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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1599 IGR1601 IGR1600 IGR1603 IGR1597 IGR1602 IGR1598 dtd, - SMu1855 SMu1857 ptaA,ptsG, - SMu1858 relA, - SMu1856 dexT, - SMu1854 SMu1859 treR, - SMu1853 dtd, - SMu1855 SMu1857 ptaA,ptsG, - SMu1858 relA, - SMu1856 dexT, - SMu1854 SMu1859 treR, - SMu1853 dtd, - SMu1855 SMu1857 ptaA,ptsG, - SMu1858 relA, - SMu1856 dexT, - SMu1854 SMu1859 treR, - SMu1853


LANL Gene ID: SMu1856

GenBank Locus Tag: SMU.2044c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
relA  

Definition:
GTP pyrophosphokinase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1919189

Gene Stop:
1916967

Gene Length:
2223

Molecular Weight*:
84088

pI*:
8.20

Net Charge*:
8.53

EC:
2.7.6.5  3.1.7.2  

Functional Class:
Regulatory functions; Other  

Gene Ontology:
Biological process
  GO:0008152    metabolic process
  GO:0015969    guanosine tetraphosphate metabolic process

Molecular function
  GO:0003824    catalytic activity
  GO:0016597    amino acid binding


Pathway: pathway table
Purine metabolism

Primary Evidence:
Mechold,U., Steiner,K., Vettermann,S. and Malke,H. Genetic organization of the streptokinase region of the Streptococcus equisimilis H46A chromosome Mol. Gen. Genet. 241 (1-2), 129-140 (1993) PubMed: 8232196.


Comment:
From GenBank (gi:2498843): In eubacteria, PPGPP (guanosine 3'-diphosphate 5-'diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyses the formation of PPPGPP which is then hydrolysed to form PPGPP. The reaction: ATP + GTP = AMP + guanosine 3'-diphosphate 5-'triphosphate, is the first step in the metabolism of PPGPP. This protein belongs to the RelA/SpoT family.



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Numerous strong matches in gapped BLAST to GTP pyrophosphokinase; residues 1-740 are 75% similar to GTP pyrophosphokinase of Streptococcus pneumoniae (gi15901481); residues 1-740 are 82% similar to stringent response-like protein of Streptococcus equisimilis (gi2498843).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1940 (0.0).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24380383|ref|NP_722338.1|  putative stringent response pr...  1486   0.0  
 gi|2498843|sp|Q54089|RELA_STREQ  Putative GTP pyrophosphokin...  1258   0.0  
 gi|94991264|ref|YP_599364.1|  GTP pyrophosphokinase / Guanos...  1254   0.0  
 gi|77411860|ref|ZP_00788193.1|  GTP pyrophosphokinase family...  1253   0.0  
 gi|19746884|ref|NP_608020.1|  (p)ppGpp synthetase [Streptoco...  1246   0.0  
 gi|71911499|ref|YP_283049.1|  GTP pyrophosphokinase/guanosin...  1241   0.0  
 gi|116627099|ref|YP_819718.1|  Guanosine polyphosphate pyrop...  1241   0.0  
 gi|94995176|ref|YP_603274.1|  GTP pyrophosphokinase / Guanos...  1240   0.0  
 gi|94989319|ref|YP_597420.1|  GTP pyrophosphokinase [Strepto...  1239   0.0  
 gi|28896614|ref|NP_802964.1|  putative (p)ppGpp synthetase [...  1239   0.0


InterPro Summary:  InterProScan

InterPro
IPR002912
Domain
Amino acid-binding ACT
PF01842 [664-731]T 0.140000007335441 PF01842 ACT ACT
InterPro
IPR003607
Domain
Metal-dependent phosphohydrolase, HD region
SM00471 [46-158]T 1.9E-8 SM00471 HDc HDc
InterPro
IPR004095
Domain
TGS
PF02824 [395-454]T 5.59999989416987E-26 PF02824 TGS TGS
InterPro
IPR004811
Family
RelA/SpoT protein
TIGR00691 [31-737]T 0.0 TIGR00691 spoT_relA spoT_relA
InterPro
IPR006674
Domain
Metal-dependent phosphohydrolase, HD region, subdomain
PF01966 [50-149]T 4.19999771339582E-7 PF01966 HD HD
InterPro
IPR007685
Domain
RelA/SpoT
PF04607 [240-349]T 2.39999798157241E-48 PF04607 RelA_SpoT RelA_SpoT
InterPro
IPR012675
Domain
Beta-grasp fold, ferredoxin-type
G3DSA:3.10.20.30 [394-457]T 2.5999994671899602E-6 G3DSA:3.10.20.30 Ferredoxin_fold Ferredoxin_fold
InterPro
IPR012676
Domain
TGS-like
SSF81271 [395-455]T 4.0E-20 SSF81271 TGS-like TGS-like
noIPR
unintegrated
unintegrated
PTHR21262 [121-740]T 0.0 PTHR21262 PTHR21262 PTHR21262
SSF109604 [5-196]T 1.2999999999999998E-78 SSF109604 SSF109604 SSF109604
SSF55021 [658-738]T 2.3E-10 SSF55021 SSF55021 SSF55021
SSF81301 [197-371]T 3.7999999999999996E-68 SSF81301 SSF81301 SSF81301


COGS Summary:  COGS Search
BeTs to 9 clades of COG0317
COG name: Guanosine polyphosphate pyrophosphohydrolases/synthetases
Functional Class: T,K
The phylogenetic pattern of COG0317 is -----qvcEbrHujgpo----
Number of proteins in this genome belonging to this COG is 3
Blocks Summary:  Blocks Search
No significant hits to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 195-349 are 89% similar to a (SYNTHETASE PROTEOME COMPLETE KINASE) protein domain (PD002902 which is seen in RELA_STREQ.

Residues 35-148 are 64% similar to a (SYNTHETASE COMPLETE PROTEOME) protein domain (PD002764 which is seen in RELA_STAAU.

Residues 322-380 are 45% similar to a (MANGANESE 3'-PYROPHOSPHOHYDROLASE) protein domain (PD213177 which is seen in SPOT_AQUAE.

Residues 313-388 are 33% similar to a (PROTEOME 3'-PYROPHOSPHOHYDROLASE COMPLETE) protein domain (PD272244 which is seen in Q9PN26_CAMJE.

Residues 149-192 are 72% similar to a (3'-PYROPHOSPHOHYDROLASE PROTEOME SYNTHETASE COMPLETE) protein domain (PD371068 which is seen in RELA_STRCO.

Residues 108-148 are 58% similar to a (PROTEOME PPPGPP TRANSFERASE COMPLETE) protein domain (PD405475 which is seen in Q9RH69_BRAJA.

Residues 149-194 are 73% similar to a (SYNTHETASE GTP KINASE PYROPHOSPHOKINASE) protein domain (PD334556 which is seen in RELA_STAAU.

Residues 601-737 are 25% similar to a (HYDROLASE KINASE/PHOSPHOHYDROLASE) protein domain (PD118638 which is seen in O69970_STRCO.

Residues 350-392 are 81% similar to a (SYNTHETASE ATP:GTP PPPGPP RESPONSE-LIKE) protein domain (PD375265 which is seen in RELA_STREQ.

Residues 613-697 are 30% similar to a (PPPGPP TRANSFERASE SYNTHETASE HOMOLOG) protein domain (PD414392 which is seen in Q9RH69_BRAJA.

Residues 393-737 are 78% similar to a (SYNTHETASE COMPLETE PROTEOME KINASE) protein domain (PD003989 which is seen in RELA_STREQ.

Residues 184-218 are 77% similar to a (PYROPHOSPHOKINASE PROTEOME COMPLETE GTP SYNTHETASE PPGPP) protein domain (PD253748 which is seen in Q9CJ94_LACLA.

Residues 149-183 are 97% similar to a (HYDROLASE COMPLETE) protein domain (PD368508 which is seen in RELA_STREQ.

Residues 108-148 are identical to a (SYNTHETASE PPGPP PROTEOME GTP) protein domain (PD378346 which is seen in Q9CJ94_LACLA.


Paralogs:  Local Blast Search
SMu1856 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search
Residues 50 to 149 (E-value = 2.2e-10) place SMu1856 in the HD family which is described as HD domain (PF01966)
Residues 240 to 351 (E-value = 2.1e-75) place SMu1856 in the RelA_SpoT family which is described as Region found in RelA / SpoT proteins (PF04607)
Residues 391 to 454 (E-value = 8.5e-30) place SMu1856 in the TGS family which is described as TGS domain (PF02824)
Residues 664 to 738 (E-value = 4.4e-06) place SMu1856 in the ACT family which is described as ACT domain (PF01842)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
707  
723

Top PDB Hits:
No significant hits to the NCBI PDB database.

Gene Protein Sequence:
MAKDANLTGQEVIDICSMYMNKADIAFVQKALDYATECHSGQFRKSGEPY
IIHPIQVAGILADLHLDAITVSCGFLHDVIEDTEATLDHLEAKFGSDVKN
IVDGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMR
TLRHLRKDKQERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNEVEF
YKISHLMNEKRREREALVDDIVDKIKAYTAEQGLHGEIYGRPKHIYSIYR
KMRDKKKRFDQIYDLIAIRCILDTQSDVYAMLGYIHELWRPMPGRFKDYI
ANPKANGYQSIHTTVYGPKGPIEVQIRTHEMHQVAEYGVAAHWAYKKGIK
GKVNSQESALGMNWIKELVELQDASNGNAVDFVDSVKEDIFAERIYVFTP
NGAVQELPKDSGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVV
EIITNSNSFGPSRDWINIVKTNKARNKIRQFFKNQDKELSITKGRDLLVD
YFQEQGYVANKYLDRKHIEAVLNRLNYRNEGALYAAVGFGELSPVTVFNK
LTEKERREQERAKAKAEAEELMKGGEVKHDNKKEVLKVKSEGGVIVQGSS
GLLMRIAKCCNPVPGDPIEGYITKGRGVAIHRADCHNIKSQEGYEERLID
VEWEDDYSTKEYMAEIDIYGLNRAGLLNDVLQILSNQTKNISTVNAQPTK
DMKFANIHVTFGIPNLTVLTAVVDKIKVIPDVYSVKRTNG

Gene Nucleotide Sequence:  Sequence Viewer
ATGGCGAAAGATGCAAATTTAACAGGTCAAGAGGTTATTGATATCTGCTC
AATGTATATGAATAAAGCAGATATTGCCTTTGTTCAAAAAGCTTTAGATT
ATGCCACGGAATGCCATAGCGGTCAATTTCGTAAGTCAGGGGAACCTTAT
ATTATTCATCCTATTCAGGTTGCAGGAATTTTAGCCGACTTACATTTGGA
TGCTATTACAGTTTCCTGTGGCTTTTTGCATGATGTCATTGAAGATACCG
AAGCAACTTTAGATCATTTAGAAGCTAAATTTGGCAGTGATGTTAAGAAC
ATTGTTGATGGTGTCACAAAGCTAGGGAAAGTTGAGTATAAATCGCATGA
AGAACAGCTAGCAGAAAATCACCGTAAGATGTTGATGGCTATGTCTAAAG
ATATTCGTGTCATTCTGGTTAAATTAGCTGACCGTCTTCATAATATGCGG
ACGCTGAGGCATTTACGCAAGGATAAGCAAGAACGTATTTCACGCGAAAC
CATGGAAATTTATGCTCCCTTGGCGCATCGGCTGGGGATTAGTCGCATCA
AATGGGAATTAGAAGATTTAGCTTTTCGTTATCTTAATGAAGTTGAATTT
TATAAAATTTCGCATTTAATGAACGAAAAGCGTCGTGAACGTGAAGCATT
AGTTGATGATATTGTTGATAAAATTAAAGCTTATACTGCAGAGCAGGGCT
TACATGGTGAGATTTACGGACGTCCCAAACACATTTATTCCATTTATCGG
AAGATGCGTGATAAGAAGAAACGTTTTGATCAAATTTATGATTTGATCGC
TATTCGTTGTATTTTGGATACACAAAGTGATGTTTATGCCATGCTGGGTT
ATATTCATGAGCTTTGGCGTCCTATGCCCGGTCGTTTTAAGGACTATATT
GCTAACCCTAAAGCAAATGGTTACCAATCCATTCATACAACTGTTTATGG
ACCCAAAGGGCCAATTGAAGTACAAATTCGGACGCATGAAATGCATCAAG
TGGCTGAATATGGGGTAGCAGCTCACTGGGCTTATAAAAAGGGAATCAAA
GGTAAGGTTAACAGTCAAGAATCAGCTCTAGGAATGAACTGGATTAAGGA
ACTTGTTGAGTTGCAAGACGCTTCCAATGGCAATGCGGTTGACTTTGTTG
ATTCTGTCAAAGAAGATATCTTTGCAGAGCGTATTTATGTTTTCACACCT
AATGGTGCTGTTCAAGAATTGCCTAAGGATTCTGGTCCTATTGACTTTGC
CTATGCCATTCATACACAGGTTGGAGAAAAAGCAACAGGCGCTAAAGTTA
ATGGTCGCATGGTCCCTTTAACAGCTAAGCTCAAAACAGGTGATGTGGTT
GAAATCATCACAAATTCCAATTCTTTTGGTCCCAGCCGTGACTGGATTAA
TATTGTTAAGACCAATAAAGCTCGTAATAAAATTCGTCAATTTTTTAAAA
ACCAAGATAAGGAACTCTCTATCACAAAAGGCCGTGATTTGCTGGTTGAT
TATTTTCAAGAGCAGGGCTATGTGGCCAATAAATATTTGGATAGAAAACA
TATAGAGGCTGTTTTGAATCGTCTCAACTACCGTAATGAAGGAGCTCTTT
ATGCGGCTGTCGGTTTTGGTGAATTGTCGCCTGTTACCGTCTTTAATAAA
TTGACAGAAAAAGAACGCCGTGAACAAGAACGTGCCAAAGCTAAAGCGGA
GGCTGAGGAACTCATGAAGGGCGGTGAAGTCAAGCACGACAATAAAAAAG
AAGTCCTTAAGGTTAAAAGTGAAGGCGGTGTTATTGTTCAAGGCTCCTCT
GGTCTTTTGATGCGGATTGCTAAGTGCTGTAACCCTGTCCCAGGCGATCC
TATTGAAGGCTATATCACAAAAGGGCGTGGCGTCGCTATTCATCGTGCTG
ACTGTCATAATATTAAGAGTCAAGAAGGCTATGAAGAGCGTTTGATTGAT
GTCGAATGGGAAGATGATTATTCCACCAAGGAATATATGGCAGAAATTGA
TATTTATGGTCTCAACCGTGCCGGTCTCTTAAATGATGTTCTGCAAATCT
TATCGAATCAAACAAAGAACATCTCAACGGTCAACGCCCAGCCAACTAAG
GATATGAAATTTGCCAATATCCATGTGACTTTTGGCATCCCTAACTTAAC
CGTTTTAACAGCTGTTGTTGATAAAATTAAGGTCATTCCAGATGTTTATT
CTGTTAAAAGGACTAACGGGTAA

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