RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Bllue Marlin ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Makaira nigricans
FDA Seafood List Common Name: Blue Marlin
FDA Seafood List Market Name: Marlin
Unique IEF Gel Code: 19970212gel06
Unique Web Code: bmh01Srr
Tissue Aliquot Code: makanigr I96-001 #04 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	15	2	0.059952	4	0.427465	4.3	3.3	4.55	15.1	4.2	0.0571116
2	5.2	105	13.7	0.0928311	5	0.71728	7.2	5.5	5.2	105.1	4.6	0.0884151
3	5.85	220	28.8	0.2231	3	1.37434	13.7	10.5	5.85	220.1	3.5	0.222324
4	6.55	358	46.8	0.141395	7	1.683	16.8	12.9	6.55	357.2	7.3	0.130183
5	6.85	395	51.6	0.0440881	5	0.382031	3.8	2.9	6.85	396	5.3	0.0406443
6	7.35	461	60.3	0.0779494	5	0.64349	6.4	4.9	7.35	460.7	4.9	0.0741818
7	8.15	582	76.1	0.100524	8	1.38515	13.9	10.6	8.15	581.8	8	0.0972238
8	8.45	650	85	0.136342	7	1.67175	16.7	12.8	8.45	649.5	7	0.134506
9	8.65	701	91.6	0.105295	4	0.7574	7.6	5.8	8.65	700.9	4.2	0.102294
10	9.3	745	97.4	0.0758564	9	0.953258	9.5	7.3	9.3	742.6	8.6	0.062343

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.191	289	37.7	0.028373	7	0.378687	1.1	1	6.191	286.6	7.3	0.0291201
2	6.3	310	40.5	0.482155	3	2.33135	6.6	6.3	6.3	310.4	2.7	0.47867
3	6.404	330	43.1	0.170402	4	0.939937	2.7	2.6	6.404	329.4	3.8	0.139714
4	6.425	334	43.6	0.144266	3	0.705545	2	1.9	6.425	334.9	3.3	0.119532
5	6.524	353	46.1	0.136753	3	0.605816	1.7	1.6	6.524	352.7	2.6	0.130932
6	6.753	382	49.9	0.0327654	5	0.24245	0.7	0.7	6.753	380.3	5.3	0.0255974
7	6.891	399	52.1	0.0364373	4	0.230842	0.7	0.6	6.891	398.5	3.9	0.0336054
8	6.989	412	53.8	0.0406614	2	0.148042	0.4	0.4	6.989	412	2.1	0.0401074
9	7.124	430	56.1	0.245935	5	2.14132	6.1	5.8	7.124	429.9	5.1	0.237275
10	7.252	447	58.4	0.568402	3	3.15567	9	8.6	7.252	446.6	3	0.586547
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.327	457	59.7	0.336132	4	2.30729	6.6	6.3	7.327	457.8	4.1	0.320201
12	7.703	514	67.1	0.143413	13	1.87431	5.3	5.1	7.703	511.5	13.3	0.0795107
13	7.997	559	73	0.113095	23	8.45837	24	23	7.997	540.7	22.7	0.210505
14	8.127	579	75.6	0.172902	6	1.29572	3.7	3.5	8.127	580.4	5.7	0.128001
15	8.373	632	82.5	0.0445754	8	0.585952	1.7	1.6	8.373	632.5	8.2	0.0402645
16	8.469	654	85.4	0.053538	5	0.487742	1.4	1.3	8.469	655.4	5.2	0.0528903
17	8.516	666	86.9	0.0814344	4	0.595763	1.7	1.6	8.516	665.5	4.2	0.0799317
18	8.582	683	89.2	0.469832	3	2.60341	7.4	7.1	8.582	683.1	3.1	0.477605
19	8.798	711	92.8	0.0930677	23	4.43822	12.6	12.1	8.798	721.8	22.7	0.110514
20	9.265	744	97.1	0.169806	8	1.65203	4.7	4.5	9.265	742.5	7.9	0.118267

