RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Coho Salmon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Oncorhynchus kisutch
FDA Seafood List Common Name: Coho Salmon
FDA Seafood List Market Name: Salmon, Coho (Silver, Medium Red)
Unique IEF Gel Code: 19940906gel01
Unique Web Code: cog01Srr
Tissue Aliquot Code: oncokisu H94-002 #01m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.125312	10	2.17342	11.8	8.9	3.5	35.4	10.4	0.118287
2	4.55	182	16	0.200118	5	1.39832	7.6	5.7	4.55	182.4	4.6	0.171764
3	5.2	262	23.1	0.226643	5	1.76397	9.6	7.2	5.2	261.7	4.7	0.213896
4	5.85	403	35.5	0.400322	3	1.80059	9.8	7.4	5.85	402.7	2.5	0.406239
5	6.55	572	50.4	0.234291	4	1.70512	9.3	7	6.55	571.6	4.3	0.221378
6	6.85	609	53.7	0.0831019	7	0.917638	5	3.8	6.85	609.1	7.1	0.0732958
7	7.35	703	61.9	0.219306	5	1.76391	9.6	7.2	7.35	702.8	4.7	0.212731
8	8.15	864	76.1	0.0733348	11	1.35644	7.4	5.5	8.15	864.1	10.9	0.0703488
9	8.45	933	82.2	0.104367	7	1.37178	7.5	5.6	8.45	933	7.4	0.104722
10	8.65	976	86	0.111147	6	1.06679	5.8	4.4	8.65	976.4	5.6	0.106878
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1108	97.6	0.490193	4	3.03676	16.5	12.4	9.3	1108.2	3.6	0.477388

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.5	0.140835	10	2.38711	5.8	5.1	3.5	41.9	10.3	0.131063
2	4.55	194	17.1	0.153254	4	0.899938	2.2	1.9	4.55	194	3.7	0.137088
3	4.613	202	17.8	0.102091	3	0.546079	1.3	1.2	4.613	201.7	3.1	0.0997058
4	4.954	245	21.6	0.0454891	3	0.218045	0.5	0.5	4.954	245.3	2.9	0.0431284
5	5.049	257	22.6	0.0553488	9	0.755426	1.8	1.6	5.049	259.7	9.4	0.045349
6	5.2	276	24.3	0.157597	5	1.18507	2.9	2.5	5.2	275.8	4.5	0.148161
7	5.276	292	25.7	0.0312483	8	0.318915	0.8	0.7	5.276	296.3	7.8	0.0230416
8	5.376	313	27.6	0.0652255	4	0.462206	1.1	1	5.376	312	4	0.0654104
9	5.47	333	29.3	0.0881198	4	0.400273	1	0.9	5.47	332.2	3.7	0.0609023
10	5.504	340	30	0.153371	6	1.35274	3.3	2.9	5.504	340.1	6	0.126975
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.627	366	32.2	0.0483219	5	0.445853	1.1	1	5.627	366.7	5.4	0.0467629
12	5.689	379	33.4	0.0697678	6	0.60917	1.5	1.3	5.689	379.5	5.6	0.0617565
13	5.85	413	36.4	0.327805	2	1.43976	3.5	3.1	5.85	412.7	2.4	0.333425
14	6.014	450	39.6	0.29167	2	1.25214	3	2.7	6.014	450.6	2.4	0.29767
15	6.107	471	41.5	0.189114	2	0.779561	1.9	1.7	6.107	471	2.5	0.178853
16	6.368	530	46.7	0.39801	3	2.06439	5	4.4	6.368	530	2.9	0.399531
17	6.461	551	48.5	0.253934	2	1.03798	2.5	2.2	6.461	551.1	2.3	0.249456
18	6.51	562	49.5	0.0755432	4	0.453706	1.1	1	6.51	563	3.6	0.0705125
19	6.55	571	50.3	0.209839	3	0.947057	2.3	2	6.55	570.9	2.6	0.203453
20	6.613	579	51	0.222576	2	0.791146	1.9	1.7	6.613	578.9	2	0.218143
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.676	587	51.7	0.260924	3	1.42822	3.5	3	6.676	588.1	3	0.272351
22	6.803	603	53.1	0.0956993	2	0.362829	0.9	0.8	6.803	602.9	2.2	0.093675
23	6.85	609	53.7	0.0347327	2	0.157006	0.4	0.3	6.85	608.6	2.5	0.0358976
24	7.18	667	58.8	0.270251	2	1.00126	2.4	2.1	7.18	667.3	2.1	0.275206
25	7.35	697	61.4	0.181004	5	1.50247	3.7	3.2	7.35	696.3	5	0.168406
26	7.445	715	63	0.255878	2	1.06824	2.6	2.3	7.445	714.8	2.4	0.250924
27	7.587	742	65.4	0.175435	5	1.4017	3.4	3	7.587	741.2	4.6	0.17119
28	7.766	776	68.4	0.0896649	4	0.601264	1.5	1.3	7.766	775.7	3.8	0.089033
29	7.866	795	70	0.195381	5	1.84987	4.5	4	7.866	794.7	5.5	0.19076
30	8.15	849	74.8	0.0653964	10	1.22488	3	2.6	8.15	851	10.3	0.0671548
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.45	922	81.2	0.0988695	6	0.439595	1.1	0.9	8.45	920.6	5.9	0.0418231
32	8.514	938	82.6	0.0879481	18	2.67331	6.5	5.7	8.514	935.4	18	0.0837933
33	8.65	972	85.6	0.253923	12	4.9869	12.1	10.6	8.65	972.2	11.9	0.236147
34	8.789	1000	88.1	0.045466	4	0.22383	0.5	0.5	8.789	1001.1	4.2	0.0300188
35	8.903	1023	90.1	0.0503006	6	0.524909	1.3	1.1	8.903	1023	6.3	0.0472267
36	9.012	1045	92.1	0.0303257	4	0.238404	0.6	0.5	9.012	1044.9	4.2	0.0319428
37	9.3	1103	97.2	0.382388	5	3.08505	7.5	6.6	9.3	1102.8	4.6	0.37976

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.645	197	17.4	0.100922	4	0.643996	2.4	2	4.645	197.3	3.9	0.0936848
2	5.072	249	22	0.0502202	5	0.3939	1.5	1.2	5.072	249.8	5	0.0442523
3	5.379	303	26.7	0.0855725	5	0.66055	2.5	2.1	5.379	302	4.5	0.0828058
4	5.477	324	28.6	0.107356	3	0.59828	2.3	1.9	5.477	323.4	3.1	0.10894
5	5.51	331	29.2	0.16085	4	1.02058	3.8	3.2	5.51	331.6	3.7	0.154379
6	5.696	371	32.7	0.0605845	3	0.304531	1.1	1	5.696	370.8	3	0.0572846
7	6.017	443	39.1	0.331667	2	1.33127	5	4.2	6.017	442.9	2.3	0.328848
8	6.103	463	40.8	0.202794	2	0.830167	3.1	2.6	6.103	463.4	2.4	0.198353
9	6.357	522	46	0.417864	3	2.09551	7.9	6.6	6.357	522	2.8	0.420607
10	6.447	543	47.9	0.286446	2	1.18396	4.5	3.7	6.447	543.1	2.4	0.279257
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.498	555	48.9	0.0772971	2	0.273337	1	0.9	6.498	555	2	0.0777887
12	6.529	562	49.6	0.184987	2	0.674505	2.5	2.1	6.529	562.5	2	0.194962
13	6.574	570	50.3	0.26003	2	1.03364	3.9	3.2	6.574	569.9	2.2	0.262359
14	6.637	578	51	0.294992	3	1.59296	6	5	6.637	578.4	2.9	0.306396
15	6.755	593	52.3	0.121872	2	0.424198	1.6	1.3	6.755	593.2	1.9	0.124564
16	7.144	658	58	0.323137	2	1.10054	4.1	3.5	7.144	658.1	1.9	0.321428
17	7.272	681	60.1	0.0714035	5	0.568188	2.1	1.8	7.272	680.9	4.7	0.0686701
18	7.411	707	62.3	0.27095	3	1.2407	4.7	3.9	7.411	706.8	2.6	0.269081
19	7.529	730	64.4	0.165708	4	1.22834	4.6	3.9	7.529	729.2	4.2	0.163407
20	7.718	767	67.6	0.0849503	4	0.614042	2.3	1.9	7.718	767.3	4.1	0.0843763
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.795	782	69	0.196714	5	1.94258	7.3	6.1	7.795	781.4	5.5	0.200318
22	8.467	926	81.7	0.037985	10	0.651866	2.5	2	8.467	925.8	9.8	0.0376798
23	8.639	966	85.2	0.207703	13	4.61314	17.4	14.5	8.639	966.8	13.5	0.193087
24	8.778	994	87.7	0.0677805	4	0.312478	1.2	1	8.778	996.1	4.4	0.0398147
25	8.867	1012	89.2	0.0406689	12	0.831722	3.1	2.6	8.867	1010.6	11.9	0.039396
26	9.002	1039	91.6	0.0527534	4	0.378446	1.4	1.2	9.002	1039.2	4.1	0.0516652

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.3	0.0974725	9	1.4978	9	7.3	3.5	38.8	9.3	0.090781
2	4.55	178	15.7	0.157108	5	1.19214	7.1	5.8	4.55	178.4	4.9	0.137024
3	5.2	254	22.4	0.190479	5	1.5471	9.3	7.5	5.2	254.2	4.8	0.180679
4	5.85	396	34.9	0.371339	2	1.65441	9.9	8	5.85	396.6	2.4	0.383295
5	6.55	564	49.7	0.205409	5	1.58765	9.5	7.7	6.55	563.8	4.6	0.194484
6	6.85	602	53.1	0.0716109	7	0.80058	4.8	3.9	6.85	601.7	7.2	0.0628966
7	7.35	694	61.2	0.169721	5	1.41845	8.5	6.9	7.35	693.5	4.9	0.162026
8	8.15	855	75.4	0.0666127	11	1.20011	7.2	5.8	8.15	856.3	10.5	0.0642917
9	8.45	923	81.4	0.0986109	7	1.28996	7.7	6.3	8.45	923.5	7.4	0.0988943
10	8.65	966	85.2	0.0948983	6	1.00952	6	4.9	8.65	966.7	6.1	0.0927931
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.194	1074	94.7	0.0453401	17	1.06512	6.4	5.2	9.194	1078.1	17.1	0.0352052
12	9.3	1095	96.6	0.338222	4	2.44196	14.6	11.8	9.3	1095.2	4.2	0.329933

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.669	191	16.9	0.0565325	5	0.485405	1.8	1.5	4.669	190.1	5.1	0.053458
2	5.111	243	21.4	0.019293	3	0.137587	0.5	0.4	5.111	242	3.5	0.0224583
3	5.399	297	26.2	0.0582669	4	0.449532	1.6	1.4	5.399	296.4	4.4	0.057234
4	5.491	317	28	0.0990327	4	0.623441	2.2	2	5.491	317	3.6	0.096682
5	5.527	325	28.7	0.148539	4	0.969629	3.5	3.1	5.527	325.1	3.8	0.143001
6	5.651	352	31.1	0.0452709	4	0.33772	1.2	1.1	5.651	352.5	4.2	0.0458966
7	5.706	364	32.1	0.0521681	2	0.212878	0.8	0.7	5.706	363.7	2.4	0.0510168
8	6.008	433	38.2	0.277235	2	1.18328	4.3	3.7	6.008	433.6	2.4	0.282745
9	6.096	454	40.1	0.168018	3	0.728634	2.6	2.3	6.096	454	2.6	0.157773
10	6.344	513	45.3	0.408359	3	2.0589	7.4	6.5	6.344	512.8	2.8	0.413699
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.432	534	47.1	0.287878	2	1.19221	4.3	3.8	6.432	534.1	2.4	0.280826
12	6.483	546	48.2	0.0676015	2	0.282925	1	0.9	6.483	545.8	2.3	0.0685384
13	6.516	554	48.9	0.172525	2	0.641677	2.3	2	6.516	553.7	2	0.178984
14	6.546	561	49.5	0.247039	2	1.00195	3.6	3.2	6.546	561.3	2.3	0.251244
15	6.606	569	50.2	0.28639	3	1.63303	5.9	5.2	6.606	569.9	3.1	0.301187
16	6.734	585	51.6	0.123844	2	0.416736	1.5	1.3	6.734	585	1.9	0.124734
17	7.123	650	57.4	0.316533	2	1.05387	3.8	3.3	7.123	650.2	1.9	0.319174
18	7.274	678	59.8	0.0711401	7	0.787759	2.8	2.5	7.274	677.5	6.9	0.0648277
19	7.385	699	61.7	0.323769	2	1.24535	4.5	3.9	7.385	698.7	2.2	0.325238
20	7.513	725	64	0.197325	4	1.43457	5.2	4.5	7.513	725.2	4.2	0.191968
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.687	760	67.1	0.111008	3	0.660371	2.4	2.1	7.687	759.6	3.3	0.111352
22	7.786	780	68.8	0.177836	6	1.88167	6.8	6	7.786	780.3	6	0.177416
23	8.47	925	81.6	0.0430217	9	0.724235	2.6	2.3	8.47	923	9.3	0.0438962
24	8.643	963	85	0.233061	13	4.89539	17.7	15.5	8.643	963.6	13.1	0.211128
25	8.801	995	87.8	0.0775644	10	1.24108	4.5	3.9	8.801	995.8	10.3	0.0679614
26	8.905	1016	89.7	0.0743756	8	1.00387	3.6	3.2	8.905	1015.4	7.7	0.0735897
27	9.018	1039	91.7	0.0573789	4	0.436109	1.6	1.4	9.018	1038.5	4.4	0.0565459

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.0989561	10	1.6002	10	7.7	3.5	36.2	9.6	0.0938459
2	4.55	176	15.5	0.136311	4	0.888683	5.6	4.3	4.55	175.8	4.3	0.116223
3	5.2	253	22.3	0.182616	5	1.41771	8.9	6.8	5.2	253.2	4.6	0.172344
4	5.85	395	34.9	0.359407	2	1.58727	10	7.6	5.85	394.8	2.5	0.360617
5	6.55	560	49.5	0.230981	4	1.66469	10.4	8	6.55	559.6	4.3	0.219237
6	6.85	597	52.7	0.0761484	7	0.853412	5.4	4.1	6.85	597.1	7.2	0.0670704
7	7.35	690	61	0.212297	5	1.64218	10.3	7.9	7.35	689.1	4.6	0.200911
8	8.15	852	75.3	0.0628448	11	1.19401	7.5	5.7	8.15	849.9	11	0.0612778
9	8.45	918	81.1	0.101722	7	1.28425	8.1	6.1	8.45	917.7	7.1	0.102038
10	8.65	963	85.1	0.105609	6	1.06328	6.7	5.1	8.65	962.4	5.9	0.102159
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1097	96.9	0.620385	3	2.74831	17.2	13.2	9.3	1096.9	2.7	0.580051

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 124

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 11.800 4.550 7.600 5.200 9.600 5.850 9.800 6.550 9.300 6.850 5.000 7.350 9.600 8.150 7.400 8.450 7.500 8.650 5.800 9.300 16.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 5.800 4.550 2.200 4.613 1.300 4.954 0.500 5.049 1.800 5.200 2.900 5.276 0.800 5.376 1.100 5.470 1.000 5.504 3.300 5.627 1.100 5.689 1.500 5.850 3.500 6.014 3.000 6.107 1.900 6.368 5.000 6.461 2.500 6.510 1.100 6.550 2.300 6.613 1.900 6.676 3.500 6.803 0.900 6.850 0.400 7.180 2.400 7.350 3.700 7.445 2.600 7.587 3.400 7.766 1.500 7.866 4.500 8.150 3.000 8.450 1.100 8.514 6.500 8.650 12.100 8.789 0.500 8.903 1.300 9.012 0.600 9.300 7.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 37 Return to Lane Navigator
4.645 2.400 5.072 1.500 5.379 2.500 5.477 2.300 5.510 3.800 5.696 1.100 6.017 5.000 6.103 3.100 6.357 7.900 6.447 4.500 6.498 1.000 6.529 2.500 6.574 3.900 6.637 6.000 6.755 1.600 7.144 4.100 7.272 2.100 7.411 4.700 7.529 4.600 7.718 2.300 7.795 7.300 8.467 2.500 8.639 17.400 8.778 1.200 8.867 3.100 9.002 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 26 Return to Lane Navigator
3.500 9.000 4.550 7.100 5.200 9.300 5.850 9.900 6.550 9.500 6.850 4.800 7.350 8.500 8.150 7.200 8.450 7.700 8.650 6.000 9.194 6.400 9.300 14.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 12 Return to Lane Navigator
4.669 1.800 5.111 0.500 5.399 1.600 5.491 2.200 5.527 3.500 5.651 1.200 5.706 0.800 6.008 4.300 6.096 2.600 6.344 7.400 6.432 4.300 6.483 1.000 6.516 2.300 6.546 3.600 6.606 5.900 6.734 1.500 7.123 3.800 7.274 2.800 7.385 4.500 7.513 5.200 7.687 2.400 7.786 6.800 8.470 2.600 8.643 17.700 8.801 4.500 8.905 3.600 9.018 1.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 27 Return to Lane Navigator
3.500 10.000 4.550 5.600 5.200 8.900 5.850 10.000 6.550 10.400 6.850 5.400 7.350 10.300 8.150 7.500 8.450 8.100 8.650 6.700 9.300 17.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Coho Salmon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Coho Salmon ---- Go to Display Gel Lane Navigator.