RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19930816gel04
Unique Web Code: rsg01Srr
Tissue Aliquot Code: lutjcamp 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	4.2	0.198615	9	3.07905	10.3	7.9	3.5	48.4	9.2	0.189661
2	4.55	191	17.1	0.340135	4	2.30523	7.7	5.9	4.55	190.7	4.2	0.311119
3	5.2	265	23.7	0.431034	4	3.06331	10.3	7.9	5.2	264.6	4.1	0.425088
4	5.85	410	36.7	0.574468	4	3.74633	12.6	9.6	5.85	409.9	3.7	0.574392
5	6.55	573	51.3	0.376825	4	2.86554	9.6	7.4	6.55	572.7	4.5	0.35975
6	6.85	612	54.8	0.207332	6	1.90993	6.4	4.9	6.85	612.8	5.6	0.191282
7	7.35	704	63	0.390653	6	3.77278	12.6	9.7	7.35	702.8	5.8	0.369415
8	8.15	867	77.6	0.0795775	10	1.39411	4.7	3.6	8.15	865.4	10.2	0.0774556
9	8.45	928	83.1	0.12921	7	1.47113	4.9	3.8	8.45	928.5	6.6	0.126287
10	8.65	971	86.9	0.0858351	6	0.853381	2.9	2.2	8.65	969.8	5.7	0.0838636
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1094	97.9	0.394733	9	5.38824	18.1	13.9	9.3	1091.8	8.7	0.348461

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	41	3.7	0.185425	8	2.48091	4	3.4	3.5	42.9	8.1	0.172666
2	3.757	77	6.9	0.114543	6	1.30336	2.1	1.8	3.757	76.6	6.4	0.115675
3	4.279	150	13.5	0.652953	5	5.80963	9.4	7.9	4.279	150.3	4.9	0.670032
4	4.55	188	16.9	0.269016	4	1.96493	3.2	2.7	4.55	188.7	4.5	0.247969
5	4.94	233	21	0.23006	5	1.81999	3	2.5	4.94	233.5	4.7	0.216914
6	5.2	263	23.7	0.292291	4	2.13764	3.5	2.9	5.2	263	4.3	0.282585
7	5.232	270	24.3	0.0802726	2	0.345893	0.6	0.5	5.232	271.4	2.4	0.0829514
8	5.304	286	25.7	0.581264	3	2.686	4.4	3.6	5.304	286.4	2.7	0.564113
9	5.62	356	32	0.679119	4	5.36793	8.7	7.3	5.62	356	4.3	0.702014
10	5.85	407	36.6	0.436027	3	2.66425	4.3	3.6	5.85	406.9	3.4	0.439119
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.969	434	39	0.834121	4	5.64461	9.2	7.6	5.969	434	3.7	0.86341
12	6.061	455	40.9	0.593364	3	3.45682	5.6	4.7	6.061	455.1	3.4	0.566382
13	6.55	566	50.9	0.22542	5	1.78525	2.9	2.4	6.55	564.6	4.9	0.207447
14	6.684	583	52.4	0.503216	4	3.4375	5.6	4.7	6.684	583.9	3.9	0.491295
15	6.771	594	53.4	0.443038	4	2.28809	3.7	3.1	6.771	592.8	3.7	0.348509
16	6.85	604	54.3	0.0683227	5	0.703465	1.1	1	6.85	603.8	5.4	0.0731107
17	7.16	661	59.4	0.157934	6	1.56639	2.5	2.1	7.16	659.9	6.2	0.142631
18	7.35	696	62.6	0.353129	5	3.30845	5.4	4.5	7.35	695.4	5.4	0.345195
19	7.855	797	71.7	0.152195	8	2.02222	3.3	2.7	7.855	797.2	8.3	0.137792
20	8.15	856	77	0.421424	4	2.30891	3.7	3.1	8.15	856.5	3.7	0.351831
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	916	82.4	0.115474	8	1.59937	2.6	2.2	8.45	916.4	8.4	0.107514
22	8.65	961	86.4	0.105979	6	1.07843	1.7	1.5	8.65	961.2	6	0.100963
23	8.841	998	89.7	0.0480155	14	1.31634	2.1	1.8	8.841	995.8	14.5	0.051297
24	9.3	1087	97.8	0.369147	8	4.57361	7.4	6.2	9.3	1084.5	7.8	0.33249

