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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1190 IGR1191 IGR1188 acpA,rncS, - SMu1376 pheS, - SMu1374 covS,vicK,csrS, - SMu1378 smc, - SMu1375 glnH, - SMu1381 acpA,rncS, - SMu1376 pheS, - SMu1374 covS,vicK,csrS, - SMu1378 smc, - SMu1375 glnH, - SMu1381 acpA,rncS, - SMu1376 pheS, - SMu1374 covS,vicK,csrS, - SMu1378 smc, - SMu1375 covR,vicR, - SMu1379 covX,vicX, - SMu1377 covR,vicR, - SMu1379 covX,vicX, - SMu1377 glnH, - SMu1381 glnQ, - SMu1380 glnQ, - SMu1380


LANL Gene ID: SMu1376

GenBank Locus Tag: SMU.1514c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
acpA  rncS  

Definition:
ribonuclease III

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1443949

Gene Stop:
1443254

Gene Length:
696

Molecular Weight*:
26014

pI*:
6.20

Net Charge*:
-3.39

EC:
3.1.26.3  

Functional Class:
Transcription; Degradation of RNA  

Gene Ontology:
Biological process
  GO:0006396    RNA processing
  GO:0016075    rRNA catabolic process

Cellular component
  GO:0005622    intracellular

Molecular function
  GO:0003723    RNA binding
  GO:0003725    double-stranded RNA binding
  GO:0004525    ribonuclease III activity


Pathway: pathway table

Secondary Evidence:
Nashimoto,H. and Uchida,H.
DNA sequencing of the Escherichia coli ribonuclease III gene and
its mutations
Molecular & general genetics : MGG. 201 (1), 25-29 (1985)
PubMed: 3903434

Takiff,H.E., Chen,S.M. and Court,D.L.
Genetic analysis of the rnc operon of Escherichia coli
Journal of bacteriology. 171 (5), 2581-2590 (1989)
PubMed: 2540151



Comment:
For other 'acp' genes see SMu0023 (ACP),(acpP); SMu1589 (acp) and SMu1671 (acpS).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Several matches in gapped BLAST to ribonuclease III: residues 1-227 are 63% similar to the enzyme in S.pyogenes (15674631|). Residues 1-226 are 61% similar to the protein from S.pneumoniae (15903170|).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0723 (5e-81).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379904|ref|NP_721859.1|  ribonuclease III [Streptococcu...   404   e-111
 gi|25010800|ref|NP_735195.1|  ribonuclease III [Streptococcu...   328   1e-88
 gi|22536887|ref|NP_687738.1|  ribonuclease III [Streptococcu...   327   4e-88
 gi|125718368|ref|YP_001035501.1|  Ribonuclease III, putative...   320   5e-86
 gi|50913818|ref|YP_059790.1|  ribonuclease III [Streptococcu...   319   7e-86
 gi|94989948|ref|YP_598048.1|  Ribonuclease III [Streptococcu...   319   7e-86
 gi|71903092|ref|YP_279895.1|  ribonuclease III [Streptococcu...   318   1e-85
 gi|15674631|ref|NP_268805.1|  ribonuclease III [Streptococcu...   318   2e-85
 gi|55821300|ref|YP_139742.1|  ribonuclease III [Streptococcu...   311   1e-83
 gi|116628032|ref|YP_820651.1|  dsRNA-specific ribonuclease [...   311   2e-83


InterPro Summary:  InterProScan

InterPro
IPR000999
Domain
Ribonuclease III
G3DSA:1.10.1520.10 [3-153]T 2.8000050426112498E-45 G3DSA:1.10.1520.10 RNase_III RNase_III
PF00636 [44-134]T 1.4000050690730898E-42 PF00636 Ribonuclease_3 Ribonuclease_3
SM00535 [20-155]T 1.19999999999993E-55 SM00535 RIBOc RIBOc
PS00517 [44-52]T 8.0E-5 PS00517 RNASE_3_1 RNASE_3_1
PS50142 [1-134]T 0.0 PS50142 RNASE_3_2 RNASE_3_2
SSF69065 [1-164]T 3.8E-48 SSF69065 RNase_III RNase_III
InterPro
IPR001159
Domain
Double-stranded RNA binding
PF00035 [161-227]T 1.29999924468183E-15 PF00035 dsrm dsrm
SM00358 [161-228]T 5.20000000000001E-22 SM00358 DSRM DSRM
PS50137 [160-229]T 0.0 PS50137 DS_RBD DS_RBD
InterPro
IPR011907
Family
Ribonuclease III, bacterial
TIGR02191 [8-227]T 5.6550851864086E-116 TIGR02191 RNaseIII RNaseIII
InterPro
IPR014720
Domain
Double-stranded RNA-binding-like
G3DSA:3.30.160.20 [160-227]T 3.499994668639491E-21 G3DSA:3.30.160.20 dsRNA-bd-like dsRNA-bd-like
noIPR
unintegrated
unintegrated
PTHR11207 [14-229]T 3.30001976117385E-80 PTHR11207 PTHR11207 PTHR11207
SSF54768 [147-228]T 4.9E-21 SSF54768 SSF54768 SSF54768


COGS Summary:  COGS Search
BeTs to 13 clades of COG0571
COG name: dsRNA-specific ribonuclease
Functional Class: K
The phylogenetic pattern of COG0571 is ----YqvCebrhujgpolinx
Number of proteins in this genome belonging to this COG is 1
Blocks Summary:  Blocks Search
***** IPB000999 (Ribonuclease III family) with a combined E-value of 4.3e-56.
    IPB000999A    21-30
    IPB000999B    43-79
    IPB000999C    116-134
    IPB000999D    160-179
    IPB000999E    185-195
    IPB000999F    209-220


 ProDom Summary:  Protein Domain Search
Residues 6-83 are 62% similar to a (III RIBONUCLEASE HYDROLASE ENDONUCLEASE) protein domain (PD002508 which is seen in RNC_LACLA.

Residues 155-225 are 45% similar to a (RNA-BINDING III DOUBLE-STRANDED NUCLEAR HYDROLASE) protein domain (PD003300 which is seen in RNC_LACLA.


Paralogs:  Local Blast Search
SMu1376 has no significant similarity (blastp p-value < 1e-3) to any other gene in this genome.


Pfam Summary:  Pfam Search
Residues 44 to 134 (E-value = 2.8e-47) place SMu1376 in the Ribonuclease_3 family which is described as RNase3 domain (PF00636)
Residues 161 to 227 (E-value = 1.4e-23) place SMu1376 in the dsrm family which is described as Double-stranded RNA binding motif (PF00035)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
117  
133

Top PDB Hits:
No significant hits to the NCBI PDB database.

Gene Protein Sequence:
MKTLEKKLAEDFKIVFSDKELLETAFTHTSYANEHRLLNISHNERLEFLG
DAVLQLTISHYLFDKYPQKAEGDLSKMRSMIVREESLAGFSRNCHFDRYI
KLGKGEEKSGGRQRDTILGDLFEAFLGALLLDAGLKAVEAFLNQVVIPKV
ENNNYERVTDYKTALQELLQVDGDVLIDYEVLKESGPAHAKCFEVAVSMN
HEKLSSGTGKSKKLAEQEAAKNALEKLQRGS

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAAACATTAGAAAAAAAACTGGCAGAAGACTTTAAGATCGTCTTTTC
TGACAAGGAATTATTGGAAACTGCCTTTACTCATACTAGTTATGCTAATG
AGCATCGCCTCCTAAACATTTCACATAACGAGCGCTTGGAATTTTTAGGA
GACGCTGTTCTGCAGTTAACGATTTCACATTATCTTTTTGACAAATACCC
TCAAAAAGCTGAAGGTGATTTATCAAAAATGCGTTCGATGATTGTTCGTG
AAGAAAGTTTGGCGGGTTTTTCTAGAAATTGTCACTTTGACCGCTATATT
AAATTAGGTAAGGGTGAAGAAAAATCTGGCGGTCGTCAAAGAGATACTAT
TTTGGGCGATTTATTTGAGGCTTTTTTAGGTGCTCTTTTATTGGATGCAG
GTCTTAAAGCTGTTGAGGCCTTTCTCAATCAAGTTGTGATTCCTAAGGTT
GAAAATAATAATTATGAACGTGTTACCGATTATAAAACGGCACTTCAAGA
ATTATTGCAAGTTGACGGGGATGTTTTGATCGATTACGAGGTCTTAAAAG
AATCAGGACCCGCCCATGCAAAATGCTTTGAAGTTGCTGTTTCTATGAAT
CATGAAAAACTTAGTAGCGGGACAGGCAAATCAAAAAAATTAGCAGAGCA
AGAGGCTGCTAAAAATGCGCTTGAAAAACTTCAACGAGGTTCTTAA

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