Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Spotfin Croaker  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Roncador stearnsi
FDA Seafood List Common Name: Spotfin Croaker
FDA Seafood List Market Name: Croaker
Unique IEF Gel Code: 19971029gel10
Unique Web Code: crh01Srr
Tissue Aliquot Code: roncstea I96-003 #05p Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55374.70.07384450.56159585.94.5537.54.60.0683899
25.2127160.033902780.4023335.74.25.2127.57.50.0301446
35.8524831.20.04250930.2166963.12.35.85247.730.0411785
46.5539249.30.081651970.9595313.610.16.55389.47.30.0738992
56.8542753.70.032685280.42015264.46.85426.98.30.0287006
67.3550062.90.055769250.4166715.94.47.35499.44.60.0512597
78.1562278.20.090010381.2135117.212.88.156228.10.0845393
88.4568986.70.12915381.7381824.718.38.45687.57.90.12397
98.6573692.60.11390640.78842911.28.38.65735.64.10.108619
109.377697.60.041649650.3297154.73.59.3775.24.70.0396679
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.423212.60.3685342.5513611.88.84.42320.53.90.3707
25.29514518.20.099150730.5165672.41.85.295144.630.0980448
35.40716520.70.043293520.1635240.80.65.407164.92.20.0423336
45.47517722.20.42620132.2380910.47.75.475176.630.4222
55.53118723.50.21954162.102659.77.25.531186.65.70.20963
65.59219824.90.066342540.42992621.55.592198.540.0610491
75.823529.50.061038840.3690631.71.35.8234.83.50.0586892
85.93426232.90.049456530.2770261.315.934262.23.20.0487094
95.98127234.20.061227530.350161.61.25.981271.93.40.0575991
106.07429236.70.1267291.75588.166.074293.28.90.111876
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.18631639.70.14414361.425146.64.96.186314.46.50.123744
126.28433742.30.48640932.6049712.196.284337.430.491429
136.33534843.70.096850340.7369643.42.56.335347.34.50.0931902
146.36835544.60.48769732.38464118.26.368355.32.80.489082
156.52238848.70.02133630.1221380.60.46.522387.43.10.0222263
166.59539950.10.014190530.07964620.40.36.5953993.30.0136925
176.71441251.80.021282140.1646930.80.66.7144124.40.0212592
186.84142653.50.043159760.43056821.56.841426.66.10.0398698
196.94744155.40.03598740.2298581.10.86.947440.540.032424
207.06445857.50.346531.571687.35.47.064458.32.60.343218
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.46969387.10.0558362121.095295.13.88.469693.712.20.0505948
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55425.20.068615350.51948285.74.5542.44.90.060118
25.213116.40.044503980.5368718.35.95.2131.17.50.0403788
35.8524230.20.027498940.1989043.12.25.85241.94.20.0269124
46.5539849.80.12101961.2581319.513.96.55397.56.20.114867
56.8542953.60.019290780.2333963.62.66.85429.27.90.0166524
67.3550162.60.039932950.3013264.73.37.35500.54.90.034775
78.1562778.40.065688980.90585614108.15627.18.30.0617858
88.4569086.20.11691371.5181623.516.88.456897.50.11428
98.65736920.099845240.71678811.17.98.65735.94.20.095676
109.377596.90.037056740.265084.12.99.3774.54.30.0349417
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.412273.40.21772841.650559.27.34.412274.30.217264
24.55445.50.023110430.150250.80.74.5544.53.50.0243098
35.212415.50.018036960.194061.10.95.2123.66.30.017333
45.30314317.90.038055950.3086221.71.45.303142.64.60.0381753
55.394160200.029966340.2116481.20.95.394159.840.0296275
65.45417121.40.25047651.680559.47.45.454170.94.70.201443
75.524184230.11757992.1674912.19.65.524179.68.70.139829
85.58419524.40.029850190.35614521.65.584194.98.50.0236335
95.783232290.041526260.4235022.41.95.783231.75.80.0410911
105.89825531.90.035804940.2628291.51.25.898254.54.20.0349009
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.939264330.047589530.2899051.61.35.9392643.50.0468414
125.99827734.60.032499220.03254640.20.15.998276.62.20.0083685
136.03428535.60.05295790.730814.13.26.0342858.90.0465494
146.12530538.10.011669830.04299670.20.26.125306.32.90.0084988
156.17931739.60.070327150.5422632.46.179317.64.70.0651185
166.25633441.80.015529470.1586110.90.76.256331.870.0127695
176.34235344.10.4424132.2599912.6106.342353.12.90.436127
186.39636545.60.057229970.6317073.52.86.396365.67.10.0503459
196.43237346.60.40489121.681249.47.56.432373.52.40.389841
206.53239549.40.035368930.1944991.10.96.532394.63.10.0356713
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.6240650.80.010062630.05035170.30.26.62406.23.30.0086196
226.7141551.90.020155130.1017190.60.56.71415.63.20.0181488
236.8442853.50.040629660.4268862.41.96.84427.56.50.0372424
246.92944155.10.023178840.131610.70.66.929440.73.60.0204792
257.04145857.20.26490431.197046.75.37.0414582.60.26214
267.3550563.10.014677780.1930571.10.97.35504.380.0135644
278.1563379.10.020785670.2364021.318.1563370.0190548
288.45696870.0724658111.19926.75.38.45694.810.80.062468
298.6573992.40.04610750.3817022.11.78.65738.750.0433575
309.3776970.009591640.0593640.30.39.3777.14.10.008081
PeakCounter: 30
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.435293.60.33047142.5047611.67.94.435294.30.33093
25.32215118.80.059788750.4931082.31.65.322150.84.70.0597764
35.4317121.30.021491920.09128160.40.35.43170.32.30.0220774
45.518422.90.32027942.299810.67.25.5183.34.10.318401
55.549193240.18713651.7345785.55.549193.35.40.182516
65.61420525.50.052147350.3990911.81.35.614204.84.50.0500148
75.79823929.80.076276740.4895732.31.55.798239.53.60.0758196
85.90626132.50.043266130.21050810.75.906260.82.80.0430331
95.94326933.50.049625130.2645671.20.85.943269.13.10.0475322
106.00228235.10.01695430.1452640.70.56.002283.83.10.02617
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.04829236.40.060072950.5079612.31.66.048292.24.70.0611701
126.0829937.20.031855330.1394510.60.46.08299.13.10.0255581
136.19832540.50.092181160.8943294.12.86.198324.55.80.0877505
146.253337420.016278440.1068820.50.36.253337.23.80.0158304
156.33535544.20.478432.6993312.58.56.335355.23.20.474838
166.39536845.80.077004750.65608632.16.395367.45.30.0705079
176.42737546.70.4194521.7351685.56.427375.42.30.417305
186.53239849.60.030099830.1537620.70.56.532398.22.80.0304542
196.62240950.90.015228730.0681450.30.26.6224092.60.0147188
206.72641952.20.019947230.1057890.50.36.726419.630.0200178
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.86343353.90.036429240.1736560.80.56.863432.83.60.0268558
226.95344755.70.0469126130.7652663.52.46.953447.412.80.0336244
237.06246457.80.34199131.602157.457.062463.72.70.333689
248.46670187.30.123088183.4038615.710.78.466703.7180.106692
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55425.20.061651760.56174396.14.55435.60.0567141
25.213116.30.030198680.3978936.44.35.2132.580.0281925
35.8525131.20.032800270.2724364.435.85249.66.60.0233316
46.5540350.10.093645281.2914520.7146.55401.78.30.0873719
57.3551163.60.026748240.185573327.35511.14.20.0246964
68.1563378.70.062929490.96268515.410.58.15633.19.20.0593507
78.4569786.70.11784371.4909423.916.28.45696.87.30.115136
88.6574492.50.096669740.69400611.17.58.65743.34.20.0937619
99.378597.60.049215550.37513164.19.3784.74.50.0468872
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 104
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 30 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln