Volume 10, Number 12, December 2004

Genome Sequence and Attenuating Mutations in West Nile Virus Isolate from Mexico

David W.C. Beasley,* C. Todd Davis,* Jose Estrada-Franco,* Roberto Navarro-Lopez,† Arturo Campomanes-Cortes,† Robert B. Tesh,* Scott C. Weaver,* and Alan D.T. Barrett*
*University of Texas Medical Branch, Galveston, Texas, USA; and †Comision Mexico-Estados Unidos para la Prevencion de la Fiebre Aftosa y Otras Enfermedades Exoticas de los Animales, Mexico City, Mexico

Figure 1. Western blot showing differing mobility of E proteins from nine plaque-purified variants of West Nile virus (WNV) strain TM-171 Mex03. Nucleotide sequencing of strains in lanes 5 to 9 indicated the presence of an "NYS" glycosylation motif at residues 154 to 156 of E, while strains in lanes 1 to 4 encoded "NYP." Antigens were separated in a nonreducing 5%/10% discontinuous sodium dodecyl sulfate–polyacrylamide gel, transferred to 0.2 μm nitrocellulose and detected with WNV-specific monoclonal antibody 7H2 (6).

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This page last reviewed October 27, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention