RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Quillback Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes maliger
FDA Seafood List Common Name: Quillback Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19940913gel03
Unique Web Code: qbh01Srr
Tissue Aliquot Code: sebamali I94-002 #06a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	80	9.6	0.0839434	5	0.736446	9.5	7.3	4.55	79.6	5.3	0.0786597
2	5.2	174	20.9	0.103979	4	0.763592	9.8	7.5	5.2	173.4	4.4	0.0985921
3	5.85	282	33.9	0.128232	5	1.00227	12.9	9.9	5.85	282.3	4.6	0.122549
4	6.55	414	49.8	0.101264	8	1.33431	17.2	13.2	6.55	414.5	7.8	0.0965891
5	7.35	522	62.7	0.044062	6	0.441812	5.7	4.4	7.35	521.6	6.1	0.0409398
6	8.15	647	77.8	0.0641014	8	0.927974	12	9.2	8.15	645.6	8.4	0.0620932
7	8.45	709	85.2	0.0877541	7	1.15325	14.9	11.4	8.45	708.9	7.4	0.0880859
8	8.65	758	91.1	0.059323	5	0.45217	5.8	4.5	8.65	757.1	4.5	0.0565536
9	9.3	803	96.5	0.0994166	6	0.950012	12.2	9.4	9.3	802.6	5.9	0.0910805

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.246	36	4.3	0.161771	7	2.02402	9.2	6	4.246	36.8	7.4	0.154235
2	4.536	77	9.2	0.0608559	8	0.757358	3.4	2.3	4.536	75.5	8.1	0.0524854
3	4.691	99	11.9	0.247042	5	1.57719	7.2	4.7	4.691	99.7	4.6	0.195556
4	4.882	126	15.1	0.16645	23	7.14012	32.5	21.2	4.882	124.5	23.3	0.172537
5	5.905	290	34.8	0.571899	3	3.0983	14.1	9.2	5.905	290.2	3.1	0.556206
6	5.988	306	36.7	0.252221	7	2.93492	13.3	8.7	5.988	304.5	6.8	0.243834
7	6.124	332	39.8	0.077112	3	0.387532	1.8	1.2	6.124	332.2	3	0.0740465
8	6.161	339	40.6	0.0412946	5	0.387062	1.8	1.2	6.161	340.3	5	0.0435259
9	6.213	349	41.8	0.20511	2	0.742995	3.4	2.2	6.213	348.7	2.1	0.1984
10	6.349	375	45	0.06864	5	0.622633	2.8	1.9	6.349	375.6	5.4	0.0655703
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.474	399	47.8	0.0504336	3	0.173933	0.8	0.5	6.474	397.2	3.4	0.0284446
12	6.516	407	48.8	0.0428074	9	0.622846	2.8	1.9	6.516	404.4	8.9	0.0395297
13	7.369	524	62.8	0.0395901	9	0.566312	2.6	1.7	7.369	526.6	8.6	0.0372943
14	7.619	564	67.6	0.100253	6	0.963665	4.4	2.9	7.619	564.2	6.2	0.0876316

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	80	9.5	0.0644636	6	0.654193	8.9	6.7	4.55	80.4	6.2	0.0594653
2	5.2	170	20.3	0.0893458	5	0.79959	10.9	8.1	5.2	170.1	5.1	0.0883108
3	5.85	279	33.3	0.118456	5	1.02107	13.9	10.4	5.85	278.6	5.1	0.113152
4	6.55	415	49.5	0.12457	7	1.47084	20	15	6.55	415.1	7.2	0.115958
5	7.35	522	62.3	0.0337384	7	0.392623	5.3	4	7.35	523.1	7	0.0317977
6	8.15	653	77.9	0.0616597	8	0.840545	11.4	8.6	8.15	652	8	0.0590732
7	8.45	714	85.2	0.0579678	8	0.742951	10.1	7.6	8.45	712.6	7.5	0.0557456
8	8.65	759	90.6	0.0437876	5	0.394922	5.4	4	8.65	759	5.4	0.0412465
9	9.3	807	96.3	0.0994412	6	1.02728	14	10.5	9.3	806.2	6.3	0.0927055

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.353	49	5.9	0.0995778	9	1.5021	7.5	5.5	4.353	50	8.8	0.0963488
2	4.614	91	10.9	0.033468	6	0.344744	1.7	1.3	4.614	90.4	5.9	0.032935
3	4.758	114	13.6	0.118695	12	2.40685	12	8.9	4.758	117	12.4	0.109782
4	4.926	141	16.8	0.172983	17	5.21145	25.9	19.2	4.926	142.7	16.8	0.174935
5	5.2	185	22.1	0.0217693	4	0.118419	0.6	0.4	5.2	187.2	4.4	0.0152107
6	5.85	291	34.8	0.0363808	6	0.324665	1.6	1.2	5.85	291.4	5.5	0.033117
7	5.917	303	36.2	0.346656	4	2.42016	12	8.9	5.917	303	4	0.338642
8	5.995	317	37.9	0.168264	7	2.20953	11	8.2	5.995	317.5	7.4	0.168589
9	6.157	346	41.3	0.0622899	7	0.681679	3.4	2.5	6.157	347	6.6	0.0583341
10	6.235	360	43	0.11145	4	0.756437	3.8	2.8	6.235	359.1	4.1	0.104838
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.369	384	45.9	0.0459796	5	0.412422	2.1	1.5	6.369	383.2	5.1	0.0457446
12	6.503	408	48.7	0.0438051	6	0.285747	1.4	1.1	6.503	404.6	5.5	0.0292638
13	6.543	415	49.6	0.0482407	8	0.698993	3.5	2.6	6.543	415.6	8.3	0.0473696
14	7.35	529	63.2	0.0378983	10	0.562377	2.8	2.1	7.35	530.2	9.8	0.0324574
15	7.604	570	68.1	0.0314028	5	0.276544	1.4	1	7.604	569.5	5.3	0.0291867
16	8.15	658	78.6	0.0242216	14	0.890029	4.4	3.3	8.15	648.8	13.9	0.0361367
17	8.45	725	86.6	0.0215578	6	0.22418	1.1	0.8	8.45	723.2	6	0.0209928
18	8.65	761	90.9	0.0196957	5	0.159681	0.8	0.6	8.65	760.1	4.8	0.0187143
19	9.3	809	96.7	0.0376222	8	0.617565	3.1	2.3	9.3	805.6	7.7	0.0454964

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.274	40	4.8	0.128688	8	1.69229	9.2	5	4.274	41.7	7.7	0.124024
2	4.529	80	9.5	0.146105	8	2.01568	11	5.9	4.529	79.6	7.9	0.143406
3	4.669	102	12.2	0.196775	4	1.3511	7.4	4	4.669	102	3.8	0.198534
4	4.86	132	15.7	0.0619185	14	1.48751	8.1	4.4	4.86	131	14.1	0.0594519
5	5.912	301	35.9	0.618088	3	3.27192	17.8	9.7	5.912	301.3	3	0.606055
6	5.993	316	37.7	0.328383	6	3.51205	19.1	10.4	5.993	314.5	6	0.329054
7	6.122	340	40.5	0.102332	3	0.312638	1.7	0.9	6.122	339.4	2.6	0.0691669
8	6.154	346	41.2	0.0602935	8	0.820856	4.5	2.4	6.154	345.5	7.9	0.0586957
9	6.197	354	42.2	0.223059	2	0.868399	4.7	2.6	6.197	354.4	2.3	0.211962
10	6.342	381	45.4	0.0678933	6	0.691121	3.8	2	6.342	382	5.8	0.0670736
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.487	408	48.6	0.0707331	5	0.481566	2.6	1.4	6.487	406.5	4.6	0.0590066
12	6.536	417	49.7	0.0575998	6	0.614882	3.3	1.8	6.536	415.7	6.5	0.0534971
13	7.363	531	63.3	0.0474837	9	0.662853	3.6	2	7.363	534.2	9.1	0.0412748
14	7.633	574	68.4	0.0786009	4	0.596285	3.2	1.8	7.633	574.1	4.4	0.077247

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	86	10.2	0.0790995	5	0.704927	8.3	6.7	4.55	86.3	5.5	0.0726205
2	5.2	186	22.1	0.0921648	5	0.816214	9.6	7.8	5.2	185.2	5.3	0.087679
3	5.85	288	34.3	0.11914	5	1.07538	12.7	10.3	5.85	287.7	5.4	0.113359
4	6.55	423	50.4	0.0917343	9	1.46293	17.3	14	6.55	423.2	9.4	0.0878045
5	7.35	529	63	0.0395466	14	0.807184	9.5	7.7	7.35	535.6	14.4	0.0315647
6	8.15	654	77.9	0.0730785	8	0.984425	11.6	9.4	8.15	653.7	7.8	0.070941
7	8.45	715	85.1	0.083099	7	1.07689	12.7	10.3	8.45	714.1	7.5	0.0810331
8	8.65	763	90.8	0.0523225	5	0.405282	4.8	3.9	8.65	762.2	4.6	0.0499478
9	9.3	809	96.3	0.119425	6	1.1435	13.5	10.9	9.3	808.8	5.8	0.111433

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 74

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 9.500 5.200 9.800 5.850 12.900 6.550 17.200 7.350 5.700 8.150 12.000 8.450 14.900 8.650 5.800 9.300 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.246 9.200 4.536 3.400 4.691 7.200 4.882 32.500 5.905 14.100 5.988 13.300 6.124 1.800 6.161 1.800 6.213 3.400 6.349 2.800 6.474 0.800 6.516 2.800 7.369 2.600 7.619 4.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 8.900 5.200 10.900 5.850 13.900 6.550 20.000 7.350 5.300 8.150 11.400 8.450 10.100 8.650 5.400 9.300 14.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.353 7.500 4.614 1.700 4.758 12.000 4.926 25.900 5.200 0.600 5.850 1.600 5.917 12.000 5.995 11.000 6.157 3.400 6.235 3.800 6.369 2.100 6.503 1.400 6.543 3.500 7.350 2.800 7.604 1.400 8.150 4.400 8.450 1.100 8.650 0.800 9.300 3.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
4.274 9.200 4.529 11.000 4.669 7.400 4.860 8.100 5.912 17.800 5.993 19.100 6.122 1.700 6.154 4.500 6.197 4.700 6.342 3.800 6.487 2.600 6.536 3.300 7.363 3.600 7.633 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 14 Return to Lane Navigator
4.550 8.300 5.200 9.600 5.850 12.700 6.550 17.300 7.350 9.500 8.150 11.600 8.450 12.700 8.650 4.800 9.300 13.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Quillback Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Quillback Rockfish ---- Go to Display Gel Lane Navigator.