CFSAN Regulatory Codes: 1,3,5,8
CFSAN Program Priority Codes: 1.6A
Start Date: 5/1/98    Completion Date: 9/30/03

Statement of Research Problem:
Within the broad scope of research projects conducted under the FSI, there is often a need to occasionally access specialized equipment or expertise to complete or advance a research project. It is therefore essential to the research program that there be access to this expertise/technology so research projects can be completed in a cost-effective, timely manner.

Statement of Project Objective(s):
The purpose of this project is to provide all FSI research projects with a cost-effective means of accessing high cost, specialized instrumentation and skilled researchers. This group includes personnel with specialized knowledge in the preparation of biological specimens, handling of animals and pathology services, as well as, key personnel for the operation of highly technical instruments. Access to this type of state-of-the-art technology permits development and refinement of detection, identification and quantification methods.

Anticipated Impact on FDA Regulatory Program:

1. Development of new analytical methods.
2. Identification of new biomarkers for exposure, virulence, risk assessment, etc.

Project Priority Changes During FY2000: none

Collaborators: Numerous investigators from virtually all FSI projects

1. Develop protocols and methods
2. Initiate and evaluate new methods or sample analysis techniques
3. Analyze samples and review protocols in support of other FSI research projects
4. Provide animal handling and pathology services

1. Publish methods and protocols
2. Present data at meetings and other public forums
3. Serve as a clearinghouse for experimental data obtained in support role

1. Published or submitted 15 articles to peer reviewed journals
2. Attended and provided abstracts for 21 scientific meetings
3. Completed ribotyping of outbreak associated strains of Vibrio parahaemolyticus and Salmonella isolates.
4. Completed 24 electron microscopy studies involving 201 specimens received from nine investigators.

1. Publish methods and protocols
2. Present data at meetings and other public forums
3. Serve as a clearinghouse for experimental data obtained in support role
4. Complete studies

1. Publish methods and protocols
2. Present data at meetings and other public forums
3. Serve as a clearinghouse for experimental data obtained in support role
4. Complete studies

Roach, J.A.G., White, K.D., Trucksess, M.W., Thomas, F.W., Capillary GC/MS with chemical ionization and negative ion detection for the confirmation of identity of patulin in apple juice, Journal of AOAC International, (2000) 83, 104-112.

Hartman, G., D. Stewart, and S. Gendel. 2000. Identification of Listeria monocytogenes from Ice Cream Using the BAX System. Laboratory Information Bulletin 4213, 1-7

Gendel, S. and J. Ulaszek. 2000. Ribotype Analysis of Strain Distribution in Listeria monocytogenes. Journal of Food Protection 63, 179-185.

Hood, H.L., Kraeling, M.E.K., Robl, M.G. and Bronaugh, R.L. The Effects of Alpha Hydroxy Acid (glycolic Acid) on Hairless Guinea Pig Skin Permeability. Food and Chemical Toxicology 37:1105-1111, 1999.

Eberhard, M., Ortega, Y.R., Hanes, D.E. Nace, E.K. Do, R.Q., Robl, M.G., Won, K.Y., Gavidia, C., Sass, N.L., Mansfield, K., Gonzalo, A., Griffiths, J., Gilman, R., Sterling, C.R. and Arrowood, M.J. Attempts to Establish Experimental Cyclospora cayetanesis Infection in Laboratory Animals. J. Parasitol. 86(3):577-582, 2000.

Principato, M.A., Grenade, C., Weaver, J., Osborne, R. Robl, M.G. Tissue Specific Immune Responsiveness to Staphylococcal Eneterotoxin in the Aged Gut Lymphatics. FESEB, 1999.

Dickey, R.W., Jester, E.L.E., Granade, H.R., Mowdy, D.E., Moncrief, C., Rebarchik, D., Robl, M. and Musser, S. M. Monitoring Shellfish Toxicity during a Gymnodinium breve Red Tide: Comparison of Sodium Channel Specific Cytotoxicity Assay and Mouse Bioassay for Determination of Neurotoxic Shellfish Toxins in Shellfish Extracts. Nat. Tox., 7:157-165, 1999.

Poli, M.A., Musser, S. M., Dickey, R.W., Eilers, P. P. and Hall, S. Neurotoxic Shellfish Poisoning And Brevetoxin Metabolites: Case Study From Florida, Toxicon, 38:981-993, 2000.