RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus morhua
FDA Seafood List Common Name: Atlantic Cod
FDA Seafood List Market Name: Cod
Unique IEF Gel Code: 19960122gel01
Unique Web Code: ach04Srr
Tissue Aliquot Code: gadumorh H94-002 #06a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	42	5.4	0.0239746	5	0.201705	14.4	8.4	4.55	41.1	4.7	0.0239643
2	5.2	145	18.6	0.014031	5	0.134528	9.6	5.6	5.2	144.8	5.3	0.0144475
3	5.85	252	32.3	0.0101403	4	0.0628468	4.5	2.6	5.85	252.9	3.7	0.0095526
4	6.55	373	47.9	0.0103991	9	0.136097	9.7	5.7	6.55	370.3	8.5	0.0089831
5	7.35	460	59.1	0.0139704	3	0.0810244	5.8	3.4	7.35	459.7	3.5	0.0130724
6	8.15	587	75.4	0.0176612	6	0.183328	13.1	7.7	8.15	586.5	6.1	0.0169708
7	8.45	649	83.3	0.0241405	7	0.28282	20.2	11.8	8.45	648.7	7.1	0.0225675
8	8.65	697	89.5	0.0199744	5	0.179677	12.8	7.5	8.65	696.9	5.2	0.0196651
9	9.3	754	96.8	0.0200073	4	0.136491	9.8	5.7	9.3	754.3	3.7	0.0208775

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.428	20	2.6	0.0049402	3	0.0187002	0.3	0.2	4.428	17.1	3.1	0.003416
2	4.56	40	5.2	0.0533077	12	1.10249	17.6	10.7	4.56	38.3	11.9	0.05221
3	5.134	127	16.4	0.0061278	4	0.0421969	0.7	0.4	5.134	125.6	4.2	0.0056575
4	5.18	134	17.3	0.0075545	3	0.0359301	0.6	0.4	5.18	134.5	2.7	0.0073766
5	5.268	149	19.2	0.208833	3	0.913081	14.6	8.9	5.268	149.2	2.8	0.183889
6	5.331	160	20.6	0.0331218	15	0.76634	12.2	7.5	5.331	160.6	15.5	0.0279359
7	5.394	171	22	0.11003	3	0.391143	6.2	3.8	5.394	170.4	2.5	0.0872244
8	5.559	200	25.8	0.0080089	4	0.044721	0.7	0.4	5.559	199	4	0.0063111
9	5.679	221	28.5	0.0096651	10	0.150146	2.4	1.5	5.679	222.4	9.7	0.0087491
10	5.742	232	29.9	0.0089588	3	0.0335944	0.5	0.3	5.742	233.1	3.2	0.0059408
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.821	246	31.7	0.169626	3	0.832777	13.3	8.1	5.821	246.2	2.8	0.164883
12	5.903	260	33.5	0.0670983	7	0.698249	11.2	6.8	5.903	260.3	7.4	0.0531399
13	5.974	272	35.1	0.096399	2	0.32016	5.1	3.1	5.974	272.4	2.1	0.086777
14	6.104	294	37.9	0.0301979	5	0.235743	3.8	2.3	6.104	292.2	4.9	0.0269486
15	6.134	299	38.5	0.0209487	4	0.117174	1.9	1.1	6.134	300.7	3.7	0.0179557
16	6.187	308	39.7	0.0656811	2	0.26189	4.2	2.6	6.187	307.9	2.4	0.0618381
17	6.281	324	41.8	0.012733	2	0.0457623	0.7	0.4	6.281	324.2	2.1	0.012077
18	6.382	341	43.9	0.0123298	3	0.059083	0.9	0.6	6.382	340.8	2.9	0.0115281
19	6.441	351	45.2	0.024562	5	0.19173	3.1	1.9	6.441	350.4	4.9	0.0219835

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	35	4.5	0.0264148	4	0.173851	13.2	6.8	4.55	35.5	4	0.0245833
2	5.2	129	16.6	0.0144967	4	0.0905098	6.9	3.6	5.2	129.8	3.7	0.0139626
3	5.839	248	31.9	0.0059075	6	0.0557455	4.2	2.2	5.839	248.4	6.1	0.0051476
4	6.55	366	47	0.0110443	7	0.100713	7.6	4	6.55	363	6.8	0.0083418
5	7.35	462	59.4	0.0092692	5	0.0692401	5.3	2.7	7.35	461.8	4.9	0.0079372
6	8.15	579	74.4	0.0187223	7	0.207561	15.7	8.1	8.15	579.7	6.5	0.0179882
7	8.45	641	82.4	0.0214159	8	0.275458	20.9	10.8	8.45	639.3	7.7	0.0200917
8	8.65	693	89.1	0.021344	5	0.170424	12.9	6.7	8.65	692.8	4.7	0.0205373
9	9.3	751	96.5	0.0261481	4	0.174981	13.3	6.9	9.3	751	3.9	0.0254363

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	28	3.6	0.0128095	2	0.024128	0.5	0.3	4.55	27.3	2.1	0.006489
2	4.665	46	5.9	0.0345169	16	0.92317	19.7	12.5	4.665	53.4	15.5	0.0335384
3	5.2	130	16.7	0.0140632	5	0.100712	2.2	1.4	5.2	129	4.5	0.0126122
4	5.276	144	18.5	0.165324	2	0.697963	14.9	9.5	5.276	143.8	2.5	0.158195
5	5.352	158	20.3	0.0180595	11	0.335151	7.2	4.6	5.352	155.1	10.9	0.0173852
6	5.4	167	21.5	0.0614446	4	0.356363	7.6	4.8	5.4	167	3.6	0.0556824
7	5.881	255	32.8	0.0877418	3	0.495039	10.6	6.7	5.881	254.6	3.2	0.0872495
8	5.988	272	35	0.0245057	6	0.227392	4.9	3.1	5.988	273.2	5.6	0.0229578
9	6.081	287	36.9	0.0397217	4	0.23498	5	3.2	6.081	286.9	3.7	0.0356251
10	6.181	303	39	0.0230534	5	0.178484	3.8	2.4	6.181	302.9	4.5	0.0222672
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.256	315	40.5	0.0361745	4	0.250029	5.3	3.4	6.256	313.7	4.1	0.0340325
12	6.338	328	42.2	0.0030515	2	0.01364	0.3	0.2	6.338	327.9	1.9	0.0040472
13	6.488	352	45.3	0.0201163	9	0.265767	5.7	3.6	6.488	351.6	8.9	0.0168075
14	6.55	362	46.6	0.0098882	4	0.0356014	0.8	0.5	6.55	363.9	3.7	0.0053566
15	7.35	457	58.8	0.0138335	7	0.161065	3.4	2.2	7.35	457.6	7.2	0.0125775
16	8.45	646	83.1	0.0150519	6	0.157628	3.4	2.1	8.45	644.5	6.3	0.0141389
17	8.65	696	89.6	0.0176298	5	0.141518	3	1.9	8.65	694.6	5.1	0.0156798
18	9.3	749	96.4	0.0139706	3	0.0844672	1.8	1.1	9.3	749.8	3.4	0.0140532

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.474	22	2.8	0.0083738	1	0	0	0	4.474	18	1	0
2	4.578	37	4.8	0.0637194	13	1.34016	21.6	12.3	4.578	41.3	13.4	0.0563316
3	5.213	130	16.8	0.0074199	4	0.0520565	0.8	0.5	5.213	129.2	4	0.0074335
4	5.304	147	19	0.155005	4	1.08262	17.5	10	5.304	146.4	4.2	0.145389
5	5.379	161	20.8	0.0173037	10	0.294317	4.7	2.7	5.379	160.3	10.2	0.0162143
6	5.443	173	22.4	0.0764446	4	0.500757	8.1	4.6	5.443	172	3.9	0.0730611
7	5.588	200	25.8	0.0074121	9	0.106633	1.7	1	5.588	198.3	8.9	0.006742
8	5.7	221	28.6	0.0099738	10	0.155157	2.5	1.4	5.7	222.4	10.3	0.008501
9	5.839	247	31.9	0.0887121	5	0.699718	11.3	6.4	5.839	246.6	4.6	0.0862612
10	5.958	266	34.4	0.0286965	4	0.212437	3.4	2	5.958	265.4	4.5	0.0266865
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.046	280	36.2	0.0531633	5	0.459932	7.4	4.2	6.046	277.9	5.3	0.0493599
12	6.154	297	38.4	0.0255349	6	0.260282	4.2	2.4	6.154	297	5.9	0.0247862
13	6.243	311	40.2	0.0268883	3	0.145209	2.3	1.3	6.243	310.9	3	0.0270226
14	6.338	326	42.1	0.011097	4	0.0680601	1.1	0.6	6.338	325	3.6	0.0105319
15	6.407	337	43.5	0.0150817	7	0.189376	3.1	1.7	6.407	338.6	6.7	0.0159696
16	6.477	348	45	0.0336582	4	0.221483	3.6	2	6.477	347.5	4.5	0.0278908
17	6.562	361	46.6	0.0102098	7	0.165461	2.7	1.5	6.562	356.5	7.2	0.0128954
18	7.419	468	60.5	0.009008	4	0.0441699	0.7	0.4	7.419	468	4.1	0.0060432
19	7.582	493	63.7	0.0135081	10	0.204478	3.3	1.9	7.582	491.9	9.6	0.012

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	40	5.2	0.0202322	4	0.128855	13.1	6.5	4.55	39.7	3.6	0.0202998
2	5.2	127	16.4	0.0028691	13	0.05462	5.5	2.8	5.2	129.6	12.7	0.0024358
3	6.55	359	46.3	0.0092147	8	0.113997	11.6	5.8	6.55	357.6	8.4	0.0076818
4	7.35	460	59.4	0.0082324	4	0.0552999	5.6	2.8	7.35	459.1	4.2	0.0074228
5	8.15	582	75.1	0.0154779	7	0.171714	17.4	8.7	8.15	581.4	6.9	0.0141191
6	8.45	645	83.2	0.0188052	7	0.235498	23.9	11.9	8.45	643.8	7.3	0.0183162
7	8.65	698	90.1	0.0181318	4	0.128089	13	6.5	8.65	696.7	3.9	0.0183481
8	9.3	753	97.2	0.013335	4	0.0967561	9.8	4.9	9.3	753.4	4.1	0.013215

PeakCounter: 8

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 82

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 14.400 5.200 9.600 5.850 4.500 6.550 9.700 7.350 5.800 8.150 13.100 8.450 20.200 8.650 12.800 9.300 9.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.428 0.300 4.560 17.600 5.134 0.700 5.180 0.600 5.268 14.600 5.331 12.200 5.394 6.200 5.559 0.700 5.679 2.400 5.742 0.500 5.821 13.300 5.903 11.200 5.974 5.100 6.104 3.800 6.134 1.900 6.187 4.200 6.281 0.700 6.382 0.900 6.441 3.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 19 Return to Lane Navigator
4.550 13.200 5.200 6.900 5.839 4.200 6.550 7.600 7.350 5.300 8.150 15.700 8.450 20.900 8.650 12.900 9.300 13.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 0.500 4.665 19.700 5.200 2.200 5.276 14.900 5.352 7.200 5.400 7.600 5.881 10.600 5.988 4.900 6.081 5.000 6.181 3.800 6.256 5.300 6.338 0.300 6.488 5.700 6.550 0.800 7.350 3.400 8.450 3.400 8.650 3.000 9.300 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 18 Return to Lane Navigator
4.474 0 4.578 21.600 5.213 0.800 5.304 17.500 5.379 4.700 5.443 8.100 5.588 1.700 5.700 2.500 5.839 11.300 5.958 3.400 6.046 7.400 6.154 4.200 6.243 2.300 6.338 1.100 6.407 3.100 6.477 3.600 6.562 2.700 7.419 0.700 7.582 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 19 Return to Lane Navigator
4.550 13.100 5.200 5.500 6.550 11.600 7.350 5.600 8.150 17.400 8.450 23.900 8.650 13.000 9.300 9.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 8 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.