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MGC ESTs
The initial step in the identification of a full-length ORF clone from
an MGC cDNA library is 5' EST sequencing. (Originally, one 384 well plate per library was also submitted for 3' sequencing but
this practice was soon discontinued.) All these sequences are deposited in GenBank and are found at:
The sequence of each 5' EST is analyzed, and if it meets selected criteria (described in
the PNAS paper "Generation
and Initial Analysis of more than 15,000 Full-Length Human
and Mouse cDNA Sequences"), and if it is also unique, it is designated as a "Candidate
Clone". It is then
returned to the MGC pipeline for full insert sequencing and reanalysis to confirm the presence of a complete open reading frame.
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