RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Chinook Salmon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Oncorhynchus tshawytsha
FDA Seafood List Common Name: Chinook Salmon
FDA Seafood List Market Name: Salmon, Chinook (King, Spring)
Unique IEF Gel Code: 19960308gel03
Unique Web Code: kgh01Srr
Tissue Aliquot Code: oncotsha I94-001 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	25	3.3	0.0860518	4	0.528186	5.7	4.3	4.55	25.7	3.6	0.083832
2	5.2	116	15.3	0.100945	4	0.66942	7.2	5.4	5.2	115.7	3.8	0.100523
3	5.85	208	27.5	0.159938	4	1.00575	10.8	8.1	5.85	207.9	3.6	0.158339
4	6.55	338	44.7	0.0613225	6	0.614432	6.6	5	6.55	336.9	5.9	0.0590586
5	6.85	373	49.3	0.0625214	5	0.504473	5.4	4.1	6.85	372.7	5.1	0.0562285
6	7.35	435	57.5	0.131305	4	0.918865	9.8	7.4	7.35	435.2	4.1	0.127772
7	8.15	555	73.4	0.140456	6	1.47789	15.8	12	8.15	554.8	6.1	0.13734
8	8.45	607	80.3	0.112555	10	2.02786	21.7	16.4	8.45	607.1	10.2	0.111798
9	8.65	666	88.1	0.102315	5	0.869584	9.3	7	8.65	665.9	4.9	0.0993198
10	9.3	737	97.5	0.0893174	5	0.722873	7.7	5.8	9.3	735.9	4.6	0.0881015

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.646	37	4.9	0.0516576	3	0.273184	0.8	0.7	4.646	37.3	2.9	0.0522635
2	4.809	59	7.8	0.124774	7	1.28391	3.7	3.4	4.809	58.9	6.5	0.110743
3	4.934	76	10.1	0.0741193	8	1.04964	3	2.7	4.934	74.9	8	0.0741603
4	5.111	100	13.2	0.0249277	15	0.80936	2.3	2.1	5.111	114.9	15.4	0.0295804
5	5.342	132	17.5	0.155619	7	1.53269	4.4	4	5.342	130.5	7.3	0.118379
6	5.42	143	18.9	0.0317929	3	0.146801	0.4	0.4	5.42	143	3	0.0274099
7	5.534	159	21.1	0.133112	2	0.523454	1.5	1.4	5.534	158.8	2.4	0.122285
8	5.676	179	23.7	0.0480168	4	0.295995	0.9	0.8	5.676	178.6	3.5	0.0476461
9	5.74	188	24.9	0.0526956	5	0.428923	1.2	1.1	5.74	188.5	4.9	0.0493909
10	5.947	222	29.4	0.203721	3	1.06325	3.1	2.8	5.947	222.8	3.1	0.196042
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.026	237	31.4	0.193603	3	1.13051	3.2	3	6.026	237.4	3.4	0.185945
12	6.089	249	33	0.0653488	5	0.546106	1.6	1.4	6.089	249.1	4.7	0.0652142
13	6.214	273	36.2	0.287829	3	1.24463	3.6	3.3	6.214	273.1	2.5	0.278303
14	6.309	291	38.5	0.16924	3	0.873704	2.5	2.3	6.309	290.3	3	0.165945
15	6.335	296	39.2	0.120036	2	0.494445	1.4	1.3	6.335	295.7	2.4	0.116458
16	6.403	309	40.9	0.0906765	2	0.34192	1	0.9	6.403	308.6	2.1	0.0907657
17	6.503	328	43.4	0.527828	3	2.44003	7	6.4	6.503	327.6	2.6	0.537663
18	6.674	351	46.5	0.242004	5	2.20243	6.3	5.8	6.674	351.2	5.4	0.228793
19	6.771	362	47.9	0.13853	2	0.460713	1.3	1.2	6.771	361.3	1.7	0.156344
20	6.806	366	48.5	0.179549	2	0.715466	2.1	1.9	6.806	366.3	2.2	0.186073
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.875	374	49.5	0.167041	2	0.487199	1.4	1.3	6.875	374.1	1.6	0.16857
22	6.989	388	51.4	0.0582221	5	0.415659	1.2	1.1	6.989	388.8	4.7	0.0497812
23	7.08	399	52.8	0.385916	2	1.63352	4.7	4.3	7.08	399.2	2.4	0.379179
24	7.26	421	55.8	0.501264	3	2.93954	8.4	7.7	7.26	420.7	3.2	0.516351
25	7.377	436	57.7	0.206208	3	0.913043	2.6	2.4	7.377	436	2.6	0.196556
26	7.47	450	59.6	0.492902	3	2.58257	7.4	6.8	7.47	449.7	3	0.488394
27	7.723	488	64.6	0.210716	3	1.23681	3.6	3.2	7.723	488.3	3.3	0.212087
28	7.87	510	67.5	0.193048	5	1.58927	4.6	4.2	7.87	510.4	4.7	0.190907
29	8.613	653	86.5	0.179718	10	2.87396	8.3	7.5	8.613	652.6	9.6	0.168595
30	8.746	674	89.3	0.0816792	9	1.32	3.8	3.5	8.746	673.5	9.3	0.0801763
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.067	709	93.9	0.0850004	6	0.953584	2.7	2.5	9.067	709.2	6.3	0.0858832

