RFE Fish Species Identification Isoelectric Focusing Tools - Yellowtail Rockfish Phast IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes flavidus
FDA Seafood List Common Name: Yellowtail Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19940913gel06
Unique Web Code: yeh01Srr
Tissue Aliquot Code: sebaflav I94-004 #07a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	68	8.3	0.0460863	6	0.432691	7.6	5.9	4.55	68.6	5.7	0.0430439
2	5.2	166	20.4	0.0638394	6	0.676429	11.8	9.2	5.2	166.7	6.3	0.0605769
3	5.85	279	34.2	0.101972	4	0.723223	12.6	9.8	5.85	279.3	4	0.102258
4	6.55	417	51.2	0.0851049	7	1.10549	19.3	15	6.55	415.9	7.4	0.0837274
5	7.35	513	62.9	0.0321563	5	0.261602	4.6	3.5	7.35	513.3	4.9	0.030365
6	8.15	638	78.3	0.0555908	9	0.786278	13.7	10.7	8.15	637.9	8.7	0.0507429
7	8.45	700	85.9	0.0620181	7	0.790825	13.8	10.7	8.45	699.7	7.3	0.0612182
8	8.65	749	91.9	0.0467473	5	0.36296	6.3	4.9	8.65	748	4.6	0.0445349
9	9.3	795	97.5	0.0873165	4	0.581678	10.2	7.9	9.3	794.2	4.4	0.0743428

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.33	39	4.8	0.0667851	9	1.0397	4.8	3.5	4.33	40.6	9	0.0655307
2	4.763	101	12.4	0.10268	9	1.5783	7.3	5.3	4.763	102.4	9.2	0.0965221
3	4.917	123	15.1	0.126055	9	0.917942	4.3	3.1	4.917	118	9.2	0.0565383
4	4.994	134	16.4	0.128738	18	3.93685	18.3	13.1	4.994	134.4	17.8	0.124771
5	5.968	303	37.2	0.413037	4	2.48936	11.6	8.3	5.968	302.2	3.6	0.391608
6	6.025	314	38.5	0.317285	7	3.68444	17.1	12.3	6.025	314.4	7.2	0.290193
7	6.169	342	42	0.0588983	3	0.281707	1.3	0.9	6.169	340.6	3.2	0.0504192
8	6.205	349	42.8	0.0570503	7	0.892013	4.1	3	6.205	351	7.4	0.0684647
9	6.236	355	43.6	0.255982	2	0.759625	3.5	2.5	6.236	354.6	2.1	0.202737
10	6.354	378	46.4	0.0619129	6	0.606669	2.8	2	6.354	377.5	6.1	0.0558538
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.468	400	49.1	0.0710167	3	0.402274	1.9	1.3	6.468	400.8	3	0.0756085
12	6.509	408	50.1	0.0940177	3	0.460376	2.1	1.5	6.509	407.7	2.8	0.0915258
13	7.433	528	64.8	0.0808077	11	1.44915	6.7	4.8	7.433	529	10.9	0.0749633
14	7.619	557	68.3	0.147817	3	0.828932	3.8	2.8	7.619	556.8	3.2	0.146137
15	8.672	749	91.9	0.0600862	22	2.20518	10.2	7.3	8.672	749	21.5	0.0578642

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	72	8.8	0.0445684	7	0.453405	8.2	6.2	4.55	72.6	6.5	0.0393132
2	5.2	160	19.7	0.0752393	5	0.687586	12.4	9.4	5.2	159.8	5.5	0.0710821
3	5.85	280	34.4	0.112821	4	0.749475	13.5	10.2	5.85	279.6	3.9	0.108578
4	6.55	414	50.9	0.0977972	7	1.18436	21.4	16.2	6.55	414.1	7.1	0.0938239
5	7.35	516	63.4	0.028763	6	0.294366	5.3	4	7.35	516.5	6	0.0279049
6	8.15	641	78.7	0.0587377	7	0.701576	12.7	9.6	8.15	640.7	7.1	0.055917
7	8.45	704	86.5	0.0632447	6	0.618724	11.2	8.4	8.45	703.6	5.7	0.0609726
8	8.65	745	91.5	0.041301	4	0.316208	5.7	4.3	8.65	744.9	4.4	0.0401056
9	9.3	789	96.9	0.0483366	7	0.538307	9.7	7.3	9.3	789.1	7.2	0.0422708

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.36	50	6.1	0.0314072	8	0.417817	2.7	2.2	4.36	51.8	7.8	0.0300793
2	4.55	83	10.2	0.0279245	4	0.190482	1.3	1	4.55	83.1	4.1	0.026119
3	4.895	143	17.6	0.178571	12	3.53798	23.3	18.2	4.895	145.5	11.6	0.172122
4	5.2	196	24.1	0.0360758	6	0.376917	2.5	1.9	5.2	194.6	6	0.0355626
5	5.85	295	36.2	0.0525357	3	0.270476	1.8	1.4	5.85	294.9	2.9	0.052529
6	5.913	306	37.6	0.456734	3	2.20018	14.5	11.3	5.913	306.6	2.7	0.455711
7	5.976	317	38.9	0.300412	6	2.67718	17.6	13.8	5.976	318.6	6.3	0.239762
8	6.147	347	42.6	0.0521709	3	0.28971	1.9	1.5	6.147	346.4	3.2	0.0511436
9	6.181	353	43.4	0.0514804	4	0.316186	2.1	1.6	6.181	353.4	3.5	0.0506182
10	6.221	360	44.2	0.297083	2	0.976928	6.4	5	6.221	359.9	1.9	0.290633
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.358	384	47.2	0.0400549	5	0.360595	2.4	1.9	6.358	384.8	5.5	0.037283
12	6.489	407	50	0.0497061	2	0.102504	0.7	0.5	6.489	407.1	1.8	0.0312918
13	6.535	415	51	0.0709406	8	0.833699	5.5	4.3	6.535	414.8	7.5	0.0623726
14	7.35	523	64.3	0.0304611	3	0.111957	0.7	0.6	7.35	522.1	3.3	0.0189612
15	7.429	535	65.7	0.0404286	12	0.753864	5	3.9	7.429	535.2	11.7	0.0362821
16	7.606	562	69	0.0814494	3	0.485888	3.2	2.5	7.606	562	3.4	0.0804274
17	8.15	645	79.2	0.0085174	4	0.0673481	0.4	0.3	8.15	642.7	4.5	0.0084889
18	8.45	706	86.7	0.0331673	6	0.332303	2.2	1.7	8.45	706.1	5.9	0.0319978
19	8.65	747	91.8	0.0452221	8	0.542123	3.6	2.8	8.65	746.7	7.9	0.0385112
20	9.3	790	97.1	0.0265105	9	0.363696	2.4	1.9	9.3	787.4	8.8	0.0233935

