RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Striped Marlin ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Tetrapturus audax
FDA Seafood List Common Name: Striped Marlin
FDA Seafood List Market Name: Marlin
Unique IEF Gel Code: 19970212gel04
Unique Web Code: smh01Srr
Tissue Aliquot Code: tetrauda I96-002 #07 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	21	2.8	0.0441018	4	0.271375	3	2.3	4.55	21.5	3.5	0.0432951
2	5.2	113	14.8	0.0773471	4	0.579675	6.4	4.9	5.2	113.5	4.4	0.0739371
3	5.85	231	30.3	0.140147	4	0.892681	9.9	7.5	5.85	231.2	3.6	0.141488
4	6.55	372	48.8	0.148574	7	1.59107	17.7	13.4	6.55	371.1	6.5	0.137089
5	6.85	404	53	0.0525068	5	0.415182	4.6	3.5	6.85	404.6	4.7	0.049329
6	7.35	469	61.5	0.0873312	4	0.665644	7.4	5.6	7.35	468.9	4.4	0.0846879
7	8.15	582	76.4	0.118243	7	1.3159	14.6	11.1	8.15	581.4	6.6	0.112382
8	8.45	648	85	0.117293	9	1.77371	19.7	14.9	8.45	645.5	8.7	0.115269
9	8.65	697	91.5	0.0967911	4	0.731209	8.1	6.1	8.65	696.1	4.4	0.0946846
10	9.3	749	98.3	0.0496782	11	0.758699	8.4	6.4	9.3	745.4	10.8	0.0396542

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.172	107	14	0.00972202	3	0.0551477	0.1	0.1	5.172	106.7	3.4	0.00926876
2	5.238	118	15.5	0.00813141	5	0.12847	0.3	0.3	5.238	121.9	4.8	0.0150973
3	5.809	222	29.1	0.0108212	4	0.0895813	0.2	0.2	5.809	219.8	4.4	0.0114639
4	5.882	236	31	0.0319379	3	0.178044	0.4	0.4	5.882	236.4	3.4	0.0296124
5	6.199	300	39.4	0.0535061	6	0.594244	1.3	1.3	6.199	301.6	6.4	0.0521164
6	6.327	326	42.8	0.59111	3	2.95498	6.5	6.3	6.327	326.3	3	0.562964
7	6.416	344	45.1	0.222444	7	2.87316	6.4	6.1	6.416	346.2	7.1	0.228607
8	6.451	351	46.1	0.220487	2	0.258299	0.6	0.6	6.451	351.6	2	0.0717278
9	6.515	364	47.8	0.0327545	3	0.113765	0.3	0.2	6.515	364.6	2.6	0.0246883
10	6.632	380	49.9	0.0158338	2	0.0545101	0.1	0.1	6.632	379.5	1.8	0.0166246
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.723	390	51.2	0.0400799	3	0.207854	0.5	0.4	6.723	389.4	3.3	0.0358099
12	6.823	401	52.6	0.0407143	2	0.130074	0.3	0.3	6.823	401.2	1.7	0.0420001
13	6.95	417	54.7	0.252134	2	0.952735	2.1	2	6.95	417	2.3	0.231459
14	7.004	424	55.6	0.0838045	6	0.827814	1.8	1.8	7.004	424	6.4	0.0724475
15	7.088	435	57.1	0.460676	3	2.27627	5	4.9	7.088	434.9	3	0.426102
16	7.235	454	59.6	0.554858	3	3.3231	7.4	7.1	7.235	453.5	3.5	0.538251
17	7.327	466	61.2	0.176756	2	0.707702	1.6	1.5	7.327	466.4	2.2	0.181919
18	7.551	497	65.2	0.0104896	3	0.0580187	0.1	0.1	7.551	497	3	0.0109968
19	7.702	518	68	0.142364	18	5.81679	12.9	12.4	7.702	528.7	18	0.182324
20	7.967	555	72.8	0.402533	12	7.41528	16.4	15.9	7.967	551	11.8	0.353387
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.132	578	75.9	0.267341	7	3.57556	7.9	7.7	8.132	577.5	7.4	0.271179
22	8.405	635	83.3	0.0350642	4	0.233529	0.5	0.5	8.405	634.8	3.6	0.0365334
23	8.456	646	84.8	0.020923	3	0.107657	0.2	0.2	8.456	645.2	2.8	0.0213686
24	8.499	657	86.2	0.0869322	2	0.361554	0.8	0.8	8.499	656.7	2.3	0.0885887
25	8.565	674	88.5	0.417009	3	2.37828	5.3	5.1	8.565	674	3.2	0.418585
26	8.738	703	92.3	0.165539	13	3.26122	7.2	7	8.738	701.2	12.8	0.143506
27	9.003	724	95	0.182029	14	4.51617	10	9.7	9.003	723.4	14.4	0.177291
28	9.231	742	97.4	0.176357	7	1.67236	3.7	3.6	9.231	742.5	6.8	0.139672

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	18	2.4	0.0387906	4	0.280042	3.9	2.9	4.55	18.4	4.3	0.036355
2	5.2	109	14.3	0.0700481	5	0.551852	7.7	5.7	5.2	109.2	4.6	0.0671814
3	5.85	228	29.9	0.124226	4	0.852381	11.9	8.8	5.85	228.1	3.9	0.123717
4	6.55	370	48.6	0.108402	7	1.17327	16.3	12.2	6.55	369.3	6.5	0.101274
5	6.85	404	53	0.0362944	4	0.264518	3.7	2.7	6.85	404.7	4.4	0.0339794
6	7.35	469	61.5	0.0658215	5	0.519623	7.2	5.4	7.35	469.3	4.6	0.0635069
7	8.15	579	76	0.0882122	8	1.23412	17.2	12.8	8.15	579	8.3	0.0836908
8	8.45	641	84.1	0.124554	7	1.54473	21.5	16	8.45	640.9	7.1	0.122535
9	8.65	695	91.2	0.0878276	4	0.613429	8.5	6.4	8.65	694.2	4	0.0867835
10	9.3	746	97.9	0.0313013	3	0.148645	2.1	1.5	9.3	745.7	2.6	0.0321805

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	27	3.5	0.0427065	4	0.272129	0.9	0.8	4.55	26.5	3.6	0.0425317
2	4.735	54	7.1	0.0147786	4	0.102802	0.3	0.3	4.735	54.8	4.4	0.0133108
3	4.837	69	9.1	0.00943237	5	0.0946423	0.3	0.3	4.837	71.1	4.9	0.0108001
4	5.2	122	16	0.0767154	5	0.633696	2.1	1.9	5.2	122.3	5	0.0721099
5	5.85	234	30.7	0.107488	4	0.807914	2.7	2.4	5.85	234.5	4.4	0.1043
6	6.205	302	39.6	0.0290778	6	0.338674	1.1	1	6.205	303.5	6.5	0.0295924
7	6.336	327	42.9	0.521921	3	2.63116	8.9	8	6.336	326.7	2.9	0.510789
8	6.435	346	45.4	0.177501	6	1.90456	6.4	5.8	6.435	345.6	6.1	0.177318
9	6.461	351	46.1	0.164927	2	0.414535	1.4	1.3	6.461	351.6	2.5	0.0945975
10	6.55	368	48.3	0.0491124	5	0.470568	1.6	1.4	6.55	367.2	5.2	0.0511307
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.743	391	51.3	0.0209529	3	0.0969321	0.3	0.3	6.743	391.3	2.9	0.0185853
12	6.844	403	52.9	0.0782369	2	0.30458	1	0.9	6.844	403.2	2.1	0.0805106
13	6.907	411	53.9	0.0149375	1	0.0453952	0.2	0.1	6.907	410.2	1.4	0.0177824
14	6.977	420	55.1	0.189152	3	1.03457	3.5	3.1	6.977	420.1	3.2	0.182187
15	7.109	437	57.3	0.426396	3	2.05472	7	6.2	7.109	437.2	2.9	0.398945
16	7.249	455	59.7	0.521901	3	2.95493	10	8.9	7.249	455.4	3.2	0.527169
17	7.35	468	61.4	0.183289	3	0.880991	3	2.7	7.35	467.8	2.6	0.189489
18	7.55	496	65.1	0.0305709	3	0.164424	0.6	0.5	7.55	496	3	0.0309222
19	7.714	519	68.1	0.12539	18	4.62337	15.6	14	7.714	532.7	18	0.144917
20	8.021	562	73.8	0.0755814	8	1.51874	5.1	4.6	8.021	547.1	8.2	0.104509
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.15	580	76.1	0.223134	5	2.10283	7.1	6.4	8.15	580.5	5.2	0.226029
22	8.383	632	82.9	0.0188765	2	0.0331312	0.1	0.1	8.383	631.6	1.7	0.0112171
23	8.45	647	84.9	0.08264	7	1.23426	4.2	3.7	8.45	649.7	7.2	0.0973202
24	8.548	672	88.2	0.428121	3	2.18563	7.4	6.6	8.548	671.8	2.8	0.433256
25	8.65	698	91.6	0.0931891	9	1.29682	4.4	3.9	8.65	697.8	9.2	0.0796691
26	9.051	727	95.4	0.0292543	13	0.71436	2.4	2.2	9.051	719.3	13.3	0.030412
27	9.259	742	97.4	0.0746957	5	0.630486	2.1	1.9	9.259	742.2	5	0.0710437

