![Microscopic and SEM images of Dinophysis](images/Dinophysis.jpg) |
Dinophysis sp. |
In Europe, the consumption of shellfish that have filtered cells
of the dinoflagellate genus Dinophysis have led to cases
of intestinal upset. Two varieties appear responsible for DSP:
Dinophysis acuta and D. acuminata. Because of
these symptoms, this syndrome has been designated as Diarrhetic
Shellfish Poisoning or DSP. DSP is a significant problem in northern
Spain, Ireland, and the Mediterranean/Adriatic Sea. The toxin
has been detected in shellfish in Eastern Canada. While no cases
of DSP have ever been reported along the West coast of the U.S.,
the organisms that cause this poisoning are commonly found in
British Columbia and Puget Sound in Washington State. Very recently
(in 2003), the toxins have been detected in shellfish from British
Columbia and are more than likely to be found wherever the organisms
are present.
The toxin in DSP appears to be okadaic acid and some related
compounds, the dinophysistoxins. The toxins are fat soluble, have
high molecular weights, and belong to a class of compounds called
polycyclic ethers. Interestingly, these compounds (see chemical
structures below) are somewhat similar in chemical structure to
the rather exotic marine toxins that are associated with Ciguatera
Fish Poisoning or Tropical Fish Poisoning in that the repeating
polyether moieties are present in these molecules.
While at first glance, diarrhea would appear to be a relatively
minor ailment compared to symptoms of PSP and domoic acid; the
DSP toxins have been reported to be tumor promoting agents. Due
to the relatively minor and generic symptoms associated with DSP,
it is difficult to diagnose whether outbreaks of this particular
poisoning have occurred in the U.S.
![](images/DSP_Toxins.gif)
Okadaic acid (OA) |
H |
H |
Me |
Dinophysistoxin-1 (DTX-1) |
H |
Me |
Me |
Dinophysistoxin-2 (DTX-2) |
H |
Me |
H |
7-O-Acyl Derivates |
CH3CO- |
|
|
{Structure after E.P. Carmody, K.J. James, S.S. Kelly, and K
Thomas in Harmful Algal Blooms (P. Lassus, G Arzul, E. Erard,
P. Gentien, C. Marcaillou Editors) 1995. page 273.}
Up until recently, DSP was managed by mouse bioassay and/or monitoring
shellfish growing waters for the presence of Dinophysis
organisms. Within the last decade or so, chromatographic tecniques
using HPLC have been developed that permit both quantification
and identification of the toxins. These techniques, while accurate,
require well equipped laboratories with expensive equipment (i.e.,
HPLCs and perhaps mass spectrometers). Recently, molecular biological
methods based on ELISA have been developed. For the future, these
procedures, offer the potential for much lower costs, rapid assessment,
and also field testing.
Because they have very large shellfish industries, Ireland and
Spain have implemented extensive monitoring programs for DSP.
Nevertheless, if monitoring costs are to be controlled, we need
a far better understanding of the life cycle of Dinophysis
and toxin production.
A full understanding of the production of the toxins by Dinophysis
has proved difficult because the laboratory culturing of this
dinoflagellate has proved very challenging. For reasons that are
not understood, the organism can only be grown through one or
two generations under laboratory conditions. Until the organism
can be cultured in sufficient quantities, studies on the toxins
will be hampered.