RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes pinniger
FDA Seafood List Common Name: Canary Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19930818gel01
Unique Web Code: cag02Srr
Tissue Aliquot Code: sebapinn 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	17	1.5	0.547109	4	4.07643	19.6	11.4	3.5	18	4.3	0.532663
2	4.55	168	14.7	0.296971	4	2.02441	9.7	5.7	4.55	168.2	4.3	0.264038
3	5.2	258	22.6	0.360978	4	2.28224	11	6.4	5.2	257.9	3.6	0.353609
4	5.85	422	36.9	0.229371	9	3.23652	15.6	9.1	5.85	418.6	8.5	0.214528
5	6.55	639	55.9	0.29927	5	2.47358	11.9	6.9	6.55	638.1	4.9	0.282301
6	6.85	677	59.2	0.145969	6	1.44035	6.9	4	6.85	676.9	6	0.135127
7	7.35	765	66.9	0.315801	5	3.01687	14.5	8.4	7.35	763.7	5.5	0.309826
8	8.15	922	80.7	0.0441926	15	1.10895	5.3	3.1	8.15	919.5	15.2	0.041117
9	8.45	975	85.3	0.0639341	7	0.812409	3.9	2.3	8.45	975.4	7.3	0.0628625
10	8.65	1007	88.1	0.0257823	7	0.311681	1.5	0.9	8.65	1006.9	6.9	0.0254857

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	22	1.9	0.499016	4	3.19673	8.7	6.7	3.5	22.9	3.6	0.497523
2	4.159	118	10.3	0.213543	4	1.42551	3.9	3	4.159	118.5	3.8	0.209071
3	4.44	159	13.9	0.0602262	4	0.430789	1.2	0.9	4.44	159.5	4.3	0.0565856
4	4.55	175	15.3	0.193981	5	1.51582	4.1	3.2	4.55	175.5	4.5	0.188488
5	4.65	189	16.5	0.516249	4	3.60114	9.9	7.6	4.65	189.5	3.9	0.524992
6	4.921	227	19.8	0.0107233	1	0	0	0	4.921	223	1	0
7	5.014	240	21	0.0901449	10	1.9202	5.3	4	5.014	245.9	10.1	0.1076
8	5.2	266	23.2	0.22901	7	2.54035	7	5.3	5.2	266.4	6.8	0.209392
9	5.85	423	36.9	0.115923	11	2.41893	6.6	5.1	5.85	418.7	11.3	0.121068
10	5.919	444	38.8	0.0746265	4	0.527573	1.4	1.1	5.919	445.7	4.3	0.0695078
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.992	466	40.7	0.0795534	8	1.09441	3	2.3	5.992	466.1	7.7	0.0800007
12	6.088	495	43.2	0.0529017	6	0.564684	1.5	1.2	6.088	496.8	5.6	0.0572171
13	6.147	513	44.8	0.230916	8	3.19008	8.7	6.7	6.147	513.2	8.1	0.222228
14	6.329	568	49.6	0.0457581	10	0.761465	2.1	1.6	6.329	568.4	10.3	0.0416485
15	6.405	591	51.6	0.119285	8	1.55107	4.2	3.3	6.405	590.3	7.7	0.113337
16	6.55	635	55.5	0.143399	11	2.4955	6.8	5.2	6.55	635.7	10.6	0.132419
17	6.85	674	58.9	0.0702732	7	0.919203	2.5	1.9	6.85	673.9	7.4	0.0698075
18	7.35	764	66.7	0.246976	6	2.87102	7.9	6	7.35	763.3	6.4	0.253666
19	7.538	800	69.9	0.0444807	14	1.10587	3	2.3	7.538	798.6	14.4	0.0434289
20	7.643	820	71.6	0.196545	5	1.57066	4.3	3.3	7.643	819.3	4.7	0.190055
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.15	917	80.1	0.0209751	14	0.499939	1.4	1.1	8.15	917.6	14.1	0.0199997
22	8.45	981	85.7	0.0959825	12	1.90639	5.2	4	8.45	981.3	11.9	0.0904365
23	8.65	1010	88.2	0.0361162	7	0.435072	1.2	0.9	8.65	1010.5	7.2	0.0338929

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.131	105	9.3	0.18986	4	1.20693	4.7	3.5	4.131	105.5	3.6	0.18954
2	4.42	147	13	0.029978	3	0.16914	0.7	0.5	4.42	147	3.4	0.0283324
3	4.629	177	15.6	0.546767	4	3.82675	14.8	11.2	4.629	177.4	3.9	0.55954
4	4.911	216	19	0.00968084	1	0	0	0	4.911	212	1	0
5	5.005	229	20.2	0.103263	11	2.30072	8.9	6.7	5.005	233.7	10.7	0.120923
6	5.142	248	21.9	0.0521977	7	0.528837	2	1.5	5.142	251.2	7.2	0.0414241
7	5.862	416	36.7	0.47825	3	2.57211	10	7.5	5.862	415.9	3.1	0.47214
8	5.923	434	38.2	0.017877	2	0.0764068	0.3	0.2	5.923	433	2.5	0.0175092
9	5.971	448	39.5	0.362745	4	1.94413	7.5	5.7	5.971	447.7	3.6	0.304687
10	6.012	460	40.5	0.0682887	11	1.41748	5.5	4.1	6.012	455.4	10.7	0.0744502
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.07	477	42	0.0908174	2	0.359564	1.4	1.1	6.07	477.2	2.5	0.0813259
12	6.098	485	42.7	0.0826378	2	0.260243	1	0.8	6.098	484.3	2.1	0.0714905
13	6.135	496	43.7	0.456849	3	2.25709	8.7	6.6	6.135	495.6	2.9	0.441489
14	6.313	548	48.3	0.0237543	5	0.196458	0.8	0.6	6.313	548.3	5.4	0.0204143
15	6.378	567	50	0.134985	4	0.99835	3.9	2.9	6.378	567.7	4.4	0.128702
16	6.466	593	52.2	0.0583361	3	0.27994	1.1	0.8	6.466	592.7	2.9	0.0542392
17	6.504	604	53.2	0.125123	7	1.43162	5.5	4.2	6.504	606.1	7.5	0.107751
18	7.339	743	65.5	0.0229212	3	0.135557	0.5	0.4	7.339	743	3.2	0.0241587
19	7.536	782	68.9	0.0766406	18	2.13486	8.3	6.2	7.536	782.7	18	0.0669161
20	7.617	798	70.3	0.276052	3	1.43622	5.6	4.2	7.617	797.9	3.4	0.235727
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.438	964	84.9	0.0920461	15	2.30215	8.9	6.7	8.438	964.4	14.7	0.0881532

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	21	1.8	0.605379	4	4.39505	20.2	12	3.5	21.3	4.2	0.590062
2	4.55	174	15.2	0.301257	4	1.97002	9	5.4	4.55	174.1	4.1	0.272176
3	5.2	263	23	0.409481	4	2.71842	12.5	7.4	5.2	263.1	3.9	0.393101
4	5.85	419	36.7	0.354148	6	3.06133	14	8.3	5.85	417.5	5.8	0.29956
5	6.55	617	54.1	0.287624	5	2.23636	10.3	6.1	6.55	617.2	4.7	0.269373
6	6.85	656	57.5	0.182969	6	1.73646	8	4.7	6.85	656.1	5.6	0.173474
7	7.35	743	65.1	0.392851	4	3.04493	14	8.3	7.35	742.4	4.5	0.38294
8	8.15	908	79.6	0.048384	16	1.30564	6	3.6	8.15	907.7	15.7	0.046799
9	8.45	969	84.9	0.0688337	8	0.951507	4.4	2.6	8.45	968.5	8.2	0.0653782
10	8.65	1003	87.9	0.0249085	9	0.384167	1.8	1	8.65	999.3	8.7	0.024794

