Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Albacore  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Thunnus alalunga
FDA Seafood List Common Name: Albacore
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970130gel06
Unique Web Code: alh03Srr
Tissue Aliquot Code: thunalal E96-002 #08a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55243.20.12839440.8809796.44.84.5524.83.90.126304
25.211615.20.19572941.4379710.47.85.2116.14.30.188268
35.85213280.22344231.263319.26.95.85213.13.20.21968
46.5534445.20.17703282.3018816.712.56.55342.67.80.16634
56.8537549.30.096024640.7153545.23.96.85374.54.40.0913344
67.3544458.30.17468741.315289.67.17.35443.44.30.171364
78.1556474.10.18294972.197961611.98.15563.46.90.179871
88.4563483.30.16100882.1872715.911.98.45632.37.90.155904
98.6568289.60.11612450.9412566.85.18.656824.60.115907
109.374898.30.09106830.5310163.92.99.37483.30.0905202
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.39514118.50.033049620.1375540.40.35.395140.62.30.0332201
25.57316822.10.035088430.1652650.50.45.573167.62.70.0339538
35.771198260.015890850.1164720.30.35.771198.44.70.0139953
46.0324331.90.021173320.07637660.20.26.03243.620.0212074
56.19327335.90.044721220.1560170.50.46.193273.220.0443516
66.43331741.70.13173251.3770143.46.433318.75.30.147503
76.487327430.5102632.73797.96.96.4873273.40.450627
86.59434345.10.50187843.071618.97.76.594342.33.60.487863
96.78736347.70.23267641.476164.33.76.787363.33.70.226782
106.83536848.40.13262120.3722581.10.96.835368.920.103326
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.9137849.70.073067720.266970.80.76.91377.920.0752639
127.0339551.90.11599240.7690732.21.97.03394.24.10.105224
137.142411540.062748590.9695422.82.47.142411.88.50.0641608
147.22742355.60.14532841.0241932.67.227424.53.80.151633
157.28343156.60.45550242.958298.67.47.283431.23.90.433326
167.36744358.20.10988761.535874.43.87.367439.95.90.14718
177.46145760.10.23773731.147053.32.97.461457.32.60.244723
187.57547462.30.50196932.806788.177.575473.53.10.516942
198.37961380.60.0872208131.996455.858.379604.313.20.0855901
208.51564684.90.42323443.263839.48.28.515645.94.40.415043
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.72668890.40.185764185.8088116.814.58.726681.3180.182074
229.3274798.20.24285762.350026.85.99.32746.65.50.240355
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55172.20.051190440.3427774.134.5516.63.70.0521803
25.2106140.075002450.7026828.56.15.2106.35.10.0770712
35.8520627.10.091694940.65956185.75.85206.240.0920132
46.5533243.70.13046861.2881615.511.16.55331.56.30.115712
56.8536347.80.041335540.2996743.62.66.85362.94.30.0395939
67.3543657.40.072222640.4879795.94.27.35436.340.0688105
78.15554730.1291161.3723616.611.88.15553.86.10.127311
88.4562482.20.13341761.328521611.58.456245.70.132364
98.6567889.30.10072381.1609914108.65675.480.082089
109.374197.60.054563370.6489517.85.69.3740.37.30.050053
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.552330.07561440.5009351.51.24.5522.63.70.0759737
25.210814.20.077387550.6610511.91.65.2107.850.0749111
35.35313217.40.010587170.1204450.40.35.353133.36.80.0099412
45.55716421.60.02154950.184840.50.55.557162.14.90.0211113
55.767197260.013902820.05034340.10.15.767196.620.0141281
65.8521027.70.088711140.69434221.75.85209.14.30.0913179
76.07324932.80.020448830.1226330.40.36.073248.73.40.0201161
86.15926434.80.023072440.1426790.40.46.159263.13.50.0229429
96.23927836.70.035152750.2935190.90.76.239278.45.30.0313708
106.43931341.30.064248640.5170491.51.36.439315.13.70.079854
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.50832542.90.32941663.39429.98.46.508326.46.40.298444
126.6234044.90.40025842.719567.96.86.62339.84.10.378098
136.83236347.90.16607451.400324.13.56.832362.950.158444
146.87236848.50.11337120.34866710.96.872369.42.40.0818363
156.94437849.90.041375120.1651030.50.46.944378.52.20.0429853
167.07439652.20.10109740.67336221.77.074395.83.90.0985533
177.167409540.077538560.9065152.62.37.167409.56.30.0811357
187.30442856.50.51822243.5535910.48.87.304427.73.90.517897
197.39644258.30.20668462.076156.15.27.396441.95.80.201753
207.50446060.70.22631531.085613.22.77.504460.22.70.227153
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.59947662.80.47732832.868538.47.17.599476.43.30.489935
228.1556874.90.0660574100.9939852.92.58.15563.410.10.0556735
238.35961681.30.032034850.2634950.80.78.359616.24.90.0304745
248.45637840.060285570.5165261.51.38.456396.70.0433956
258.564785.40.41876242.39464768.5647.13.70.366303
268.72768590.40.206231185.9594417.414.88.727680.9180.186795
279.3274498.20.24542841.69564.94.29.327443.90.247005
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.38413718.10.025853660.2606440.90.85.384138.45.90.0249267
25.60617222.70.020951570.2612210.90.85.606171.87.20.0203661
35.79620226.60.015002350.12780.50.45.796201.55.30.0135357
46.06924932.80.0438985140.9700523.43.16.069242.613.60.0402602
56.14326234.60.028183120.04328830.20.16.143262.21.80.0132862
66.21127436.10.041207240.2668060.90.86.211274.34.20.0354254
76.48432242.50.28958193.9412613.912.56.484324.69.10.245543
86.614341450.36172932.113847.56.76.614340.53.50.345133
96.684349460.014157610006.68434610
106.82436548.20.19897441.4058954.56.824365.34.10.192987
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.88837349.20.057715620.305961.116.888371.620.0858236
126.94138050.10.055649820.189490.70.66.941380.21.90.0559074
137.07139752.40.12574140.7620042.72.47.071396.63.50.121584
147.162409540.079722150.6804552.42.27.162410.24.80.0792317
157.29242656.20.54596543.6521412.911.67.292426.23.80.547124
167.37143757.70.18235461.886686.767.371438.25.80.18485
177.48845660.20.23390931.269314.547.488455.930.238559
187.59947462.50.47573143.0448110.79.67.5994743.50.485854
198.371614810.041505960.4425011.61.48.371615.15.90.0423661
208.52464985.60.39096842.692719.58.58.524648.63.90.386148
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.8269591.70.0911383152.381378.47.58.82699.714.90.090434
229.374498.20.19835351.660435.95.39.3743.24.70.197635
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55243.20.11746440.8119876.14.54.5524.640.11364
25.210814.20.19585441.4566410.98.15.2107.94.40.188394
35.8521127.80.21348631.285759.67.15.85210.73.40.211688
46.5533544.20.17329782.3347517.5136.55333.98.10.162196
56.8537148.90.10966640.8376186.34.76.853714.50.106073
67.3543357.10.21149941.6387312.39.17.35432.94.50.206244
78.1555973.70.16531772.0502815.311.48.15559.170.164343
88.4562782.70.0979446101.73904139.78.45628.49.80.100502
98.6567889.40.11145950.8884186.64.98.65677.84.60.109037
109.374698.40.044606440.323522.41.89.3745.340.0453403
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 101
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln