Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Barracuda  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Sphyraena argentea
FDA Seafood List Common Name: Pacific Barracuda
FDA Seafood List Market Name: Barracuda
Unique IEF Gel Code: 19971030gel05
Unique Web Code: pbh01Srr
Tissue Aliquot Code: sphyarge J97-002 #09m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.553240.06617670.7834528.974.5534.46.90.064429
25.2121150.084021760.8653379.87.75.2121.36.10.0803296
35.8523028.60.12814551.0367811.79.35.85229.94.70.125383
46.5539348.80.095712381.2998214.711.66.55391.480.0914585
56.8543053.40.042424770.4629885.24.16.85431.26.80.0382171
67.3550162.20.099198540.7486688.56.77.35501.24.40.0964183
78.1562978.10.0818983101.3372215.111.98.15629.710.10.0743559
88.4569686.50.11185771.4292216.212.88.45694.27.40.109296
98.6574492.40.08094150.6261167.15.68.65743.64.60.0775453
109.379298.40.041163440.253762.92.39.3791.93.60.0396012
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.4572430.16223141.209085.53.84.45723.94.20.163327
24.861769.40.012135960.1489710.70.54.861746.30.0132886
35.37915018.60.031519130.1588130.70.55.379150.43.10.0290123
45.53217621.90.010318750.08545150.40.35.532176.44.90.0097735
55.74421226.30.010113450.08560710.40.35.744212.84.80.0100012
65.89324029.80.008403130.04281470.20.15.893240.13.10.0078443
75.96125631.80.023737330.123990.60.45.961255.53.20.0218013
85.99526432.80.009758330.04857030.20.25.995263.92.90.0093802
96.071282350.13431730.65936632.16.071281.92.90.129871
106.19531138.60.39746842.9029513.29.26.195311.54.30.381264
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.31834042.20.24122141.7549485.66.318339.34.20.234383
126.39535844.50.16195272.027979.26.46.395357.76.90.165858
136.46737546.60.1218981.67447.65.36.467376.27.50.12532
146.63440450.20.022606720.07679270.30.26.634403.82.10.0209131
156.74941751.80.1273830.66077432.16.749416.13.10.1201
166.79342252.40.051794650.4237621.91.36.793423.750.0482234
176.86743153.50.2865631.397436.44.46.867430.62.80.286603
186.93744154.80.015206830.09514280.40.36.9374433.40.0159031
197.16847458.90.47723843.5602816.211.37.168473.94.10.490045
207.33649861.90.12029571.246445.747.336498.870.100316
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.5371488.70.032753450.2928551.30.98.53713.75.10.0323662
228.60373190.80.13928661.600127.35.18.603732.46.20.144464
239.065773960.15119651.3219564.29.065772.250.147731
249.33479398.50.065731430.3547871.61.19.334792.530.0673601
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.554050.052241660.5524677.15.64.5541.26.40.048825
25.211814.70.055440670.6698848.66.75.2117.76.80.0556422
35.8523028.60.087212960.92959611.99.35.85228.66.50.0809412
46.5539649.20.1164461.1635714.911.76.55394.55.90.110746
56.85427530.040858260.4047475.24.16.85427.660.038173
67.35499620.081270640.5894167.55.97.35498.34.30.0770067
78.1563078.30.074287111.2360115.812.48.15631.110.50.0662028
88.4569586.30.10235171.237515.812.48.4569470.0991265
98.6574091.90.075026750.5767737.45.88.65739.64.50.0716435
109.3789980.063218540.4517995.84.59.3788.84.30.0599355
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55374.60.088405540.6286133.12.54.5536.740.089615
24.636496.10.05062740.3691631.81.54.63649.84.50.0464825
34.86481100.010313560.09841550.50.44.86481.45.80.0095268
45.01410212.60.005724550.04736280.20.25.014102.64.60.0058104
55.212815.80.041651370.4718482.31.95.2127.66.80.0389062
65.39616019.80.012864390.1628170.80.75.396159.79.40.0097905
75.56218723.10.005415250.0574350.30.25.562186.95.20.0061968
85.8523428.90.079499830.4826552.41.95.85233.33.40.0799481
95.97226332.50.014798950.1075160.50.45.972261.850.0121466
106.10329436.30.076675430.3620751.81.56.103293.82.80.0725567
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.23324400.34316842.1807810.78.86.23324.33.80.327901
126.33534943.10.17624131.078175.34.46.335349.13.40.176635
136.40236545.10.11503351.044715.14.26.402364.84.90.120359
146.44937646.50.048382540.19795610.86.449375.73.90.0284437
156.48738547.60.050565390.8053793.93.36.4873859.20.0496288
166.64641050.70.007531720.01721880.10.16.646410.41.60.0061482
176.73241951.80.090216720.2627281.31.16.732419.11.90.0765759
186.79942652.70.066835960.7289743.62.96.799426.46.30.0656888
196.86143353.50.29929720.9418664.63.86.861433.31.90.277092
206.94445550.013732430.06787030.30.36.94444.930.012813
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.17248059.30.43369242.7822613.611.27.172480.33.60.441371
227.3550762.70.14457741.044035.14.27.355074.40.133515
238.1563578.50.0553134141.714548.46.98.15640.914.10.0687729
248.4569786.20.038326150.3165781.51.38.45696.84.70.037747
258.53371688.50.025912330.1291410.60.58.533715.930.0244059
268.59973190.40.19621761.778058.77.28.599732.65.70.176882
279.02177195.30.19307682.2927411.29.39.021769.17.60.170392
289.28379197.80.051102930.2817021.41.19.2837913.20.0497871
PeakCounter: 28
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.51293.60.1478851.188245.94.64.5129.24.50.148336
24.857779.60.012664960.1172320.60.54.85775.85.60.0118101
35.40615819.60.026553230.1527420.80.65.406158.63.30.0257417
45.56718422.90.016266840.1235020.60.55.5671834.20.0164302
55.75121426.60.007565330.05368130.30.25.751213.63.40.0089491
65.95125431.60.021858430.1198050.60.55.951253.93.20.0213115
75.98926332.70.008583740.05957810.30.25.989263.73.70.0091711
86.08228535.40.11692830.572612.82.26.082284.42.80.11374
96.204314390.38924142.628491310.26.204314.340.373476
106.305338420.26406431.546897.766.305337.43.30.262088
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.373354440.16655961.842369.17.26.373353.860.173204
126.43636945.80.10468250.8597464.33.36.4363694.80.101028
136.4737746.80.062637740.4154172.11.66.47377.14.20.0554041
146.63540550.30.027638520.08544040.40.36.635404.71.60.0299414
156.73941651.70.12860220.5076922.526.739415.72.50.116569
166.79642252.40.039745560.3851931.91.56.7964245.90.0370206
176.87243153.50.28788121.27426.356.872431.22.50.291855
186.953443550.018035230.1158520.60.56.953443.13.50.0187895
197.04145656.60.010145140.06713010.30.37.041456.44.10.009237
207.18947859.40.43149743.4922817.313.67.189478.34.50.44081
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.36950562.70.1037460.8741714.33.47.369506.25.60.0884301
228.5571488.70.037954960.40534121.68.55714.26.50.0353211
238.63673491.20.16833751.382876.95.48.636733.64.70.16687
249.09877295.90.1532871.718218.56.79.098769.96.80.143149
259.317789980.033051630.1533220.80.69.317789.32.70.0323776
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55364.50.077007350.64416786.34.5537.15.40.0671744
25.212515.50.10468550.7901629.87.75.2125.14.60.0976609
35.8523028.60.14244940.87766210.98.55.852303.50.140385
46.5539649.20.11021971.2966216.112.66.55393.570.103817
56.8542853.20.041612960.39964453.96.85429.66.10.0366684
67.3550162.20.084366350.6525658.16.37.35500.44.60.0801985
78.15628780.0677946101.0671113.210.48.15628.39.60.0630399
88.4568885.50.10732371.3048216.212.78.45687.57.20.101656
98.6573691.40.054946960.6167467.768.65735.96.20.0563181
109.378797.80.04965750.4105955.149.3786.34.70.0487831
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 107
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 28 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln