RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Theragra chalcogramma
FDA Seafood List Common Name: Walleye Pollock
FDA Seafood List Market Name: Pollock (Alaska Pollock)
Unique IEF Gel Code: 19970130gel10
Unique Web Code: wyh03Srr
Tissue Aliquot Code: therchal F96-004 #06m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	34	4.4	0.0934094	6	0.91192	8.8	6.5	4.55	34.9	6.1	0.0842751
2	5.2	123	16	0.124292	5	0.956778	9.3	6.8	5.2	123.5	4.5	0.11869
3	5.85	213	27.6	0.176386	3	1.00673	9.7	7.2	5.85	213.1	3.3	0.173155
4	6.55	349	45.3	0.122273	8	1.66748	16.1	11.9	6.55	346.5	8.1	0.116803
5	6.85	384	49.8	0.073228	4	0.52227	5.1	3.7	6.85	384.1	4.1	0.0710724
6	7.35	454	58.9	0.0997233	4	0.730639	7.1	5.2	7.35	453.9	4.3	0.0969209
7	8.15	587	76.1	0.138919	7	1.70963	16.5	12.2	8.15	587	7.1	0.135878
8	8.45	655	85	0.153161	7	1.82533	17.7	13.1	8.45	653.4	6.8	0.150936
9	8.65	706	91.6	0.128983	4	0.868063	8.4	6.2	8.65	705.5	4	0.122638
10	9.3	760	98.6	0.0304175	2	0.140463	1.4	1	9.3	759.9	2.5	0.031908

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.457	20	2.6	0.121127	4	0.455478	1.8	1.4	4.457	19.9	3.6	0.0716372
2	4.621	42	5.5	0.153625	24	6.3696	24.5	19.5	4.621	45.7	23.9	0.150361
3	4.97	89	11.6	0.0171739	1	0	0	0	4.97	85	1	0
4	5.17	116	15.1	0.0227253	6	0.186864	0.7	0.6	5.17	116.8	5.5	0.0190263
5	5.262	129	16.8	0.0239279	4	0.149725	0.6	0.5	5.262	128.9	4.2	0.0199348
6	5.364	144	18.7	0.450817	3	2.76385	10.6	8.5	5.364	144.3	3.4	0.45525
7	5.426	153	19.9	0.327748	3	1.90911	7.4	5.8	5.426	153	3.4	0.318247
8	5.556	172	22.3	0.0272964	5	0.19063	0.7	0.6	5.556	171.3	5.2	0.0205861
9	5.672	189	24.5	0.0255851	8	0.306602	1.2	0.9	5.672	189.6	7.8	0.022297
10	5.836	213	27.7	0.353425	5	3.04882	11.7	9.3	5.836	213.2	4.8	0.361152
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.928	230	29.9	0.191315	7	2.12633	8.2	6.5	5.928	230.3	6.6	0.182644
12	6.016	247	32.1	0.0228022	8	0.316206	1.2	1	6.016	244.6	7.5	0.0236863
13	6.094	262	34	0.129173	4	0.906933	3.5	2.8	6.094	261.2	4	0.128265
14	6.12	267	34.7	0.094325	3	0.474223	1.8	1.5	6.12	267.2	3.5	0.0774118
15	6.166	276	35.8	0.1704	3	0.995475	3.8	3.1	6.166	276.9	3.3	0.168865
16	6.265	295	38.3	0.100264	2	0.437089	1.7	1.3	6.265	295.5	2.4	0.104341
17	6.431	327	42.5	0.196487	8	2.36273	9.1	7.2	6.431	327.3	7.6	0.175624
18	7.339	454	59	0.0545127	10	0.899345	3.5	2.8	7.339	454	10.2	0.0498361
19	7.545	487	63.2	0.0723343	18	2.05824	7.9	6.3	7.545	488.7	17.6	0.0658134

