Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Gray Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus griseus
FDA Seafood List Common Name: Gray Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940830gel02
Unique Web Code: gsh01Srr
Tissue Aliquot Code: lutjgris H94-002 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5512414.50.072526760.7242439.27.34.55124.26.10.0672422
25.221925.60.081329170.97967712.59.95.2219.46.80.0818195
35.8534940.70.12046251.1096514.111.35.85348.75.20.120585
46.5546454.10.11810571.3458517.113.66.55464.36.90.11081
57.3556165.50.039178580.4868546.24.97.35560.27.50.036567
68.1568880.30.058297190.87102211.18.88.15687.48.90.0554577
78.4575387.90.074696370.860974118.78.45751.56.60.0730616
88.6579993.20.051113450.4301765.54.48.65798.54.90.0495507
99.384198.10.1095161.0541413.410.79.3840.95.80.102698
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.91283.30.35825395.6458116.412.93.9130.59.40.339997
24.3769611.20.037353970.399731.20.94.37695.76.50.0345438
34.63613415.60.52156965.3007715.412.14.636133.95.70.525894
45.3123827.70.022762730.1189890.30.35.31238.42.90.022946
55.40125629.80.41009554.1166811.99.45.401254.85.50.422751
65.53928332.90.037824770.4032791.20.95.539281.86.60.0342582
75.60529634.50.031304780.4644561.31.15.605296.58.10.0322401
85.69231336.40.03056630.1573720.50.45.692312.83.20.0280713
95.91435541.30.44681142.993038.76.85.914355.63.80.448557
10637043.10.31323142.197656.4563704.10.30459
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.05838044.20.2217241.488614.33.46.058380.13.90.215441
126.13339345.80.030377820.126020.40.36.133393.52.30.0310954
136.24941348.10.044818860.4536881.316.249411.15.70.0451122
146.3342749.70.15161740.9838962.92.26.33426.43.70.149481
156.40544051.20.2991541.892615.54.36.405440.33.50.30117
166.492455530.34093331.885065.54.36.492455.23.10.342153
176.544464540.084025130.4324851.316.5444642.90.0844575
186.67448055.90.070929530.3784731.10.96.6744803.10.0693622
196.74848956.90.13040940.8729742.526.748488.43.60.136328
206.79749557.60.27729831.249583.62.96.797495.32.50.280178
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.87250458.70.020513920.06137020.20.16.872504.11.80.0194637
227.111533620.18814931.010152.92.37.111533.230.191834
237.36356465.70.091959111.7127553.97.363563.710.80.089083
247.64260870.80.019693350.1729560.50.47.642607.85.30.0183723
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5511713.60.084008260.826749.97.74.55117.660.0776253
25.221224.70.13303440.95266811.48.95.2211.74.20.126662
35.8533739.20.14960741.169341410.95.85336.74.40.14883
46.55464540.12786961.248721511.66.554645.70.123389
57.3556165.30.048299170.5125846.24.87.355616.80.0424503
68.1568679.90.0574301100.97187511.79.18.15687.6100.054853
78.4575287.50.08500371.058912.79.98.45751.57.20.0826374
88.6579792.80.062672250.5187646.24.88.657974.80.0609664
99.383997.70.10441361.0736612.9109.3837.86.30.0967054
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.821283.30.25297693.8164112.110.43.82129.990.239188
24.2719110.60.018800390.2564010.80.74.27191.990.0160334
34.5513015.20.43463764.4626514.212.24.55130.45.70.440018
45.222125.80.026870250.2532710.80.75.2221.25.30.0267298
55.28523627.60.008774810005.28523010
65.36525029.20.33423663.46743119.45.365250.85.50.355147
75.50727532.10.0560151111.000913.22.75.507274.710.90.0518474
85.598291340.037530890.5027231.61.45.598291.290.0316267
95.67230435.50.026782450.2054220.70.65.672304.44.90.0234972
105.94235241.10.29926152.68328.57.35.942350.95.30.285023
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.03736943.10.21031351.86285.95.16.037368.25.20.202922
126.09738044.40.15936841.152973.73.16.097380.14.10.157584
136.18139546.10.020158220.09487180.30.36.181394.32.50.0216627
146.27541248.10.019366750.172540.50.56.275412.34.90.019716
156.35842749.90.10348140.6978922.21.96.358427.23.80.104937
166.4344051.40.23256141.478884.746.43440.13.50.236669
176.51345553.20.27690331.692815.44.66.513454.53.50.275793
186.56946454.20.072734740.5212891.71.46.569464.14.20.070252
196.69479560.0510805142.252427.26.16.69490.214.10.090432
206.763488570.12515340.5390751.71.56.763491.94.40.0690545
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.82749657.90.23851220.5694421.81.66.827496.72.20.149186
226.9505590.062680450.2744330.90.76.9504.45.30.0290138
237.13553462.40.12621230.7609072.42.17.135533.53.40.125789
247.377565660.1095881.515324.84.17.377563.98.40.102157
257.63560670.80.010980950.09124580.30.27.635604.85.20.0099368
268.1568880.40.015271110.2985820.90.88.15690.910.90.0153933
278.4575688.30.027661160.2729990.90.78.457555.80.026739
288.6579993.30.032958380.4192621.31.18.65798.27.90.030093
299.3839980.01938740.1353170.40.49.3838.93.80.0200124
PeakCounter: 29
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.8692630.353214105.7124316.2133.86928.19.70.331546
24.3289210.80.03407860.35501210.84.328926.20.0321962
34.60613215.40.50270654.8036513.710.94.606131.95.30.509716
45.33524228.30.030194430.149420.40.35.335241.52.80.0299601
55.41225629.90.46337253.9029511.18.95.412255.84.70.471232
65.53327832.50.05320850.4534031.315.5332785.20.0487264
75.61529334.30.060776890.9333472.72.15.615292.18.70.0606156
85.68730635.80.045254230.2554850.70.65.687306.23.40.0425976
95.9335040.90.50997533.021758.66.95.933503.40.499462
106.01936642.80.3505742.255776.45.16.019366.33.70.340853
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.08637844.20.24697841.643454.73.76.086377.93.90.236803
126.16439245.80.031174420.1297260.40.36.1643922.40.031012
136.2741148.10.039910160.3972791.10.96.27410.95.80.0389384
146.35342649.80.15961941.007022.92.36.353425.73.60.155717
156.42643951.30.32406131.962385.64.56.426439.13.40.326105
166.504453530.37307931.986395.64.56.504452.930.372073
176.56346354.20.062929330.473891.31.16.563461.23.20.0825426
186.67447755.80.074883630.415121.20.96.674476.93.30.0717257
196.753487570.11386640.9004862.626.753485.43.50.143912
206.79349257.50.33133821.36163.93.16.793492.12.30.334568
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.85650058.50.03625120.1076210.30.26.856499.91.60.0388464
227.1153262.20.16625530.9922.82.37.11532.23.30.167653
237.33256065.50.0894728121.843975.24.27.332560.511.90.0871239
247.65461171.50.015167440.1081510.30.27.654610.94.10.0148445
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5511913.90.081944960.8113559.974.55118.95.90.0770484
25.221525.10.093316771.1302613.89.75.2215.27.20.0883426
35.8533739.40.13844551.1902214.510.25.85336.350.135573
46.55462540.095959981.235411510.66.55460.37.90.0879823
57.3556566.10.040816660.4413535.43.87.35565.16.30.0396468
68.1569481.20.046071100.8122669.978.15692.49.90.0460779
78.4575588.30.085805171.0574612.99.18.45755.470.084792
88.6580193.70.062563850.5392486.64.68.65800.25.10.0601331
99.3838980.097470861.0001812.28.69.3837.26.40.0885232
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 104
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 29 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln