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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1175 IGR1173 IGR1172 IGR1174 IGR1176 IGR1177 IGR1179 IGR1171 IGR1178 lacF,celC, - SMu1357 lacA, - SMu1361 lacB,rpiB, - SMu1360 lacR, - SMu1362 lacX,galM, - SMu1354 lacC, - SMu1359 lacD, - SMu1358 lacG, - SMu1355 lacE, - SMu1356 rexA, - SMu1363 lacF,celC, - SMu1357 lacA, - SMu1361 lacB,rpiB, - SMu1360 lacR, - SMu1362 lacX,galM, - SMu1354 lacC, - SMu1359 lacD, - SMu1358 lacG, - SMu1355 lacE, - SMu1356 rexA, - SMu1363 Type: tandem, Name: SMu-TR-24 - 24 Type: tandem, Name: SMu-TR-24 - 24 lacF,celC, - SMu1357 lacA, - SMu1361 lacB,rpiB, - SMu1360 lacR, - SMu1362 lacX,galM, - SMu1354 lacC, - SMu1359 lacD, - SMu1358 lacG, - SMu1355 lacE, - SMu1356 rexA, - SMu1363


LANL Gene ID: SMu1358

GenBank Locus Tag: SMU.1493c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
lacD  

Definition:
tagatose-1,6-bisphosphate aldolase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1418686

Gene Stop:
1417709

Gene Length:
978

Molecular Weight*:
36477

pI*:
4.90

Net Charge*:
-9.39

EC:
4.1.2.40  

Functional Class:
Energy metabolism; Sugars  

Gene Ontology:
Biological process
  GO:0008152    metabolic process
  GO:0019512    lactose catabolic process via tagatose-6-phosphate

Molecular function
  GO:0003824    catalytic activity
  GO:0009024    tagatose-6-phosphate kinase activity
  GO:0016829    lyase activity


Pathway: pathway table
Galactose metabolism

Primary Evidence:
Rosey,E.L. and Stewart,G.C.
Nucleotide and deduced amino acid sequences of the lacR, lacABCD,
and lacFE genes encoding the repressor, tagatose 6-phosphate gene
cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans
J. Bacteriol. 174 (19), 6159-6170 (1992)
PubMed: 1400164


Secondary Evidence:
de Vos,W.M., Boerrigter,I., van Rooyen,R.J., Reiche,B. and
Hengstenberg,W.
Characterization of the lactose-specific enzymes of the
phosphotransferase system in Lactococcus lactis
J. Biol. Chem. 265 (36), 22554-22560 (1990)
PubMed: 2125052

van Rooijen,R.J., van Schalkwijk,S. and de Vos,W.M.
Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis
J. Biol. Chem. 266 (11), 7176-7181 (1991)
PubMed: 1901863


Comment:
For other 'lac' genes see SMu0104 (lacD); SMu0793 (lacR); SMu0794 (lacC); SMu0094 (lacI); SMu1359 (lacC);SMu1354 (lacX); SMu1355 (lacG); SMu1356 (lacE);SMu1357 (lacF);SMu1360 (lacB); SMu1361 (lacA);SMu1362 (lacR) and SMu1451 (lacE).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to tagatose-1,6-bisphosphate aldolase :residues 1-325 are 99% similar to the previously published protein in S.mutans (9622923|),(322120|) and (153676|).

Residues 1-325 are 82% similar to the enzyme from S.pneumoniae
(15903116|) and are 78% similar to enzyme from Lactococcus lactis (125924|).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1928 (1e-148).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379886|ref|NP_721841.1|  tagatose 1,6-diphosphate aldol...   631   e-179
 gi|153676|gb|AAA26907.1|  tagatose 1,6-aldolase                   629   e-179
 gi|157075794|gb|ABV10477.1|  tagatose 1,6-diphosphate aldola...   547   e-154
 gi|125718496|ref|YP_001035629.1|  Tagatose 1,6-diphosphate a...   547   e-154
 gi|25011375|ref|NP_735770.1|  tagatose 1,6-diphosphate aldol...   534   e-150
 gi|157074450|gb|ABV09133.1|  tagatose 1,6-diphosphate aldola...   529   e-148
 gi|148985796|ref|ZP_01818924.1|  tagatose 1,6-diphosphate al...   526   e-148
 gi|148995097|ref|ZP_01824004.1|  tagatose 1,6-diphosphate al...   526   e-147
 gi|15903116|ref|NP_358666.1|  tagatose 1,6-diphosphate aldol...   524   e-147
 gi|111657527|ref|ZP_01408269.1|  hypothetical protein SpneT_...   523   e-147


InterPro Summary:  InterProScan

InterPro
IPR002915
Family
Deoxyribose-phosphate aldolase/phospho-2-dehydro-3-deoxyheptonate aldolase
PF01791 [21-283]T 4.500010229032809E-108 PF01791 DeoC DeoC
InterPro
IPR005927
Family
Tagatose 1,6-diphosphate aldolase, Gram positive
TIGR01232 [2-325]T 0.0 TIGR01232 lacD lacD
InterPro
IPR013785
Domain
Aldolase-type TIM barrel
G3DSA:3.20.20.70 [4-324]T 1.1999906342183802E-106 G3DSA:3.20.20.70 Aldolase_TIM Aldolase_TIM
noIPR
unintegrated
unintegrated
SSF51569 [6-322]T 1.1E-75 SSF51569 SSF51569 SSF51569


COGS Summary:  COGS Search
No hits to the COGs database.
Blocks Summary:  Blocks Search
No significant hits to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 1-286 are 80% similar to a (ALDOLASE LYASE D-TAGATOSE-16-BISPHOSPHATE) protein domain (PD401329 which is seen in LACD_LACLA.

Residues 288-325 are 97% similar to a (ALDOLASE LYASE D-TAGATOSE-16-BISPHOSPHATE) protein domain (PD024298 which is seen in LACD_STRMU.

Residues 11-285 are identical to a (ALDOLASE TAGATOSE-BISPHOSPHATE LYASE) protein domain (PD017729 which is seen in LACD_STRMU.


Paralogs:  Local Blast Search
SMu1358 is paralogously related (blast p-value < 1e-3) to SMu0104, a predicted tagatose 1,6-aldolase.


Pfam Summary:  Pfam Search
Residues 6 to 325 (E-value = 4.5e-236) place SMu1358 in the LacD family which is described as Tagatose 1,6-diphosphate aldolase, (LacD) (PF04274)

 Top PDB Hits:
No significant hits to the NCBI PDB database.

Gene Protein Sequence:
MTLTQEKRSYMEKLSDENGIISALAFDQRGALKRLMAQYQTQEPTIAQME
ELKVLVAEELTPYASSMLLDPEYGLPAAKHLDKNAGLLLAYEKTGYDTTS
TKRLPDCLVEWSAKRLKKQGADAVKFLLYYDVDGDEEVNQQKQAYIERIG
SECKAEDIPFFLEILAYDETITDAASVEYAKVKPHKVLDAMKVFSDERFG
IDVLKVEVPVNMKYVEGFGDGPIVHTQDQAANFFKQQDQATPLPYIYLSA
GVSAKLFQDTLVFAKESGANFNGVLCGRATWAGSVKDYIEKGEAAARQWL
RTEGFKNIDELNKVLKATATSWKER

Gene Nucleotide Sequence:  Sequence Viewer
ATGACATTAACACAAGAAAAGCGCAGTTATATGGAAAAACTTAGTGATGA
AAATGGAATTATTTCAGCTTTAGCTTTTGACCAGCGTGGTGCTTTAAAAC
GCTTGATGGCGCAGTATCAAACGCAAGAACCAACGATTGCTCAAATGGAA
GAGCTGAAGGTCTTAGTAGCAGAAGAATTAACACCTTATGCTTCATCCAT
GCTGCTTGATCCAGAATATGGTCTTCCAGCAGCAAAACATTTGGATAAAA
ATGCAGGTTTGCTCCTTGCTTATGAGAAGACTGGTTATGATACAACAAGC
ACTAAACGCTTGCCAGATTGTCTGGTTGAATGGTCAGCCAAACGTTTGAA
AAAACAAGGTGCAGATGCTGTTAAATTCTTGCTCTATTATGATGTTGATG
GTGATGAAGAAGTTAACCAGCAAAAACAGGCTTATATTGAACGAATTGGT
TCTGAATGTAAGGCAGAAGATATTCCCTTTTTCCTTGAAATTTTAGCTTA
TGACGAAACCATTACTGATGCCGCAAGCGTTGAGTATGCTAAAGTAAAGC
CTCATAAAGTGCTTGATGCTATGAAAGTCTTCTCAGACGAGCGTTTTGGC
ATTGATGTTCTGAAGGTTGAGGTTCCTGTTAACATGAAGTATGTCGAAGG
TTTTGGTGATGGCCCAATTGTTCACACTCAAGATCAAGCAGCAAACTTCT
TTAAACAACAAGATCAAGCAACACCGCTTCCCTATATTTATTTGAGTGCA
GGTGTTTCTGCTAAGTTATTCCAAGATACGCTTGTTTTTGCAAAAGAGTC
AGGGGCTAATTTTAACGGTGTCCTTTGCGGACGTGCCACATGGGCTGGTT
CAGTTAAAGATTATATTGAAAAAGGCGAAGCAGCAGCCCGCCAGTGGCTT
CGTACAGAAGGATTCAAGAATATTGATGAGCTGAATAAGGTTTTAAAAGC
AACCGCAACGTCTTGGAAAGAAAGATAG

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