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.695	46	6	0.0142193	4	0.0898046	0.2	0.2	4.695	45.6	3.8	0.0134691
2	4.785	59	7.7	0.00693392	4	0.0465732	0.1	0.1	4.785	59.6	3.6	0.0072348
3	4.923	79	10.3	0.0113776	8	0.14102	0.3	0.3	4.923	79.2	7.7	0.0103264
4	5.159	113	14.8	0.0161101	6	0.15737	0.4	0.4	5.159	114.4	5.7	0.0154863
5	5.735	212	27.7	0.0154066	3	0.0829328	0.2	0.2	5.735	212.6	3	0.0156143
6	5.821	227	29.7	0.0293719	4	0.209011	0.5	0.5	5.821	228	4.1	0.0285016
7	6.166	295	38.6	0.0574323	7	0.70817	1.7	1.7	6.166	295.9	6.9	0.0582447
8	6.297	321	42	0.554887	3	2.84023	6.9	6.6	6.297	321.1	2.9	0.545921
9	6.392	340	44.5	0.206393	5	1.94811	4.7	4.6	6.392	341.7	5	0.22181
10	6.427	347	45.4	0.217999	2	0.708876	1.7	1.7	6.427	347.8	2.4	0.165723
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.492	360	47.1	0.0535851	3	0.342341	0.8	0.8	6.492	360.3	3.5	0.0559256
12	6.59	376	49.2	0.0187952	2	0.065388	0.2	0.2	6.59	376.3	1.9	0.0198326
13	6.687	387	50.7	0.045796	3	0.215234	0.5	0.5	6.687	387	3.1	0.0396589
14	6.794	399	52.2	0.041213	2	0.137662	0.3	0.3	6.794	398.4	1.5	0.0508745
15	6.847	405	53	0.0268649	2	0.101344	0.2	0.2	6.847	405.4	1.9	0.0293987
16	6.925	415	54.3	0.24071	3	1.09961	2.7	2.6	6.925	414.8	2.6	0.23571
17	6.979	422	55.2	0.0848748	5	0.702036	1.7	1.6	6.979	422	5	0.0789647
18	7.064	433	56.7	0.410375	3	2.26805	5.5	5.3	7.064	432.8	3.4	0.380259
19	7.211	452	59.2	0.530643	4	3.32479	8.1	7.8	7.211	451.8	3.8	0.49453
20	7.311	465	60.9	0.175411	2	0.686947	1.7	1.6	7.311	465.1	2.2	0.179428
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.546	498	65.2	0.0183643	5	0.13818	0.3	0.3	7.546	497.4	4.5	0.0173092
22	7.708	521	68.2	0.126479	17	4.06746	9.9	9.5	7.708	528.7	16.8	0.136343
23	7.968	558	73	0.328961	13	7.0931	17.3	16.6	7.968	552.2	13.3	0.300875
24	8.122	580	75.9	0.210906	7	2.33829	5.7	5.5	8.122	581.8	7	0.18976
25	8.418	642	84	0.0315924	3	0.112831	0.3	0.3	8.418	641.4	2.6	0.0246084
26	8.454	650	85.1	0.0579116	7	0.700269	1.7	1.6	8.454	651.6	6.8	0.0577514
27	8.494	660	86.4	0.0887038	2	0.349555	0.9	0.8	8.494	660.8	2.4	0.0814624
28	8.566	678	88.7	0.459654	3	2.23281	5.4	5.2	8.566	677.8	2.8	0.455796
29	8.747	706	92.4	0.141052	15	3.86017	9.4	9	8.747	707.9	15.5	0.140616
30	9.079	730	95.5	0.158794	9	2.15721	5.2	5	9.079	730	9.2	0.132195
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.259	743	97.3	0.208128	7	2.18018	5.3	5.1	9.259	743.6	6.5	0.187866

