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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1252 IGR1253 IGR1255 IGR1248 IGR1249 IGR1246 IGR1247 IGR1256 IGR1251 celC,licA, - SMu1453 celB,licB, - SMu1455 SMu1452 SMu1448 cah, - SMu1450 regM,ccpA, - SMu1446 celB,lacE, - SMu1451 celA,bglA, - SMu1456 amy, - SMu1445 celR,bglG,licR, - SMu1454 pepQ, - SMu1447 celC,licA, - SMu1453 celB,licB, - SMu1455 SMu1452 SMu1448 cah, - SMu1450 regM,ccpA, - SMu1446 celB,lacE, - SMu1451 celA,bglA, - SMu1456 amy, - SMu1445 celR,bglG,licR, - SMu1454 pepQ, - SMu1447 celB,licB, - SMu1455 SMu1452 SMu1448 cah, - SMu1450 regM,ccpA, - SMu1446 celB,lacE, - SMu1451 celA,bglA, - SMu1456 amy, - SMu1445 celR,bglG,licR, - SMu1454 celC,licA, - SMu1453 pepQ, - SMu1447


LANL Gene ID: SMu1451

GenBank Locus Tag: SMU.1596c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
celB  lacE  

Definition:
PTS system, cellobiose-specific IIC component

Cellular Location:
Membrane, Cytoplasm [Evidence]

Gene Start:
1527980

Gene Stop:
1526622

Gene Length:
1359

Molecular Weight*:
49235

pI*:
8.30

Net Charge*:
2.34

EC:
2.7.1.69  

Functional Class:
Signal transduction; PTS  

Gene Ontology:
Biological process
  GO:0009401    phosphoenolpyruvate-dependent sugar phosphotransferase system

Cellular component
  GO:0016020    membrane
  GO:0016021    integral to membrane

Molecular function
  GO:0005351    sugar porter activity
  GO:0008982    protein-N(PI)-phosphohistidine-sugar phosphotransferase activity


Pathway: pathway table
Aminosugars metabolism
Fructose and mannose metabolism
Galactose metabolism
Glycolysis / Gluconeogenesis
Starch and sucrose metabolism

Primary Evidence:
Old LA, Lowes S, Russell RR.
Genomic variation in Streptococcus mutans: deletions affecting the multiple pathways of beta-glucoside metabolism.
Oral Microbiol Immunol. 2006 Feb;21(1):21-7.
PMID: 16390337

Rosey EL, Stewart GC.
Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans.
J Bacteriol. 1992 Oct;174(19):6159-70.
PMID: 1400164

Honeyman AL, Curtiss R.
Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene.
J Gen Microbiol. 1993 Nov;139(11):2685-94.
PMID: 8277252

Secondary Evidence:
Lai,X. and Ingram,L.O.
Cloning and sequencing of a cellobiose phosphotransferase system
operon from Bacillus stearothermophilus XL-65-6 and functional
expression in Escherichia coli
J. Bacteriol. 175 (20), 6441-6450 (1993)
PubMed: 8407820


Comment:
For other 'lac' genes see SMu0104 (lacD); SMu0793 (lacR); SMu0794 (lacC); SMu0094 (lacI); SMu1359 (lacC);SMu1354 (lacX); SMu1355 (lacG); SMu1356 (lacE);SMu1358 (lacD);SMu1360 (lacB); SMu1361 (lacA) and SMu1362 (lacR).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to PTS system, sugar-specific IIC component:residues 28-450 are 28% similar to the previously published protein in S.mutans (433809),(1709898).

Residues 1-446 are 72% similar to the enzyme from S.pneumoniae
(15900243). This sequence is similar to Spy2050.





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0330 (3e-49).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379977|ref|NP_721932.1|  putative PTS system, cellobios...   803   0.0  
 gi|157076404|gb|ABV11087.1|  PTS system, IIC component [Stre...   660   0.0  
 gi|81096840|ref|ZP_00875168.1|  PTS lactose/cellobiose IIC c...   639   0.0  
 gi|148983712|ref|ZP_01817031.1|  PTS system, IIC component [...   637   0.0  
 gi|148988068|ref|ZP_01819531.1|  PTS system, IIC component [...   637   0.0  
 gi|149006059|ref|ZP_01829788.1|  PTS system, IIC component [...   636   0.0  
 gi|149018002|ref|ZP_01834461.1|  PTS system, IIC component [...   635   e-180
 gi|15900243|ref|NP_344847.1|  PTS system, IIC component [Str...   634   e-180
 gi|15902326|ref|NP_357876.1|  Phosphotransferase system suga...   630   e-179
 gi|69244103|ref|ZP_00602639.1|  PTS lactose/cellobiose IIC c...   492   e-137


InterPro Summary:  InterProScan

InterPro
IPR003352
Domain
Phosphotransferase system, EIIC
PF02378 [32-373]T 7.000038084225991E-75 PF02378 PTS_EIIC PTS_EIIC
InterPro
IPR004501
Domain
Phosphotransferase system, lactose/cellobiose IIC component
TIGR00410 [15-447]T 3.20112911140192E-74 TIGR00410 lacE lacE
PS51105 [11-433]T 0.0 PS51105 PTS_EIIC_TYPE_3 PTS_EIIC_TYPE_3


COGS Summary:  COGS Search
BeTs to 3 clades of COG1455
COG name: Phosphotransferase system cellobiose-specific component IIC
Functional Class: G
The phylogenetic pattern of COG1455 is --------eB------o----
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
No significant hits to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 175-447 are 38% similar to a (COMPONENT SYSTEM PHOSPHOTRANSFERASE PTS) protein domain (PD006461 which is seen in O50980_BORBU.


Paralogs:  Local Blast Search
SMu1451 is paralogously related (blast p-value < 1e-3) to SMu1356,a predicted PTS system, lactose-specific component IIBC; SMu0159,a predicted hypothetical protein (possible PTS system component) and SMu1677, a predicted PTS system, sucrose-specific IIABC component.


Pfam Summary:  Pfam Search
Residues 32 to 373 (E-value = 4.4e-77) place SMu1451 in the PTS_EIIC family which is described as Phosphotransferase system, EIIC (PF02378)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
38  
54
transmembrane  
120  
136
transmembrane  
168  
184
transmembrane  
216  
232
transmembrane  
256  
272
transmembrane  
309  
325
transmembrane  
354  
370
transmembrane  
415  
431

Top PDB Hits:
No significant hits to the NCBI PDB database.

Gene Protein Sequence:
MDEQKGLFGFLDKYLMGPMSIIAQYKVVRAITAAGMAVVPFTIVGSMVLV
FSVLPISFSFLPIVKDIFAASFDKFTALYMIINYATMGSLSLYFVLTMGY
ELTKIYAEEEELNMNPLNGALLSLFAFIMTVPQIVFNNGSMSSVTTLKKG
AVTVDGWAMSNGVTRFGTTGIFTAIIMGILTVLLYRLCIKRNWVIKMPEA
VPEGVSRGFSALIPGFVIAFVVIFIDGIFIFLGTDIFEAISIPFSFVRNL
TGTWIGIMIIYLLTQALWIVGIHGANIIFAFVNPIALANMAENAKGAHYI
VAGEWSNMFVIAGGSGATLGLCIWLATRSKSEQLNAIGKASVVPAIFNIN
EPLIFGIPIIYNPALAIPFILAPMTSATLYYLAMKLDLIKAVFANVPWPT
PVGIGAFIGTGDWKSVILALICAVAAFLVYYPFIRNYDRKLVKEEREASA
IA

Gene Nucleotide Sequence:  Sequence Viewer
ATGGATGAACAAAAAGGATTATTTGGTTTCCTAGATAAATATTTGATGGG
ACCGATGAGCATTATTGCTCAATATAAAGTTGTACGCGCTATTACAGCAG
CGGGTATGGCTGTTGTTCCTTTTACCATTGTTGGTTCAATGGTTTTGGTT
TTTAGTGTTTTACCGATATCGTTTTCTTTCTTGCCTATTGTCAAAGATAT
TTTTGCAGCTTCCTTTGATAAGTTTACTGCCTTATATATGATTATTAATT
ATGCAACTATGGGTTCCTTGTCGCTTTACTTTGTTCTAACTATGGGTTAT
GAGTTAACCAAAATTTATGCAGAAGAAGAGGAACTAAATATGAATCCTCT
AAACGGGGCTCTGCTGTCATTATTCGCTTTTATCATGACTGTTCCACAAA
TTGTTTTTAATAATGGTTCCATGTCATCTGTGACAACTCTTAAAAAAGGA
GCAGTTACTGTAGATGGCTGGGCAATGAGTAATGGTGTTACTCGATTTGG
CACAACAGGGATTTTTACTGCTATTATCATGGGAATATTAACAGTTCTTC
TTTATCGTCTATGTATAAAACGCAATTGGGTAATAAAAATGCCAGAAGCG
GTACCAGAAGGGGTCTCTCGAGGTTTTTCAGCTTTAATCCCTGGTTTTGT
CATTGCATTTGTTGTTATATTTATTGATGGTATCTTTATTTTTCTAGGAA
CTGATATTTTTGAAGCCATATCTATTCCATTTAGTTTTGTTAGAAACTTA
ACTGGAACATGGATTGGTATTATGATTATTTATCTTCTAACCCAAGCACT
TTGGATTGTTGGGATTCATGGCGCTAATATTATCTTTGCTTTTGTTAATC
CAATTGCTCTTGCTAATATGGCAGAAAATGCTAAGGGAGCTCATTATATT
GTTGCAGGTGAATGGTCCAATATGTTTGTTATCGCTGGTGGATCTGGTGC
AACTCTTGGACTTTGTATTTGGTTAGCAACTCGTTCTAAGTCTGAACAAT
TGAATGCTATTGGTAAAGCATCTGTTGTACCAGCTATTTTCAATATTAAT
GAACCTTTAATTTTTGGTATTCCAATTATCTATAACCCAGCACTTGCTAT
TCCATTTATTTTAGCACCAATGACTTCTGCAACCCTTTATTATTTAGCAA
TGAAATTAGATTTGATTAAGGCAGTTTTTGCCAATGTTCCATGGCCAACA
CCGGTTGGTATTGGTGCTTTTATTGGTACAGGAGATTGGAAATCTGTCAT
TTTAGCTCTAATTTGTGCTGTAGCTGCTTTTCTTGTCTATTATCCATTCA
TTAGAAATTATGATAGAAAACTAGTCAAAGAAGAACGGGAAGCTTCAGCA
ATTGCTTAA

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