Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
California Sheephead  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Semicossyphus pulcher
FDA Seafood List Common Name: California Sheephead
FDA Seafood List Market Name: Sheephead
Unique IEF Gel Code: 19970205gel08
Unique Web Code: clh01Srr
Tissue Aliquot Code: semipulc J96-002 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55769.50.072441940.5269535.64.14.5576.74.20.0706841
25.218523.20.11704240.9041919.67.15.2185.14.50.11435
35.8530037.50.092585950.8081158.56.35.85299.95.10.0885519
46.5543354.20.14477771.6912217.913.26.55431.57.10.135237
56.8546658.30.052706640.3855984.136.85466.44.30.0504642
67.3553466.80.1109840.840038.96.67.35534.24.40.107678
78.1565682.10.0853505101.5372216.2128.15654.210.40.0832971
88.4571589.50.17294661.8425119.514.48.45713.96.30.164324
98.6576495.60.12564540.9289879.87.38.65763.54.30.122105
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.197172.10.41527175.0611815.3104.19718.37.20.395226
24.566769.50.51022143.9558711.97.84.56676.24.40.51157
34.6979712.20.014884450.123560.40.24.697954.60.0152317
44.75310613.30.032117930.1530440.50.34.753106.52.70.0319737
54.96614017.50.038661250.33515810.74.966139.750.0379442
65.05915519.40.013783830.06614830.20.15.059155.12.90.0130035
75.24518623.30.062705250.4695321.40.95.245185.84.60.057547
85.3320225.30.32770831.802545.43.65.332012.80.36905
95.36220826.10.47231432.3153774.65.362207.62.70.482294
105.39421426.80.24479731.122293.42.25.394214.32.50.250984
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.47322928.70.02708530.1124350.30.25.473229.72.50.0250387
125.5925131.50.032373220.1217290.40.25.59251.51.90.0358986
135.67526733.50.43278931.940455.93.85.675267.32.60.418636
145.7728535.70.043550230.1747330.50.35.77284.82.50.0392603
155.8530037.60.59807643.7321911.37.45.85300.53.90.544866
165.94431839.80.32321742.161566.54.35.944318.64.10.298928
176.0934643.40.12465951.078393.32.16.09344.85.20.11756
186.158359450.065243530.32177310.66.158359.42.90.0626302
196.21637046.40.19234320.7447552.21.56.216370.12.10.195888
206.29938648.40.073210620.2720430.80.56.299385.72.20.0699058
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.33639349.20.36924541.9905663.96.336393.23.50.320292
226.39940550.80.22082451.701075.13.46.399405.14.90.195149
236.62144255.40.039402660.3564761.10.76.621443.86.40.0316128
246.86647058.90.017971720.07494270.20.16.866469.72.40.0176524
256.95248160.30.22028720.9194192.81.86.952481.12.40.215286
268.72278197.90.17488872.030466.148.722780.47.10.16204
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55739.10.040845660.3908442.94.5573.45.50.0400843
25.217221.50.048001280.716017.45.35.2171.98.20.0493585
35.8530237.70.10902350.9411379.775.85301.84.90.108732
46.5543754.60.14880571.7003917.512.66.55436.66.90.139698
56.8547258.90.059900150.4693724.83.56.854724.60.0571199
67.3553266.40.11314950.8909829.26.67.35532.24.50.111544
78.1565681.90.100458111.8188318.713.58.15655.510.80.0947819
88.4571789.50.15861471.8330118.913.68.45716.16.80.151657
98.6576595.50.12480550.9571449.87.18.65764.84.50.118988
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.237202.50.30638573.7630113.810.44.237227.30.290835
24.55718.90.44733443.4867512.89.74.55714.40.449268
34.7099712.10.016129270.1870890.70.54.70998.16.60.0160685
44.90612916.10.014934560.1468370.50.44.906129.55.60.0146882
55.217722.10.042125150.3695761.415.2176.44.90.04225
65.279192240.19012841.162944.33.25.2791924.30.151106
75.31619924.80.26100251.874726.95.25.316198.74.60.231535
85.35320625.70.10961720.4324431.61.25.353206.72.40.099655
95.43822227.70.011775790.136530.50.45.438224.48.80.00877331
105.56524630.70.016512640.1197940.40.35.5652474.40.0153234
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.6726633.20.23095141.73956.44.85.67265.94.10.239714
125.8530037.50.44989843.306612.19.25.85300.34.30.431211
135.93831639.50.29160862.451396.85.9383165.50.250969
146.06533942.30.13425871.45445.346.065337.16.60.123743
156.14835444.20.026219820.07132410.30.26.1483541.80.0219062
166.20836545.60.13497420.53740721.56.208365.42.30.133014
176.34639048.70.28676741.545055.74.36.346390.43.80.23077
186.41240250.20.080195330.3859311.41.16.412401.92.90.0742951
196.5542753.30.023274190.2997651.10.86.55425.98.50.0198942
206.83646858.40.035002540.27045110.76.836467.24.20.0359163
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.9548260.20.097588920.3764711.416.95481.62.10.100572
227.3553066.20.065777540.5041961.91.47.35529.84.40.0643758
238.1566082.40.036194780.5067421.91.48.15658.97.80.0367661
248.4571989.80.079971460.756932.82.18.45718.45.60.0763056
258.6576495.40.069300450.6279492.31.78.65762.65.20.0682087
268.69477496.60.080495750.7077612.628.694774.550.0797468
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.198212.60.47683675.6387315.19.74.19822.37.10.448631
24.55769.50.51797854.3165911.57.44.5576.14.70.512776
34.729104130.0427703180.9926532.71.74.72996.8180.0311142
44.92113416.70.024827340.1616170.40.34.921135.74.20.0218114
55.20817922.30.046481670.5160071.40.95.208176.66.50.0447577
65.26118923.60.13651820.1347490.40.25.261187.81.80.0429683
75.29919624.40.3210883.8049710.26.55.299196.67.70.28019
85.35220625.70.17077761.298033.52.25.352206.85.80.125514
95.45322528.10.022396350.1903850.50.35.453227.15.20.0205925
105.60825431.70.033046660.3350630.90.65.608255.85.90.0318966
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.71427434.20.3194442.340936.345.714274.34.10.318366
125.85830137.50.45146854.0027610.76.95.858300.34.90.459165
135.922313390.25803272.9999585.25.922313.46.70.254028
146.03433441.60.13499161.361493.62.36.034333.55.90.131205
156.10334743.30.037093720.1216240.30.26.103347.62.30.0304667
166.16735944.80.1241630.7223831.91.26.167359.33.10.130389
176.25737646.90.088672640.6497411.71.16.257377.23.90.0951998
186.338447.90.34891932.001165.33.46.3383.83.30.340188
196.33239048.60.14461930.6916721.81.26.3323902.90.134459
206.3839949.80.23906141.555234.22.76.38399.13.90.224493
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.61343954.70.045321930.2069560.60.46.613439.22.70.0437277
226.83446758.20.020240840.1400430.40.26.834467.93.80.0210372
236.93848059.90.21936820.9575762.61.66.938480.52.40.227934
248.70677496.50.17424982.276576.13.98.706773.97.80.165644
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558210.20.072994350.6000945.94.64.5582.44.60.0737427
25.217922.20.085850860.8893528.86.85.2179.65.80.0871537
35.8530037.20.14066651.1269711.28.65.85299.44.60.139414
46.5543654.10.12907961.418011410.86.55435.46.40.124818
56.8546958.20.057570650.4352594.33.36.85469.64.70.0520699
67.3553466.30.101750.8702868.66.67.355344.90.100594
78.1565981.80.0822865101.512491511.58.15658.410.30.0829941
88.4571588.70.17447772.1365821.216.28.45714.57.20.167065
98.6576094.30.12541551.11031118.48.65759.55.10.122864
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln