RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Haddock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Melanogrammus aeglefinus
FDA Seafood List Common Name: Haddock
FDA Seafood List Market Name: Haddock
Unique IEF Gel Code: 19930824gel01
Unique Web Code: hdg01Srr
Tissue Aliquot Code: melaaegl 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	31	2.7	0.278006	11	5.11676	14.1	10.7	3.5	32	10.7	0.26946
2	4.55	185	16.4	0.224064	9	3.09101	8.5	6.5	4.55	183.1	8.9	0.196791
3	5.2	261	23.1	0.33924	7	4.16711	11.5	8.7	5.2	259.1	7.2	0.327497
4	5.694	369	32.7	0.060328	5	0.468972	1.3	1	5.694	368.1	4.6	0.057839
5	5.85	403	35.7	0.524856	4	3.49237	9.6	7.3	5.85	403.1	3.8	0.523288
6	6.55	569	50.4	0.343854	5	3.06984	8.5	6.4	6.55	568.6	5.3	0.328357
7	6.85	609	53.9	0.163664	8	1.91181	5.3	4	6.85	609.2	7.5	0.143737
8	7.35	705	62.4	0.304051	6	3.15783	8.7	6.6	7.35	703.8	6	0.29492
9	8.15	869	76.9	0.101052	10	1.72133	4.7	3.6	8.15	868.1	10.1	0.0960746
10	8.45	932	82.5	0.155428	7	1.85125	5.1	3.9	8.45	932.4	6.8	0.153388
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	8.65	975	86.3	0.147068	5	1.386	3.8	2.9	8.65	975.2	5.5	0.143398
12	9.3	1092	96.6	0.494924	8	6.84826	18.9	14.4	9.3	1089.2	8.4	0.45914

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	30	2.6	0.201063	9	3.12341	5.1	4.3	3.5	31.4	9.4	0.187368
2	4.172	126	11.1	0.587814	4	4.34474	7.1	6	4.172	126.4	4	0.610832
3	4.319	147	13	0.404284	4	2.60735	4.3	3.6	4.319	147	3.6	0.40789
4	4.445	165	14.6	0.0203725	4	0.140637	0.2	0.2	4.445	164.8	4	0.020025
5	4.55	180	15.9	0.214985	4	1.487	2.4	2	4.55	180	4.3	0.195822
6	4.819	211	18.6	0.116748	3	0.576937	0.9	0.8	4.819	211.6	2.8	0.115028
7	5.018	234	20.7	0.0780556	6	0.70297	1.2	1	5.018	232.2	6.5	0.0614543
8	5.113	245	21.6	0.0637594	3	0.292214	0.5	0.4	5.113	245.6	3	0.0544673
9	5.2	255	22.5	0.19205	3	1.14468	1.9	1.6	5.2	255	3.3	0.196378
10	5.25	266	23.5	0.394546	5	3.21001	5.3	4.4	5.25	266.5	4.6	0.395342
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.385	296	26.1	0.216639	4	1.45917	2.4	2	5.385	295.9	3.9	0.213408
12	5.493	320	28.2	0.0137639	3	0.0756936	0.1	0.1	5.493	321.1	2.9	0.0149301
13	5.539	330	29.1	0.0427229	2	0.169218	0.3	0.2	5.539	329.9	2.2	0.0430821
14	5.593	342	30.2	0.0217843	3	0.124517	0.2	0.2	5.593	341.7	3.4	0.0206002
15	5.674	360	31.8	0.413424	4	2.70263	4.4	3.7	5.674	360.4	3.7	0.407949
16	5.746	376	33.2	0.446222	4	3.04642	5	4.2	5.746	376.7	4	0.433786
17	5.85	399	35.2	0.329774	6	3.55216	5.8	4.9	5.85	398.8	6	0.333609
18	5.942	421	37.2	0.0266311	4	0.2844	0.5	0.4	5.942	422.4	4.3	0.0369504
19	5.996	434	38.3	0.117765	6	1.18811	2	1.6	5.996	435	6.3	0.106821
20	6.15	471	41.6	0.097608	3	0.451074	0.7	0.6	6.15	471.1	2.6	0.0960616
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.188	480	42.4	0.184597	2	0.798576	1.3	1.1	6.188	479.9	2.4	0.184025
22	6.258	497	43.9	0.193977	5	1.55368	2.6	2.1	6.258	499.5	5.4	0.163607
23	6.308	509	44.9	0.486009	3	2.65332	4.4	3.7	6.308	508.5	3.2	0.472721
24	6.463	546	48.2	0.0986944	14	2.19128	3.6	3	6.463	547.8	13.6	0.0911641
25	6.55	567	50	0.145682	6	1.45887	2.4	2	6.55	565.2	6.3	0.131037
26	6.85	607	53.6	0.128366	7	1.2529	2.1	1.7	6.85	607.2	6.5	0.108565
27	7.35	701	61.9	0.306378	5	2.56969	4.2	3.5	7.35	700.5	4.9	0.29866
28	7.873	807	71.2	0.0487105	6	0.498262	0.8	0.7	7.873	805.8	6.1	0.0458547
29	8.15	863	76.2	0.0727012	10	4.41182	7.3	6.1	8.15	872.7	9.7	0.257824
30	8.45	925	81.6	0.10481	6	1.17059	1.9	1.6	8.45	925.7	6.4	0.103947
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	971	85.7	0.230475	18	6.48603	10.7	8.9	8.65	973.9	17.9	0.203936
32	9.3	1091	96.3	0.387995	8	5.10592	8.4	7	9.3	1088.2	8.2	0.351806

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.129	117	10.3	0.600755	4	4.36283	12.1	10	4.129	117.4	3.9	0.625725
2	4.276	137	12.1	0.396259	4	2.56389	7.1	5.9	4.276	137.6	3.5	0.409537
3	4.473	164	14.5	0.0244583	4	0.144251	0.4	0.3	4.473	165.7	3.6	0.0226887
4	4.608	181	16	0.0261647	7	0.319687	0.9	0.7	4.608	178.2	6.8	0.026378
5	4.761	198	17.5	0.163525	3	0.764797	2.1	1.8	4.761	198.3	2.8	0.155074
6	4.967	221	19.5	0.0855019	6	0.847929	2.4	1.9	4.967	219.3	6.2	0.0774499
7	5.057	231	20.4	0.093121	3	0.44265	1.2	1	5.057	231.2	2.6	0.0957225
8	5.146	241	21.3	0.0737522	2	0.295516	0.8	0.7	5.146	240.9	2.3	0.0739083
9	5.209	249	22	0.514007	3	2.42981	6.8	5.6	5.209	249.5	2.9	0.46757
10	5.287	266	23.5	0.0598955	9	1.6171	4.5	3.7	5.287	256.6	9.4	0.0972546
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.347	279	24.6	0.283228	3	1.55288	4.3	3.6	5.347	279.4	3.1	0.282468
12	5.466	305	26.9	0.0217393	2	0.0944062	0.3	0.2	5.466	305.3	2.5	0.0213196
13	5.508	314	27.7	0.0634894	2	0.227347	0.6	0.5	5.508	314	2.3	0.0555346
14	5.549	323	28.5	0.0399375	7	0.532152	1.5	1.2	5.549	323.4	7.3	0.0409555
15	5.641	343	30.3	0.510983	3	2.57559	7.2	5.9	5.641	343	2.9	0.503587
16	5.714	359	31.7	0.509518	4	3.13079	8.7	7.2	5.714	359.1	3.6	0.494108
17	5.82	382	33.7	0.420526	4	2.50799	7	5.8	5.82	382.6	3.6	0.39617
18	5.915	404	35.7	0.0280886	5	0.256411	0.7	0.6	5.915	402.1	5	0.0290468
19	5.961	415	36.7	0.117287	6	1.10975	3.1	2.5	5.961	415.6	5.7	0.110649
20	6.111	451	39.8	0.0829036	3	0.479527	1.3	1.1	6.111	450.7	3.2	0.0835196
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.145	459	40.5	0.144163	4	1.08389	3	2.5	6.145	459.3	4.1	0.147691
22	6.232	480	42.4	0.0827561	3	0.935735	2.6	2.1	6.232	482.9	3.5	0.152521
23	6.278	491	43.4	0.421403	6	4.33235	12	9.9	6.278	490	6	0.409312
24	6.433	528	46.6	0.1114	9	1.63581	4.5	3.8	6.433	527.5	9	0.102267
25	6.79	586	51.8	0.0155837	18	0.514722	1.4	1.2	6.79	593	18	0.0161337
26	6.942	611	54	0.0245853	6	0.173608	0.5	0.4	6.942	612.2	6.5	0.0151228
27	7.815	785	69.3	0.0432651	8	0.593712	1.6	1.4	7.815	787.1	8.2	0.0410231
28	8.613	958	84.6	0.0339251	7	0.469251	1.3	1.1	8.613	959	7.5	0.0354924

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	31	2.7	0.211559	7	2.61333	9.7	7	3.5	32	7	0.209572
2	4.55	168	14.9	0.31748	5	2.28931	8.5	6.1	4.55	167.7	4.6	0.283451
3	5.2	238	21.1	0.4036	5	3.22535	11.9	8.6	5.2	238.4	4.6	0.399542
4	5.85	377	33.4	0.544934	3	3.17967	11.8	8.5	5.85	376.8	3.2	0.557453
5	6.55	544	48.1	0.378792	4	2.87696	10.7	7.7	6.55	543.5	4.4	0.372618
6	6.85	579	51.2	0.144641	4	1.02522	3.8	2.7	6.85	579.6	4.3	0.133973
7	7.35	675	59.7	0.27368	6	2.73707	10.1	7.3	7.35	674.2	5.8	0.267265
8	8.15	845	74.8	0.107299	10	1.77695	6.6	4.8	8.15	846.2	9.8	0.10214
9	8.45	916	81.1	0.133952	6	1.35607	5	3.6	8.45	916	5.8	0.132547
10	8.65	961	85	0.133018	5	1.25237	4.6	3.4	8.65	960.5	5.4	0.129883
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1090	96.5	0.380001	8	4.67847	17.3	12.5	9.3	1087.4	7.6	0.34722

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.3	0.206288	9	3.21341	5.9	4.9	3.5	38.4	9.4	0.192812
2	4.162	124	11	0.521363	4	4.05894	7.5	6.2	4.162	124.5	4.3	0.533401
3	4.314	144	12.7	0.310688	4	2.29077	4.2	3.5	4.314	144.5	4.1	0.315127
4	4.451	162	14.3	0.034381	5	0.283412	0.5	0.4	4.451	162.2	5	0.0322658
5	4.55	175	15.5	0.146466	5	1.32127	2.4	2	4.55	175	5.3	0.140346
6	4.692	191	16.9	0.0179075	7	0.519868	1	0.8	4.692	184.3	7.5	0.0393217
7	4.835	207	18.3	0.0760325	4	0.548075	1	0.8	4.835	206.3	3.9	0.0787952
8	5.004	226	20	0.0781509	7	1.00596	1.9	1.5	5.004	225.9	7.3	0.0780244
9	5.129	240	21.2	0.0864975	5	0.727585	1.3	1.1	5.129	239.1	5.1	0.08109
10	5.2	248	21.9	0.183286	5	1.44733	2.7	2.2	5.2	248.1	4.6	0.178519
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.251	259	22.9	0.326361	5	2.5505	4.7	3.9	5.251	258.6	4.5	0.316658
12	5.329	276	24.4	0.0205936	9	0.55428	1	0.8	5.329	267.8	8.6	0.0361879
13	5.394	290	25.7	0.122498	5	1.05533	1.9	1.6	5.394	290	4.8	0.124212
14	5.532	320	28.3	0.0150984	4	0.0625809	0.1	0.1	5.532	322.6	3.5	0.0100188
15	5.55	324	28.7	0.0260118	8	0.335433	0.6	0.5	5.55	328.3	8.3	0.022794
