RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ruvettus(*) pretiosus(*)
FDA Seafood List Common Name: Oilfish(*)
FDA Seafood List Market Name: Oilfish(*)
Unique IEF Gel Code: 19950830gel14
Unique Web Code: oig02Srr
Tissue Aliquot Code: ruvepret H95-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	26	2.3	0.154778	13	4.23118	18.9	14.4	3.5	21.9	13.4	0.17818
2	4.55	185	16.2	0.209598	4	1.35529	6	4.6	4.55	185.4	3.9	0.194169
3	5.2	272	23.8	0.229661	4	1.51004	6.7	5.1	5.2	272.1	4	0.214941
4	5.85	427	37.3	0.38582	3	2.22769	9.9	7.6	5.85	426.6	3.2	0.393738
5	6.55	608	53.1	0.196007	5	1.68331	7.5	5.7	6.55	606.3	5.3	0.178943
6	6.85	651	56.9	0.108114	9	1.41941	6.3	4.8	6.85	651.5	8.7	0.0922988
7	7.35	740	64.7	0.300049	6	2.77075	12.4	9.4	7.35	739.2	5.7	0.274501
8	8.15	893	78.1	0.0309626	10	0.563941	2.5	1.9	8.15	893.1	9.8	0.0324355
9	8.45	955	83.5	0.0982808	9	1.36334	6.1	4.6	8.45	954.7	8.8	0.0877516
10	8.65	992	86.7	0.0905132	7	1.02254	4.6	3.5	8.65	992	6.6	0.0874331
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1120	97.9	0.508256	5	4.27475	19.1	14.5	9.3	1118.7	5.2	0.46198

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	21	1.8	0.152541	8	1.90219	4.3	3.6	3.5	20.3	7.7	0.139659
2	3.62	39	3.4	0.232905	7	2.77322	6.3	5.3	3.62	38	7.3	0.214095
3	4.55	178	15.6	0.335336	5	2.94298	6.7	5.6	4.55	178.5	4.8	0.34306
4	4.678	199	17.4	0.125695	6	1.2836	2.9	2.4	4.678	200.2	5.6	0.12829
5	4.781	216	18.9	0.055344	8	0.808045	1.8	1.5	4.781	214.7	8.2	0.0559134
6	4.884	233	20.4	0.021809	7	0.301014	0.7	0.6	4.884	231.9	7	0.0242371
7	5.2	285	24.9	0.172127	11	3.04084	6.9	5.8	5.2	285.5	11.1	0.154992
8	5.278	304	26.6	0.0477525	7	0.654376	1.5	1.2	5.278	307.9	7.1	0.0523347
9	5.686	404	35.3	0.194466	6	1.90908	4.4	3.6	5.686	404.1	6	0.179786
10	5.764	423	37	0.114003	7	1.26568	2.9	2.4	5.764	422.1	6.8	0.104366
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	444	38.8	0.542847	4	4.03832	9.2	7.7	5.85	444.8	4.2	0.547537
12	5.967	471	41.2	0.288488	4	1.94653	4.4	3.7	5.967	471.2	4.2	0.261042
13	6.062	493	43.1	0.145607	3	0.751558	1.7	1.4	6.062	492.8	3.2	0.133612
14	6.114	505	44.1	0.0775175	7	0.809714	1.9	1.5	6.114	502.4	7.2	0.0634703
15	6.161	516	45.1	0.0112198	3	0.0730356	0.2	0.1	6.161	519.3	3.1	0.0134718
16	6.317	552	48.3	0.0393602	5	0.290148	0.7	0.6	6.317	551.5	4.7	0.0351851
17	6.468	587	51.3	0.0509642	2	0.0981299	0.2	0.2	6.468	586.2	2.4	0.0232866
18	6.494	593	51.8	0.0756363	8	0.790187	1.8	1.5	6.494	593.9	7.8	0.0573905
19	6.55	606	53	0.129567	4	0.777785	1.8	1.5	6.55	605.3	3.5	0.12381
20	6.7	626	54.7	0.0788912	3	0.4375	1	0.8	6.7	625.5	3.5	0.0714951
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.79	638	55.8	0.278932	2	0.842876	1.9	1.6	6.79	638	1.8	0.257779
22	6.85	646	56.5	0.197389	5	1.77575	4.1	3.4	6.85	647.5	5.4	0.186006
23	6.95	665	58.1	0.422711	2	1.66829	3.8	3.2	6.95	665.1	2.3	0.408148
24	7.05	684	59.8	0.232126	4	1.45689	3.3	2.8	7.05	683.3	3.7	0.224907
25	7.171	707	61.8	0.0466118	18	1.86274	4.3	3.5	7.171	715	18	0.0583865
26	7.35	741	64.8	0.275445	5	2.12128	4.8	4	7.35	740.7	4.6	0.258232
27	8.15	887	77.5	0.00695515	6	0.146359	0.3	0.3	8.15	883.1	6.4	0.0129734
28	8.45	957	83.7	0.0877926	8	1.14008	2.6	2.2	8.45	956.9	8	0.0799624
29	8.65	995	87	0.0875136	7	1.02436	2.3	2	8.65	994.1	7.2	0.0801231
30	9.3	1122	98.1	0.507512	6	4.83578	11	9.2	9.3	1120.5	5.9	0.463623

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.557	39	3.4	0.16252	4	1.17444	4.8	3.7	3.557	39	4.1	0.16284
2	4.463	174	15.2	0.367236	5	2.9781	12.2	9.5	4.463	174.2	4.5	0.372038
3	4.688	207	18.1	0.0417484	8	0.535472	2.2	1.7	4.688	208.3	7.6	0.039785
4	4.839	229	20	0.014391	5	0.138312	0.6	0.4	4.839	228.4	4.8	0.0161973
5	5.121	270	23.6	0.039414	9	0.604183	2.5	1.9	5.121	272.1	8.6	0.0394213
6	5.531	363	31.7	0.0203261	4	0.127286	0.5	0.4	5.531	362	3.8	0.0190396
7	5.657	394	34.4	0.204321	6	2.04463	8.4	6.5	5.657	393.4	5.6	0.204921
8	5.742	415	36.2	0.106625	7	1.29936	5.3	4.1	5.742	413.1	7.3	0.100741
9	5.824	435	38	0.68177	3	3.30893	13.5	10.6	5.824	435.2	2.8	0.665083
10	5.929	460	40.2	0.285353	4	1.80828	7.4	5.8	5.929	459.3	4.2	0.243923
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.019	481	42	0.156748	3	0.833752	3.4	2.7	6.019	481.4	3	0.156346
12	6.066	492	43	0.122374	5	1.01493	4.1	3.2	6.066	492.7	5.1	0.11144
13	6.139	509	44.5	0.016495	2	0.089777	0.4	0.3	6.139	508	2.4	0.0208425
14	6.267	539	47.1	0.0205759	4	0.19445	0.8	0.6	6.267	540.7	3.7	0.0299498
15	6.426	576	50.3	0.0818981	3	0.435227	1.8	1.4	6.426	575.7	3.3	0.0753908
16	6.464	585	51.1	0.0616801	4	0.438529	1.8	1.4	6.464	583.6	3.5	0.0703231
17	6.605	613	53.5	0.110277	3	0.549756	2.2	1.8	6.605	612.5	2.9	0.107764
18	6.68	624	54.5	0.276362	3	1.13131	4.6	3.6	6.68	624	2.5	0.253843
19	6.741	633	55.3	0.208334	5	1.69012	6.9	5.4	6.741	633.5	4.9	0.193972
20	6.866	652	56.9	0.372011	3	1.84586	7.5	5.9	6.866	652.8	2.9	0.359621
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.968	671	58.6	0.259702	4	1.78862	7.3	5.7	6.968	670.9	4	0.252567
22	7.069	690	60.3	0.0399703	6	0.452841	1.8	1.4	7.069	692.2	5.9	0.0433702

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.5	0.183153	11	3.26491	14.9	10.3	3.5	41.6	10.7	0.172588
2	4.55	196	17.1	0.199275	5	1.61214	7.3	5.1	4.55	195.4	4.8	0.191021
3	5.2	278	24.3	0.198781	5	1.62875	7.4	5.1	5.2	277.6	4.9	0.189148
4	5.85	439	38.4	0.410507	3	2.62655	12	8.3	5.85	439.2	3.5	0.424615
5	6.55	604	52.8	0.232133	6	2.25301	10.3	7.1	6.55	603.3	5.9	0.214061
6	6.85	652	57	0.13133	8	1.62629	7.4	5.1	6.85	651.9	8.2	0.111885
7	7.35	744	65	0.268957	6	2.46501	11.2	7.8	7.35	743.3	5.6	0.246532
8	8.15	900	78.7	0.0272169	10	0.520343	2.4	1.6	8.15	900.2	9.7	0.0302996
9	8.45	957	83.7	0.086678	7	1.01096	4.6	3.2	8.45	957.3	6.9	0.082648
10	8.65	998	87.2	0.0832092	6	0.838741	3.8	2.6	8.65	995.2	6	0.0786187
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1121	98	0.46697	6	4.12469	18.8	13	9.3	1119.8	5.5	0.420517

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.527	43	3.8	0.250102	4	1.73887	6.5	4.8	3.527	43.4	3.9	0.252413
2	4.466	183	16	0.451899	4	3.15021	11.8	8.6	4.466	182.6	3.8	0.464994
3	4.756	222	19.4	0.060483	5	0.585454	2.2	1.6	4.756	219.5	4.9	0.0673533
4	4.904	241	21	0.0314684	4	0.247133	0.9	0.7	4.904	239.5	4.3	0.0325687
5	5.224	285	24.9	0.0502579	12	0.997172	3.7	2.7	5.224	286.4	11.6	0.0485353
6	5.567	370	32.3	0.0158352	3	0.112026	0.4	0.3	5.567	371.8	3.5	0.0181877
7	5.701	403	35.2	0.227001	6	2.24177	8.4	6.1	5.701	402.7	6	0.211001
8	5.785	424	37	0.100769	7	1.29304	4.9	3.5	5.785	421.8	7.3	0.0995173
9	5.862	443	38.7	0.616359	3	3.36264	12.6	9.2	5.862	442.9	3.2	0.600817
10	5.958	466	40.7	0.275643	7	2.69244	10.1	7.4	5.958	466	6.8	0.223125
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.046	487	42.5	0.16365	4	1.12912	4.2	3.1	6.046	487.1	4.2	0.151122
12	6.088	497	43.4	0.125158	5	1.15241	4.3	3.2	6.088	498.2	5.3	0.121717
13	6.15	512	44.7	0.0169005	2	0.0678415	0.3	0.2	6.15	513.3	1.8	0.021068
14	6.292	546	47.7	0.0334934	5	0.303644	1.1	0.8	6.292	544.8	4.6	0.0374885
15	6.433	580	50.7	0.0608836	4	0.365728	1.4	1	6.433	580.2	3.5	0.0585267
16	6.463	587	51.3	0.0616204	3	0.366433	1.4	1	6.463	587.4	3.4	0.0614534
17	6.607	617	53.9	0.0876191	3	0.438561	1.6	1.2	6.607	616.7	2.9	0.0861859
18	6.683	629	54.9	0.231941	3	0.979431	3.7	2.7	6.683	628.5	2.5	0.220414
19	6.739	638	55.7	0.210416	4	1.58871	6	4.3	6.739	637.8	4.3	0.208258
20	6.847	655	57.2	0.29747	3	1.77304	6.7	4.9	6.847	655.8	3.5	0.289494
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.957	675	59	0.248839	4	1.60185	6	4.4	6.957	675	3.7	0.244993
22	7.067	695	60.7	0.0490548	5	0.445602	1.7	1.2	7.067	696.2	5.4	0.0469054

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	3.3	0.268752	15	6.75238	25.4	20	3.5	36.3	14.8	0.256923
2	4.55	195	17	0.235844	4	1.6452	6.2	4.9	4.55	195.5	4.3	0.217364
3	5.2	280	24.4	0.186094	5	1.57819	5.9	4.7	5.2	280.5	5	0.177729
4	5.85	441	38.4	0.362109	4	2.42227	9.1	7.2	5.85	440.6	3.7	0.368841
5	6.55	612	53.4	0.175527	7	1.93212	7.3	5.7	6.55	610.7	6.6	0.16628
6	6.85	659	57.5	0.0973764	9	1.36308	5.1	4	6.85	659	9	0.0852022
7	7.35	749	65.3	0.228711	7	2.65427	10	7.9	7.35	748	7.1	0.212055
8	8.15	905	78.9	0.0409525	11	0.774092	2.9	2.3	8.15	905	11.4	0.0382194
9	8.45	961	83.8	0.102299	8	1.35814	5.1	4	8.45	961.3	8.1	0.0944808
10	8.65	1002	87.4	0.0789505	6	0.800182	3	2.4	8.65	1000.9	5.9	0.0771325
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1125	98.1	0.545749	6	5.27652	19.9	15.7	9.3	1123	6.1	0.4873

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 107

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 18.900 4.550 6.000 5.200 6.700 5.850 9.900 6.550 7.500 6.850 6.300 7.350 12.400 8.150 2.500 8.450 6.100 8.650 4.600 9.300 19.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 4.300 3.620 6.300 4.550 6.700 4.678 2.900 4.781 1.800 4.884 0.700 5.200 6.900 5.278 1.500 5.686 4.400 5.764 2.900 5.850 9.200 5.967 4.400 6.062 1.700 6.114 1.900 6.161 0.200 6.317 0.700 6.468 0.200 6.494 1.800 6.550 1.800 6.700 1.000 6.790 1.900 6.850 4.100 6.950 3.800 7.050 3.300 7.171 4.300 7.350 4.800 8.150 0.300 8.450 2.600 8.650 2.300 9.300 11.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 30 Return to Lane Navigator
3.557 4.800 4.463 12.200 4.688 2.200 4.839 0.600 5.121 2.500 5.531 0.500 5.657 8.400 5.742 5.300 5.824 13.500 5.929 7.400 6.019 3.400 6.066 4.100 6.139 0.400 6.267 0.800 6.426 1.800 6.464 1.800 6.605 2.200 6.680 4.600 6.741 6.900 6.866 7.500 6.968 7.300 7.069 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 22 Return to Lane Navigator
3.500 14.900 4.550 7.300 5.200 7.400 5.850 12.000 6.550 10.300 6.850 7.400 7.350 11.200 8.150 2.400 8.450 4.600 8.650 3.800 9.300 18.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.527 6.500 4.466 11.800 4.756 2.200 4.904 0.900 5.224 3.700 5.567 0.400 5.701 8.400 5.785 4.900 5.862 12.600 5.958 10.100 6.046 4.200 6.088 4.300 6.150 0.300 6.292 1.100 6.433 1.400 6.463 1.400 6.607 1.600 6.683 3.700 6.739 6.000 6.847 6.700 6.957 6.000 7.067 1.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 22 Return to Lane Navigator
3.500 25.400 4.550 6.200 5.200 5.900 5.850 9.100 6.550 7.300 6.850 5.100 7.350 10.000 8.150 2.900 8.450 5.100 8.650 3.000 9.300 19.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Oilfish(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.