We compared the acceptance of virgin and mated queens introduced into queenless hives using either artificial queen cells or direct introduction accompanied by smoke.  In Experiment 1, virgin queens aged 3-4 days were introduced into 5-frame hives than had been dequeened 1, 2, 3, 4, 5, or 6 days previously.  Acceptance increased significantly with the length of time a colony had been queenless, and direct introduction gave significantly greater success than artificial queen cells (between 31% and 100% acceptance vs. 8% to 92% for cell or direct introduction respectively, depending on the period of queenlessness).  In Experiment 2, virgin and mated queens were introduced into 2-frame observation hives that had been dequeened 1, 2, 3 and 4 days previously.  The probability of acceptance was significantly higher for mated queens than virgins, for direct introduction versus artificial queen cells, and for longer queenless periods.  Accordingly, the probability of a queen being balled by the workers declined significantly with the duration of the queenless period, and was significantly less for mated versus virgin queens.  Finally, in Experiment 3, we introduced mated queens into medium-sized hives (10 medium Langstroth frames) that had been queenless for 2 days using both the direct introduction and artificial cells.  All queens were accepted.

Apis mellifera mellifera, virgin queen, mated queen, queen introduction, queen rearing, queen balling, observation hive, artificial queen cell