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	20	1.8	0.498853	5	4.63882	10.3	8.2	3.5	21.4	5.3	0.489829
2	4.153	112	9.8	0.253952	4	1.61729	3.6	2.9	4.153	112.3	3.7	0.244127
3	4.436	152	13.3	0.0665132	4	0.410667	0.9	0.7	4.436	152.4	3.6	0.0636872
4	4.55	168	14.7	0.235649	4	1.54049	3.4	2.7	4.55	168.1	3.9	0.223459
5	4.652	182	16	0.578389	4	3.86187	8.6	6.8	4.652	181.8	3.7	0.581741
6	4.93	220	19.3	0.0344566	3	0.160269	0.4	0.3	4.93	219.1	3	0.0301865
7	5.017	232	20.4	0.0913723	11	3.28097	7.3	5.8	5.017	240.3	11.5	0.16101
8	5.2	257	22.5	0.268989	5	2.03274	4.5	3.6	5.2	256.9	4.7	0.243187
9	5.85	414	36.3	0.45639	4	3.46953	7.7	6.1	5.85	413.4	4.1	0.475775
10	5.909	431	37.8	0.153871	7	1.77264	4	3.1	5.909	430.9	6.7	0.150062
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.958	445	39	0.274764	6	2.67085	6	4.7	5.958	444.6	6	0.252943
12	6.031	466	40.9	0.0385873	3	0.222685	0.5	0.4	6.031	467.1	3.2	0.0394937
13	6.069	477	41.8	0.0678112	4	0.43999	1	0.8	6.069	476.7	4	0.0619601
14	6.097	485	42.5	0.0762206	3	0.363468	0.8	0.6	6.097	484.6	2.7	0.0759487
15	6.139	497	43.6	0.46959	4	3.04821	6.8	5.4	6.139	496.1	3.7	0.46618
16	6.324	550	48.2	0.0191486	6	0.189037	0.4	0.3	6.324	547.8	5.7	0.0186658
17	6.39	569	49.9	0.0805327	6	0.775641	1.7	1.4	6.39	570.5	6.2	0.0705147
18	6.505	602	52.8	0.0933554	5	0.816857	1.8	1.4	6.505	602	5.4	0.0853329
19	6.55	615	53.9	0.235363	5	2.14452	4.8	3.8	6.55	614.1	5	0.244112
20	6.85	655	57.5	0.121076	6	1.31553	2.9	2.3	6.85	654.1	6.4	0.115249
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	749	65.7	0.359532	5	3.35554	7.5	5.9	7.35	748	5.4	0.353447
22	7.561	791	69.4	0.0629935	12	1.192	2.7	2.1	7.561	792.4	11.5	0.0584466
23	7.637	806	70.7	0.272659	4	1.81983	4.1	3.2	7.637	806.3	4	0.256997
24	8.15	908	79.6	0.033749	19	1.04077	2.3	1.8	8.15	906	18.6	0.0314867
25	8.45	971	85.2	0.101374	13	2.15102	4.8	3.8	8.45	971	12.6	0.0963169
26	8.65	998	87.5	0.0340875	10	0.517726	1.2	0.9	8.65	997.4	9.8	0.0298316

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.136	112	9.8	0.196942	4	1.21293	4.4	3.4	4.136	111.6	3.5	0.193613
2	4.421	152	13.3	0.0308009	3	0.156968	0.6	0.4	4.421	151.9	3	0.0294881
3	4.638	182	15.9	0.610966	3	3.86718	14.1	10.7	4.638	181.7	3.5	0.630188
4	4.91	219	19.2	0.0122008	1	0	0	0	4.91	215	1	0
5	5.005	232	20.3	0.109306	10	2.58141	9.4	7.1	5.005	237.3	10.4	0.139794
6	5.145	251	22	0.0683002	5	0.534119	1.9	1.5	5.145	254.1	5.2	0.0582486
7	5.864	419	36.7	0.5463	3	2.64822	9.7	7.3	5.864	419	2.8	0.537543
8	5.914	434	38	0.159586	3	0.906376	3.3	2.5	5.914	434.1	3.3	0.156986
9	5.972	451	39.5	0.355792	4	2.44746	8.9	6.8	5.972	450.7	4.1	0.332755
10	6.07	480	42	0.109963	3	0.618193	2.3	1.7	6.07	479.8	3.4	0.102132
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.097	488	42.7	0.0957429	2	0.391325	1.4	1.1	6.097	487.9	2.3	0.094479
12	6.138	500	43.8	0.538491	3	2.65678	9.7	7.3	6.138	499.2	2.9	0.515507
13	6.314	552	48.3	0.0347929	10	0.568422	2.1	1.6	6.314	556.2	9.7	0.0330516
14	6.379	571	50	0.146355	5	1.14332	4.2	3.2	6.379	571.2	5	0.129763
15	6.467	597	52.3	0.0638911	3	0.27755	1	0.8	6.467	596.7	2.8	0.0552981
16	6.508	609	53.3	0.119399	8	1.45183	5.3	4	6.508	610.8	8.4	0.0974026
17	7.328	748	65.5	0.0249824	3	0.129073	0.5	0.4	7.328	748.2	3	0.0239973
18	7.529	787	68.9	0.0625895	14	1.37873	5	3.8	7.529	790.4	13.5	0.0574245
19	7.617	804	70.4	0.280311	3	1.58351	5.8	4.4	7.617	803.6	3.5	0.258304
20	8.45	971	85	0.104105	16	2.84429	10.4	7.9	8.45	969.6	16.1	0.0999515

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	23	2	0.665383	4	4.93399	20.8	14	3.5	24.2	4.4	0.636777
2	4.55	169	14.8	0.322623	4	2.10323	8.9	6	4.55	169.3	4.1	0.291341
3	5.2	257	22.5	0.401981	4	2.65775	11.2	7.5	5.2	256.5	3.8	0.394025
4	5.85	413	36.2	0.256649	10	4.20262	17.7	11.9	5.85	407.4	10.3	0.230791
5	6.55	625	54.8	0.329546	4	2.39558	10.1	6.8	6.55	624.1	4.3	0.314392
6	6.85	661	57.9	0.155503	6	1.42627	6	4	6.85	660.5	5.5	0.145971
7	7.35	752	65.9	0.333748	5	3.01938	12.7	8.5	7.35	751.2	5.2	0.328213
8	8.15	905	79.3	0.0619038	17	1.79062	7.6	5.1	8.15	904.5	17	0.0592782
9	8.45	968	84.8	0.0610408	7	0.818748	3.5	2.3	8.45	969.4	7.5	0.0617497
10	8.65	1001	87.7	0.0323122	7	0.3656	1.5	1	8.65	1000.3	6.7	0.0307149

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 120

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 19.600 4.550 9.700 5.200 11.000 5.850 15.600 6.550 11.900 6.850 6.900 7.350 14.500 8.150 5.300 8.450 3.900 8.650 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 11 PeakCount: 10 Return to Lane Navigator
3.500 8.700 4.159 3.900 4.440 1.200 4.550 4.100 4.650 9.900 4.921 0 5.014 5.300 5.200 7.000 5.850 6.600 5.919 1.400 5.992 3.000 6.088 1.500 6.147 8.700 6.329 2.100 6.405 4.200 6.550 6.800 6.850 2.500 7.350 7.900 7.538 3.000 7.643 4.300 8.150 1.400 8.450 5.200 8.650 1.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 11 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 12 PeakCount: 23 Return to Lane Navigator
4.131 4.700 4.420 0.700 4.629 14.800 4.911 0 5.005 8.900 5.142 2.000 5.862 10.000 5.923 0.300 5.971 7.500 6.012 5.500 6.070 1.400 6.098 1.000 6.135 8.700 6.313 0.800 6.378 3.900 6.466 1.100 6.504 5.500 7.339 0.500 7.536 8.300 7.617 5.600 8.438 8.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 13 PeakCount: 21 Return to Lane Navigator
3.500 20.200 4.550 9.000 5.200 12.500 5.850 14.000 6.550 10.300 6.850 8.000 7.350 14.000 8.150 6.000 8.450 4.400 8.650 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 14 PeakCount: 10 Return to Lane Navigator
3.500 10.300 4.153 3.600 4.436 0.900 4.550 3.400 4.652 8.600 4.930 0.400 5.017 7.300 5.200 4.500 5.850 7.700 5.909 4.000 5.958 6.000 6.031 0.500 6.069 1.000 6.097 0.800 6.139 6.800 6.324 0.400 6.390 1.700 6.505 1.800 6.550 4.800 6.850 2.900 7.350 7.500 7.561 2.700 7.637 4.100 8.150 2.300 8.450 4.800 8.650 1.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 15 PeakCount: 26 Return to Lane Navigator
4.136 4.400 4.421 0.600 4.638 14.100 4.910 0 5.005 9.400 5.145 1.900 5.864 9.700 5.914 3.300 5.972 8.900 6.070 2.300 6.097 1.400 6.138 9.700 6.314 2.100 6.379 4.200 6.467 1.000 6.508 5.300 7.328 0.500 7.529 5.000 7.617 5.800 8.450 10.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 16 PeakCount: 20 Return to Lane Navigator
3.500 20.800 4.550 8.900 5.200 11.200 5.850 17.700 6.550 10.100 6.850 6.000 7.350 12.700 8.150 7.600 8.450 3.500 8.650 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Canary Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish ---- Go to Display Gel Lane Navigator.