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	15	2	0.0572285	4	0.410124	3.9	3	4.55	15	4.1	0.0565657
2	5.2	113	14.7	0.100588	5	0.867024	8.2	6.4	5.2	112.8	5.2	0.0948001
3	5.85	227	29.6	0.198871	4	1.27841	12.1	9.5	5.85	227.2	3.7	0.196356
4	6.55	358	46.7	0.138826	8	1.67294	15.8	12.4	6.55	356.6	7.7	0.123326
5	6.85	392	51.1	0.0532085	5	0.454124	4.3	3.4	6.85	392.9	5.4	0.0474901
6	7.35	459	59.8	0.0707958	4	0.543317	5.1	4	7.35	459.1	4.4	0.0697284
7	8.15	583	76	0.11648	8	1.50882	14.2	11.2	8.15	582.2	7.6	0.112405
8	8.45	648	84.5	0.111158	8	1.55563	14.7	11.5	8.45	647.6	8.3	0.106204
9	8.65	700	91.3	0.101937	4	0.7741	7.3	5.7	8.65	699.5	4.4	0.0991945
10	9.3	748	97.5	0.0978508	12	1.52826	14.4	11.3	9.3	743.1	12	0.0717371

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.0193013	6	0.196393	0.8	0.7	4.55	20.1	6.2	0.0178013
2	5.2	116	15	0.0253118	7	0.296612	1.2	1.1	5.2	115	6.8	0.0247472
3	5.85	234	30.3	0.0666313	6	0.651309	2.6	2.3	5.85	233.4	5.8	0.063796
4	6.223	299	38.7	0.0142985	7	0.166974	0.7	0.6	6.223	297	6.9	0.0135568
5	6.332	318	41.1	0.325086	3	1.71569	6.8	6.1	6.332	318	3.1	0.312606
6	6.435	336	43.5	0.149146	8	1.99354	7.9	7.1	6.435	337.8	7.8	0.145007
7	6.55	356	46.1	0.128506	6	1.16615	4.6	4.2	6.55	355.9	6	0.109291
8	6.75	382	49.4	0.0145415	3	0.0700686	0.3	0.2	6.75	381.3	3.4	0.0117278
9	6.85	395	51.1	0.0422027	5	0.389214	1.5	1.4	6.85	396.2	4.9	0.0443899
10	6.968	411	53.2	0.0215822	2	0.0634473	0.3	0.2	6.968	411.2	1.7	0.0212507
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.094	428	55.4	0.153014	5	1.38002	5.5	4.9	7.094	428.5	5.2	0.14922
12	7.219	445	57.6	0.543362	3	2.69535	10.7	9.6	7.219	444.6	2.7	0.558944
13	7.293	455	58.9	0.259405	4	1.99407	7.9	7.1	7.293	456.5	4.5	0.251211
14	7.691	514	66.5	0.115618	21	4.17259	16.5	14.9	7.691	518.7	21.2	0.110957
15	8.024	564	73	0.063957	5	0.442479	1.8	1.6	8.024	564.6	5	0.0497749
16	8.15	583	75.4	0.157411	7	1.91052	7.6	6.8	8.15	582.3	6.9	0.157305
17	8.352	630	81.5	0.0242125	5	0.191299	0.8	0.7	8.352	629.2	4.6	0.0235391
18	8.451	653	84.5	0.0557403	5	0.411021	1.6	1.5	8.451	652.9	4.6	0.050233
19	8.474	659	85.3	0.0815878	4	0.509389	2	1.8	8.474	658.8	4.1	0.0709603
20	8.493	664	85.9	0.0685611	3	0.261865	1	0.9	8.493	663.8	2.7	0.0546509
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.558	681	88.1	0.367172	3	2.13777	8.5	7.6	8.558	681.3	3.2	0.37184
22	8.65	705	91.2	0.0617354	8	0.787983	3.1	2.8	8.65	705.9	8.4	0.0531346
23	9.228	748	96.8	0.103522	9	1.66913	6.6	6	9.228	746.5	9.2	0.102852

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	6.196	290	37.8	0.0273939	6	0.32422	0.8	0.8	6.196	289.5	6.4	0.0287959
2	6.31	311	40.5	0.496429	3	2.50301	6.4	6.1	6.31	310.8	2.9	0.479515
3	6.408	329	42.8	0.178144	3	0.757336	1.9	1.8	6.408	327.7	3.2	0.132117
4	6.425	332	43.2	0.171147	4	0.99968	2.5	2.4	6.425	332.6	3.7	0.153742
5	6.517	349	45.4	0.143847	3	0.64981	1.6	1.6	6.517	349	2.7	0.135317
6	6.721	375	48.8	0.0303542	5	0.198492	0.5	0.5	6.721	373.5	4.6	0.0244468
7	6.865	392	51	0.0322056	3	0.187188	0.5	0.5	6.865	391.5	3.4	0.031007
8	6.956	404	52.6	0.0354771	2	0.122341	0.3	0.3	6.956	404.1	1.9	0.0355285
9	7.084	421	54.8	0.227705	5	2.23906	5.7	5.4	7.084	421.3	5.4	0.231882
10	7.212	438	57	0.579054	3	3.05535	7.8	7.4	7.212	437.8	3	0.579443
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.287	448	58.3	0.342984	4	2.20544	5.6	5.4	7.287	449.2	4	0.314128
12	7.687	507	66	0.158628	24	6.45203	16.4	15.7	7.687	513.4	23.9	0.152528
13	7.987	552	71.9	0.208731	16	6.30569	16	15.3	7.987	541.3	16	0.222305
14	8.133	574	74.7	0.210933	6	2.04806	5.2	5	8.133	575	6.4	0.180435
15	8.364	627	81.6	0.0329914	10	0.541657	1.4	1.3	8.364	627.9	9.6	0.0317478
16	8.449	647	84.2	0.0572857	6	0.59451	1.5	1.4	8.449	649.7	6.1	0.0547791
17	8.495	659	85.8	0.100103	3	0.509298	1.3	1.2	8.495	659.3	3.1	0.0926732
18	8.569	678	88.3	0.54347	3	2.8418	7.2	6.9	8.569	677.6	2.9	0.543573
19	8.742	706	91.9	0.0970691	24	5.65217	14.3	13.8	8.742	723.5	24	0.132873
20	9.226	743	96.7	0.197283	6	1.20902	3.1	2.9	9.226	742.2	6	0.113042

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.1	0.0519321	4	0.41002	4.2	3.2	4.55	24.3	4.5	0.0515746
2	5.2	119	15.5	0.0828199	6	0.913134	9.3	7.1	5.2	117.3	6.4	0.0806841
3	5.85	230	29.9	0.216201	4	1.43408	14.6	11.2	5.85	229.6	3.8	0.213488
4	6.55	365	47.4	0.155432	7	1.71064	17.4	13.4	6.55	363.6	6.6	0.145733
5	6.85	396	51.4	0.0554616	4	0.384874	3.9	3	6.85	395.9	4	0.0541835
6	7.35	461	59.9	0.0893644	4	0.687553	7	5.4	7.35	460.7	4.5	0.0870204
7	8.15	581	75.5	0.112122	7	1.4081	14.4	11	8.15	581	7.4	0.106918
8	8.45	653	84.8	0.139307	7	1.61154	16.4	12.6	8.45	651.7	6.6	0.136899
9	8.65	704	91.4	0.0920958	5	0.823952	8.4	6.4	8.65	703.6	5.2	0.0897652
10	9.3	755	98.1	0.0644393	4	0.42379	4.3	3.3	9.3	754.4	4	0.0592401

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 93

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.300 5.200 7.200 5.850 13.700 6.550 16.800 6.850 3.800 7.350 6.400 8.150 13.900 8.450 16.700 8.650 7.600 9.300 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
6.191 1.100 6.300 6.600 6.404 2.700 6.425 2.000 6.524 1.700 6.753 0.700 6.891 0.700 6.989 0.400 7.124 6.100 7.252 9.000 7.327 6.600 7.703 5.300 7.997 24.000 8.127 3.700 8.373 1.700 8.469 1.400 8.516 1.700 8.582 7.400 8.798 12.600 9.265 4.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 3.900 5.200 8.200 5.850 12.100 6.550 15.800 6.850 4.300 7.350 5.100 8.150 14.200 8.450 14.700 8.650 7.300 9.300 14.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 0.800 5.200 1.200 5.850 2.600 6.223 0.700 6.332 6.800 6.435 7.900 6.550 4.600 6.750 0.300 6.850 1.500 6.968 0.300 7.094 5.500 7.219 10.700 7.293 7.900 7.691 16.500 8.024 1.800 8.150 7.600 8.352 0.800 8.451 1.600 8.474 2.000 8.493 1.000 8.558 8.500 8.650 3.100 9.228 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
6.196 0.800 6.310 6.400 6.408 1.900 6.425 2.500 6.517 1.600 6.721 0.500 6.865 0.500 6.956 0.300 7.084 5.700 7.212 7.800 7.287 5.600 7.687 16.400 7.987 16.000 8.133 5.200 8.364 1.400 8.449 1.500 8.495 1.300 8.569 7.200 8.742 14.300 9.226 3.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 4.200 5.200 9.300 5.850 14.600 6.550 17.400 6.850 3.900 7.350 7.000 8.150 14.400 8.450 16.400 8.650 8.400 9.300 4.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Bllue Marlin ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Bllue Marlin ---- Go to Display Gel Lane Navigator.