RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19950831gel10
Unique Web Code: esh02Srr
Tissue Aliquot Code: pleuvetu 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	66	8	0.0265517	5	0.236097	9.8	6.3	4.55	66.5	5.2	0.025547
2	5.2	158	19.2	0.0208864	6	0.21341	8.9	5.7	5.2	158.1	5.9	0.0202857
3	5.85	257	31.2	0.0257594	6	0.24199	10.1	6.5	5.85	256.6	5.6	0.0244439
4	6.55	364	44.1	0.0207737	11	0.344986	14.4	9.3	6.55	360.1	10.8	0.0180886
5	7.35	454	55	0.0119766	5	0.110209	4.6	3	7.35	451.7	5.4	0.0115562
6	8.15	568	68.8	0.0386944	7	0.456253	19	12.3	8.15	567	7.1	0.0361119
7	8.45	641	77.7	0.0373078	7	0.464941	19.3	12.5	8.45	641.1	7.4	0.0355635
8	8.65	698	84.6	0.0344584	6	0.335074	13.9	9	8.65	698.7	5.7	0.0329212

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.37	41	5	0.0351059	7	0.422243	4.5	3	4.37	38.4	6.8	0.0347795
2	4.467	55	6.7	0.224205	7	2.75155	29.3	19.9	4.467	57.1	7.3	0.211386
3	4.999	132	16.1	0.00619243	7	0.0641085	0.7	0.5	4.999	130.1	6.8	0.00528963
4	5.089	145	17.7	0.0117814	3	0.0583278	0.6	0.4	5.089	144.4	3	0.0110299
5	5.233	166	20.2	0.0157326	3	0.0887427	0.9	0.6	5.233	165.9	3.2	0.0157567
6	5.423	195	23.8	0.219532	4	1.53256	16.3	11.1	5.423	194.8	4	0.218831
7	5.495	206	25.1	0.0895511	5	0.728687	7.8	5.3	5.495	205.8	4.8	0.0849667
8	5.581	219	26.7	0.0170921	3	0.0843255	0.9	0.6	5.581	220	3	0.0158979
9	5.666	232	28.3	0.260041	3	1.50507	16	10.9	5.666	232.1	3.3	0.257148
10	5.752	245	29.9	0.02477	2	0.10217	1.1	0.7	5.752	245.1	2.4	0.0235596
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.83	257	31.3	0.0224301	6	0.187581	2	1.4	5.83	255	5.6	0.0189469
12	5.889	266	32.4	0.089546	2	0.393561	4.2	2.8	5.889	266.6	2.4	0.0908563
13	6.273	325	39.6	0.0999561	9	1.46958	15.6	10.6	6.273	325.5	9.1	0.090775
14	6.384	342	41.7	0.0226976	1	0.00630406	0.1	0	6.384	342.4	0.5	0.00590845

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	70	8.5	0.0440821	6	0.461319	12.6	8.7	4.55	70.6	6.4	0.040726
2	5.2	166	20.2	0.0395253	6	0.39529	10.8	7.4	5.2	166.3	6.2	0.0360615
3	5.85	265	32.3	0.031403	4	0.236581	6.5	4.4	5.85	264.6	4.3	0.0311207
4	6.55	373	45.5	0.0433995	8	0.57816	15.8	10.8	6.55	372.3	8.3	0.0393574
5	7.35	456	55.6	0.0239403	6	0.256576	7	4.8	7.35	454.1	6.3	0.0228791
6	8.15	566	69	0.0569038	8	0.722889	19.8	13.6	8.15	565.1	7.7	0.0531403
7	8.45	635	77.4	0.0435255	9	0.690312	18.9	12.9	8.45	635.5	9.5	0.0411676
8	8.65	680	82.9	0.0209781	9	0.310354	8.5	5.8	8.65	681	8.6	0.0204538

PeakCounter: 8

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.351	39	4.8	0.016652	6	0.15902	2.5	2	4.351	37.5	5.5	0.0162303
2	4.552	67	8.2	0.121014	6	1.1869	18.5	14.6	4.552	68.4	6	0.111299
3	5.2	157	19.1	0.0162454	4	0.125054	1.9	1.5	5.2	156.8	4.4	0.016022
4	5.262	167	20.4	0.00846498	3	0.0358108	0.6	0.4	5.262	167.6	2.6	0.00790533
5	5.438	195	23.8	0.104154	4	0.643365	10	7.9	5.438	195.4	3.6	0.101476
6	5.512	207	25.2	0.040184	5	0.321879	5	4	5.512	206.7	4.8	0.037467
7	5.594	220	26.8	0.00538361	2	0.0150416	0.2	0.2	5.594	221	1.9	0.0045676
8	5.688	235	28.7	0.156408	4	0.987541	15.4	12.2	5.688	234.7	3.6	0.155449
9	5.762	247	30.1	0.0459211	6	0.450175	7	5.6	5.762	246.3	6.1	0.0417279
10	5.85	261	31.8	0.0231605	3	0.128328	2	1.6	5.85	260.7	3.2	0.0225999
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.916	271	33	0.0574038	3	0.269394	4.2	3.3	5.916	271	2.6	0.058254
12	6.319	332	40.5	0.0727373	9	1.07081	16.7	13.2	6.319	332.7	8.9	0.0676501
13	6.425	348	42.4	0.0361739	4	0.22089	3.4	2.7	6.425	348.2	4.1	0.0303502
14	6.55	367	44.8	0.00424682	2	0.0169433	0.3	0.2	6.55	367.5	2.2	0.00438827
15	7.35	444	54.1	0.0102208	11	0.157874	2.5	1.9	7.35	445.2	10.6	0.00841188
16	8.15	565	68.9	0.0149586	12	0.354866	5.5	4.4	8.15	572	12.1	0.0165423
17	8.45	633	77.2	0.0134283	7	0.144116	2.2	1.8	8.45	633.4	7.3	0.0111867
18	8.65	687	83.8	0.0126868	7	0.128586	2	1.6	8.65	687.2	6.6	0.0109463

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.381	40	4.9	0.0068597	4	0.0528992	0.5	0.4	4.381	39.7	4.2	0.0071331
2	4.468	52	6.3	0.018121	4	0.122869	1.3	0.9	4.468	52	3.6	0.0190948
3	4.584	68	8.3	0.19835	7	2.35534	24.2	17.4	4.584	70.1	7.1	0.188052
4	5.171	149	18.2	0.0112782	4	0.072865	0.7	0.5	5.171	148.6	4.2	0.00974836
5	5.306	170	20.7	0.0126903	3	0.0727231	0.7	0.5	5.306	170	3.4	0.0122272
6	5.375	181	22.1	0.00480592	3	0.0286278	0.3	0.2	5.375	181.9	3.4	0.00473225
7	5.525	205	25	0.161943	5	1.38169	14.2	10.2	5.525	205.7	4.7	0.165814
8	5.6	217	26.5	0.0754476	5	0.594426	6.1	4.4	5.6	216.6	4.6	0.0729341
9	5.669	228	27.8	0.0181989	3	0.100587	1	0.7	5.669	228.2	3.1	0.0184102
10	5.744	240	29.3	0.275888	3	1.43666	14.7	10.6	5.744	239.6	2.9	0.276761
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.8	249	30.4	0.0662295	6	0.738459	7.6	5.4	5.8	250.1	6.5	0.0642294
12	5.916	267	32.6	0.0763085	2	0.336121	3.4	2.5	5.916	266.7	2.4	0.0777852
13	6.304	326	39.8	0.121742	12	2.37651	24.4	17.5	6.304	326	12.3	0.109409
14	6.409	342	41.7	0.038809	2	0.0826107	0.8	0.6	6.409	342.8	2.2	0.0210576

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	60	7.3	0.0322278	5	0.301567	12.1	7.7	4.55	60.4	5.5	0.0310249
2	5.2	149	18.1	0.0280692	5	0.241515	9.7	6.1	5.2	148.6	5.2	0.0259724
3	5.85	253	30.8	0.0256331	6	0.241818	9.7	6.1	5.85	252	5.9	0.0232772
4	6.55	360	43.8	0.0224757	8	0.310067	12.5	7.9	6.55	357.9	8.4	0.0208244
5	7.35	444	54.1	0.0131908	6	0.127745	5.1	3.2	7.35	443.4	6.1	0.0117632
6	8.15	566	68.9	0.0413689	7	0.47094	18.9	12	8.15	565.4	6.8	0.039239
7	8.45	637	77.6	0.0355824	8	0.475823	19.1	12.1	8.45	637.9	7.8	0.0342174
8	8.65	693	84.4	0.0360026	5	0.31896	12.8	8.1	8.65	693.6	5.3	0.0338155

PeakCounter: 8

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 70

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 9.800 5.200 8.900 5.850 10.100 6.550 14.400 7.350 4.600 8.150 19.000 8.450 19.300 8.650 13.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 8 Return to Lane Navigator
4.370 4.500 4.467 29.300 4.999 0.700 5.089 0.600 5.233 0.900 5.423 16.300 5.495 7.800 5.581 0.900 5.666 16.000 5.752 1.100 5.830 2.000 5.889 4.200 6.273 15.600 6.384 0.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 12.600 5.200 10.800 5.850 6.500 6.550 15.800 7.350 7.000 8.150 19.800 8.450 18.900 8.650 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 8 Return to Lane Navigator
4.351 2.500 4.552 18.500 5.200 1.900 5.262 0.600 5.438 10.000 5.512 5.000 5.594 0.200 5.688 15.400 5.762 7.000 5.850 2.000 5.916 4.200 6.319 16.700 6.425 3.400 6.550 0.300 7.350 2.500 8.150 5.500 8.450 2.200 8.650 2.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 18 Return to Lane Navigator
4.381 0.500 4.468 1.300 4.584 24.200 5.171 0.700 5.306 0.700 5.375 0.300 5.525 14.200 5.600 6.100 5.669 1.000 5.744 14.700 5.800 7.600 5.916 3.400 6.304 24.400 6.409 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 14 Return to Lane Navigator
4.550 12.100 5.200 9.700 5.850 9.700 6.550 12.500 7.350 5.100 8.150 18.900 8.450 19.100 8.650 12.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 8 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: English Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.