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PubMed articles by:
Yoshii, H.
Sadaoka, K.
Matsuura, M.
Mori, Y.
Virol J. 2008; 5: 54.
Published online 2008 April 30. doi: 10.1186/1743-422X-5-54.
PMCID: PMC2412858
Copyright © 2008 Yoshii et al; licensee BioMed Central Ltd.
Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture
Hironori Yoshii,1,2 Kay Sadaoka,1 Masaaki Matsuura,1,2 Kazuhiro Nagaike,2 Michiaki Takahashi,3 Koichi Yamanishi,1 and Yasuko Moricorresponding author1
1Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, Osaka, Japan
2Kanonji Institute, the Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa, Japan
3The Research Foundation for Microbial Diseases of Osaka University, Suita, Osaka, Japan
corresponding authorCorresponding author.
Hironori Yoshii: hyoshii/at/mail.biken.or.jp; Kay Sadaoka: kaysada/at/nibio.go.jp; Masaaki Matsuura: mmatsuura/at/nibio.go.jp; Kazuhiro Nagaike: knagaike/at/mail.biken.or.jp; Michiaki Takahashi: mtakahashi/at/mail.biken.or.jp; Koichi Yamanishi: yamanishi/at/nibio.go.jp; Yasuko Mori: ymori/at/nibio.go.jp
Received January 4, 2008; Accepted April 30, 2008.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 
Abstract

Background
Open reading frame 58 (ORF58) of varicella-zoster virus (VZV) lies at the 3'end of the Unique long (UL) region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth of VZV, we constructed a deletion mutant of ORF58 (pOka-BACΔ58) from the Oka parental genome cloned into a bacterial artificial chromosome (pOka-BAC).

Results
The ORF58-deleted virus (rpOkaΔ58) was reconstituted from the pOka-BACΔ58 genome in MRC-5 cells, indicating that the ORF58 gene is non-essential for virus growth. Comparison of the growth rate of rpOkaΔ58 and recombinant wild-type virus by assessing plaque sizes revealed no significant differences between them both in MRC-5 cells and malignant melanoma cells.

Conclusion
This study shows that the ORF58 gene is dispensable for viral replication and does not affect the virus' ability to form plaques in vitro.

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