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	21	2.7	0.0643276	4	0.431085	4	3	4.55	20.7	3.8	0.0631745
2	5.2	114	14.9	0.115576	4	0.799459	7.4	5.6	5.2	113.9	4.1	0.109952
3	5.85	228	29.8	0.157666	4	1.17635	10.9	8.2	5.85	227.8	4.2	0.156758
4	6.55	373	48.8	0.155747	7	1.81287	16.9	12.6	6.55	371.9	7.1	0.144841
5	6.85	405	53	0.0579075	5	0.482016	4.5	3.4	6.85	405.7	5.1	0.05331
6	7.35	470	61.5	0.0969907	5	0.820274	7.6	5.7	7.35	470.2	5	0.0928753
7	8.15	586	76.7	0.137378	8	1.75541	16.3	12.2	8.15	586	7.5	0.131971
8	8.45	649	84.9	0.12588	9	1.89253	17.6	13.2	8.45	648.9	8.7	0.122766
9	8.65	698	91.4	0.104064	5	0.887106	8.3	6.2	8.65	698.1	4.9	0.10232
10	9.3	745	97.5	0.0639976	7	0.686307	6.4	4.8	9.3	743.4	6.9	0.0563161

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 116

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 3.000 5.200 6.400 5.850 9.900 6.550 17.700 6.850 4.600 7.350 7.400 8.150 14.600 8.450 19.700 8.650 8.100 9.300 8.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
5.172 0.100 5.238 0.300 5.809 0.200 5.882 0.400 6.199 1.300 6.327 6.500 6.416 6.400 6.451 0.600 6.515 0.300 6.632 0.100 6.723 0.500 6.823 0.300 6.950 2.100 7.004 1.800 7.088 5.000 7.235 7.400 7.327 1.600 7.551 0.100 7.702 12.900 7.967 16.400 8.132 7.900 8.405 0.500 8.456 0.200 8.499 0.800 8.565 5.300 8.738 7.200 9.003 10.000 9.231 3.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 28 Return to Lane Navigator
4.550 3.900 5.200 7.700 5.850 11.900 6.550 16.300 6.850 3.700 7.350 7.200 8.150 17.200 8.450 21.500 8.650 8.500 9.300 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 0.900 4.735 0.300 4.837 0.300 5.200 2.100 5.850 2.700 6.205 1.100 6.336 8.900 6.435 6.400 6.461 1.400 6.550 1.600 6.743 0.300 6.844 1.000 6.907 0.200 6.977 3.500 7.109 7.000 7.249 10.000 7.350 3.000 7.550 0.600 7.714 15.600 8.021 5.100 8.150 7.100 8.383 0.100 8.450 4.200 8.548 7.400 8.650 4.400 9.051 2.400 9.259 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator
4.695 0.200 4.785 0.100 4.923 0.300 5.159 0.400 5.735 0.200 5.821 0.500 6.166 1.700 6.297 6.900 6.392 4.700 6.427 1.700 6.492 0.800 6.590 0.200 6.687 0.500 6.794 0.300 6.847 0.200 6.925 2.700 6.979 1.700 7.064 5.500 7.211 8.100 7.311 1.700 7.546 0.300 7.708 9.900 7.968 17.300 8.122 5.700 8.418 0.300 8.454 1.700 8.494 0.900 8.566 5.400 8.747 9.400 9.079 5.200 9.259 5.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 31 Return to Lane Navigator
4.550 4.000 5.200 7.400 5.850 10.900 6.550 16.900 6.850 4.500 7.350 7.600 8.150 16.300 8.450 17.600 8.650 8.300 9.300 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Striped Marlin ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Striped Marlin ---- Go to Display Gel Lane Navigator.