Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Pink Salmon  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Oncorhynchus gorbuscha
FDA Seafood List Common Name: Pink Salmon
FDA Seafood List Market Name: Salmon
Unique IEF Gel Code: 19981215gel04
Unique Web Code: pkh01Srr
Tissue Aliquot Code: oncogorb H94-004 #10m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55172.20.085606230.4522737.254.5516.730.0849327
25.2108140.070850950.5270658.45.95.2108.64.50.0658305
35.8521728.20.12796630.64948810.47.25.85217.230.122842
46.5535546.10.11519671.2808120.414.36.55353.56.70.108475
56.8539150.80.027597350.2107513.42.36.85390.64.90.0242793
67.3545959.60.058295930.3295085.33.77.35458.53.30.0566259
78.1558075.30.10533361.0216316.311.48.15579.75.60.103414
88.4564483.60.11341761.2115219.313.58.45644.26.30.108215
98.65701910.087415440.5802089.36.58.65701.23.90.0838079
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.676334.30.061468230.3006520.30.24.67632.72.70.0621927
25.00377100.18010161.636731.41.25.00376.86.20.148718
35.36713217.10.11129750.9228070.80.75.367132.85.40.0962418
45.543162210.41676163.722273.12.75.543161.76.20.340739
56.10626634.52.3328427.2565665.36.106266.11.62.55458
66.19528436.90.60163421.900071.61.46.195283.61.80.588794
76.2729938.80.25991341.366681.116.27299.43.60.217015
86.37432041.62.31313311.94479.98.86.374321.72.62.60753
96.47334044.22.2707728.043976.75.96.473339.81.82.5193
106.51834945.30.075385430.3314690.30.26.518348.82.60.0709654
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.62536447.30.28227895.230284.43.86.625370.19.20.320383
126.70437348.41.6587613.123422.62.36.704372.51.31.31504
136.75737949.21.9403327.128325.95.26.757379.91.72.36274
146.86839250.92.2240215.047264.23.76.868392.31.22.39885
157.08742254.80.0491605100.726760.60.57.087426.39.50.0430541
167.1643256.10.60963721.715731.41.37.16431.61.60.619612
177.29945158.62.3006211.25939.48.37.299452.42.42.62396
187.49548062.32.30444313.837511.510.27.495479.932.60465
197.76552167.71.8816413.282511.19.87.765520.941.87474
207.89754170.30.22400762.232451.91.67.897541.85.90.212775
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.73272193.61.3302537.2426865.38.732720.13.21.27253
228.76473094.80.70671466.092835.14.58.7647325.90.583165
238.8274696.90.5868565.773144.84.28.82745.35.90.554926
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55151.90.069656140.477876.34.54.5515.340.0679905
25.29912.80.051776970.6325088.35.95.299.56.80.0521892
35.8521227.40.10624540.85642511.385.85211.84.50.107474
46.55356460.14933371.6980122.315.96.5535570.136404
56.8538850.10.035752450.2916593.82.76.85388.45.10.0320669
67.35457590.073011730.4117145.43.97.35457.53.40.0692242
78.1557974.80.10999161.1117714.610.48.15579.160.104395
88.4563782.30.12205771.3655117.912.88.45636.86.60.116585
98.6569489.70.11540640.762085107.18.65693.43.90.110601
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55212.70.048797130.2353750.20.24.5520.92.70.0491066
24.6253140.043878830.2063340.20.24.62531.32.60.0455499
34.849617.90.033371360.3747470.40.34.84961.66.20.0340472
44.961769.80.16139481.890031.81.74.96176.98.10.131751
55.2108140.061767580.7492020.70.75.2107.18.10.0523736
65.35513417.30.056790260.5328430.50.55.355134.26.20.0484447
75.52216220.90.29395762.939182.72.65.522161.26.50.256686
85.85217280.067470930.4227620.40.45.85217.23.50.068209
96.06926233.91.188324.464914.23.96.0692612.31.10351
106.16628236.40.12557551.0090.90.96.166281.24.80.119136
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.25830138.90.097534360.8430710.80.76.258299.85.50.0857667
126.38532742.21.832539.706329.18.66.385326.731.79887
136.48234744.81.5905436.602336.25.86.482346.42.91.28942
146.526356460.073584320.3112690.30.36.526356.22.30.0758524
156.66537348.20.19081230.7468730.70.76.665371.23.50.122093
166.722379490.37689853.51343.33.16.722379.15.30.371735
176.80938850.11.2748736.896386.46.16.809387.13.11.2509
186.88439751.30.75850734.176853.93.76.884397.63.40.689982
197.1543456.10.25623941.783421.71.67.15434.44.30.232871
207.30945658.92.3408413.320512.411.87.3094563.62.08292
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.4848162.12.33206314.745813.8137.48482.33.32.54568
227.76152367.61.1879947.181846.76.37.761523.13.71.10926
237.90254470.30.19344192.642932.52.37.902546.68.90.168406
248.1558175.10.082442550.7367410.70.78.15581.25.20.0806233
258.45650840.080837950.6996010.70.68.45649.750.0785935
268.65704910.372891510.29459.69.18.6570915.10.384762
278.70671992.90.6120931.924671.81.78.7067193.20.342172
288.7573194.40.53637654.085073.83.68.75731.150.46556
298.79174295.90.47997154.028273.83.68.7917425.10.446516
PeakCounter: 29
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.625374.80.05429520.2349320.20.24.62536.82.40.0552436
24.9498210.50.17074961.506531.41.34.94982.16.20.136137
35.33613917.90.07625340.5407560.50.55.3361404.10.0752986
45.51416821.60.33682162.93432.82.55.514167.46.40.259239
56.05126433.92.0775726.3265665.46.051263.91.62.28821
66.15328536.60.19595631.0959310.96.153284.23.40.184511
76.2430338.90.17012130.8136080.80.76.24302.72.90.160408
86.356327422.3454229.6679.28.26.356327.722.69998
96.45834844.72.3060815.463265.24.66.458347.51.32.44271
106.52136146.40.0440803120.6922470.70.66.521356120.0325471
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.62837548.20.22963310.3690490.40.36.628372.11.50.14041
126.687381490.47927964.1632143.56.687380.36.10.382975
136.75538849.92.2661215.096414.84.36.755388.41.12.61708
146.86740051.42.242913.888083.73.36.867400.20.92.33345
157.13243756.20.4780621.586181.51.37.132437.31.80.485191
167.28345858.92.31715311.865911.310.17.283457.82.52.63629
177.46648562.32.31984416.293615.513.87.466484.63.52.60795
187.74752867.91.93707311.506410.99.87.747527.83.51.87854
197.87254770.30.15956641.185311.117.872546.64.20.159436
208.70972693.31.74739.365688.97.98.709725.93.21.63996
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.74573694.60.61368554.353894.13.78.745735.94.70.520686
228.79675096.40.63626766.306965.38.796748.16.20.576594
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55303.80.076584440.4968835.23.74.5530.43.70.0760643
25.212415.90.094233750.7987978.365.2124.15.10.0878622
35.85226290.10063650.8594968.96.55.85225.84.80.101893
46.5537147.60.21006561.9103119.814.46.55370.75.60.191634
56.8540151.40.05148350.4075034.23.16.85401.35.20.0446413
67.3547160.40.075269140.4565494.73.47.35471.13.60.0719534
78.1559676.40.097055491.3862614.410.58.15595.78.80.0891656
88.4565684.10.14510692.3052423.917.48.45655.49.40.138399
98.6571391.40.15572541.0209510.67.78.65712.73.90.148043
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 101
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 29 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln