Award Abstract #0726229
NSF 2010 Grant: Oomycete Effector Proteins as Molecular Probes to Elucidate Arabidopsis Disease Resistance Signaling Networks

NSF Org:	IOS Division of Integrative Organismal Systems
Initial Amendment Date:	January 11, 2008
Latest Amendment Date:	January 11, 2008
Award Number:	0726229
Award Instrument:	Standard Grant
Program Manager:	Michael L. Mishkind IOS Division of Integrative Organismal Systems BIO Directorate for Biological Sciences
Start Date:	January 15, 2008
Expires:	December 31, 2010 (Estimated)
Awarded Amount to Date:	$746715
Investigator(s):	Brian Staskawicz stask@nature.berkeley.edu (Principal Investigator)
Sponsor:	University of California-Berkeley Sponsored Projects Office BERKELEY, CA 94704 510/642-8109
NSF Program(s):	THE 2010 PROJECT
Field Application(s):	0000099 Other Applications NEC
Program Reference Code(s):	BIOT, 9183, 9179, 1684
Program Element Code(s):	1684

ABSTRACT

During the evolution of plant-pathogen interactions, pathogens have acquired the capacity to deliver effector proteins into their plant hosts that re-program host defense signaling systems. This project will employ effectors produced by the oomycete plant pathogen Hyaloperonospora parasitica as molecular probes to elucidate existing and undiscovered defense signaling pathways in Arabidopsis, a major model species for studies in many areas of plant biology. The rationale is that obligate pathogens such as H. parasitica have evolved a sophisticated arsenal of virulence effector proteins that reprogram the host to allow the pathogen to proliferate. Thus, the ability to uncover the targets of these proteins in the host should reveal the function of many unknown genes in the Arabidopsis genome. Furthermore, the specific recognition of these effectors by resistance proteins and the characterization of the subsequently induced signaling pathways will allow us to assign gene function to many uncharacterized genes in these signaling networks. The elucidation of the Arabidopsis targets of the oomycete effector proteins represents a unique opportunity to gain insight into the molecular basis of obligate parasitism and to reveal novel pathways that oomycete pathogens exploit to avoid host detection and incite disease. In its broader impact, this project will potentially facilitate the development of technologies for generating crops with durable and broad-spectrum resistance. The project will also provide opportunities for undergraduates, graduate students and postdoctoral fellows to be trained not only in the biology of classical plant-pathogen interactions but to employ advanced methods of computational biology, genetics, biochemistry, structural and cell biological methods to study these processes.

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