Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Swordfish  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Xiphias gladius
FDA Seafood List Common Name: Swordfish
FDA Seafood List Market Name: Swordfish
Unique IEF Gel Code: 19950809gel10
Unique Web Code: xgh01Srr
Tissue Aliquot Code: xiphglad I94-001 #02 Gel Analysis Conditions
Gel ImageFillet ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55738.90.044726860.42767810.77.64.5573.55.70.0419919
25.2172210.03366550.2747246.94.95.2171.84.80.0322972
35.8529235.70.033964450.280362755.85291.850.0318181
46.5542051.30.0325718160.87750421.915.66.55412.916.10.0307507
57.3552764.40.0161511100.24476.14.47.35525.810.30.0133469
68.15654800.0383465100.64636916.211.58.15652.19.70.0374983
78.4571687.50.08186760.88769522.215.88.45715.66.20.0807717
88.6575191.80.051475840.36007496.48.65751.43.90.0515783
PeakCounter: 8
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.414536.50.007026880.09357440.50.44.41454.48.20.0064697
24.6648810.80.00583950.05229110.30.24.66489.250.0058804
34.914123150.009537560.08666450.40.44.9141226.10.0079823
45.99131438.40.016174830.09639980.50.45.991313.63.40.0158179
56.06632840.10.020468740.1485640.70.76.066327.54.40.0192248
66.13134041.60.02267840.1343460.70.66.131339.53.80.0198524
76.16934742.40.01391250.1115320.60.56.169346.84.90.0129584
86.25536344.40.29230131.788598.98.36.255363.43.30.301374
96.3237545.80.057199730.3136191.61.56.32375.62.90.0602667
106.35838246.70.061831630.3294941.61.56.358382.130.0619773
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.42339448.20.073941830.3775951.91.86.423393.93.10.0681495
126.48840649.60.07572660.81049143.86.48840460.075858
136.52641350.50.23563131.181175.95.56.526413.830.222263
146.64443052.60.3704931.745858.78.16.644430.52.70.369446
156.856458560.09211630.5076422.52.46.856458.43.10.0924564
166.94647057.50.009457930.04582210.20.26.946470.22.90.0089262
177.08248859.70.10493750.9362594.64.47.082487.65.40.0969875
187.20350461.60.0892114101.534287.67.17.203503.710.50.0826521
197.30851863.30.46145532.5692212.7127.308518.53.10.460462
207.89361074.60.10652760.9599244.84.57.893611.75.80.0936956
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.09464278.50.0964244101.686788.47.88.094640.39.80.0972774
228.38469985.50.023167870.3103891.51.48.384697.37.20.0242635
238.53372788.90.02678130.151020.70.78.533725.93.40.025415
248.60173990.30.32138831.551387.77.28.601738.82.80.317984
258.68775492.20.047693680.59936232.88.687754.77.90.0427468
268.79677394.50.19501962.0520310.29.58.796772.860.192015
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55718.70.022075660.2342257.95.94.5570.35.90.0223033
25.215418.90.015600980.2077275.25.2156.380.0147367
35.8528434.80.023146850.190896.44.85.85283.74.90.0220613
46.5541550.90.0323442100.54993618.613.86.55411.8100.0309936
57.3552063.80.0081895210.2803689.57.17.35509.720.80.0076177
68.1564879.50.037985270.49869916.812.68.15647.57.50.0377594
78.45709870.069716760.68476523.117.28.457095.70.0679888
88.6574491.30.043420340.31563810.77.98.65743.84.10.0431055
PeakCounter: 8
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.396556.80.007175150.0591880.40.34.39653.750.0066847
24.55779.50.016796850.1425620.90.84.55784.90.0163384
35.217020.90.017889750.1356440.90.85.217050.0153697
45.8528935.60.01538840.1143740.70.75.85288.84.10.0156086
56.10433541.30.008414840.05221030.30.36.104334.23.80.0078294
66.1734742.70.00885140.05663130.40.36.17346.13.80.0085127
76.225357440.007569760.05653160.40.36.225356.56.20.0051594
86.29637045.60.25669831.283538.17.46.296370.62.70.264664
