Detailed Research Information

Detailed project information for
Study Plan Number 08050

Branch : Northern Appalachian Research Laboratory

Study Plan Number : 08050

Study Title : Application of calcein as a dietary component for fish marking to enhance product evaluation and management capabilities

Starting Date : 06/01/2005

Completion Date : 09/30/2007

Principal Investigator(s) : Honeyfield, Dale C.

Primary PI : Honeyfield, Dale C.

Telephone Number : (570) 724-3322 ext.

Email Address : honeyfie@usgs.gov

SIS Number :

Primary Program Element :

Second Program Element :

Status : Active

Abstract : BACKGROUND
The principle client of the work is the USFWS. In addition, NOAA, Great Lakes Fishery Commission Fish Health committee and individual state agencies have expressed interest in the development of better non-antibiotic chemical marking agent. Currently Ontario Ministry of Natural Resources prohibits the use of tetracycline, a common otilith marking agent. State agencies have also expressed concern about the use of an antibiotic in all released fish. This work addresses stated needs of the USFWS. In 2004 the FWS identified calcein as one of three fisheries operation needs as a national project at the annual Fisheries Technology Centers meeting. The proposed studies to investigate marking of aquatic trust species are within the goals of the Fisheries and Aquatic Resources program of Biological Resources Division (BRD) and are consistent with Leetown Science Center’s research direction.

OBJECTIVES

The overall objective of the study is to develop adequate background information regarding calcein administration via feed to justify pursuit of an INAD for oral administration of calcein as a non-lethal marking agent. Once approved, the development of calcein mark induction techniques for trust species will enable managers to conduct hatchery product evaluations and population monitoring studies:

1. Determine the scale fluorescence luminosity in fish fed dietary calcein;

a. Determine scale mark luminosity in brook trout fed at the rate of 2 % body weight per day for five days dietary calcein powder (0.0 to 7.50 g calcein/kg feed) combined into a commercial diet.

b. Determine scale mark luminosity in brook trout fed dietary encapsulated calcein (0.0 to 7.50 g calcein/kg feed) combined into a commercial diet.

c. Determine if there is a difference in scale mark luminosity between brook trout and rainbow trout fed the similar concentrations of calcein.

2. Determine the effect of natural or simulated natural light intensity on longevity or retention of a visible calcein mark.

3. Based on the data generated in objective one and preliminary tests as needed for experimental species, evaluate longevity of scale or bony tissue mark luminosity in fish species of interest such as lake trout, brook trout, Atlantic salmon, striped bass (or alternate warm-water species), Snake River cutthroat trout and shovelnose sturgeon.

HYPOTHESIS TO BE TESTED

1. Feed ingredients in commercial feed will not affect the availability of calcein.

2. There is no difference in boney tissue marking with calcein incorporated directly into the feed as a powder or the inclusion of calcein as an encapsulated product.

3. Natural light intensity has no affect on longevity of calcein mark detection.

4. There are no differences in mark retention and/or effective dose rates among trust species fed calcein as a bony tissue marking agent.

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U.S. Department of the Interior || U.S. Geological Survey
11700 Leetown Road, Kearneysville, WV 25430, USA
URL: http://www.lsc.usgs.gov
Maintainer: lsc_webmaster@usgs.gov
Last Modified: October 21, 2002 dwn
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Branch :	Northern Appalachian Research Laboratory
Study Plan Number :	08050
Study Title :	Application of calcein as a dietary component for fish marking to enhance product evaluation and management capabilities
Starting Date :	06/01/2005
Completion Date :	09/30/2007
Principal Investigator(s) :	Honeyfield, Dale C.
Primary PI :	Honeyfield, Dale C.
Telephone Number :	(570) 724-3322 ext.
Email Address :	honeyfie@usgs.gov
SIS Number :
Primary Program Element :
Second Program Element :
Status :	Active
Abstract :	BACKGROUND The principle client of the work is the USFWS. In addition, NOAA, Great Lakes Fishery Commission Fish Health committee and individual state agencies have expressed interest in the development of better non-antibiotic chemical marking agent. Currently Ontario Ministry of Natural Resources prohibits the use of tetracycline, a common otilith marking agent. State agencies have also expressed concern about the use of an antibiotic in all released fish. This work addresses stated needs of the USFWS. In 2004 the FWS identified calcein as one of three fisheries operation needs as a national project at the annual Fisheries Technology Centers meeting. The proposed studies to investigate marking of aquatic trust species are within the goals of the Fisheries and Aquatic Resources program of Biological Resources Division (BRD) and are consistent with Leetown Science Center’s research direction. OBJECTIVES The overall objective of the study is to develop adequate background information regarding calcein administration via feed to justify pursuit of an INAD for oral administration of calcein as a non-lethal marking agent. Once approved, the development of calcein mark induction techniques for trust species will enable managers to conduct hatchery product evaluations and population monitoring studies: 1. Determine the scale fluorescence luminosity in fish fed dietary calcein; a. Determine scale mark luminosity in brook trout fed at the rate of 2 % body weight per day for five days dietary calcein powder (0.0 to 7.50 g calcein/kg feed) combined into a commercial diet. b. Determine scale mark luminosity in brook trout fed dietary encapsulated calcein (0.0 to 7.50 g calcein/kg feed) combined into a commercial diet. c. Determine if there is a difference in scale mark luminosity between brook trout and rainbow trout fed the similar concentrations of calcein. 2. Determine the effect of natural or simulated natural light intensity on longevity or retention of a visible calcein mark. 3. Based on the data generated in objective one and preliminary tests as needed for experimental species, evaluate longevity of scale or bony tissue mark luminosity in fish species of interest such as lake trout, brook trout, Atlantic salmon, striped bass (or alternate warm-water species), Snake River cutthroat trout and shovelnose sturgeon. HYPOTHESIS TO BE TESTED 1. Feed ingredients in commercial feed will not affect the availability of calcein. 2. There is no difference in boney tissue marking with calcein incorporated directly into the feed as a powder or the inclusion of calcein as an encapsulated product. 3. Natural light intensity has no affect on longevity of calcein mark detection. 4. There are no differences in mark retention and/or effective dose rates among trust species fed calcein as a bony tissue marking agent.
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U.S. Department of the Interior \|\| U.S. Geological Survey 11700 Leetown Road, Kearneysville, WV 25430, USA URL: http://www.lsc.usgs.gov Maintainer: lsc_webmaster@usgs.gov Last Modified: October 21, 2002 dwn Privacy Policy and Disclaimers \|\| FOIA \|\| Accessibility