Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Streaked Spinefoot  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Siganus javus
FDA Seafood List Common Name: Streaked Spinefoot
FDA Seafood List Market Name: Spinefoot
Unique IEF Gel Code: 19971029gel04
Unique Web Code: rbh01Srr
Tissue Aliquot Code: sigajavu I96-002 #02m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5594110.1276840.87779210.47.84.5594.44.20.117736
25.218421.50.11835340.7888069.475.21844.10.10968
35.8530135.20.14043630.86059110.27.65.85301.33.50.138904
46.5544852.40.16093761.547318.413.76.554475.80.150794
56.8548156.30.032960570.3238983.92.96.85480.97.20.0254827
67.3555364.70.062220150.4999825.94.47.35551.94.90.0576826
78.1567979.40.04535290.715528.56.38.15677.490.0449966
88.4574186.70.099104561.0166212.198.45741.760.0953922
98.6578992.30.082002840.5429326.54.88.65788.740.0765158
109.383597.70.14584151.2390814.7119.3834.25.20.135276
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.968202.30.10429461.139849.88.53.96819.96.40.0999253
24.134424.90.075705850.6409785.54.84.13442.44.70.0772106
34.87514016.40.010185550.07069070.60.54.875139.14.60.0085808
45.02616018.70.015084750.11431710.85.0261614.90.013255
55.31620423.80.007123130.04357740.40.35.316203.33.40.0073257
65.49723727.70.08206950.675545.855.497236.84.50.08386
75.53624428.50.055488830.2714582.325.536244.23.10.0489546
85.56424929.10.039837930.1721011.51.35.564249.82.60.0376416
95.61325830.10.009838520.0368860.30.35.613257.92.10.0098419
106.04934340.10.015179830.07359420.60.56.0493432.70.0156349
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.211377440.010681820.03436880.30.36.211377.31.80.0110394
126.249385450.006160120.02611520.20.26.249384.62.10.0069529
136.28239245.80.008528320.02105250.20.26.282391.81.60.0074324
146.329402470.008848130.04152110.40.36.32940230.0078899
156.37741248.10.045421230.2230241.91.76.377411.630.0424214
166.40541848.80.096094630.5315044.53.96.405417.53.10.0952225
176.43842549.60.149720.5220044.53.96.438424.92.40.124986
186.49143650.90.049250870.7719666.65.76.491432.47.40.0590414
196.59145352.90.56067532.9637525.4226.591452.830.562008
206.72746854.70.093966740.633055.44.76.727468.94.10.0864283
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.83648056.10.23823531.2300610.59.16.836480.93.10.22689
226.93749457.70.031069720.11422610.86.937494.32.10.0311256
237.12452160.90.24233131.3369711.49.97.124520.43.10.240716
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5510211.90.12963661.1680111.28.34.55102.95.80.114396
25.218421.40.10902750.843748.165.2184.54.70.101002
35.8530335.20.16473841.107310.77.95.85303.43.90.161411
46.5545152.40.18914661.7385216.712.46.55450.55.50.177191
56.8548456.30.031875880.3452643.32.56.85484.27.80.0251172
67.3555664.70.056920650.4314774.23.17.355564.60.0526679
78.1568379.40.0423848130.9217488.96.68.15685.413.40.0388113
88.4574486.50.13648371.6543615.911.88.45744.87.20.130295
98.6579392.20.1127640.8484998.268.65792.74.40.109633
109.383797.30.1562351.3360512.99.59.3836.75.30.141244
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.017313.60.1133871.362086.65.74.017327.40.103411
24.185546.30.098962760.931034.53.94.18553.75.50.0949637
34.5510412.10.094538840.63002232.74.55104.340.0888421
44.857146170.018769970.21217410.94.857145.56.80.0177121
55.03917119.90.013837450.1062440.50.45.039171.24.60.0130212
65.219322.50.079941540.6033432.92.55.2192.84.40.0770015
75.33421725.30.010994640.07516980.40.35.334216.24.30.0098969
85.53125229.30.11688181.601337.76.85.5312518.10.111173
95.564258300.10687140.3619261.71.55.564258.94.30.0479525
105.60326530.80.089872540.5010072.42.15.603264.54.20.0677546
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.64327231.70.064567360.641543.12.75.643272.360.0605794
125.85309360.092568240.6074632.92.65.85309.73.70.0922582
136.06935441.20.01862830.09960050.50.46.069354.43.50.0162587
146.22538644.90.010914580.1256540.60.56.225383.78.20.0086946
156.29840146.70.008597710.01623020.10.16.298400.81.40.0066803
166.38641948.80.067471181.066075.14.56.386422.880.074862
176.4242649.60.13053730.2749231.31.26.42426.42.60.0598352
186.45443350.40.18444130.5245692.52.26.454433.22.60.114204
196.49344151.30.105418101.944399.48.26.493441.610.30.106719
206.63746253.80.48973832.5535612.310.86.637462.130.482574
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.77647755.50.086365440.62658832.66.776477.74.30.0829772
226.87148856.80.26009431.528657.46.46.871488.73.40.253455
236.96350158.30.031357920.10120.50.46.963501.31.80.031273
247.13952661.20.24212731.311326.35.57.139525.63.10.236353
257.3555664.70.047698580.6455123.12.77.35555.88.30.0439456
268.1568980.20.025627110.4331472.11.88.15688.511.10.022071
278.4574987.20.071329760.765953.73.28.45749.26.40.0671814
288.6579592.50.050365540.3268131.61.48.65794.73.80.0490783
299.383797.40.077274570.802243.93.49.3835.17.30.0623988
PeakCounter: 29
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.973242.80.12170571.4628811.4103.97324.570.118592
24.14475.50.076400660.8185286.45.64.1447.26.10.0757511
34.83714316.60.0073833110.1359021.10.94.837139.811.40.0067019
45.01816819.50.011615560.1035470.80.75.018167.55.90.0098398
55.29421024.40.009628940.05204110.40.45.294209.93.80.0077199
65.49424628.60.090265281.27635108.75.49424680.0902418
75.53325329.40.079013630.191171.51.35.533253.93.50.030857
85.57226030.20.066277140.3743772.92.55.572258.94.40.0483404
95.611267310.051839170.5701524.53.95.6112676.60.0488764
106.02734740.30.01736840.09335550.70.66.027347.13.50.01485
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.20438444.60.010051930.04387310.30.36.204383.53.30.0076156
126.24239245.50.004427910.01030970.10.16.242391.81.30.0044805
136.28140046.50.00430720.01534690.10.16.281399.82.20.0040255
146.31940847.40.006708240.04265410.30.36.319409.43.90.0061399
156.37642048.80.034379630.2219861.71.56.376420.73.40.036535
166.4142749.60.07833830.4090173.22.86.41426.930.0763815
176.44843550.50.11928730.4735773.73.26.448434.82.50.106275
186.49644551.70.042633970.6263084.94.36.496442.36.70.052781
196.60446253.70.5316932.8378222.219.36.604461.930.53372
206.74647755.40.078244640.5563254.33.86.746477.340.0777863
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.85748956.80.19222731.123168.87.66.857489.33.40.187566
226.95150258.30.020212120.09043690.70.66.951502.62.50.0206593
237.116525610.19933641.268229.98.67.116524.43.60.200949
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5510311.90.1340561.26611.48.64.55103.76.10.116245
25.219322.30.13363851.0031496.85.21934.60.122729
35.85311360.18050741.09659.87.45.85310.83.50.174928
46.5546053.20.21580861.9529217.513.36.55459.45.50.199038
56.8549156.80.036213180.3915243.52.76.85491.77.70.0288083
67.3555964.60.055679640.4004053.62.77.35558.34.40.051829
78.1568479.10.0622884141.3591712.29.28.1568514.30.0535406
88.4575186.80.14097371.776515.912.18.45751.57.30.137384
98.6580192.60.11039640.7577446.85.18.65800.340.105805
109.384397.50.14775651.1404810.27.79.38424.70.137922
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 105
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 29 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln