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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1261 IGR1258 IGR1255 IGR1260 IGR1256 IGR1257 IGR1259 celB,licB, - SMu1455 SMu1459 celA,bglA, - SMu1456 celR,bglG,licR, - SMu1454 rnr,vacB1, - SMu1462 gloA,lguL, - SMu1458 frp, - SMu1457 SMu1460 celB,licB, - SMu1455 SMu1459 celA,bglA, - SMu1456 celR,bglG,licR, - SMu1454 rnr,vacB1, - SMu1462 gloA,lguL, - SMu1458 frp, - SMu1457 SMu1460 celB,licB, - SMu1455 SMu1459 celA,bglA, - SMu1456 celR,bglG,licR, - SMu1454 rnr,vacB1, - SMu1462 smpB, - SMu1461 smpB, - SMu1461 gloA,lguL, - SMu1458 frp, - SMu1457 SMu1460


LANL Gene ID: SMu1458

GenBank Locus Tag: SMU.1603

DNA Molecule Name:
1  

GenBank ID:
24379984

Gene Name:
gloA  lguL  

Definition:
lactoylglutathione lyase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1533897

Gene Stop:
1534289

Gene Length:
393

Molecular Weight*:
15255

pI*:
4.80

Net Charge*:
-7.85

EC:
4.4.1.5  

Functional Class:
Energy metabolism; Other  

Gene Ontology:
Biological process
  GO:0005975    carbohydrate metabolic process

Molecular function
  GO:0004462    lactoylglutathione lyase activity


Pathway: pathway table
Pyruvate metabolism

Secondary Evidence:
Kizil,G., Wilks,K., Wells,D. and Ala'Aldeen,D.A.
Detection and characterisation of the genes encoding glyoxalase I
and II from Neisseria meningitidis
J. Med. Microbiol. 49 (7), 669-673 (2000)
PubMed: 10882093


Comment:
From Genbank:[gi:1175222]
This enzyme catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Limited matches in gapped BLAST to lactoylglutathione lyase: residues 2-125 are 61% similar to the enzyme in S.pneumoniae (gi15902908). Residues 1-124 are 64% similar to the protein from S.pyogenes (gi15674615).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1478 (5e-63).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379984|ref|NP_721939.1|  putative lactoylglutathione ly...   241   9e-63
 gi|55821508|ref|YP_139950.1|  glyoxalase I/lactoylglutathion...   226   2e-58
 gi|116628224|ref|YP_820843.1|  Lactoylglutathione lyase or r...   225   6e-58
 gi|77408563|ref|ZP_00785299.1|  lactoylglutathione lyase [St...   213   2e-54
 gi|22537621|ref|NP_688472.1|  lactoylglutathione lyase [Stre...   213   3e-54
 gi|29375716|ref|NP_814870.1|  lactoylglutathione lyase [Ente...   176   5e-43
 gi|69245105|ref|ZP_00603229.1|  Glyoxalase/bleomycin resista...   165   9e-40
 gi|47095742|ref|ZP_00233348.1|  lactoylglutathione lyase, pu...   154   1e-36
 gi|116873600|ref|YP_850381.1|  lactoylglutathione lyase, put...   154   2e-36
 gi|46908401|ref|YP_014790.1|  lactoylglutathione lyase, puta...   154   2e-36


InterPro Summary:  InterProScan

InterPro
IPR004360
Domain
Glyoxalase/bleomycin resistance protein/dioxygenase
PF00903 [2-121]T 2.10000267834025E-23 PF00903 Glyoxalase Glyoxalase
InterPro
IPR004361
Family
Glyoxalase I
TIGR00068 [3-129]T 6.25630178763941E-36 TIGR00068 glyox_I glyox_I
PS00935 [68-80]T 8.0E-5 PS00935 GLYOXALASE_I_2 GLYOXALASE_I_2
InterPro
IPR011588
Domain
Glyoxalase/extradiol ring-cleavage dioxygenase
PD002334 [4-121]T 0.0 PD002334 Gly_diox Gly_diox
noIPR
unintegrated
unintegrated
G3DSA:3.10.180.10 [2-122]T 6.100026561498099E-45 G3DSA:3.10.180.10 G3DSA:3.10.180.10 G3DSA:3.10.180.10
PTHR10374 [1-122]T 1.7000029559006503E-22 PTHR10374 PTHR10374 PTHR10374
SSF54593 [1-121]T 2.4000000000000002E-30 SSF54593 SSF54593 SSF54593


COGS Summary:  COGS Search
BeTs to 7 clades of COG0346
COG name: Lactoylglutathione lyase
Functional Class: E
The phylogenetic pattern of COG0346 is A-tky--CEBRh---------
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** IPB000325 (Glyoxalase I) with a combined E-value of 9.2e-36.
    IPB000325A    5-43
    IPB000325B    54-63
    IPB000325C    69-103
    IPB000325D    108-120
    IPB000325C    1-35


 ProDom Summary:  Protein Domain Search
Residues 9-122 are 48% similar to a (LYASE I GLYOXALASE LACTOYLGLUTATHIONE) protein domain (PD002334 which is seen in LGUL_HAEIN.

Residues 5-120 are 32% similar to a (RESISTANCE PROTEOME FOSFOMYCIN COMPLETE) protein domain (PD016133 which is seen in Q9KBZ6_BACHD.


Paralogs:  Local Blast Search
SMu1458 is paralogously related (blast p-value < 1e-3) to SMu1015, a predicted conserved hypothetical protein.


Pfam Summary:  Pfam Search
Residues 2 to 121 (E-value = 1.2e-27) place SMu1458 in the Glyoxalase family which is described as Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903)

 Top PDB Hits:
pdb1F9ZA Chain A, Crystal Structure Of The Ni(Ii)-Bound Glyox... 97 9e-022
pdb1QIPB Chain B, Human Glyoxalase I Complexed With S-P- Nitr... 66 2e-012
pdb1BH5B Chain B, Human Glyoxalase I Q33e, E172q Double Mutan... 65 4e-012

Gene Protein Sequence:
MKFLHTCIRVKDLDASLKFYQEALDFKEVRRNDFPEYKFTLVYLQLEDDP
DYELELTYNYDHEAYDLGNGYGHIAVGVDDLEATHQAHQKAGYTVTDLSG
LPGKPKMYYFITDPDGYKIEVIRLKQFQEK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAATTTTTACATACCTGTATCCGTGTTAAGGACTTAGACGCTTCGCT
TAAATTTTATCAAGAGGCACTAGACTTCAAAGAAGTTCGCCGCAATGATT
TTCCTGAATACAAATTTACTCTGGTTTATCTGCAACTAGAAGACGACCCC
GACTATGAGTTAGAATTAACTTACAATTACGATCACGAAGCTTATGACCT
TGGCAATGGCTATGGTCATATCGCTGTCGGTGTTGATGACCTTGAAGCGA
CTCATCAAGCGCATCAAAAAGCCGGCTATACTGTGACAGACCTTTCAGGT
CTGCCCGGTAAGCCAAAGATGTACTACTTCATCACAGACCCCGATGGCTA
TAAGATCGAAGTTATCCGTCTCAAACAATTTCAGGAAAAATAA

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