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	32	4.2	0.0975406	4	0.614933	7	4.9	4.55	31.7	3.6	0.097479
2	5.2	118	15.3	0.0944282	6	0.940236	10.6	7.6	5.2	117.5	5.5	0.0958116
3	5.85	218	28.3	0.142727	4	0.921622	10.4	7.4	5.85	217.7	3.7	0.139977
4	6.55	352	45.7	0.118424	9	1.68012	19	13.5	6.55	350.4	8.7	0.109234
5	6.85	387	50.3	0.0745652	5	0.580758	6.6	4.7	6.85	387	4.7	0.0696548
6	7.35	458	59.5	0.0758285	5	0.60412	6.8	4.9	7.35	458.1	4.6	0.0736923
7	8.15	584	75.8	0.119359	6	1.31531	14.9	10.6	8.15	583.9	6.3	0.117164
8	8.45	650	84.4	0.114426	7	1.3483	15.2	10.8	8.45	648.8	6.9	0.109971
9	8.65	702	91.2	0.0974401	4	0.694829	7.9	5.6	8.65	702.1	4.1	0.0966186
10	9.3	760	98.7	0.0210142	4	0.144354	1.6	1.2	9.3	759.2	4.1	0.0200189

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.418	22	2.9	0.0131547	2	0.0318879	0.1	0.1	4.418	20.7	2.1	0.008369
2	4.55	37	4.8	0.0732091	8	0.626699	2.4	2.1	4.55	41.2	8	0.0442775
3	4.62	45	5.8	0.100366	19	2.98968	11.4	9.8	4.62	55.9	19.4	0.0871536
4	5.2	111	14.4	0.0441617	4	0.268808	1	0.9	5.2	111.2	4.5	0.0340363
5	5.359	138	17.9	0.16533	11	3.44802	13.2	11.3	5.359	138	11.2	0.173089
6	5.863	224	29.1	0.214664	7	2.2806	8.7	7.5	5.863	220.8	7.3	0.17575
7	5.966	243	31.6	0.149956	19	4.43837	17	14.6	5.966	242.5	18.7	0.133588
8	6.141	275	35.7	0.184446	7	2.11011	8.1	6.9	6.141	275.4	7.3	0.162005
9	6.217	289	37.5	0.170493	3	0.67506	2.6	2.2	6.217	289.5	2.8	0.135696
10	6.315	307	39.9	0.0799856	2	0.344711	1.3	1.1	6.315	307.2	2.4	0.0800917
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.457	333	43.2	0.174388	8	2.22141	8.5	7.3	6.457	334	7.5	0.167051
12	6.55	350	45.5	0.0304633	5	0.272547	1	0.9	6.55	348.4	4.8	0.0318717
13	6.85	387	50.3	0.0381941	7	0.451987	1.7	1.5	6.85	386.8	7.3	0.0350352
14	7.35	462	60	0.0852464	6	0.790735	3	2.6	7.35	461.9	5.7	0.0778088
15	7.456	479	62.2	0.0585266	6	0.517407	2	1.7	7.456	477.7	5.6	0.0518789
16	7.575	498	64.7	0.0565149	13	1.22084	4.7	4	7.575	500.7	12.8	0.0537491
17	8.15	590	76.6	0.21535	7	1.92352	7.4	6.3	8.15	587.3	6.5	0.166307
18	8.45	653	84.8	0.0763003	7	0.992019	3.8	3.3	8.45	652.7	7.3	0.0761775
19	8.65	705	91.6	0.0602928	4	0.433537	1.7	1.4	8.65	704.9	4.2	0.0587814
20	9.3	759	98.6	0.0142459	4	0.103244	0.4	0.3	9.3	759.7	3.9	0.0148402