16	5.61	337	29.8	0.0168113	5	0.0880743	0.2	0.1	5.61	335.4	4.7	0.0106561
17	5.67	350	31	0.301174	3	1.01863	1.9	1.6	5.67	349.6	3	0.193491
18	5.716	360	31.9	0.380274	8	5.39953	10	8.2	5.716	359.1	8.4	0.361436
19	5.85	389	34.4	0.302295	5	2.6888	5	4.1	5.85	389.3	5.1	0.299588
20	5.921	405	35.8	0.00332911	1	0.00105652	0	0	5.921	404	0.5	0.00101894
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.961	414	36.6	0.0167785	4	0.107463	0.2	0.2	5.961	415.1	4.1	0.0149598
22	6.149	456	40.4	0.0395402	4	0.312382	0.6	0.5	6.149	456.9	4.2	0.042278
23	6.207	469	41.5	0.0778071	5	0.678948	1.3	1	6.207	469.6	5.1	0.0743869
24	6.282	486	43	0.064533	3	0.309421	0.6	0.5	6.282	483.5	2.6	0.0659419
25	6.305	491	43.5	0.362562	4	2.49498	4.6	3.8	6.305	491.7	3.8	0.36666
26	6.465	527	46.6	0.0395087	11	0.92055	1.7	1.4	6.465	531.5	11.2	0.0461949
27	6.55	546	48.3	0.160627	6	1.60039	3	2.4	6.55	545.3	6.1	0.146995
28	6.85	588	52	0.109717	6	1.0402	1.9	1.6	6.85	586.8	6.3	0.0931009
29	6.987	613	54.2	0.015423	18	0.671363	1.2	1	6.987	599.5	18	0.0210435
30	7.213	654	57.9	0.0168084	13	0.692524	1.3	1.1	7.213	667.9	13.4	0.0291196
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.35	679	60.1	0.28277	5	2.25844	4.2	3.4	7.35	679	4.8	0.265943
32	7.89	789	69.8	0.0432103	6	0.467843	0.9	0.7	7.89	787.5	6.5	0.0408929
33	8.15	842	74.5	0.0689575	8	0.931159	1.7	1.4	8.15	841.6	8	0.0657459
34	8.45	908	80.4	0.097366	6	1.09788	2	1.7	8.45	909.2	6.4	0.0963312
35	8.65	958	84.8	0.209762	18	6.19469	11.4	9.5	8.65	961.6	18	0.194169
36	9.3	1087	96.2	0.409403	8	5.27743	9.7	8.1	9.3	1084.4	7.8	0.379586

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.558	48	4.2	0.00950801	2	0.0268453	0.1	0.1	3.558	48.7	1.6	0.00929596
2	4.168	127	11.2	0.639978	4	4.81499	14.3	12	4.168	127.3	4.1	0.666643
3	4.322	147	13	0.460561	4	2.95634	8.8	7.4	4.322	147	3.6	0.468262
4	4.438	162	14.3	0.0136955	3	0.0576938	0.2	0.1	4.438	161.9	2.6	0.0125857
5	4.538	175	15.5	0.0341815	3	0.162135	0.5	0.4	4.538	174.9	3.1	0.0290896
6	4.677	191	16.9	0.0218645	9	0.373721	1.1	0.9	4.677	186.7	9	0.0234638
7	4.835	209	18.5	0.157295	3	0.763043	2.3	1.9	4.835	208.8	2.7	0.156911
8	5.02	230	20.4	0.104107	5	0.927383	2.8	2.3	5.02	228.2	5.4	0.0976666
9	5.117	241	21.3	0.135192	2	0.530279	1.6	1.3	5.117	240.6	2.3	0.130741
10	5.207	252	22.3	0.105402	3	0.65616	1.9	1.6	5.207	251.6	3.4	0.108576
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.247	261	23.1	0.559636	3	2.4123	7.2	6	5.247	260.8	2.5	0.537626
12	5.287	270	23.9	0.0654356	6	0.972397	2.9	2.4	5.287	266.6	6.4	0.0850985
13	5.323	278	24.6	0.0518156	3	0.299156	0.9	0.7	5.323	277.1	3.4	0.0499641
14	5.394	294	26	0.21629	5	1.92429	5.7	4.8	5.394	294.5	4.8	0.22537
15	5.546	328	29	0.0431628	6	0.505397	1.5	1.3	5.546	328.7	6.4	0.0445937
16	5.595	339	30	0.0553444	3	0.300384	0.9	0.7	5.595	339	3.5	0.0487631
17	5.662	354	31.3	0.326401	3	1.42316	4.2	3.5	5.662	354.1	2.6	0.311483
18	5.712	365	32.3	0.375423	6	4.3189	12.8	10.8	5.712	364.7	6.3	0.384944
19	5.77	378	33.5	0.0782222	3	0.386513	1.1	1	5.77	377.9	3	0.0723974
20	5.837	393	34.8	0.317635	3	1.69365	5	4.2	5.837	392.9	3	0.316256
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.912	410	36.3	0.0285248	3	0.149318	0.4	0.4	5.912	409.3	2.9	0.0290604
22	5.956	420	37.2	0.0685895	3	0.329381	1	0.8	5.956	420.3	2.8	0.0674436
23	6.145	463	41	0.0794699	3	0.419551	1.2	1	6.145	463.1	3	0.0779455
24	6.198	475	42	0.15203	3	0.928853	2.8	2.3	6.198	475.5	3.4	0.153991
25	6.277	493	43.6	0.121032	4	0.722183	2.1	1.8	6.277	492.6	3.6	0.113692
26	6.303	499	44.2	0.628692	2	2.80881	8.3	7	6.303	498.9	2.4	0.648685
27	6.392	519	45.9	0.0141625	5	0.109801	0.3	0.3	6.392	518.5	4.7	0.0130674
28	6.466	536	47.4	0.0334532	8	0.422632	1.3	1.1	6.466	536	7.6	0.031397
29	6.82	591	52.3	0.0167854	11	0.274283	0.8	0.7	6.82	587.9	11	0.0141137
30	6.958	615	54.4	0.0226335	7	0.253385	0.8	0.6	6.958	615.8	6.6	0.0215345
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.847	790	69.9	0.0714622	5	0.643991	1.9	1.6	7.847	789.3	5.4	0.0668086
32	8.577	946	83.7	0.0608492	11	1.14446	3.4	2.9	8.577	946.8	10.8	0.0598252

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	44	3.9	0.295535	10	5.1013	14.4	10.7	3.5	46.3	10.5	0.275118
2	4.55	178	15.8	0.255211	7	2.94472	8.3	6.2	4.55	179.9	6.9	0.23929
3	5.2	253	22.4	0.321945	7	3.87595	10.9	8.2	5.2	253.6	7	0.312119
4	5.85	403	35.7	0.446865	5	3.67658	10.3	7.7	5.85	402.8	4.6	0.4533
5	6.55	564	49.9	0.397994	5	3.02459	8.5	6.4	6.55	564	4.5	0.376425
6	6.85	602	53.3	0.241551	6	2.23274	6.3	4.7	6.85	602.7	5.7	0.220914
7	7.35	696	61.6	0.301419	6	2.96465	8.3	6.2	7.35	695.2	6	0.278198
8	8.15	863	76.4	0.0904277	10	1.55243	4.4	3.3	8.15	862.3	10	0.0876131
9	8.45	925	81.9	0.168303	7	2.05528	5.8	4.3	8.45	925	7.1	0.164008
10	8.65	968	85.7	0.121913	7	1.50364	4.2	3.2	8.65	967.2	7.1	0.119274
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1089	96.4	0.449244	9	6.60263	18.6	13.9	9.3	1085.5	8.9	0.41959

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 6.55 label for gel schematic
pI 5.8 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 162

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 14.100 4.550 8.500 5.200 11.500 5.694 1.300 5.850 9.600 6.550 8.500 6.850 5.300 7.350 8.700 8.150 4.700 8.450 5.100 8.650 3.800 9.300 18.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 3 PeakCount: 12 Return to Lane Navigator
3.500 5.100 4.172 7.100 4.319 4.300 4.445 0.200 4.550 2.400 4.819 0.900 5.018 1.200 5.113 0.500 5.200 1.900 5.250 5.300 5.385 2.400 5.493 0.100 5.539 0.300 5.593 0.200 5.674 4.400 5.746 5.000 5.850 5.800 5.942 0.500 5.996 2.000 6.150 0.700 6.188 1.300 6.258 2.600 6.308 4.400 6.463 3.600 6.550 2.400 6.850 2.100 7.350 4.200 7.873 0.800 8.150 7.300 8.450 1.900 8.650 10.700 9.300 8.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 4 PeakCount: 32 Return to Lane Navigator
4.129 12.100 4.276 7.100 4.473 0.400 4.608 0.900 4.761 2.100 4.967 2.400 5.057 1.200 5.146 0.800 5.209 6.800 5.287 4.500 5.347 4.300 5.466 0.300 5.508 0.600 5.549 1.500 5.641 7.200 5.714 8.700 5.820 7.000 5.915 0.700 5.961 3.100 6.111 1.300 6.145 3.000 6.232 2.600 6.278 12.000 6.433 4.500 6.790 1.400 6.942 0.500 7.815 1.600 8.613 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 5 PeakCount: 28 Return to Lane Navigator
3.500 9.700 4.550 8.500 5.200 11.900 5.850 11.800 6.550 10.700 6.850 3.800 7.350 10.100 8.150 6.600 8.450 5.000 8.650 4.600 9.300 17.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator
3.500 5.900 4.162 7.500 4.314 4.200 4.451 0.500 4.550 2.400 4.692 1.000 4.835 1.000 5.004 1.900 5.129 1.300 5.200 2.700 5.251 4.700 5.329 1.000 5.394 1.900 5.532 0.100 5.550 0.600 5.610 0.200 5.670 1.900 5.716 10.000 5.850 5.000 5.921 0 5.961 0.200 6.149 0.600 6.207 1.300 6.282 0.600 6.305 4.600 6.465 1.700 6.550 3.000 6.850 1.900 6.987 1.200 7.213 1.300 7.350 4.200 7.890 0.900 8.150 1.700 8.450 2.000 8.650 11.400 9.300 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 7 PeakCount: 36 Return to Lane Navigator
3.558 0.100 4.168 14.300 4.322 8.800 4.438 0.200 4.538 0.500 4.677 1.100 4.835 2.300 5.020 2.800 5.117 1.600 5.207 1.900 5.247 7.200 5.287 2.900 5.323 0.900 5.394 5.700 5.546 1.500 5.595 0.900 5.662 4.200 5.712 12.800 5.770 1.100 5.837 5.000 5.912 0.400 5.956 1.000 6.145 1.200 6.198 2.800 6.277 2.100 6.303 8.300 6.392 0.300 6.466 1.300 6.820 0.800 6.958 0.800 7.847 1.900 8.577 3.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 8 PeakCount: 32 Return to Lane Navigator
3.500 14.400 4.550 8.300 5.200 10.900 5.850 10.300 6.550 8.500 6.850 6.300 7.350 8.300 8.150 4.400 8.450 5.800 8.650 4.200 9.300 18.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Haddock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Haddock ---- Go to Display Gel Lane Navigator.