RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Petrale Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Eopsetta jordani
FDA Seafood List Common Name: Petrale Sole
FDA Seafood List Market Name: Sole (Flounder)
Unique IEF Gel Code: 19970212gel10
Unique Web Code: pth01Srr
Tissue Aliquot Code: eopsjord I94-003 #03m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	69	8.7	0.0388745	5	0.310649	6.3	4.5	4.55	69.2	4.8	0.0361622
2	5.2	166	21	0.0585747	5	0.454278	9.2	6.6	5.2	166	4.6	0.0560581
3	5.85	276	34.9	0.0611959	5	0.499224	10.1	7.3	5.85	276.2	4.6	0.061611
4	6.55	421	53.3	0.0743706	8	0.930379	18.7	13.5	6.55	420.1	7.5	0.069574
5	6.85	453	57.3	0.0212516	6	0.190698	3.8	2.8	6.85	454.6	5.6	0.0191425
6	7.35	521	65.9	0.0468118	5	0.388302	7.8	5.6	7.35	520.6	5	0.04407
7	8.15	641	81.1	0.0710726	7	0.822101	16.6	12	8.15	640.5	6.9	0.0675252
8	8.45	705	89.2	0.0854905	6	0.904143	18.2	13.1	8.45	704.9	6.1	0.0837191
9	8.65	755	95.6	0.0578949	5	0.464141	9.4	6.8	8.65	754.2	4.7	0.0554249

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.205	17	2.2	0.151014	7	1.8	5.5	4.5	4.205	17.5	6.8	0.149993
2	4.734	96	12.2	0.0844177	18	2.54022	7.8	6.3	4.734	99.9	18	0.0796219
3	5.123	154	19.5	0.0116414	2	0.0462242	0.1	0.1	5.123	154.3	2.4	0.0110651
4	5.358	192	24.3	0.0297945	5	0.212964	0.7	0.5	5.358	190.8	4.5	0.0266057
5	5.799	266	33.7	0.588136	4	4.43056	13.6	11	5.799	266	4.4	0.562354
6	5.904	286	36.2	0.2153	11	3.54793	10.9	8.8	5.904	286.7	10.8	0.184629
7	6.017	310	39.2	0.0412424	6	0.387318	1.2	1	6.017	308.7	6.1	0.0360239
8	6.064	320	40.5	0.0322323	2	0.0847087	0.3	0.2	6.064	319.9	1.9	0.0250508
9	6.106	329	41.6	0.413593	3	2.12713	6.5	5.3	6.106	329.1	2.9	0.419652
10	6.303	371	47	0.108602	5	0.856863	2.6	2.1	6.303	370	4.7	0.102638
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.341	379	48	0.103065	6	0.995618	3.1	2.5	6.341	380.4	6.1	0.0920175
12	6.393	390	49.4	0.432916	2	1.48037	4.5	3.7	6.393	389.6	2	0.411006
13	6.435	399	50.5	0.18323	6	1.92613	5.9	4.8	6.435	399.1	6.1	0.178844
14	6.83	452	57.2	0.0224428	3	0.12007	0.4	0.3	6.83	452.3	2.9	0.0231245
15	6.916	463	58.6	0.327336	3	1.71286	5.3	4.2	6.916	463.3	3	0.324679
16	6.997	474	60	0.207242	3	1.13728	3.5	2.8	6.997	473.6	3.2	0.199917
17	7.115	490	62	0.0686233	11	1.22954	3.8	3.1	7.115	491.4	11	0.0631141
18	8.682	765	96.8	0.426762	10	7.95448	24.4	19.7	8.682	763.4	10.3	0.434046

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	68	8.6	0.0429969	4	0.321711	6.1	4.5	4.55	68.5	4.2	0.0430541
2	5.2	165	20.9	0.0568614	5	0.457577	8.6	6.4	5.2	164.8	4.7	0.0551166
3	5.85	273	34.5	0.0670914	5	0.55069	10.4	7.7	5.85	272.9	4.6	0.0674299
4	6.55	426	53.9	0.072806	8	0.905107	17	12.6	6.55	424.4	7.5	0.067874
5	6.85	455	57.5	0.0198164	6	0.215431	4.1	3	6.85	455.5	6.1	0.0199447
6	7.35	523	66.1	0.0438924	5	0.384344	7.2	5.4	7.35	523.4	5	0.0430169
7	8.15	643	81.3	0.0751101	7	0.890116	16.7	12.4	8.15	642.4	7	0.0716658
8	8.45	708	89.5	0.101415	6	1.06223	20	14.8	8.45	707.9	6.1	0.0978057
9	8.65	759	96	0.064111	5	0.528134	9.9	7.4	8.65	758.8	5	0.0601395

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.252	31	3.9	0.0529392	11	0.945987	4	3.4	4.252	31.4	10.7	0.0500141
2	4.55	75	9.5	0.0761108	11	1.39606	6	5.1	4.55	77.3	10.9	0.0723663
3	5.105	157	19.9	0.00686318	3	0.0339396	0.1	0.1	5.105	155.6	2.8	0.00685245
4	5.2	171	21.6	0.0439851	4	0.290874	1.2	1.1	5.2	170.5	3.8	0.0426398
5	5.816	268	33.9	0.524143	3	3.10609	13.3	11.3	5.816	268.1	3.5	0.50574
6	5.906	285	36.1	0.218007	9	2.68899	11.5	9.8	5.906	284.4	8.6	0.176911
7	6.03	311	39.4	0.0188305	2	0.0699723	0.3	0.3	6.03	311.3	2.2	0.017991
8	6.073	320	40.5	0.0285605	3	0.132398	0.6	0.5	6.073	320.2	2.6	0.0292269
9	6.125	331	41.9	0.389235	2	1.70808	7.3	6.2	6.125	331.1	2.5	0.388638
10	6.34	376	47.6	0.112591	7	1.45527	6.2	5.3	6.34	376.4	7.5	0.109735
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.378	384	48.6	0.104813	4	0.445371	1.9	1.6	6.378	387.2	4.4	0.0572988
12	6.421	393	49.7	0.371813	2	1.1714	5	4.3	6.421	393.1	2.1	0.321679
13	6.469	403	51	0.127161	6	1.49968	6.4	5.5	6.469	400.2	5.7	0.149056
14	6.55	420	53.2	0.0247743	9	0.545968	2.3	2	6.55	414.4	9.4	0.0329289
15	6.85	452	57.2	0.0352771	4	0.244335	1	0.9	6.85	451.8	3.8	0.0363629
16	6.919	462	58.5	0.185614	3	0.862484	3.7	3.1	6.919	462.3	2.6	0.187546
17	7.003	474	60	0.104935	2	0.435908	1.9	1.6	7.003	473.7	2.3	0.105674
18	7.114	490	62	0.012985	9	0.200357	0.9	0.7	7.114	494.1	8.8	0.0128646
19	7.35	524	66.3	0.0264499	4	0.210251	0.9	0.8	7.35	524.4	4.4	0.0266725
20	8.15	645	81.6	0.031153	6	0.338366	1.4	1.2	8.15	645.8	6.1	0.03148
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	714	90.4	0.0474967	6	0.471478	2	1.7	8.45	713.2	6.1	0.0435965
22	8.697	770	97.5	0.252934	11	5.12586	21.9	18.7	8.697	765.9	11	0.263231

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.166	20	2.5	0.150745	8	1.83496	5.1	4	4.166	20.2	7.7	0.134578
2	4.662	91	11.5	0.150177	24	6.48095	17.9	14.3	4.662	84.1	24	0.152356
3	5.297	184	23.3	0.0144129	7	0.136574	0.4	0.3	5.297	183.9	7.1	0.0109282
4	5.779	263	33.3	0.581471	4	4.25948	11.8	9.4	5.779	263.7	4.2	0.56789
5	5.88	281	35.6	0.269498	9	3.70445	10.2	8.2	5.88	280.8	9	0.231083
6	5.998	306	38.7	0.0414121	12	0.666357	1.8	1.5	5.998	302.5	11.5	0.0326844
7	6.045	316	40	0.0393196	2	0.0779401	0.2	0.2	6.045	316.6	1.9	0.023194
8	6.097	327	41.4	0.424236	3	2.07039	5.7	4.6	6.097	327	2.8	0.419259
9	6.314	373	47.2	0.118281	11	2.34158	6.5	5.2	6.314	377.4	11.3	0.116742
10	6.399	391	49.5	0.403124	2	1.46715	4.1	3.2	6.399	390.8	2.4	0.351229
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.437	399	50.5	0.180458	6	1.71121	4.7	3.8	6.437	399	5.5	0.175037
12	6.788	450	57	0.0255288	3	0.148031	0.4	0.3	6.788	449.7	3.2	0.0258998
13	6.879	461	58.4	0.297483	3	1.51563	4.2	3.3	6.879	461.3	2.9	0.297716
14	6.96	472	59.7	0.190026	3	0.9588	2.7	2.1	6.96	472.4	3	0.181481
15	7.093	490	62	0.0592888	13	1.22737	3.4	2.7	7.093	492.4	12.9	0.0537509
16	8.664	764	96.7	0.380351	11	7.54991	20.9	16.6	8.664	763.2	10.9	0.391469

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	75	9.5	0.042279	4	0.324511	6.4	4.8	4.55	75.1	4.5	0.0409433
2	5.2	165	20.9	0.0564323	5	0.495464	9.8	7.4	5.2	165.2	5.2	0.0539859
3	5.85	276	34.9	0.0819841	4	0.613103	12.2	9.2	5.85	275.9	4.3	0.081296
4	6.55	426	53.9	0.0582294	7	0.70385	14	10.5	6.55	424.9	7.5	0.0532185
5	6.85	462	58.4	0.0188486	6	0.192913	3.8	2.9	6.85	461.9	6.1	0.0178054
6	7.35	526	66.5	0.0477151	5	0.409315	8.1	6.1	7.35	526.1	5	0.0465704
7	8.15	648	81.9	0.0721935	7	0.831929	16.5	12.4	8.15	647.2	6.6	0.0708537
8	8.45	711	89.9	0.096854	6	1.02199	20.3	15.3	8.45	711	6.2	0.0932533
9	8.65	762	96.3	0.0572868	5	0.449223	8.9	6.7	8.65	761.2	4.6	0.0545493

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 83

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.300 5.200 9.200 5.850 10.100 6.550 18.700 6.850 3.800 7.350 7.800 8.150 16.600 8.450 18.200 8.650 9.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.205 5.500 4.734 7.800 5.123 0.100 5.358 0.700 5.799 13.600 5.904 10.900 6.017 1.200 6.064 0.300 6.106 6.500 6.303 2.600 6.341 3.100 6.393 4.500 6.435 5.900 6.830 0.400 6.916 5.300 6.997 3.500 7.115 3.800 8.682 24.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 6.100 5.200 8.600 5.850 10.400 6.550 17.000 6.850 4.100 7.350 7.200 8.150 16.700 8.450 20.000 8.650 9.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.252 4.000 4.550 6.000 5.105 0.100 5.200 1.200 5.816 13.300 5.906 11.500 6.030 0.300 6.073 0.600 6.125 7.300 6.340 6.200 6.378 1.900 6.421 5.000 6.469 6.400 6.550 2.300 6.850 1.000 6.919 3.700 7.003 1.900 7.114 0.900 7.350 0.900 8.150 1.400 8.450 2.000 8.697 21.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.166 5.100 4.662 17.900 5.297 0.400 5.779 11.800 5.880 10.200 5.998 1.800 6.045 0.200 6.097 5.700 6.314 6.500 6.399 4.100 6.437 4.700 6.788 0.400 6.879 4.200 6.960 2.700 7.093 3.400 8.664 20.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 6.400 5.200 9.800 5.850 12.200 6.550 14.000 6.850 3.800 7.350 8.100 8.150 16.500 8.450 20.300 8.650 8.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Petrale Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Petrale Sole ---- Go to Display Gel Lane Navigator.