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.758	76	6.8	0.116384	7	1.32114	3.3	2.7	3.758	75.4	6.7	0.110951
2	4.267	147	13.2	0.618741	5	5.493	13.6	11.1	4.267	147.2	4.8	0.641173
3	4.931	231	20.8	0.179587	5	1.3876	3.4	2.8	4.931	231	4.6	0.16932
4	5.229	269	24.2	0.11213	5	0.907825	2.2	1.8	5.229	268.4	5.4	0.0949009
5	5.293	283	25.5	0.617505	3	3.04167	7.5	6.1	5.293	283.6	2.8	0.608141
6	5.605	352	31.7	0.677382	4	5.01579	12.4	10.1	5.605	352.2	4	0.699286
7	5.827	401	36.1	0.0476298	7	0.49328	1.2	1	5.827	402.9	7.4	0.0377652
8	5.947	428	38.6	0.880169	4	5.57439	13.8	11.3	5.947	428.3	3.6	0.87731
9	6.039	449	40.5	0.559932	4	3.30796	8.2	6.7	6.039	448.6	3.5	0.527119
10	6.515	557	50.2	0.106028	2	0.409249	1	0.8	6.515	556.6	2.2	0.104746
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.682	582	52.4	0.442222	9	7.17596	17.8	14.5	6.682	577.5	9.5	0.426321
12	7.084	647	58.3	0.121779	8	1.66037	4.1	3.4	7.084	645.5	7.8	0.120633
13	7.294	686	61.8	0.0689704	5	0.658665	1.6	1.3	7.294	685	4.8	0.0770403
14	7.815	790	71.2	0.138495	6	1.31641	3.3	2.7	7.815	790	5.7	0.130844
15	8.822	994	89.5	0.106798	15	2.66017	6.6	5.4	8.822	994.3	14.6	0.102795

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.6	0.220838	9	3.42253	10.4	8	3.5	42.1	9	0.214663
2	4.55	186	16.8	0.349712	5	2.72245	8.2	6.4	4.55	186.5	4.7	0.330267
3	5.2	263	23.7	0.470549	4	3.65727	11.1	8.6	5.2	262.4	4.4	0.463959
4	5.85	406	36.6	0.623642	4	4.07317	12.3	9.5	5.85	406.4	3.6	0.645297
5	6.55	565	50.9	0.447625	4	3.38998	10.3	7.9	6.55	565	4.5	0.428082
6	6.85	604	54.5	0.235474	6	2.20206	6.7	5.2	6.85	604.3	5.7	0.216308
7	7.35	698	62.9	0.385737	6	3.8571	11.7	9	7.35	696.6	5.9	0.366595
8	8.15	860	77.5	0.0878021	10	1.50438	4.5	3.5	8.15	859.4	10.1	0.0837163
9	8.45	921	83	0.145156	7	1.68587	5.1	3.9	8.45	921.1	6.7	0.141311
10	8.65	962	86.7	0.103962	6	1.06149	3.2	2.5	8.65	961.4	6.1	0.0989356
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1086	97.9	0.43049	8	5.4912	16.6	12.8	9.3	1083.8	8.2	0.378802

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	36	3.2	0.175792	8	2.27534	4	3.4	3.5	37.2	7.8	0.164556
2	3.74	68	6.1	0.087546	6	0.910833	1.6	1.4	3.74	67.6	6	0.0858133
3	4.28	140	12.6	0.575858	5	4.94562	8.7	7.3	4.28	140	4.7	0.589648
4	4.55	176	15.9	0.264902	4	1.79224	3.2	2.7	4.55	176.5	4.2	0.242426
5	4.911	216	19.5	0.153129	7	1.56975	2.8	2.3	4.911	217	6.8	0.131205
6	5.2	248	22.4	0.277482	4	2.00451	3.5	3	5.2	247.6	4.1	0.274066
7	5.228	254	22.9	0.0600525	2	0.292985	0.5	0.4	5.228	256.4	2.4	0.0683694
8	5.301	270	24.4	0.52886	2	2.19987	3.9	3.3	5.301	270.5	2.4	0.527905
9	5.62	339	30.6	0.63941	4	4.85638	8.5	7.2	5.62	339.1	4.1	0.661718
10	5.85	389	35.1	0.436585	3	2.60288	4.6	3.9	5.85	389.3	3.3	0.439321
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.973	418	37.7	0.820146	4	5.29226	9.3	7.8	5.973	418.2	3.6	0.836584
12	6.066	440	39.7	0.587757	3	3.33907	5.9	5	6.066	439.3	3.4	0.557492
13	6.55	554	50	0.232866	3	1.38381	2.4	2.1	6.55	553.7	3.4	0.231082
14	6.7	572	51.6	0.476795	4	3.47084	6.1	5.1	6.7	573.6	4.2	0.463779
15	6.783	582	52.5	0.400593	2	1.41567	2.5	2.1	6.783	581.6	2.3	0.345096
16	6.85	590	53.2	0.0544269	6	0.606103	1.1	0.9	6.85	593.1	6	0.056945
17	7.155	648	58.5	0.0831573	7	0.998282	1.8	1.5	7.155	646.1	6.9	0.0814513
18	7.35	685	61.8	0.344986	5	3.06912	5.4	4.6	7.35	683.8	5.1	0.336298
19	7.865	786	70.9	0.161455	8	1.98633	3.5	2.9	7.865	786.5	8	0.14011
20	8.15	842	76	0.0791847	20	2.92939	5.2	4.3	8.15	850.1	20.1	0.0820766
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	904	81.6	0.113447	6	1.19865	2.1	1.8	8.45	904.5	5.9	0.113992
22	8.65	951	85.8	0.110153	6	1.09535	1.9	1.6	8.65	950.9	5.8	0.107179
23	8.824	986	89	0.0770263	14	1.77685	3.1	2.6	8.824	987.8	14.3	0.0700765
24	9.3	1082	97.7	0.404307	7	4.85591	8.5	7.2	9.3	1080.4	7.4	0.369896

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.76	70	6.3	0.125158	7	1.6647	4	3.1	3.76	70.3	7.4	0.127237
2	4.362	150	13.6	0.646581	5	6.24689	14.9	11.8	4.362	149.5	5.3	0.663531
3	5.11	237	21.4	0.172014	5	1.42201	3.4	2.7	5.11	236.5	4.9	0.162565
4	5.32	273	24.7	0.125752	8	1.49843	3.6	2.8	5.32	272.7	7.7	0.10944
5	5.385	287	25.9	0.696615	3	3.13934	7.5	5.9	5.385	286.9	2.7	0.659216
6	5.677	350	31.6	0.68774	4	5.05666	12.1	9.5	5.677	350.1	4	0.705564
7	5.911	402	36.3	0.0402533	6	0.74332	1.8	1.4	5.911	399.7	6	0.0696984
8	6.004	424	38.3	0.81743	3	5.02128	12	9.5	6.004	424.3	3.5	0.816251
9	6.081	442	40	0.622329	3	3.51389	8.4	6.6	6.081	442	3.4	0.587776
10	6.533	549	49.6	0.116007	2	0.408663	1	0.8	6.533	548.6	2.4	0.0976392
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.661	567	51.3	0.485143	5	3.76787	9	7.1	6.661	566.9	4.6	0.461819
12	6.724	575	52	0.466557	5	3.19346	7.6	6	6.724	575.4	5	0.362609
13	7.117	641	58	0.156846	8	2.14283	5.1	4	7.117	639.7	7.8	0.154634
14	7.289	673	60.8	0.11197	5	0.967366	2.3	1.8	7.289	673.1	5	0.110259
15	7.828	778	70.3	0.119896	6	1.24382	3	2.3	7.828	778.5	6.1	0.115608
16	8.844	988	89.3	0.0807877	13	1.87215	4.5	3.5	8.844	987.2	13.3	0.0792002