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.338	15	1.9	0.0077655	3	0.0456561	0.2	0.1	4.338	15.1	3.3	0.0076988
2	4.496	34	4.4	0.136299	5	0.373511	1.3	1	4.496	35.4	5.4	0.0386896
3	4.621	49	6.4	0.203995	18	6.35572	22.1	17.3	4.621	53.8	18	0.199217
4	4.987	93	12.1	0.0385301	18	0.780392	2.7	2.1	4.987	95.6	18	0.024461
5	5.171	115	14.9	0.0104428	1	0	0	0	5.171	109	1	0
6	5.257	128	16.6	0.02365	3	0.0893183	0.3	0.2	5.257	128.2	2.8	0.0179311
7	5.34	142	18.4	0.482307	3	2.45717	8.5	6.7	5.34	142.3	2.9	0.474473
8	5.406	153	19.8	0.333414	3	1.8515	6.4	5.1	5.406	152.8	3.3	0.319692
9	5.52	172	22.3	0.0175195	3	0.0871665	0.3	0.2	5.52	172.9	2.8	0.0177984
10	5.567	180	23.3	0.0346216	2	0.145568	0.5	0.4	5.567	179.9	2.4	0.0344583
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.699	202	26.2	0.028713	8	0.38196	1.3	1	5.699	200.3	8.3	0.0260831
12	5.758	212	27.5	0.0190017	3	0.0647891	0.2	0.2	5.758	212.7	2.5	0.0145978
13	5.848	227	29.4	0.429122	4	3.11377	10.8	8.5	5.848	227.8	4.2	0.419435
14	5.94	244	31.6	0.253597	10	3.60792	12.5	9.8	5.94	244	9.9	0.204727
15	6.08	270	35	0.20944	6	2.0046	7	5.5	6.08	271.7	5.9	0.19261
16	6.167	286	37.1	0.212292	2	0.722686	2.5	2	6.167	286.2	2.1	0.193691
17	6.269	305	39.6	0.149088	2	0.527929	1.8	1.4	6.269	305.5	1.9	0.160635
18	6.426	334	43.3	0.222156	6	2.23295	7.8	6.1	6.426	334.8	6.4	0.197218
19	7.346	462	59.9	0.0694617	14	1.54607	5.4	4.2	7.346	461.2	14.2	0.0614064
20	7.568	498	64.6	0.103955	15	2.4187	8.4	6.6	7.568	498.3	15.2	0.089868

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	47	6.1	0.0737216	6	0.754874	7.1	5.2	4.55	47.1	5.7	0.0752878
2	5.2	129	16.6	0.110423	5	0.983862	9.2	6.8	5.2	128.4	5.1	0.10991
3	5.85	236	30.5	0.163669	4	1.18776	11.1	8.2	5.85	235.5	4.2	0.159011
4	6.55	367	47.4	0.146872	9	2.10761	19.7	14.6	6.55	365.1	9	0.131947
5	6.85	399	51.5	0.0919808	4	0.641751	6	4.4	6.85	399.1	4.1	0.0873557
6	7.35	466	60.1	0.131961	4	0.958842	9	6.6	7.35	465.4	4.2	0.129681
7	8.15	599	77.3	0.135237	6	1.47008	13.8	10.2	8.15	598.6	6.2	0.133876
8	8.45	659	85	0.146948	7	1.68511	15.8	11.7	8.45	658.5	6.5	0.145434
9	8.65	714	92.1	0.11315	4	0.840606	7.9	5.8	8.65	713.5	4.3	0.110597
10	9.3	770	99.4	0.0089218	3	0.050495	0.5	0.3	9.3	770.7	3.1	0.0090544

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 89

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.800 5.200 9.300 5.850 9.700 6.550 16.100 6.850 5.100 7.350 7.100 8.150 16.500 8.450 17.700 8.650 8.400 9.300 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.457 1.800 4.621 24.500 4.970 0 5.170 0.700 5.262 0.600 5.364 10.600 5.426 7.400 5.556 0.700 5.672 1.200 5.836 11.700 5.928 8.200 6.016 1.200 6.094 3.500 6.120 1.800 6.166 3.800 6.265 1.700 6.431 9.100 7.339 3.500 7.545 7.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 19 Return to Lane Navigator
4.550 7.000 5.200 10.600 5.850 10.400 6.550 19.000 6.850 6.600 7.350 6.800 8.150 14.900 8.450 15.200 8.650 7.900 9.300 1.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.418 0.100 4.550 2.400 4.620 11.400 5.200 1.000 5.359 13.200 5.863 8.700 5.966 17.000 6.141 8.100 6.217 2.600 6.315 1.300 6.457 8.500 6.550 1.000 6.850 1.700 7.350 3.000 7.456 2.000 7.575 4.700 8.150 7.400 8.450 3.800 8.650 1.700 9.300 0.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
4.338 0.200 4.496 1.300 4.621 22.100 4.987 2.700 5.171 0 5.257 0.300 5.340 8.500 5.406 6.400 5.520 0.300 5.567 0.500 5.699 1.300 5.758 0.200 5.848 10.800 5.940 12.500 6.080 7.000 6.167 2.500 6.269 1.800 6.426 7.800 7.346 5.400 7.568 8.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 7.100 5.200 9.200 5.850 11.100 6.550 19.700 6.850 6.000 7.350 9.000 8.150 13.800 8.450 15.800 8.650 7.900 9.300 0.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Walleye Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.