RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Dover Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Microstomus pacificus
FDA Seafood List Common Name: Dover Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19980828gel01
Unique Web Code: dvg02Srr
Tissue Aliquot Code: micrpaci I94-002 #05p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	81	3.6	0.436058	14	10.4244	7.3	5.7	3.5	81.8	13.6	0.431053
2	4.55	393	17.4	0.468267	8	6.04508	4.2	3.3	4.55	392.4	7.6	0.447031
3	5.2	565	25	1.68113	6	17.1169	12	9.4	5.2	564.4	6.2	1.55818
4	5.85	880	38.9	2.3866	6	26.8102	18.8	14.7	5.85	882.3	5.7	2.64568
5	6.55	1222	54	2.16041	4	15.9984	11.2	8.8	6.55	1222.7	4.1	2.18334
6	6.85	1313	58.1	0.80248	7	9.1318	6.4	5	6.85	1312.5	7.3	0.701012
7	7.35	1499	66.3	2.23621	7	27.2435	19.1	14.9	7.35	1500.8	6.8	2.2591
8	8.15	1815	80.3	0.154209	16	4.39402	3.1	2.4	8.15	1812.3	16.3	0.152033
9	8.45	1928	85.3	0.353747	10	6.30301	4.4	3.5	8.45	1927.8	10.2	0.348614
10	8.65	2000	88.5	0.221342	11	4.19341	2.9	2.3	8.65	1999.2	10.9	0.216931
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2224	98.4	1.43051	6	15.2632	10.7	8.4	9.3	2225.1	6.2	1.39826

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.775	169	7.5	2.3781	12	58.2144	21.8	21	3.775	174.1	12.3	2.68077
2	4.147	279	12.3	0.0520527	21	1.71046	0.6	0.6	4.147	277.5	20.5	0.0470451
3	4.298	324	14.3	0.286347	10	5.36527	2	1.9	4.298	322.7	10.2	0.296103
4	4.597	411	18.1	1.86882	12	37.3391	14	13.5	4.597	410	11.6	1.8133
5	4.858	480	21.2	0.0913969	15	2.43796	0.9	0.9	4.858	476.6	14.8	0.092703
6	4.96	507	22.4	0.0958873	14	2.30942	0.9	0.8	4.96	508	14.4	0.0906565
7	5.138	554	24.4	0.0564463	14	1.40299	0.5	0.5	5.138	549.2	13.6	0.0581686
8	5.322	629	27.7	0.0390367	14	0.966706	0.4	0.3	5.322	628.1	14.2	0.0384776
9	5.51	719	31.7	0	1	0	0	0	5.51	718	1	0
10	5.539	733	32.3	0.509719	4	3.65168	1.4	1.3	5.539	732.4	4.3	0.478478
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.581	753	33.2	2.21281	12	48.5761	18.2	17.5	5.581	756.8	12.2	2.252
12	5.629	776	34.2	2.16133	6	22.4516	8.4	8.1	5.629	777.8	6	2.10844
13	5.698	809	35.7	0.172019	7	2.04771	0.8	0.7	5.698	809.6	6.5	0.177502
14	5.741	830	36.6	0.0469433	27	1.37188	0.5	0.5	5.741	837.7	27	0.0286576
15	5.777	847	37.3	1.84772	6	16.8294	6.3	6.1	5.777	846.8	5.8	1.64274
16	5.866	890	39.2	2.24444	12	47.2197	17.7	17.1	5.866	886.4	12.5	2.13643
17	6.016	965	42.5	0.172981	7	1.93537	0.7	0.7	6.016	963.2	7.1	0.153818
18	6.088	1001	44.1	0.0787886	5	0.627461	0.2	0.2	6.088	1001.5	4.7	0.0747986
19	6.284	1099	48.5	0.0853147	24	3.40177	1.3	1.2	6.284	1099.8	23.7	0.0809959
20	6.366	1140	50.3	0.111808	13	2.35816	0.9	0.9	6.366	1140.1	13	0.102662
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.424	1169	51.5	0.160421	11	2.91622	1.1	1.1	6.424	1168.5	11.2	0.146419
22	6.596	1246	54.9	0.211455	4	1.36741	0.5	0.5	6.596	1246.3	3.7	0.210361
23	7.064	1400	61.7	0.0303466	6	0.324761	0.1	0.1	7.064	1400.7	5.9	0.0312776
24	7.267	1473	64.9	0.038018	23	1.51616	0.6	0.5	7.267	1470.8	22.8	0.0374667
25	7.317	1491	65.7	0.039722	5	0.11448	0	0	7.317	1490.6	4.7	0.0138308

