215117.60.1826772.04399.78.14.762152.96.70.171482
44.92417420.30.13891692.075059.88.24.924170.98.70.134488
55.221324.90.016079450.1253960.60.55.2213.34.60.0153207
65.29422926.80.02168450.1849740.90.75.294227.84.60.0226418
75.39324628.70.32461942.1518610.28.55.393245.53.90.308075
85.505265310.0403865150.9129714.33.65.505259.314.90.0345345
95.63328733.50.046551850.345311.61.45.633287.14.50.0431169
105.8532437.90.034718230.205510.85.85324.13.20.0357098
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.04635941.90.010490330.05276240.20.26.046357.92.80.0105092
126.10837043.20.36301731.789198.57.16.108370.32.90.351302
136.17538244.60.19486541.203335.74.86.175382.33.70.18552
146.26439846.50.10421361.0525954.26.264396.75.90.101101
156.40442349.40.019899520.05520920.30.26.404423.21.70.0186053
166.50544151.50.064184760.6192492.92.46.505441.16.30.0552434
176.5544952.50.041124350.2453261.216.55450.45.30.026143
186.72147255.10.48789532.6865312.710.66.721472.13.10.484371
196.86249157.40.056866790.7713983.736.862489.58.80.0495659
206.966505590.16212241.0479454.16.966505.83.80.157421
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.07852060.70.013133530.05823510.30.27.078520.12.60.012769
227.23454163.20.014363920.0513170.20.27.234540.32.40.0119774
237.37956165.50.0327866130.7107053.42.87.379561.612.90.0311313
248.15676790.014887260.1411490.70.68.15676.45.60.0141564
258.4574486.90.019460290.2858241.41.18.457428.70.0184921
268.6578291.40.015239170.1829870.90.78.65781.570.0146701
279.382996.80.019617490.285881.41.19.38289.50.0169965
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0054350.31336963.1259411.99.54.00543.25.70.307797
24.77515618.20.34617652.7425810.48.34.775156.24.90.315268
34.938180210.142505133.0467611.69.34.938174.613.10.130884
45.29723527.50.053743650.4230641.61.35.297234.24.70.0511526
55.38525029.20.50390332.455119.37.55.385249.93.10.445101
65.48526731.20.0598643131.785036.85.45.485257.213.40.0753218
75.626291340.065489840.4398711.71.35.626291.140.0615516
86.0436342.40.017915820.05302270.20.26.04363.11.60.0185446
96.0837043.20.42861421.520945.84.66.08369.71.90.440112
106.1337944.30.28388841.646396.356.133793.50.264713
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.214394460.11942961.094574.23.36.214393.25.60.110195
126.3642049.10.043389560.368951.41.16.36418.75.70.0366736
136.47744151.50.087321650.6398142.41.96.477440.74.60.078936
146.57345753.40.043935440.3188471.216.573457.340.0454635
156.64846754.60.048499640.3443131.316.6484674.20.0465369
166.747455.40.51204432.8388710.88.66.7474.23.20.50348
176.84349357.60.068703660.7237992.82.26.843491.86.20.0657779
186.96450959.50.23441931.330445.146.9645093.20.231759
197.09252661.40.016074850.146040.60.47.092526.15.10.0159997
207.257548640.027428640.1867620.70.67.257547.73.80.0278107
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.41557066.60.070885191.082684.13.37.415570.28.70.0705818
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5512114.20.06810360.66982412.89.84.55121.86.30.0598145
25.222025.80.058630650.52788610.17.75.2220.45.40.0553649
35.8533038.70.079789150.628729129.25.85329.54.90.0727347
46.5545553.40.056838470.69289513.210.16.55455.67.30.0538833
57.3556065.70.024125150.2052063.937.35559.74.90.0237744
68.1567779.50.0332315110.62159311.99.18.15674.210.60.0332069
78.4573486.20.0723170.81392115.611.98.45734.36.60.0700933
88.6578291.80.058212140.4498.66.58.65782.14.40.057358
99.382897.20.054543970.62417111.99.19.38287.20.0490691
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic

 
Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 97
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse   |   RFE Tutorial   |   Seafoods   |   CFSAN


Last updated on 2003-FEB-27 by bjt/frf/nln