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.246	32	3.9	0.08769	8	1.14978	4.8	3.7	4.246	34.4	7.6	0.0856991
2	4.647	98	12	0.106509	9	1.60274	6.7	5.2	4.647	99.4	9.1	0.0994742
3	4.781	120	14.7	0.141273	8	0.590209	2.5	1.9	4.781	114.8	8	0.0416426
4	4.866	134	16.5	0.149134	20	5.19274	21.9	16.8	4.866	131	20	0.146277
5	5.884	303	37.2	0.599453	3	2.73198	11.5	8.8	5.884	303.2	2.6	0.582028
6	5.945	314	38.6	0.433903	6	4.08921	17.2	13.2	5.945	315.1	6.4	0.361234
7	6.101	342	42	0.0728658	3	0.386554	1.6	1.3	6.101	341.4	2.8	0.0769374
8	6.135	348	42.8	0.0713534	3	0.366645	1.5	1.2	6.135	348.6	2.9	0.0724464
9	6.174	355	43.6	0.414316	2	1.37096	5.8	4.4	6.174	355	1.9	0.40394
10	6.319	381	46.8	0.0644101	5	0.537452	2.3	1.7	6.319	381.2	5	0.0604062
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.403	396	48.6	0.0245783	4	0.169637	0.7	0.5	6.403	394.1	4.5	0.0213174
12	6.459	406	49.9	0.0884707	3	0.428385	1.8	1.4	6.459	406.6	2.7	0.0905952
13	6.503	414	50.9	0.111635	3	0.539251	2.3	1.7	6.503	413.8	2.8	0.108406
14	7.412	534	65.6	0.074511	12	1.60556	6.8	5.2	7.412	536.1	12	0.0754424
15	7.6	563	69.2	0.177748	3	0.912732	3.8	3	7.6	563.1	2.9	0.17568
16	8.638	748	91.9	0.0625614	19	2.07224	8.7	6.7	8.638	743.7	18.6	0.0628255

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	80	9.8	0.079476	4	0.582787	9.3	7.3	4.55	79.8	4.5	0.0732297
2	5.2	181	22.2	0.114831	4	0.73831	11.7	9.3	5.2	180.6	3.9	0.107631
3	5.85	298	36.6	0.130122	4	0.799198	12.7	10	5.85	297.8	3.6	0.124719
4	6.55	426	52.3	0.101981	6	1.0757	17.1	13.5	6.55	426.4	6.2	0.0977563
5	7.35	525	64.4	0.0297157	6	0.274192	4.4	3.4	7.35	524.9	5.6	0.0275729
6	8.15	649	79.6	0.0639454	7	0.806032	12.8	10.1	8.15	649	7.5	0.060841
7	8.45	712	87.4	0.0683197	6	0.753694	12	9.4	8.45	711.6	6.3	0.0674633
8	8.65	753	92.4	0.0564294	4	0.417719	6.6	5.2	8.65	752.1	4.4	0.0535217
9	9.3	791	97.1	0.0657765	8	0.839775	13.4	10.5	9.3	788.6	7.8	0.0607332

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 78

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.600 5.200 11.800 5.850 12.600 6.550 19.300 7.350 4.600 8.150 13.700 8.450 13.800 8.650 6.300 9.300 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.330 4.800 4.763 7.300 4.917 4.300 4.994 18.300 5.968 11.600 6.025 17.100 6.169 1.300 6.205 4.100 6.236 3.500 6.354 2.800 6.468 1.900 6.509 2.100 7.433 6.700 7.619 3.800 8.672 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 8.200 5.200 12.400 5.850 13.500 6.550 21.400 7.350 5.300 8.150 12.700 8.450 11.200 8.650 5.700 9.300 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.360 2.700 4.550 1.300 4.895 23.300 5.200 2.500 5.850 1.800 5.913 14.500 5.976 17.600 6.147 1.900 6.181 2.100 6.221 6.400 6.358 2.400 6.489 0.700 6.535 5.500 7.350 0.700 7.429 5.000 7.606 3.200 8.150 0.400 8.450 2.200 8.650 3.600 9.300 2.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
4.246 4.800 4.647 6.700 4.781 2.500 4.866 21.900 5.884 11.500 5.945 17.200 6.101 1.600 6.135 1.500 6.174 5.800 6.319 2.300 6.403 0.700 6.459 1.800 6.503 2.300 7.412 6.800 7.600 3.800 8.638 8.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 9.300 5.200 11.700 5.850 12.700 6.550 17.100 7.350 4.400 8.150 12.800 8.450 12.000 8.650 6.600 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Rockfish ---- Go to Display Gel Lane Navigator.