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	76	3.4	0.58943	16	15.0998	10.3	7.9	3.5	78.9	16	0.533518
2	4.55	386	17.1	0.982957	5	7.62503	5.2	4	4.55	385.3	5.4	0.801421
3	5.2	558	24.7	1.41992	7	18.6403	12.7	9.8	5.2	556.4	7.4	1.41572
4	5.85	866	38.4	2.3875	6	25.5207	17.4	13.4	5.85	867.4	5.8	2.49646
5	6.55	1224	54.2	2.06618	5	17.996	12.3	9.5	6.55	1224.9	5	2.04112
6	6.85	1315	58.3	1.16569	5	9.2834	6.3	4.9	6.85	1314.8	5.5	0.956742
7	7.35	1489	66	2.20617	6	23.128	15.8	12.2	7.35	1491.2	5.8	2.25369
8	8.15	1797	79.6	0.135135	14	3.42304	2.3	1.8	8.15	1796.9	14.2	0.135934
9	8.45	1913	84.8	0.322013	10	5.35601	3.7	2.8	8.45	1912.2	9.6	0.315982
10	8.65	1986	88	0.23703	9	3.68335	2.5	1.9	8.65	1985	8.9	0.232622
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2211	98	2.0181	5	16.6952	11.4	8.8	9.3	2210	4.7	2.02412

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	82	3.6	0.284369	23	9.9624	2.6	2.5	3.5	85.7	22.8	0.246012
2	3.801	170	7.5	2.35814	12	58.4808	15.3	14.5	3.801	163.2	12.1	2.72282
3	4.143	270	11.9	0.0814292	10	1.2766	0.3	0.3	4.143	269.5	10	0.0717002
4	4.287	312	13.8	0.50122	6	5.23983	1.4	1.3	4.287	311.2	6.3	0.467623
5	4.437	356	15.7	0.0274938	9	0.433439	0.1	0.1	4.437	354.1	9	0.0270984
6	4.55	389	17.2	2.30043	8	39.3749	10.3	9.7	4.55	393.9	8.4	2.63149
7	4.819	461	20.4	0.176371	9	2.48757	0.6	0.6	4.819	460.6	8.6	0.162992
8	4.95	496	21.9	0.209085	11	4.21367	1.1	1	4.95	495.4	11.5	0.207442
9	5.092	534	23.6	0.147613	10	2.16834	0.6	0.5	5.092	535.4	10.2	0.119486
10	5.2	563	24.9	1.66185	4	10.9543	2.9	2.7	5.2	562.2	3.9	1.60292
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.289	607	26.8	0.0630168	11	1.19827	0.3	0.3	5.289	610.4	10.8	0.0625043
12	5.469	696	30.7	0.0399364	5	0.35357	0.1	0.1	5.469	694.7	5.4	0.0369118
13	5.514	718	31.7	1.05557	3	4.81845	1.3	1.2	5.514	717.6	2.7	0.997105
14	5.54	731	32.3	2.19348	11	46.8031	12.2	11.6	5.54	736.8	10.7	2.46347
15	5.588	755	33.3	2.12485	7	29.9756	7.8	7.4	5.588	759	7.4	2.29021
16	5.663	792	35	0.522811	5	4.4637	1.2	1.1	5.663	792.8	5.1	0.496569
17	5.708	814	36	0.156813	4	1.05892	0.3	0.3	5.708	813.7	3.6	0.167427
18	5.736	828	36.6	2.14272	5	18.1677	4.7	4.5	5.736	828.6	4.8	2.15176
19	5.829	874	38.6	2.22491	14	63.6819	16.6	15.7	5.829	865	14.5	2.4808
20	5.924	920	40.6	0.0283281	7	0.346394	0.1	0.1	5.924	921.1	7.4	0.0264648
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.993	953	42.1	0.176989	5	1.40889	0.4	0.3	5.993	952.3	4.8	0.166588
22	6.07	990	43.7	0.0766804	5	0.688564	0.2	0.2	6.07	990.9	5.4	0.0718839
23	6.283	1092	48.2	0.0558826	16	1.55821	0.4	0.4	6.283	1092.2	16	0.0548353
24	6.394	1145	50.6	0.086571	11	1.66048	0.4	0.4	6.394	1144	10.7	0.0876634
25	6.452	1173	51.8	0.168614	15	3.81893	1	0.9	6.452	1173.7	15.3	0.140942
26	6.55	1220	53.9	0.325072	10	5.34902	1.4	1.3	6.55	1217.1	10.1	0.300255
27	6.681	1262	55.7	0.256958	3	1.52511	0.4	0.4	6.681	1261.5	3.4	0.252786
28	6.85	1316	58.1	0.536049	7	5.97974	1.6	1.5	6.85	1315.2	6.8	0.498345
29	6.904	1335	59	0.0693521	8	1.09931	0.3	0.3	6.904	1332.8	8.4	0.0737708
30	7.127	1414	62.5	0.0338102	8	0.449557	0.1	0.1	7.127	1416.6	8.2	0.0308684
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.26	1461	64.5	0.0516043	24	2.26551	0.6	0.6	7.26	1477.6	24.2	0.0528398
32	7.35	1493	65.9	2.27737	6	28.9785	7.6	7.2	7.35	1489.9	6.5	2.52474
33	8.15	1796	79.3	0.0312606	7	0.380473	0.1	0.1	8.15	1796	7.5	0.0286305
34	8.45	1912	84.5	0.188984	13	4.24997	1.1	1.1	8.45	1912	13.4	0.179301
35	8.65	1986	87.7	0.167452	10	2.79621	0.7	0.7	8.65	1984.5	9.9	0.159987
36	9.217	2185	96.5	0.210207	21	7.33212	1.9	1.8	9.217	2196.8	20.7	0.199561
37	9.3	2214	97.8	1.16195	5	7.79659	2	1.9	9.3	2213.5	4.9	0.906149

