Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Pataganian Toothfish  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Dissostichus eleginoides
FDA Seafood List Common Name: Pataganian Toothfish
FDA Seafood List Market Name: Patagonian Toothfish
Unique IEF Gel Code: 19980129gel01
Unique Web Code: png02Srr
Tissue Aliquot Code: disseleg L97-001 #05m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
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Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5431.90.266554135.87404186.43.54613.40.248004
24.55375170.521189129.99810113.610.84.55375.411.70.480583
35.255525.10.293354157.5456410.38.25.2554.615.30.278189
45.8585938.90.50111311.62172115.812.65.85863.7130.506301
56.55120354.50.44710397.0164249.67.66.551202.39.10.434802
66.85128758.30.193702164.9416226.75.46.85128715.70.178132
77.35147166.60.297619157.48797610.28.17.351468.915.50.273002
88.15178180.70.10629173.1872874.33.58.151781.117.40.103329
98.45188985.60.198473113.9166045.34.28.451890.211.40.193497
108.65195788.60.173798103.0759994.23.38.65195810.10.171799
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3217798.60.555196108.71537411.99.59.32175.69.90.495013
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 8
LANE TYPE: Specimen
ACTUAL GEL LANE: 9
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.33528512.90.7614511519.547671915.94.335290.614.70.750192
24.59436116.40.645832910.36225810.18.44.594360.79.40.619932
34.81442119.10.08424791.2423211.214.814422.78.50.082101
44.969463210.02067480.3216190.30.34.969462.28.20.022176
55.1651523.40.074769151.8032421.81.55.16516.714.70.069267
65.24854924.90.55723287.7516147.56.35.248549.47.90.553423
75.33559126.80.20334272.4601992.425.335592.26.70.207205
85.43163728.90.36110674.09302643.35.431637.56.70.346295
95.48766430.20.07759580.98498710.85.487663.37.70.072115
105.60672132.70.4994865.5127175.44.55.606723.16.40.488453
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.67775534.30.5920111212.95345812.610.55.677759120.611039
125.75279135.90.394333149.20392897.55.752794.313.70.379599
135.8684338.30.09819761.01208510.85.86844.15.50.10351
146.12998044.50.30940242.2827122.21.96.129978.94.20.306392
156.312107348.70.126945102.2044172.11.86.3121071.4100.124761
166.353109449.70.513526108.22226186.76.3531093.310.20.453407
176.446114151.80.3471032312.73229312.410.36.4461136.723.10.310775
PeakCounter: 17
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 9
LANE TYPE: pI Markers
ACTUAL GEL LANE: 10
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5431.90.2555252310.0219312.810.73.549.523.30.243097
24.5533515.20.389964138.95147511.49.64.55338.613.10.386033
35.2509230.230538197.86865710.18.45.2508.219.30.22969
45.8582937.50.423981611.42664214.612.25.8582915.60.412721
56.55119754.20.52906887.1012119.17.66.551197.57.90.505848
66.85128358.10.190593144.1578955.34.56.85128313.60.172004
77.35147066.50.311532136.2133737.96.77.351468.212.70.276922
88.15177780.40.12526173.6581494.73.98.151777170.121346
98.45188585.30.25411125.26216.75.68.451885.2120.246673
108.65195488.50.233553103.9683435.14.38.6519549.80.227502
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3218198.70.588247109.65115912.310.39.32179.910.20.532155
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 10
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 11
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5401.80.178291175.0357634.33.63.542.417.20.165139
24.32831914.30.7447381721.52343618.415.44.32832416.80.721337
34.5539417.70.6418871415.1916291310.94.55394.114.30.601282
44.78645920.60.07263670.892780.80.64.786459.36.70.075216
54.92449722.30.093598121.9263671.71.44.924495.8120.090221
65.11655024.70.122054132.5838992.21.85.116550.213.40.108604
75.257325.70.215687133.0863742.62.25.2571.212.60.138224
85.22558426.20.523941108.15077775.85.225584.99.50.482433
95.31762528.10.22514572.8272912.425.317625.16.90.229776
105.41767030.10.29629773.9292023.42.85.417669.27.30.302012
