RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Swordfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Xiphias gladius
FDA Seafood List Common Name: Swordfish
FDA Seafood List Market Name: Swordfish
Unique IEF Gel Code: 19950809gel16
Unique Web Code: xgg01Srr
Tissue Aliquot Code: xiphglad I94-001 #02 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	30	2.7	0.126458	10	2.21257	12.8	9.9	3.5	33	10.5	0.119431
2	4.55	187	16.5	0.166966	5	1.39132	8.1	6.2	4.55	186.4	4.9	0.159271
3	5.2	275	24.3	0.179792	5	1.38823	8.1	6.2	5.2	274.5	4.5	0.172703
4	5.85	424	37.5	0.261745	4	1.69443	9.8	7.6	5.85	424	3.6	0.262716
5	6.55	593	52.4	0.146333	6	1.32978	7.7	5.9	6.55	590.8	5.7	0.131424
6	6.85	634	56	0.0773835	5	0.672841	3.9	3	6.85	634.2	5.4	0.0707041
7	7.35	726	64.1	0.140577	6	1.47634	8.6	6.6	7.35	724.3	6.2	0.135006
8	8.15	886	78.3	0.0644516	9	0.981412	5.7	4.4	8.15	886.7	8.8	0.0629061
9	8.45	951	84	0.112566	6	1.13216	6.6	5	8.45	951.1	5.8	0.11066
10	8.65	992	87.6	0.0958439	6	0.93433	5.4	4.2	8.65	992.2	5.6	0.0945304
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1111	98.1	0.409326	6	4.0134	23.3	17.9	9.3	1109.4	6.3	0.362161

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.661	210	18.5	0.0400116	9	0.540672	2.2	1.9	4.661	208.6	8.6	0.0356495
2	5.004	258	22.7	0.0196466	6	0.19503	0.8	0.7	5.004	254.5	5.7	0.0193613
3	5.22	290	25.6	0.0256392	15	0.696428	2.8	2.4	5.22	288.7	14.7	0.0266565
4	5.311	311	27.4	0.0171746	9	0.272327	1.1	1	5.311	321.9	9.4	0.0162959
5	5.63	384	33.8	0.0207111	9	0.258714	1.1	0.9	5.63	383.8	8.5	0.0171527
6	5.903	447	39.4	0.0260636	8	0.29402	1.2	1	5.903	448.2	7.9	0.0210618
7	6.017	474	41.8	0.0172893	6	0.164174	0.7	0.6	6.017	473.4	5.6	0.016657
8	6.089	491	43.3	0.0498184	6	0.443272	1.8	1.5	6.089	490.8	5.5	0.0451211
9	6.22	522	46	0.318988	3	1.75292	7.2	6.1	6.22	521.7	3	0.324272
10	6.284	537	47.3	0.0836171	3	0.427178	1.7	1.5	6.284	537	3.3	0.0733738
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.317	545	48	0.117879	5	0.974733	4	3.4	6.317	543.9	5.4	0.102492
12	6.398	564	49.7	0.0576011	3	0.265612	1.1	0.9	6.398	564.3	2.8	0.0532023
13	6.436	573	50.5	0.0304249	4	0.189366	0.8	0.7	6.436	572.1	4	0.0269727
14	6.457	578	50.9	0.0262649	3	0.166633	0.7	0.6	6.457	579.2	3.4	0.0273999
15	6.525	594	52.3	0.0500732	3	0.272813	1.1	1	6.525	594.6	3.3	0.0472907
16	6.565	602	53	0.188946	2	0.612915	2.5	2.1	6.565	602.1	1.9	0.185438
17	6.85	641	56.5	0.0747196	3	0.390372	1.6	1.4	6.85	640.7	3.1	0.0715679
18	7.073	682	60.1	0.0288453	3	0.181097	0.7	0.6	7.073	681.1	3.5	0.029435
19	7.165	699	61.6	0.0254691	9	0.418033	1.7	1.5	7.165	696.4	8.9	0.0264657
20	7.345	732	64.5	0.338808	3	2.11244	8.6	7.4	7.345	732	3.5	0.341165
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.125	887	78.1	0.0812877	13	1.84238	7.5	6.4	8.125	884.3	13.5	0.0770779
22	8.322	929	81.9	0.0203804	9	0.311742	1.3	1.1	8.322	929.3	8.8	0.0200705
23	8.482	963	84.8	0.0823308	6	0.827822	3.4	2.9	8.482	962.2	6.3	0.0744664
24	8.555	978	86.2	0.251549	5	2.0532	8.4	7.2	8.555	978.4	4.6	0.253783
25	8.876	1038	91.5	0.234393	22	8.85029	36.1	30.9	8.876	1038.1	21.7	0.230593

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	3.4	0.131851	16	3.51427	18.6	15	3.5	40.1	16.1	0.123162
2	4.55	194	17.2	0.211773	4	1.43397	7.6	6.1	4.55	193.7	4.2	0.193001
3	5.2	288	25.5	0.213622	4	1.32453	7	5.7	5.2	287.8	3.7	0.203599
4	5.85	437	38.6	0.252077	4	1.63776	8.7	7	5.85	436.6	3.6	0.258072
5	6.55	599	53	0.145076	6	1.30949	6.9	5.6	6.55	597.4	5.7	0.130629
6	6.85	640	56.6	0.0833315	6	0.751129	4	3.2	6.85	640.6	5.7	0.0744693
7	7.35	732	64.7	0.138774	6	1.48533	7.9	6.4	7.35	729.7	6.5	0.129092
8	8.15	890	78.7	0.0727313	9	1.07029	5.7	4.6	8.15	889.7	8.7	0.0695305
9	8.45	954	84.4	0.135899	6	1.40759	7.4	6	8.45	953.1	5.9	0.134311
10	8.65	995	88	0.116581	6	1.1803	6.2	5	8.65	994	5.8	0.113982
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1109	98.1	0.333892	8	3.79603	20.1	16.2	9.3	1107.5	7.6	0.283574

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	46	4.1	0.103574	17	2.99129	7.8	7.3	3.5	50	17.4	0.0969585
2	4.55	209	18.5	0.153725	4	0.956106	2.5	2.3	4.55	208.6	3.9	0.139294
3	4.633	221	19.6	0.0478936	7	0.755121	2	1.8	4.633	219	7.5	0.0571578
4	4.799	245	21.7	0.02293	6	0.24524	0.6	0.6	4.799	243.4	6	0.0230946
5	4.951	267	23.6	0.0623244	3	0.298644	0.8	0.7	4.951	267	2.8	0.0591601
6	5.117	291	25.8	0.0212613	6	0.214477	0.6	0.5	5.117	290.9	5.9	0.020595
7	5.2	303	26.8	0.132422	4	0.966186	2.5	2.3	5.2	302.7	4.2	0.129396
8	5.285	322	28.5	0.03229	8	0.478371	1.3	1.2	5.285	324.7	7.8	0.0347276
9	5.469	363	32.1	0.0193317	4	0.159351	0.4	0.4	5.469	361.6	4.1	0.0221559
10	5.545	380	33.6	0.0181108	7	0.223258	0.6	0.5	5.545	378.3	6.5	0.0192915
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.599	392	34.7	0.0280709	4	0.184061	0.5	0.4	5.599	391	3.5	0.0296706
12	5.698	414	36.6	0.0218666	5	0.157974	0.4	0.4	5.698	413.3	4.8	0.0186885
13	5.85	448	39.6	0.210837	3	1.23831	3.2	3	5.85	448.2	3.4	0.208088
14	5.991	479	42.4	0.0135364	5	0.145108	0.4	0.4	5.991	480	5	0.016355
15	6.077	498	44.1	0.0373572	8	0.459334	1.2	1.1	6.077	500.5	7.9	0.0327107
16	6.218	529	46.8	0.311971	3	1.76734	4.6	4.3	6.218	529.5	3.1	0.321948
17	6.3	547	48.4	0.0988707	3	0.535462	1.4	1.3	6.3	545.6	3.1	0.0968098
18	6.327	553	48.9	0.0770338	4	0.543096	1.4	1.3	6.327	552.9	3.9	0.0794183
19	6.35	558	49.4	0.039403	3	0.176422	0.5	0.4	6.35	558.9	3	0.0328123
20	6.414	572	50.6	0.0535937	3	0.228221	0.6	0.6	6.414	572.3	2.7	0.0476064
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.482	587	51.9	0.0267989	10	0.418227	1.1	1	6.482	587.3	10	0.0236142
22	6.55	602	53.3	0.0970621	5	0.800441	2.1	1.9	6.55	602.6	4.7	0.0959141
23	6.613	610	54	0.213755	1	0.541465	1.4	1.3	6.613	609.9	1.5	0.205429
24	6.85	640	56.6	0.0354886	5	0.383591	1	0.9	6.85	642.1	4.6	0.0471117
25	6.893	648	57.3	0.0738496	2	0.279409	0.7	0.7	6.893	648.4	2.4	0.0644884
26	7.1	686	60.7	0.0298159	4	0.177143	0.5	0.4	7.1	686.8	3.6	0.0279508
27	7.154	696	61.6	0.0303113	4	0.194875	0.5	0.5	7.154	696	3.6	0.0307693
28	7.203	705	62.4	0.0200429	6	0.280294	0.7	0.7	7.203	706.7	6.4	0.0246971
29	7.35	732	64.8	0.116179	6	1.17353	3.1	2.8	7.35	732.3	6.3	0.105863
30	7.385	739	65.4	0.332895	3	1.46122	3.8	3.5	7.385	738.9	2.7	0.305455
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	890	78.8	0.130049	12	2.65765	7	6.4	8.15	889.6	12.2	0.122738
32	8.344	932	82.5	0.0188454	7	0.244925	0.6	0.6	8.344	929	6.8	0.0203338
33	8.45	955	84.5	0.105838	10	1.95071	5.1	4.7	8.45	959.8	9.8	0.112372
34	8.564	979	86.6	0.242101	5	2.21775	5.8	5.4	8.564	979	4.9	0.253117
35	8.65	997	88.2	0.0800376	6	0.615414	1.6	1.5	8.65	997.8	6.5	0.0537836
36	8.844	1031	91.2	0.216926	24	8.81277	23	21.4	8.844	1032.6	24	0.207174
37	9.3	1111	98.3	0.377374	5	3.30366	8.6	8	9.3	1109.4	5.5	0.340561

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.652	211	18.7	0.0363348	9	0.491906	1.9	1.5	4.652	211.5	9.5	0.029325
2	5	259	22.9	0.0211758	4	0.15093	0.6	0.5	5	257.8	4.2	0.0204352
3	5.227	293	25.9	0.0241813	14	0.606374	2.3	1.9	5.227	290.6	13.5	0.0253083
4	5.32	315	27.9	0.0270679	8	0.269174	1	0.8	5.32	317.6	8.1	0.0188278
5	5.471	351	31.1	0.0141264	7	0.131649	0.5	0.4	5.471	352.8	6.5	0.0113618
6	5.602	382	33.8	0.0217783	5	0.146675	0.6	0.5	5.602	381.3	4.7	0.0175158
7	5.876	447	39.6	0.0347169	5	0.316292	1.2	1	5.876	446.9	5.3	0.0339812
8	6.058	488	43.2	0.0213566	7	0.25594	1	0.8	6.058	490.8	6.5	0.0220838
9	6.208	522	46.2	0.372254	3	1.72172	6.5	5.4	6.208	522.4	2.6	0.373097
10	6.287	540	47.8	0.0994818	4	0.703384	2.6	2.2	6.287	539.1	4.2	0.0947141
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.336	551	48.8	0.0387294	6	0.39244	1.5	1.2	6.336	549.1	6	0.0368304
12	6.389	563	49.8	0.0351718	2	0.130215	0.5	0.4	6.389	563.9	1.9	0.0394363
13	6.455	578	51.2	0.0149126	6	0.18617	0.7	0.6	6.455	576.9	5.7	0.0183046
14	6.526	594	52.6	0.0247401	5	0.204845	0.8	0.6	6.526	594.8	4.6	0.0250139
15	6.569	602	53.3	0.191292	2	0.662077	2.5	2.1	6.569	601.7	2	0.190962
16	6.858	640	56.6	0.066462	3	0.328038	1.2	1	6.858	639.3	2.9	0.0638146
17	7.118	688	60.9	0.0440017	5	0.369435	1.4	1.2	7.118	688.2	4.8	0.0437951
18	7.199	703	62.2	0.0244286	6	0.261087	1	0.8	7.199	701.4	6	0.0243506
19	7.37	735	65	0.399125	3	1.97038	7.4	6.2	7.37	734.4	2.7	0.40743
20	8.11	883	78.1	0.0838702	13	1.85938	7	5.8	8.11	882.8	12.8	0.0817575
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.29	920	81.4	0.0288688	9	0.467869	1.8	1.5	8.29	922.7	8.6	0.0306635
22	8.491	961	85	0.166372	9	2.3749	8.9	7.4	8.491	959.8	8.6	0.15549
23	8.551	973	86.1	0.229361	4	1.47177	5.5	4.6	8.551	973	4.2	0.19865
24	8.848	1028	91	0.278956	24	11.0729	41.7	34.6	8.848	1031.7	24	0.260305

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	57	5.1	0.11554	23	4.6034	23.3	18.7	3.5	53.7	22.6	0.115147
2	4.55	184	16.3	0.204759	4	1.33487	6.8	5.4	4.55	184.1	4	0.190046
3	5.2	269	23.8	0.197543	4	1.34645	6.8	5.5	5.2	268.7	3.9	0.192809
4	5.85	433	38.4	0.245268	4	1.65963	8.4	6.8	5.85	432.8	3.7	0.255383
5	6.55	596	52.8	0.14265	6	1.25157	6.3	5.1	6.55	594.3	5.6	0.125457
6	6.85	636	56.4	0.109441	5	0.9416	4.8	3.8	6.85	636.1	5.3	0.100048
7	7.35	729	64.6	0.155142	6	1.57492	8	6.4	7.35	727.8	6.4	0.139537
8	8.15	891	79	0.0618425	9	0.942519	4.8	3.8	8.15	891.3	8.9	0.059584
9	8.45	950	84.2	0.135121	6	1.4922	7.6	6.1	8.45	949.9	6.4	0.130816
10	8.65	987	87.5	0.107959	6	1.13389	5.7	4.6	8.65	987.1	6	0.106157
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1106	98	0.283235	8	3.47939	17.6	14.2	9.3	1103.3	7.9	0.247471

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 119

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 12.800 4.550 8.100 5.200 8.100 5.850 9.800 6.550 7.700 6.850 3.900 7.350 8.600 8.150 5.700 8.450 6.600 8.650 5.400 9.300 23.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 11 Return to Lane Navigator
4.661 2.200 5.004 0.800 5.220 2.800 5.311 1.100 5.630 1.100 5.903 1.200 6.017 0.700 6.089 1.800 6.220 7.200 6.284 1.700 6.317 4.000 6.398 1.100 6.436 0.800 6.457 0.700 6.525 1.100 6.565 2.500 6.850 1.600 7.073 0.700 7.165 1.700 7.345 8.600 8.125 7.500 8.322 1.300 8.482 3.400 8.555 8.400 8.876 36.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 9 PeakCount: 25 Return to Lane Navigator
3.500 18.600 4.550 7.600 5.200 7.000 5.850 8.700 6.550 6.900 6.850 4.000 7.350 7.900 8.150 5.700 8.450 7.400 8.650 6.200 9.300 20.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 7.800 4.550 2.500 4.633 2.000 4.799 0.600 4.951 0.800 5.117 0.600 5.200 2.500 5.285 1.300 5.469 0.400 5.545 0.600 5.599 0.500 5.698 0.400 5.850 3.200 5.991 0.400 6.077 1.200 6.218 4.600 6.300 1.400 6.327 1.400 6.350 0.500 6.414 0.600 6.482 1.100 6.550 2.100 6.613 1.400 6.850 1.000 6.893 0.700 7.100 0.500 7.154 0.500 7.203 0.700 7.350 3.100 7.385 3.800 8.150 7.000 8.344 0.600 8.450 5.100 8.564 5.800 8.650 1.600 8.844 23.000 9.300 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 37 Return to Lane Navigator
4.652 1.900 5.000 0.600 5.227 2.300 5.320 1.000 5.471 0.500 5.602 0.600 5.876 1.200 6.058 1.000 6.208 6.500 6.287 2.600 6.336 1.500 6.389 0.500 6.455 0.700 6.526 0.800 6.569 2.500 6.858 1.200 7.118 1.400 7.199 1.000 7.370 7.400 8.110 7.000 8.290 1.800 8.491 8.900 8.551 5.500 8.848 41.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 24 Return to Lane Navigator
3.500 23.300 4.550 6.800 5.200 6.800 5.850 8.400 6.550 6.300 6.850 4.800 7.350 8.000 8.150 4.800 8.450 7.600 8.650 5.700 9.300 17.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Swordfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Swordfish ---- Go to Display Gel Lane Navigator.