96.35738146.90.034478620.15782510.96.357381.42.50.0359596
106.3938747.70.032543730.1702451.116.39387.430.0324915
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.45639949.10.040209630.2053861.31.26.456398.53.30.0356009
126.50640850.20.051300260.5085853.22.96.506407.15.60.0515634
136.5541651.20.14697441.014076.45.96.55416.440.141667
146.67343253.20.20789931.19897.66.96.673431.83.20.209611
156.87445856.40.061483740.4227262.72.46.874457.83.90.0607859
167.08248559.70.074587160.7059674.54.17.082485.45.60.0711627
177.19850061.60.062133180.8438525.34.97.198498.77.80.0612322
187.30651463.30.37858542.4519915.514.27.306513.63.60.380528
197.94161575.70.048619690.6152323.93.67.941614.58.70.0399597
208.10964279.10.086811191.323948.47.78.109641.88.90.0843773
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.33468784.60.019590870.1973761.21.18.334688.26.50.0171266
228.4571187.60.037767650.30946221.88.45711.350.034576
238.571731900.22295131.092056.96.38.571730.62.70.224366
248.6574491.60.0467481100.8412585.34.98.65748.610.20.0466584
258.79576894.60.1661871.8784811.910.98.795767.76.70.15845
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.392475.80.008037180.1077850.60.54.39245.88.40.0072631
24.614809.90.005837150.04376480.20.24.61481.14.60.0053763
34.84311414.10.006726460.06693130.30.34.843112.96.10.0061913
45.93830337.50.010999140.07415750.40.45.938303.53.70.0113224
56.03732139.70.018357850.1358770.70.66.037319.74.50.0169793
66.11533541.40.012325740.08818740.50.46.115335.84.20.0117866
76.1734542.60.011520240.08397690.40.46.17345.24.20.0113028
86.26936344.90.30488831.508387.77.16.269362.92.80.301262
96.33537546.40.060960630.3443981.81.66.335374.53.10.0633685
106.36838147.10.060598730.3630011.91.76.368381.23.30.0614477
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.4439448.70.070544730.3761491.91.86.44393.53.20.0672583
126.48440249.70.063905660.6979463.63.36.484402.85.60.070203
136.53941250.90.2202331.262386.466.539412.83.40.208999
146.65942852.90.32748731.650118.47.86.659427.92.80.331894
156.87145556.20.078368630.4432952.32.16.871454.43.10.0800983
167.07448159.50.11038750.7274123.73.47.074480.74.80.0863816
177.18349561.20.0886897121.778549.18.47.18349411.80.0847257
187.29350962.90.45748632.5637613.112.17.293509.23.30.439408
197.93361175.50.061831270.7384693.83.57.933611.27.40.0564045
208.11164079.10.11050991.7647298.48.111640.29.50.105004
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.394696860.025600180.3620311.81.78.394692.97.70.0264082
228.525720890.021471930.09443070.50.48.525719.42.60.0203393
238.5973190.40.29967231.41187.26.78.59731.22.70.295942
248.69874992.60.058407790.9552384.94.58.698748.39.10.0592448
258.78776494.40.1982461.930269.99.18.787763.85.70.192039
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55718.80.042774250.37863911.47.94.5571.25.30.0403199
25.217121.10.031335150.2571167.85.45.2170.850.0290676
35.8529236.10.029846750.2501747.65.35.852924.90.0290208
46.5541951.80.0364612100.6298211913.26.55416.510.40.0340529
57.3552064.30.012470180.1725015.23.67.35520.48.20.0118594
68.1565180.50.034402890.51768515.710.98.15650.49.10.0320468
78.4571187.90.075007260.7714523.316.28.45710.75.90.0740282
88.6574592.10.049243740.330454106.98.65744.53.90.0479448
PeakCounter: 8
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 100
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 8 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 8 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 8 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln