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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1252 IGR1248 IGR1245 IGR1249 IGR1246 IGR1247 IGR1243 IGR1251 SMu1452 SMu1448 SMu1442 cah, - SMu1450 ywaF,cpoA, - SMu1444 regM,ccpA, - SMu1446 rfaG, - SMu1443 celB,lacE, - SMu1451 amy, - SMu1445 pepQ, - SMu1447 SMu1452 SMu1448 SMu1442 cah, - SMu1450 ywaF,cpoA, - SMu1444 regM,ccpA, - SMu1446 rfaG, - SMu1443 celB,lacE, - SMu1451 amy, - SMu1445 pepQ, - SMu1447 SMu1448 SMu1442 cah, - SMu1450 ywaF,cpoA, - SMu1444 regM,ccpA, - SMu1446 rfaG, - SMu1443 celB,lacE, - SMu1451 amy, - SMu1445 SMu1452 pepQ, - SMu1447


LANL Gene ID: SMu1446

GenBank Locus Tag: SMU.1591c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
regM  ccpA  

Definition:
catabolite control protein A

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1523545

Gene Stop:
1522544

Gene Length:
1002

Molecular Weight*:
36601

pI*:
6.90

Net Charge*:
-0.29

EC:
 

Functional Class:
Regulatory functions; DNA interactions  

Gene Ontology:
Biological process
  GO:0006355    regulation of transcription, DNA-dependent
  GO:0045449    regulation of transcription

Cellular component
  GO:0005622    intracellular

Molecular function
  GO:0003700    transcription factor activity
  GO:0030528    transcription regulator activity


Pathway: pathway table

Primary Evidence:
Simpson,C.L. and Russell,R.R.
Identification of a homolog of CcpA catabolite repressor protein in Streptococcus mutans
Infect. Immun. 66 (5), 2085-2092 (1998)
PubMed: 9573093

Browngardt CM, Wen ZT, Burne RA.
RegM is required for optimal fructosyltransferase and glucosyltransferase gene expression in Streptococcus mutans.
FEMS Microbiol Lett. 2004 Nov;240(1):75-9.
PMID: 15500982

Secondary Evidence:
van den Bogaard,P.T.C., Kleerebezem,M., Kuipers,O.P. and de
Vos,W.M.
Control of Lactose Transport, beta-Galactosidase Activity, and
Glycolysis by CcpA in Streptococcus thermophilus: Evidence for
Carbon Catabolite Repression by a Non- Phosphoenolpyruvate- Dependent Phosphotransferase System Sugar
J. Bacteriol. 182 (21), 5982-5989 (2000)
PUBMED: 11029416


Comment:


View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to catabolite control protein A: residues 1-333 are 95% similar to a previously published enzyme in S.mutans (gi2323342), (gi2495405) and (gi2155300).

Residues 1-333 are 84% similar to the protein from S.bovis
(gi7328454).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0707 (1e-160).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379973|ref|NP_721928.1|  catabolite control protein A, ...   627   e-178
 gi|2323342|gb|AAC46294.1|  RegM [Streptococcus mutans]            626   e-178
 gi|2155300|gb|AAB58798.1|  catabolite control protein A [Str...   623   e-177
 gi|22536871|ref|NP_687722.1|  transcriptional regulator, Reg...   563   e-159
 gi|76788388|ref|YP_329455.1|  catabolite control protein A [...   561   e-158
 gi|94989934|ref|YP_598034.1|  Catabolite control protein A [...   541   e-152
 gi|19745632|ref|NP_606768.1|  catabolite control protein A [...   540   e-152
 gi|118582218|gb|ABL07487.1|  catabolite control protein A [S...   540   e-152
 gi|7328454|dbj|BAA88121.3|  catabolite control protein A [St...   539   e-152
 gi|15674618|ref|NP_268792.1|  catabolite control protein A [...   539   e-151


InterPro Summary:  InterProScan

InterPro
IPR000843
Domain
Bacterial regulatory protein, LacI
PR00036 [8-18]T 1.7E-8 PR00036 HTHLACI[18-28]T 1.7E-8 PR00036 HTHLACI HTHLACI
PF00356 [7-32]T 4.30000170645879E-11 PF00356 LacI LacI
SM00354 [6-76]T 9.89999999999886E-38 SM00354 HTH_LACI HTH_LACI
PS00356 [9-27]T 8.0E-5 PS00356 HTH_LACI_1 HTH_LACI_1
PS50932 [7-61]T 0.0 PS50932 HTH_LACI_2 HTH_LACI_2
InterPro
IPR001761
Domain
Periplasmic binding protein/LacI transcriptional regulator
PF00532 [64-328]T 9.499999851064279E-4 PF00532 Peripla_BP_1 Peripla_BP_1
InterPro
IPR006377
Family
Catabolite control protein A
TIGR01481 [5-333]T 0.0 TIGR01481 ccpA ccpA
InterPro
IPR010982
Domain
Lambda repressor-like, DNA-binding
SSF47413 [5-63]T 1.2E-20 SSF47413 Lambda_like_DNA Lambda_like_DNA
noIPR
unintegrated
unintegrated
G3DSA:3.40.50.2300 [58-196]T 1.2000011745813999E-30 G3DSA:3.40.50.2300 G3DSA:3.40.50.2300 G3DSA:3.40.50.2300
SSF53822 [65-333]T 2.6E-70 SSF53822 SSF53822 SSF53822


COGS Summary:  COGS Search
BeTs to 4 clades of COG1609
COG name: Transcriptional regulators of the LacI family
Functional Class: K
The phylogenetic pattern of COG1609 is ------V-EBrH---------
Number of proteins in this genome belonging to this COG is 5
Blocks Summary:  Blocks Search
***** IPB000843 (Bacterial regulatory protein, LacI family) with a combined E-value of 8.1e-37.
    IPB000843A    8-28
    IPB000843B    33-71


 ProDom Summary:  Protein Domain Search
Residues 8-90 are 83% similar to a (TRANSCRIPTION DNA-BINDING REGULATION REPRESSOR) protein domain (PD000947 which is seen in Q48518_LACCA.

Residues 91-142 are 75% similar to a (TRANSCRIPTION REGULATION DNA-BINDING) protein domain (PD329029 which is seen in Q48518_LACCA.

Residues 68-125 are identical to a (TRANSCRIPTION REGULATION DNA-BINDING REPRESSOR) protein domain (PD353269 which is seen in CCPA_STRMU.

Residues 280-333 are 96% similar to a (TRANSCRIPTION DNA-BINDING REGULATION TRANSCRIPTIONAL) protein domain (PD009758 which is seen in CCPA_STRMU.

Residues 8-125 are 43% similar to a (TRANSCRIPTION REPRESSOR REGULATION) protein domain (PD023303 which is seen in Q9WWF6_AGRTU.

Residues 143-277 are 89% similar to a (TRANSCRIPTION DNA-BINDING REGULATION REPRESSOR) protein domain (PD000591 which is seen in CCPA_STRMU.

Residues 9-64 are 51% similar to a (DNA-BINDING TRANSCRIPTION REGULATION) protein domain (PD407822 which is seen in O87640_STRCO.


Paralogs:  Local Blast Search
SMu1446 is paralogously related (blast p-value < 1e-3) to SMu1424, SMu1679 ,SMu0094 and SMu0807,all lacI family and related sugar operon repressors.

Pfam Summary:  Pfam Search
Residues 7 to 32 (E-value = 4.8e-13) place SMu1446 in the LacI family which is described as Bacterial regulatory proteins, lacI family (PF00356)
Residues 64 to 330 (E-value = 3.8e-09) place SMu1446 in the Peripla_BP_1 family which is described as Periplasmic binding proteins and sugar binding domain of the LacI family (PF00532)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
67  
83

Top PDB Hits:
pdb1BDHA Chain A, Purine Repressor Mutant-Hypoxanthine-Palind... 123 4e-029
pdb2PUEA Chain A, Crystal Structure Of The Laci Family Member... 122 8e-029
pdb2PUAA Chain A, Crystal Structure Of The Laci Family Member... 121 1e-028

Gene Protein Sequence:
MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEVIDRLDY
RPNAVARGLASKKTTTVGVVIPNIANAYFSILAKGIDDIATMYKYNIVLA
SSDEDDDKEVNVINTLFAKQVDGIIFMGHHLTEKIRAEFSRARTPVVLSG
TVDLEHQLPSVNIDHSKAAQDAVALLAKHHDKIAFVSGPLIDDINGKVRL
AGYKEGLKKKGLPFKEGLVFEAQYKYQEGYQLAQRVINSGATAAYVAEDE
LAAGLLNGLFAAGKKVPEDFEIITSNDSTIALYTRPNMTSISQPIYDLGA
VAMRMLTKIMNKEELEEKEIVLNHGIRERGTTK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAACACAGATGACACAATCACTATTTATGATGTTGCCCGTGAAGCGGG
AGTGTCCATGGCAACTGTCAGCCGTGTTGTGAATGGAAATAAAAATGTCA
AAGAGAATACCCGCAAAAAAGTCCTAGAAGTCATTGACCGTCTTGATTAT
CGGCCAAATGCTGTGGCGCGTGGTTTGGCAAGTAAGAAAACGACTACGGT
AGGTGTGGTTATCCCTAATATTGCTAATGCTTATTTTTCTATTTTAGCTA
AGGGAATTGATGACATTGCAACCATGTATAAATATAATATTGTACTGGCT
TCAAGTGATGAAGATGACGATAAGGAAGTCAATGTTATCAATACTTTATT
TGCTAAACAGGTTGATGGTATTATTTTTATGGGACATCATTTAACTGAAA
AAATTCGCGCTGAATTTTCACGAGCACGGACACCGGTTGTTTTATCAGGT
ACTGTAGATCTGGAGCATCAACTCCCCAGTGTTAATATTGATCATAGTAA
GGCAGCACAAGATGCCGTAGCTTTATTGGCCAAACATCATGATAAGATTG
CTTTTGTATCGGGTCCCCTTATTGATGATATCAATGGCAAGGTACGTCTG
GCTGGTTATAAAGAAGGCCTGAAAAAGAAAGGATTGCCTTTCAAAGAAGG
ACTTGTTTTTGAAGCTCAATATAAGTATCAAGAAGGATATCAGTTAGCGC
AGCGTGTCATTAATTCAGGCGCAACAGCTGCTTATGTTGCTGAGGATGAG
TTGGCTGCTGGGCTGTTAAATGGTTTGTTTGCTGCTGGTAAGAAAGTCCC
AGAAGACTTTGAAATTATCACGAGTAATGACTCTACTATTGCGCTTTATA
CTCGTCCTAATATGACATCTATCAGTCAGCCGATTTATGATTTAGGAGCA
GTTGCTATGCGCATGTTAACAAAGATTATGAACAAGGAAGAATTGGAAGA
AAAAGAAATTGTTCTTAATCATGGTATCAGAGAAAGAGGAACAACTAAAT
AG

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