RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Chub Mackerel ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Scomber japonicus
FDA Seafood List Common Name: Chub Mackerel
FDA Seafood List Market Name: Mackerel, Chub
Unique IEF Gel Code: 19970219gel02
Unique Web Code: sjh01Srr
Tissue Aliquot Code: scomjapo I96-003 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.074258	4	0.464549	4.4	3	4.55	19.5	3.9	0.0679178
2	5.2	111	14.3	0.096382	5	0.770596	7.3	4.9	5.2	110.8	4.8	0.0896609
3	5.85	219	28.3	0.0605622	3	0.337064	3.2	2.1	5.85	218.6	3.2	0.0590913
4	6.55	355	45.9	0.189375	6	1.7547	16.6	11.2	6.55	354.2	5.6	0.178093
5	6.85	387	50	0.0772942	6	0.696879	6.6	4.4	6.85	386.6	5.9	0.0668314
6	7.35	452	58.4	0.156956	3	0.942258	8.9	6	7.35	451.8	3.4	0.155343
7	8.15	582	75.2	0.166974	7	2.01458	19	12.8	8.15	581.7	6.8	0.167454
8	8.45	647	83.6	0.210986	7	2.44632	23.1	15.6	8.45	646.2	6.7	0.206259
9	8.65	702	90.7	0.155571	4	1.07003	10.1	6.8	8.65	701	4	0.152774
10	9.3	758	97.9	0.0156989	3	0.0790546	0.7	0.5	9.3	757.8	2.6	0.0172532

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.114	100	12.9	0.0557932	4	0.364218	1.3	1	5.114	99.7	3.6	0.0563203
2	5.197	112	14.5	0.101766	5	0.812727	2.8	2.3	5.197	111.9	4.7	0.0979768
3	5.487	158	20.4	0.166206	3	0.851034	3	2.4	5.487	157.4	2.8	0.171021
4	5.557	169	21.8	0.10586	2	0.355154	1.2	1	5.557	168.6	1.8	0.11194
5	5.746	199	25.7	0.0347144	2	0.111659	0.4	0.3	5.746	198.8	1.9	0.033282
6	5.784	205	26.5	0.0471842	3	0.255609	0.9	0.7	5.784	204.5	3.1	0.0469168
7	5.908	227	29.3	0.0959353	4	0.603664	2.1	1.7	5.908	227	3.5	0.0962175
8	5.949	235	30.4	0.0241849	4	0.138758	0.5	0.4	5.949	234.9	3.7	0.0210588
9	6.045	254	32.8	0.0143815	4	0.0844271	0.3	0.2	6.045	253.6	3.7	0.012799
10	6.213	287	37.1	0.0478151	4	0.279836	1	0.8	6.213	285.8	3.9	0.0405136
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.345	313	40.4	0.0647533	3	0.287412	1	0.8	6.345	312.9	2.5	0.0646064
12	6.421	328	42.4	0.459831	2	1.99992	7	5.6	6.421	328.5	2.4	0.468495
13	6.482	340	43.9	0.0826898	4	0.573824	2	1.6	6.482	339.5	3.8	0.0856186
14	6.522	348	45	0.24924	3	1.13316	4	3.2	6.522	348.2	2.7	0.232738
15	6.601	359	46.4	0.451117	2	1.44153	5	4	6.601	358.6	1.8	0.445512
16	6.748	375	48.4	0.0713051	8	0.828564	2.9	2.3	6.748	378	7.6	0.0611198
17	7.12	420	54.3	0.140183	4	1.00057	3.5	2.8	7.12	420.8	4.2	0.135395
18	7.191	429	55.4	0.156041	3	0.945009	3.3	2.6	7.191	429.1	3.3	0.159876
19	7.326	446	57.6	0.612335	4	3.91043	13.6	10.9	7.326	445.8	3.6	0.60582
20	7.4	457	59	0.315713	4	2.23346	7.8	6.2	7.4	457.3	4.5	0.280126
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.512	475	61.4	0.469663	3	2.62841	9.2	7.3	7.512	474.8	3.1	0.471657
22	7.562	483	62.4	0.134801	3	0.634696	2.2	1.8	7.562	482.9	2.6	0.138292
23	7.811	523	67.6	0.217872	3	1.24571	4.3	3.5	7.811	523	3.4	0.209509
24	7.898	537	69.4	0.505853	3	2.64041	9.2	7.4	7.898	537.6	2.9	0.511244
25	8.366	625	80.7	0.0147361	3	0.0700332	0.2	0.2	8.366	624.9	2.6	0.0152338
26	8.42	637	82.3	0.023718	3	0.129497	0.5	0.4	8.42	636.5	3	0.0240758
27	8.47	649	83.9	0.0481153	3	0.227863	0.8	0.6	8.47	648.5	3.3	0.038769
28	8.56	674	87.1	0.0753539	14	1.96018	6.8	5.5	8.56	668.7	14.3	0.0770869
29	8.685	702	90.7	0.0368463	6	0.315948	1.1	0.9	8.685	704.3	6	0.0299324
30	9.306	755	97.5	0.0565359	6	0.607582	2.1	1.7	9.306	753.3	6	0.0568318