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.47869731.30.01074850.0912050.10.15.478696.55.50.009437
125.6175633.90.35413185.0016794.33.65.61755.780.354446
135.64877334.70.00155371.1084680.90.85.6487836.90.091295
145.779635.70.4236897.1130176.15.15.7795.39.10.43923
155.78583437.40.144198102.37130721.75.7858349.60.139495
165.8586338.70.065242182.4937852.11.85.85875.817.70.079459
176.141100545.10.19405451.6678991.41.26.1411002.94.60.203354
186.368111650.10.202575103.54076232.56.3681114.79.70.205155
196.474116852.40.148179122.9733052.52.16.4741168.7120.139268
206.55120554.10.04311650.3323240.30.26.551203.84.90.038176
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.85128457.60.191105123.6142843.12.66.851284.911.90.172055
227.35146465.70.314563147.0794936.15.17.351463.813.90.288388
238.15176879.40.062696202.430812.11.78.151767.819.50.070228
248.45189284.90.120808122.5122582.21.88.451893.412.10.11751
258.65196888.30.11600591.7731681.51.38.651967.49.10.109452
269.3220498.90.441357117.4461416.45.39.32202.810.60.394912
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 11
LANE TYPE: Specimen
ACTUAL GEL LANE: 12
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.19325411.60.772661519.71926719.116.74.193257.614.70.754987
24.432214.60.5105481210.2464859.98.74.4321.611.80.491621
34.561374170.057873121.2164611.214.561379.912.30.055894
44.72642019.10.027271110.569960.60.54.726417.611.50.028004
54.89946821.30.103001142.309982.224.899462.813.80.094279
64.98649222.40.404773149.9996829.78.54.986493.813.60.413978
75.1353224.20.084883142.1568722.11.85.13535.313.80.088343
85.27658626.70.16929123.4460353.32.95.276586.611.60.167291
95.4667030.50.297745249.146548.97.85.46672.324.40.21109
105.51769631.70.605175221.985251.91.75.517698.422.40.049932
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.54871032.30.6226133030.77756929.826.15.54871230.40.570413
125.76580936.80.056266121.2236411.215.765809.512.40.055682
136.07195443.40.156094102.9183352.82.56.071954.210.10.163101
146.255104347.50.046439121.0851391.10.96.2551043.212.30.049915
156.311107048.70.32063984.9073544.84.26.3111070.88.40.329531
166.41111850.90.072376131.5969751.51.46.411119.712.60.071369
PeakCounter: 16
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 12
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5542.40.276589209.54203412.910.93.557.220.50.262905
24.5538817.40.2722162210.18534313.711.74.55383.221.90.262315
35.257025.60.152326246.7324479.17.75.256524.30.156371
45.8587239.10.4936731512.35407516.614.15.85873.215.10.461514
56.55120654.10.366596106.1406538.376.5512059.70.356644
66.85129057.90.179857144.114945.54.76.851290.514.10.164315
77.35147266.10.245918155.96638986.87.351471.615.30.220271
88.15178179.90.11845173.4403864.63.98.151780.816.70.116334
98.451894850.214174114.0413325.44.68.451894.911.10.206323
108.65196688.20.182326103.02914.13.58.651965.69.50.179247
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3219898.70.433159138.65811111.79.99.32198.212.60.389128
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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pI 9.30 label for gel schematic
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pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 92
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 8
LANE TYPE: Specimen
ACTUAL GEL LANE: 9
PeakCount: 17 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 9
LANE TYPE: pI Markers
ACTUAL GEL LANE: 10
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 10
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 11
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 11
LANE TYPE: Specimen
ACTUAL GEL LANE: 12
PeakCount: 16 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 12
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
PeakCount: 11 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

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Last updated on 2003-FEB-27 by bjt/frf/nln