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	35	3.2	0.246645	9	3.98957	11.1	8.3	3.5	36.5	9.3	0.242619
2	4.55	174	15.7	0.37112	4	2.4332	6.8	5.1	4.55	174.4	4	0.344022
3	5.2	246	22.3	0.463097	4	3.188	8.9	6.6	5.2	245.6	4	0.451126
4	5.85	386	34.9	0.617905	4	4.11564	11.5	8.6	5.85	386.5	3.7	0.633767
5	6.55	552	50	0.519035	4	3.87028	10.8	8.1	6.55	551.6	4.4	0.497549
6	6.85	592	53.6	0.312296	6	2.88854	8.1	6	6.85	592.5	5.6	0.292316
7	7.35	684	61.9	0.507911	6	4.81471	13.4	10	7.35	683	5.5	0.492664
8	8.15	840	76	0.115169	9	1.75065	4.9	3.6	8.15	839.8	8.9	0.111325
9	8.45	906	82	0.135242	6	1.42958	4	3	8.45	904.9	6	0.135138
10	8.65	948	85.8	0.109467	6	1.07245	3	2.2	8.65	947.3	5.7	0.106622
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1075	97.3	0.533695	7	6.31082	17.6	13.1	9.3	1073.6	7	0.505259

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 112

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 10.300 4.550 7.700 5.200 10.300 5.850 12.600 6.550 9.600 6.850 6.400 7.350 12.600 8.150 4.700 8.450 4.900 8.650 2.900 9.300 18.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 4.000 3.757 2.100 4.279 9.400 4.550 3.200 4.940 3.000 5.200 3.500 5.232 0.600 5.304 4.400 5.620 8.700 5.850 4.300 5.969 9.200 6.061 5.600 6.550 2.900 6.684 5.600 6.771 3.700 6.850 1.100 7.160 2.500 7.350 5.400 7.855 3.300 8.150 3.700 8.450 2.600 8.650 1.700 8.841 2.100 9.300 7.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 24 Return to Lane Navigator
3.758 3.300 4.267 13.600 4.931 3.400 5.229 2.200 5.293 7.500 5.605 12.400 5.827 1.200 5.947 13.800 6.039 8.200 6.515 1.000 6.682 17.800 7.084 4.100 7.294 1.600 7.815 3.300 8.822 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 15 Return to Lane Navigator
3.500 10.400 4.550 8.200 5.200 11.100 5.850 12.300 6.550 10.300 6.850 6.700 7.350 11.700 8.150 4.500 8.450 5.100 8.650 3.200 9.300 16.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
3.500 4.000 3.740 1.600 4.280 8.700 4.550 3.200 4.911 2.800 5.200 3.500 5.228 0.500 5.301 3.900 5.620 8.500 5.850 4.600 5.973 9.300 6.066 5.900 6.550 2.400 6.700 6.100 6.783 2.500 6.850 1.100 7.155 1.800 7.350 5.400 7.865 3.500 8.150 5.200 8.450 2.100 8.650 1.900 8.824 3.100 9.300 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 17 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 19 PeakCount: 24 Return to Lane Navigator
3.760 4.000 4.362 14.900 5.110 3.400 5.320 3.600 5.385 7.500 5.677 12.100 5.911 1.800 6.004 12.000 6.081 8.400 6.533 1.000 6.661 9.000 6.724 7.600 7.117 5.100 7.289 2.300 7.828 3.000 8.844 4.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 18 LANE TYPE: Specimen ACTUAL GEL LANE: 20 PeakCount: 16 Return to Lane Navigator
3.500 11.100 4.550 6.800 5.200 8.900 5.850 11.500 6.550 10.800 6.850 8.100 7.350 13.400 8.150 4.900 8.450 4.000 8.650 3.000 9.300 17.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 19 LANE TYPE: pI Markers ACTUAL GEL LANE: 21 PeakCount: 11 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.