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.2	0.0691897	4	0.435472	4.3	3	4.55	23.8	3.6	0.0679927
2	5.2	109	14.4	0.0962982	4	0.600757	5.9	4.1	5.2	109	3.6	0.0945659
3	5.85	200	26.5	0.152153	4	0.993963	9.7	6.8	5.85	199.6	3.7	0.151505
4	6.55	337	44.6	0.082162	8	1.0323	10.1	7.1	6.55	335	7.8	0.0747605
5	6.85	370	49	0.0548809	6	0.516195	5.1	3.5	6.85	370.4	5.9	0.0493163
6	7.35	430	57	0.120075	4	0.792446	7.8	5.4	7.35	429.9	3.9	0.115918
7	8.15	550	72.8	0.164111	6	1.81057	17.7	12.5	8.15	549.7	6.5	0.157622
8	8.45	606	80.3	0.132409	9	2.20153	21.6	15.1	8.45	604.5	9.4	0.132187
9	8.65	662	87.7	0.120716	5	0.985095	9.6	6.8	8.65	661.1	4.7	0.117842
10	9.3	733	97.1	0.114929	4	0.845835	8.3	5.8	9.3	732.3	4.2	0.113372

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	22	2.9	0.0701179	3	0.396407	1.7	1.6	4.55	22.1	3.1	0.0712678
2	4.646	35	4.6	0.0146065	2	0.0647265	0.3	0.3	4.646	35	2.5	0.0148539
3	4.772	52	6.9	0.0314379	6	0.346745	1.5	1.4	4.772	53.3	6.4	0.0306557
4	4.941	75	9.9	0.0694731	11	1.27385	5.4	5.1	4.941	72.7	11.3	0.0635144
5	5.2	110	14.6	0.0627204	6	0.585196	2.5	2.3	5.2	109.5	5.9	0.0561316
6	5.349	130	17.2	0.0474911	6	0.413072	1.7	1.6	5.349	128.4	5.6	0.0415891
7	5.544	156	20.7	0.0491923	3	0.234039	1	0.9	5.544	155.4	2.8	0.0465937
8	5.671	173	22.9	0.0188923	7	0.256314	1.1	1	5.671	173.4	7.4	0.0194381
9	5.85	197	26.1	0.106054	3	0.643002	2.7	2.6	5.85	196.6	3.4	0.108219
10	5.933	213	28.2	0.0478227	4	0.312955	1.3	1.2	5.933	213.4	4	0.0443914
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.017	229	30.4	0.045568	6	0.440498	1.9	1.8	6.017	228.5	6	0.0413365
12	6.09	243	32.2	0.0109752	5	0.103476	0.4	0.4	6.09	243.7	5.4	0.0108873
13	6.251	274	36.3	0.120607	2	0.466024	2	1.9	6.251	273.9	2.2	0.120504
14	6.34	291	38.6	0.0914083	4	0.666509	2.8	2.7	6.34	292	4.4	0.0851232
15	6.434	309	41	0.0347501	3	0.148632	0.6	0.6	6.434	308.7	2.6	0.0316587
16	6.538	329	43.6	0.384422	3	1.78012	7.5	7.1	6.538	328.7	2.7	0.375924
17	6.745	355	47.1	0.197773	5	1.63402	6.9	6.5	6.745	354.6	5	0.182911
18	6.828	365	48.4	0.113277	2	0.292849	1.2	1.2	6.828	363.8	1.9	0.0879647
19	6.86	369	48.9	0.212008	3	1.17278	5	4.7	6.86	369	3.2	0.209944
20	6.915	376	49.9	0.0883249	3	0.409931	1.7	1.6	6.915	375.7	2.6	0.0875262
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.094	399	52.9	0.231673	3	1.05291	4.4	4.2	7.094	399	2.7	0.218015
22	7.241	418	55.4	0.3755	3	2.06816	8.7	8.2	7.241	418.5	3	0.38546
23	7.35	432	57.3	0.122882	4	0.936818	4	3.7	7.35	430.5	4.3	0.123309
24	7.442	445	59	0.313469	3	1.70811	7.2	6.8	7.442	444.6	3.1	0.314926
25	7.69	480	63.7	0.105794	3	0.59344	2.5	2.4	7.69	479.9	3.2	0.105841
26	7.888	508	67.4	0.0717647	5	0.639556	2.7	2.6	7.888	508.6	5.3	0.0687289
27	8.15	545	72.3	0.0841263	6	0.841318	3.6	3.4	8.15	544.6	5.9	0.0809663
28	8.45	601	79.7	0.0884768	7	1.02345	4.3	4.1	8.45	600.6	6.7	0.0858135
29	8.607	648	85.9	0.0728341	7	0.952581	4	3.8	8.607	647	7.1	0.0755
30	8.65	661	87.7	0.0849738	5	0.650216	2.7	2.6	8.65	661.6	4.6	0.0800887
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.056	706	93.6	0.0469314	12	1.05932	4.5	4.2	9.056	708.5	12.3	0.0484732
32	9.3	733	97.2	0.0852495	4	0.51266	2.2	2	9.3	732.5	3.6	0.0802275

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.666	37	4.9	0.0472128	3	0.220852	0.6	0.6	4.666	37.2	2.6	0.0475303
2	4.832	60	8	0.122604	7	1.31317	3.7	3.5	4.832	59.6	6.9	0.107521
3	4.969	79	10.5	0.0735585	8	0.960184	2.7	2.5	4.969	77.6	7.7	0.0707034
4	5.135	102	13.5	0.0296669	18	1.13997	3.2	3	5.135	128.6	18	0.0357316
5	5.36	133	17.6	0.176225	7	1.53501	4.4	4.1	5.36	133.3	7.4	0.117028
6	5.549	159	21.1	0.106874	5	0.761017	2.2	2	5.549	158.4	4.6	0.0936775
7	5.679	177	23.5	0.0352799	4	0.255442	0.7	0.7	5.679	175.6	4.1	0.0347803
8	5.73	184	24.4	0.0369603	5	0.301863	0.9	0.8	5.73	184.4	4.7	0.0364481
9	5.958	220	29.2	0.131205	4	1.02896	2.9	2.7	5.958	220.3	4.4	0.133097
10	6.047	236	31.3	0.115492	5	1.05584	3	2.8	6.047	235.9	5.2	0.114446
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.125	250	33.2	0.0431994	5	0.409138	1.2	1.1	6.125	249	5.1	0.0450476
12	6.252	273	36.2	0.274901	3	1.559	4.4	4.1	6.252	273.4	3.4	0.258343
13	6.341	289	38.3	0.214078	6	2.21631	6.3	5.9	6.341	289.3	6.2	0.200448
14	6.424	304	40.3	0.0836571	3	0.432469	1.2	1.1	6.424	304.1	3.1	0.0794584
15	6.524	322	42.7	0.468416	3	2.44034	6.9	6.5	6.524	321.9	2.9	0.469258
16	6.726	350	46.4	0.170945	6	1.6708	4.8	4.4	6.726	349.3	5.9	0.159707
17	6.81	361	47.9	0.113113	1	0.220427	0.6	0.6	6.81	361.1	1.5	0.083682
18	6.855	367	48.7	0.166139	4	1.08572	3.1	2.9	6.855	367.2	3.6	0.171528
19	6.918	375	49.7	0.160512	3	0.732264	2.1	1.9	6.918	375.2	2.6	0.160766
20	7.028	389	51.6	0.0456767	4	0.304739	0.9	0.8	7.028	389.4	3.6	0.0475135
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.099	398	52.8	0.319861	3	1.73049	4.9	4.6	7.099	398.5	3.1	0.315724
22	7.264	419	55.6	0.479638	4	3.19343	9.1	8.5	7.264	418.9	3.7	0.480718
23	7.371	433	57.4	0.181806	4	1.09614	3.1	2.9	7.371	433.3	3.5	0.175702
24	7.467	447	59.3	0.414404	4	2.91114	8.3	7.7	7.467	447.3	3.9	0.416813
25	7.735	486	64.5	0.160886	5	1.2831	3.6	3.4	7.735	485.9	4.6	0.158412
26	7.879	507	67.2	0.202963	5	1.71631	4.9	4.5	7.879	506.7	4.7	0.204535
27	8.629	654	86.7	0.141033	6	1.56643	4.5	4.1	8.629	653.7	6.2	0.142968
28	8.774	674	89.4	0.0564769	11	1.0378	3	2.7	8.774	675.6	10.9	0.0538955
29	9.083	709	94	0.0862416	7	0.990265	2.8	2.6	9.083	708.6	6.6	0.0850598

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.0687215	3	0.377165	3.2	2.3	4.55	18.9	3.1	0.0690516
2	5.2	111	14.8	0.120272	4	0.754662	6.4	4.6	5.2	110.7	3.7	0.114993
3	5.85	203	27	0.178618	3	1.01123	8.5	6.2	5.85	203.1	3.3	0.173221
4	6.55	321	42.7	0.0789732	10	1.34984	11.4	8.3	6.55	320.4	10.3	0.0738838
5	6.85	364	48.4	0.062282	5	0.547909	4.6	3.4	6.85	365.1	5.4	0.0575354
6	7.35	427	56.8	0.151897	5	1.19214	10	7.3	7.35	426.6	4.5	0.148508
7	8.15	547	72.7	0.166509	7	1.93263	16.3	11.8	8.15	547.2	6.8	0.161249
8	8.45	604	80.3	0.147985	10	2.56665	21.6	15.7	8.45	601.7	10.1	0.14332
9	8.65	658	87.5	0.126043	5	1.12809	9.5	6.9	8.65	658.4	5.1	0.124461
10	9.3	733	97.5	0.121624	5	1.02299	8.6	6.3	9.3	732.2	4.8	0.120899

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 122

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.700 5.200 7.200 5.850 10.800 6.550 6.600 6.850 5.400 7.350 9.800 8.150 15.800 8.450 21.700 8.650 9.300 9.300 7.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.646 0.800 4.809 3.700 4.934 3.000 5.111 2.300 5.342 4.400 5.420 0.400 5.534 1.500 5.676 0.900 5.740 1.200 5.947 3.100 6.026 3.200 6.089 1.600 6.214 3.600 6.309 2.500 6.335 1.400 6.403 1.000 6.503 7.000 6.674 6.300 6.771 1.300 6.806 2.100 6.875 1.400 6.989 1.200 7.080 4.700 7.260 8.400 7.377 2.600 7.470 7.400 7.723 3.600 7.870 4.600 8.613 8.300 8.746 3.800 9.067 2.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 31 Return to Lane Navigator
4.550 4.300 5.200 5.900 5.850 9.700 6.550 10.100 6.850 5.100 7.350 7.800 8.150 17.700 8.450 21.600 8.650 9.600 9.300 8.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 1.700 4.646 0.300 4.772 1.500 4.941 5.400 5.200 2.500 5.349 1.700 5.544 1.000 5.671 1.100 5.850 2.700 5.933 1.300 6.017 1.900 6.090 0.400 6.251 2.000 6.340 2.800 6.434 0.600 6.538 7.500 6.745 6.900 6.828 1.200 6.860 5.000 6.915 1.700 7.094 4.400 7.241 8.700 7.350 4.000 7.442 7.200 7.690 2.500 7.888 2.700 8.150 3.600 8.450 4.300 8.607 4.000 8.650 2.700 9.056 4.500 9.300 2.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 32 Return to Lane Navigator
4.666 0.600 4.832 3.700 4.969 2.700 5.135 3.200 5.360 4.400 5.549 2.200 5.679 0.700 5.730 0.900 5.958 2.900 6.047 3.000 6.125 1.200 6.252 4.400 6.341 6.300 6.424 1.200 6.524 6.900 6.726 4.800 6.810 0.600 6.855 3.100 6.918 2.100 7.028 0.900 7.099 4.900 7.264 9.100 7.371 3.100 7.467 8.300 7.735 3.600 7.879 4.900 8.629 4.500 8.774 3.000 9.083 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 29 Return to Lane Navigator
4.550 3.200 5.200 6.400 5.850 8.500 6.550 11.400 6.850 4.600 7.350 10.000 8.150 16.300 8.450 21.600 8.650 9.500 9.300 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Chinook Salmon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Chinook Salmon ---- Go to Display Gel Lane Navigator.