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 20
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.767	158	7	2.383	12	58.9968	22.1	21.2	3.767	162.5	12.4	2.69218
2	4.163	277	12.2	0.0615839	10	0.994773	0.4	0.4	4.163	275.2	10.5	0.0535562
3	4.302	319	14.1	0.350121	7	3.91736	1.5	1.4	4.302	318	6.6	0.334661
4	4.577	401	17.7	2.35157	8	35.8782	13.4	12.9	4.577	403.5	7.7	2.61301
5	4.839	473	20.9	0.120413	11	2.20672	0.8	0.8	4.839	473	11.1	0.111962
6	4.955	505	22.3	0.127854	10	2.33922	0.9	0.8	4.955	503.4	10.3	0.128279
7	5.1	545	24.1	0.0780251	8	1.0342	0.4	0.4	5.1	545.8	8.5	0.0688165
8	5.297	621	27.4	0.046816	12	1.00315	0.4	0.4	5.297	625.2	11.7	0.0485314
9	5.534	739	32.6	0.492371	3	2.13824	0.8	0.8	5.534	738.8	2.8	0.429563
10	5.568	756	33.4	2.22067	10	42.5446	15.9	15.3	5.568	758.3	9.8	2.45527
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.622	783	34.6	2.10471	7	24.9493	9.3	9	5.622	782.5	7	2.02333
12	5.686	815	36	0.141726	6	1.61143	0.6	0.6	5.686	815	6.1	0.148517
13	5.728	836	36.9	0.0896191	4	0.58197	0.2	0.2	5.728	836	3.6	0.0923668
14	5.762	853	37.7	1.32149	7	13.2777	5	4.8	5.762	855.7	6.5	1.14613
15	5.845	894	39.5	2.22362	14	58.5627	21.9	21.1	5.845	888.5	13.7	2.41061
16	6.002	970	42.8	0.114597	10	2.03272	0.8	0.7	6.002	969.4	10.4	0.110086
17	6.095	1015	44.8	0.0588929	10	1.05833	0.4	0.4	6.095	1014.6	9.9	0.0605517
18	6.287	1108	48.9	0.122791	22	4.69109	1.8	1.7	6.287	1109.8	22.1	0.119603
19	6.38	1153	50.9	0.122127	15	3.03627	1.1	1.1	6.38	1153.1	14.7	0.11659
20	6.449	1186	52.4	0.183187	15	4.76393	1.8	1.7	6.449	1185.6	15	0.178973
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.651	1267	55.9	0.110112	7	1.4028	0.5	0.5	6.651	1265.8	6.8	0.115625
22	7.103	1423	62.8	0.0263544	9	0.424976	0.2	0.2	7.103	1422.5	9.1	0.0263869

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 21
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 22
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	73	3.2	0.451287	14	10.7482	7	5.6	3.5	76.3	13.9	0.437837
2	4.55	398	17.6	0.638295	7	7.18139	4.7	3.8	4.55	399.8	7	0.575247
3	5.2	582	25.7	1.80963	6	18.2782	11.9	9.6	5.2	582.7	6.3	1.63191
4	5.85	909	40.1	2.38724	6	30.43	19.8	16	5.85	905.4	6.3	2.72358
5	6.55	1250	55.2	2.13787	5	16.8155	10.9	8.8	6.55	1250.8	4.6	2.04793
6	6.85	1345	59.4	0.759214	7	8.42493	5.5	4.4	6.85	1343.8	7.4	0.646174
7	7.35	1534	67.7	2.35113	7	29.0509	18.9	15.2	7.35	1533.8	6.6	2.47264
8	8.15	1829	80.8	0.163374	15	4.15214	2.7	2.2	8.15	1829.7	14.7	0.158941
9	8.45	1940	85.7	0.403245	9	6.24267	4.1	3.3	8.45	1939.6	9	0.391427
10	8.65	2011	88.8	0.242638	9	3.84567	2.5	2	8.65	2010	9.1	0.237966
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2234	98.6	2.23936	5	18.4504	12	9.7	9.3	2233.5	4.7	2.20429

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 117

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 7.300 4.550 4.200 5.200 12.000 5.850 18.800 6.550 11.200 6.850 6.400 7.350 19.100 8.150 3.100 8.450 4.400 8.650 2.900 9.300 10.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 11 Return to Lane Navigator
3.775 21.800 4.147 0.600 4.298 2.000 4.597 14.000 4.858 0.900 4.960 0.900 5.138 0.500 5.322 0.400 5.510 0 5.539 1.400 5.581 18.200 5.629 8.400 5.698 0.800 5.741 0.500 5.777 6.300 5.866 17.700 6.016 0.700 6.088 0.200 6.284 1.300 6.366 0.900 6.424 1.100 6.596 0.500 7.064 0.100 7.267 0.600 7.317 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 18 PeakCount: 25 Return to Lane Navigator
3.500 10.300 4.550 5.200 5.200 12.700 5.850 17.400 6.550 12.300 6.850 6.300 7.350 15.800 8.150 2.300 8.450 3.700 8.650 2.500 9.300 11.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 19 PeakCount: 11 Return to Lane Navigator
3.500 2.600 3.801 15.300 4.143 0.300 4.287 1.400 4.437 0.100 4.550 10.300 4.819 0.600 4.950 1.100 5.092 0.600 5.200 2.900 5.289 0.300 5.469 0.100 5.514 1.300 5.540 12.200 5.588 7.800 5.663 1.200 5.708 0.300 5.736 4.700 5.829 16.600 5.924 0.100 5.993 0.400 6.070 0.200 6.283 0.400 6.394 0.400 6.452 1.000 6.550 1.400 6.681 0.400 6.850 1.600 6.904 0.300 7.127 0.100 7.260 0.600 7.350 7.600 8.150 0.100 8.450 1.100 8.650 0.700 9.217 1.900 9.300 2.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 19 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 20 PeakCount: 37 Return to Lane Navigator
3.767 22.100 4.163 0.400 4.302 1.500 4.577 13.400 4.839 0.800 4.955 0.900 5.100 0.400 5.297 0.400 5.534 0.800 5.568 15.900 5.622 9.300 5.686 0.600 5.728 0.200 5.762 5.000 5.845 21.900 6.002 0.800 6.095 0.400 6.287 1.800 6.380 1.100 6.449 1.800 6.651 0.500 7.103 0.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 20 LANE TYPE: Specimen ACTUAL GEL LANE: 21 PeakCount: 22 Return to Lane Navigator
3.500 7.000 4.550 4.700 5.200 11.900 5.850 19.800 6.550 10.900 6.850 5.500 7.350 18.900 8.150 2.700 8.450 4.100 8.650 2.500 9.300 12.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 21 LANE TYPE: pI Markers ACTUAL GEL LANE: 22 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Dover Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Dover Sole ---- Go to Display Gel Lane Navigator.