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	18	2.3	0.050168	4	0.385126	4.8	3.2	4.55	18.2	4.5	0.0484541
2	5.2	114	14.7	0.0777099	6	0.808413	10	6.8	5.2	113.7	6	0.0759088
3	5.85	212	27.4	0.0482524	4	0.311678	3.9	2.6	5.85	211.7	3.8	0.0468575
4	6.55	352	45.5	0.170064	6	1.77893	22	14.9	6.55	351.5	6.3	0.160364
5	6.85	385	49.7	0.0561101	5	0.424989	5.3	3.5	6.85	384.8	4.7	0.0514335
6	7.35	446	57.6	0.126778	3	0.751119	9.3	6.3	7.35	445.5	3.3	0.128196
7	8.15	573	74	0.116987	6	1.33004	16.5	11.1	8.15	572.5	6.5	0.115805
8	8.45	640	82.7	0.125283	6	1.36945	16.9	11.4	8.45	639.6	6.2	0.124249
9	8.65	696	89.9	0.123478	4	0.782308	9.7	6.5	8.65	695.3	3.6	0.121502
10	9.3	751	97	0.0245386	3	0.139263	1.7	1.2	9.3	751.2	3	0.025797

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.4	0.0392762	3	0.237159	1	0.9	4.55	19.6	3.3	0.0399469
2	5.109	99	12.8	0.0373218	6	0.38748	1.6	1.5	5.109	100	6.3	0.0348752
3	5.2	112	14.4	0.083178	5	0.698111	3	2.7	5.2	111.4	4.9	0.0805969
4	5.434	152	19.6	0.0958608	3	0.485679	2.1	1.9	5.434	152.1	2.9	0.0954478
5	5.499	163	21	0.0553762	2	0.223634	0.9	0.9	5.499	162.7	2.3	0.0556874
6	5.668	192	24.7	0.0157321	10	0.2274	1	0.9	5.668	191.3	10.4	0.0123185
7	5.721	201	25.9	0.0087597	1	0.0147552	0.1	0.1	5.721	200	1.5	0.0056522
8	5.774	210	27.1	0.0164535	3	0.0759947	0.3	0.3	5.774	210.5	2.8	0.0152753
9	5.85	223	28.7	0.0288011	4	0.187512	0.8	0.7	5.85	222.9	3.5	0.0299609
10	5.899	232	29.9	0.0119274	3	0.066631	0.3	0.3	5.899	231.1	3.1	0.0120012
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.176	283	36.5	0.0353761	6	0.306116	1.3	1.2	6.176	282.2	6	0.0285723
12	6.329	311	40.1	0.0431279	4	0.261869	1.1	1	6.329	311.1	3.7	0.0397644
13	6.421	328	42.3	0.380648	3	1.73876	7.4	6.7	6.421	328.4	2.5	0.386969
14	6.487	340	43.8	0.0408608	3	0.22872	1	0.9	6.487	340.8	3	0.0434554
15	6.535	349	45	0.214078	4	1.47269	6.2	5.7	6.535	349.9	4.3	0.195106
16	6.616	359	46.3	0.394135	2	1.50465	6.4	5.8	6.616	359.4	2.2	0.384701
17	6.778	377	48.6	0.048784	3	0.274666	1.2	1.1	6.778	375.9	3.5	0.0448924
18	6.85	385	49.6	0.0421788	5	0.415881	1.8	1.6	6.85	383.9	5.2	0.0453428
19	7.14	421	54.3	0.0905716	5	0.689359	2.9	2.7	7.14	421.5	4.7	0.0825326
20	7.229	432	55.7	0.0848528	4	0.554835	2.3	2.1	7.229	431.2	3.7	0.0845972
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.363	449	57.9	0.548852	4	3.57484	15.1	13.8	7.363	448.7	3.7	0.551653
22	7.433	460	59.3	0.209917	4	1.37692	5.8	5.3	7.433	459.7	3.9	0.196736
23	7.54	477	61.5	0.368706	3	2.00198	8.5	7.7	7.54	476.8	3	0.374623
24	7.579	483	62.2	0.0963591	3	0.472555	2	1.8	7.579	483.7	2.9	0.0906988
25	7.813	520	67	0.118881	3	0.72197	3.1	2.8	7.813	520.6	3.5	0.117764
26	7.915	536	69.1	0.401844	3	2.3873	10.1	9.2	7.915	536.1	3.5	0.388426
27	8.15	573	73.8	0.0634449	6	0.673038	2.8	2.6	8.15	572.7	5.9	0.0639261
28	8.313	611	78.7	0.0211314	4	0.136702	0.6	0.5	8.313	611.8	4	0.0192418
29	8.364	623	80.3	0.0325516	4	0.263747	1.1	1	8.364	622	4.5	0.0330752
30	8.45	643	82.9	0.0534992	7	0.649534	2.8	2.5	8.45	641.8	7.2	0.0506367
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.537	667	86	0.0362956	9	0.543354	2.3	2.1	8.537	665.1	9	0.0339007
32	8.65	698	89.9	0.0619965	4	0.430688	1.8	1.7	8.65	698	4	0.0608287
33	9.3	755	97.3	0.0401344	5	0.332632	1.4	1.3	9.3	754.1	4.6	0.0408771

PeakCounter: 33

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.139	98	12.6	0.0447701	3	0.256157	0.9	0.7	5.139	98	3.2	0.0453109
2	5.215	109	14.1	0.0827876	5	0.755365	2.6	2.1	5.215	109.2	5.5	0.0776425
3	5.476	153	19.7	0.133308	3	0.682084	2.3	1.9	5.476	153.2	2.9	0.133096
4	5.547	165	21.3	0.0773358	2	0.290506	1	0.8	5.547	165	2.1	0.0790973
5	5.743	198	25.5	0.0196873	2	0.0931285	0.3	0.3	5.743	197.1	2.2	0.0241425
6	5.779	204	26.3	0.0269789	3	0.158775	0.5	0.4	5.779	204	3.4	0.0262041
7	5.903	226	29.2	0.064537	3	0.387696	1.3	1.1	5.903	225.4	3.4	0.0642148
8	5.934	232	29.9	0.0184993	4	0.130092	0.4	0.4	5.934	233.2	4.2	0.0173471
9	6.034	251	32.4	0.0119155	4	0.07047	0.2	0.2	6.034	250.7	4.2	0.0095464
10	6.196	282	36.4	0.0356437	5	0.260586	0.9	0.7	6.196	281.7	4.6	0.0317793
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.338	309	39.9	0.0629946	4	0.410669	1.4	1.2	6.338	308.9	4	0.0573034
12	6.428	326	42.1	0.43078	3	2.05355	7	5.8	6.428	325.7	2.6	0.440549
13	6.485	337	43.5	0.0594468	3	0.329415	1.1	0.9	6.485	336.1	2.6	0.072328
14	6.533	346	44.6	0.222272	3	1.22489	4.2	3.4	6.533	345.7	3.3	0.210607
15	6.611	356	45.9	0.406529	2	1.75825	6	4.9	6.611	355.8	2.5	0.398873
16	6.749	371	47.9	0.0521115	5	0.434659	1.5	1.2	6.749	372.8	4.9	0.0498952
17	7.155	421	54.3	0.129285	4	0.812992	2.8	2.3	7.155	420.9	3.7	0.125284
18	7.225	430	55.5	0.133843	4	0.89583	3	2.5	7.225	429.8	3.8	0.133237
19	7.356	447	57.7	0.600056	4	3.70563	12.6	10.4	7.356	446.9	3.6	0.584091
20	7.432	459	59.2	0.277599	5	2.02228	6.9	5.7	7.432	458.8	4.7	0.245128
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.54	476	61.4	0.418268	3	2.45465	8.3	6.9	7.54	476.4	3.3	0.423995
22	7.59	484	62.5	0.111225	4	0.659602	2.2	1.9	7.59	484.2	3.5	0.106147
23	7.818	520	67.1	0.157933	3	0.943037	3.2	2.6	7.818	520.5	3.3	0.160324
24	7.913	535	69	0.428634	3	2.56214	8.7	7.2	7.913	534.8	3.3	0.434037
25	8.348	619	79.9	0.0119092	3	0.059245	0.2	0.2	8.348	618.2	2.8	0.0117548
26	8.399	631	81.4	0.0248357	3	0.158059	0.5	0.4	8.399	630.9	3.5	0.0256849
27	8.457	645	83.2	0.0426289	4	0.313123	1.1	0.9	8.457	644.4	4.2	0.0417264
28	8.56	673	86.8	0.106346	18	3.15465	10.7	8.9	8.56	673.7	18	0.0988807
29	8.667	699	90.2	0.0918289	12	1.29655	4.4	3.6	8.667	705	12.4	0.0592156
30	9.3	754	97.3	0.0741215	9	1.11971	3.8	3.1	9.3	754.1	9	0.0702405

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	15	1.9	0.0641668	3	0.349219	3.8	2.8	4.55	14.7	3	0.0651438
2	5.2	102	13.2	0.0731366	5	0.553475	6.1	4.4	5.2	102.1	4.7	0.0667066
3	5.85	210	27.1	0.0402376	4	0.247809	2.7	2	5.85	209.7	3.8	0.0370658
4	6.55	348	44.9	0.131289	7	1.51244	16.6	12.1	6.55	346.6	7	0.122315
5	6.85	380	49	0.044798	5	0.386161	4.2	3.1	6.85	379.6	5.4	0.0402365
6	7.35	446	57.5	0.0889288	4	0.582279	6.4	4.7	7.35	446.4	3.7	0.0892252
7	8.15	573	73.9	0.154178	6	1.71874	18.8	13.8	8.15	572.4	6.4	0.151886
8	8.45	644	83.1	0.17556	6	1.8482	20.2	14.8	8.45	643.9	6	0.174198
9	8.65	698	90.1	0.136425	4	0.879257	9.6	7	8.65	698.1	3.7	0.135154
10	9.3	754	97.3	0.0498866	12	1.0586	11.6	8.5	9.3	751.1	12.4	0.0483421

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 123

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.400 5.200 7.300 5.850 3.200 6.550 16.600 6.850 6.600 7.350 8.900 8.150 19.000 8.450 23.100 8.650 10.100 9.300 0.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
5.114 1.300 5.197 2.800 5.487 3.000 5.557 1.200 5.746 0.400 5.784 0.900 5.908 2.100 5.949 0.500 6.045 0.300 6.213 1.000 6.345 1.000 6.421 7.000 6.482 2.000 6.522 4.000 6.601 5.000 6.748 2.900 7.120 3.500 7.191 3.300 7.326 13.600 7.400 7.800 7.512 9.200 7.562 2.200 7.811 4.300 7.898 9.200 8.366 0.200 8.420 0.500 8.470 0.800 8.560 6.800 8.685 1.100 9.306 2.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 30 Return to Lane Navigator
4.550 4.800 5.200 10.000 5.850 3.900 6.550 22.000 6.850 5.300 7.350 9.300 8.150 16.500 8.450 16.900 8.650 9.700 9.300 1.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 1.000 5.109 1.600 5.200 3.000 5.434 2.100 5.499 0.900 5.668 1.000 5.721 0.100 5.774 0.300 5.850 0.800 5.899 0.300 6.176 1.300 6.329 1.100 6.421 7.400 6.487 1.000 6.535 6.200 6.616 6.400 6.778 1.200 6.850 1.800 7.140 2.900 7.229 2.300 7.363 15.100 7.433 5.800 7.540 8.500 7.579 2.000 7.813 3.100 7.915 10.100 8.150 2.800 8.313 0.600 8.364 1.100 8.450 2.800 8.537 2.300 8.650 1.800 9.300 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 33 Return to Lane Navigator
5.139 0.900 5.215 2.600 5.476 2.300 5.547 1.000 5.743 0.300 5.779 0.500 5.903 1.300 5.934 0.400 6.034 0.200 6.196 0.900 6.338 1.400 6.428 7.000 6.485 1.100 6.533 4.200 6.611 6.000 6.749 1.500 7.155 2.800 7.225 3.000 7.356 12.600 7.432 6.900 7.540 8.300 7.590 2.200 7.818 3.200 7.913 8.700 8.348 0.200 8.399 0.500 8.457 1.100 8.560 10.700 8.667 4.400 9.300 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 30 Return to Lane Navigator
4.550 3.800 5.200 6.100 5.850 2.700 6.550 16.600 6.850 4.200 7.350 6.400 8.150 18.800 8.450 20.200 8.650 9.600 9.300 11.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Chub Mackerel ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Chub Mackerel ---- Go to Display Gel Lane Navigator.